[Show abstract][Hide abstract] ABSTRACT: One hundred and one methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were classified into 10 genotypes based on their polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) coa pattern. PCR-RFLP coa patterns correlated with the clonal complex (CC) with the exception of CC5, which was related to 2 patterns (B and E). The PCR-RFLP coa gene technique provides a useful preliminary method to monitor variations in MRSA populations.
Scandinavian Journal of Infectious Diseases 08/2014; · 1.64 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Background: CA-MRSA is still a rare cause of MRSA infections in Spain, most of CA-MRSA has been isolated from Latin American immigrants. The expression of Panton-Valentine leucocidin (PVL) has been associated with CA-MRSA. The PVL toxin is encoded by lukS-PV-lukF-PV genes, which are carried on a bacteriophage. Four different PVL-carrying phages have been identified. Methods: One hundred and eight MRSA were isolated in the Northwest of Spain from 2005 to 2006. Documented clinical histories for all patients were obtained.The presence of mecA gene was confirmed in all MRSA isolates. The isolates were studied by analysis of the RFLP of the coagulase gen patterns. The SCCmec and the agr types were determined by multiplex and duplex PCR respectively. All the CA-MRSA strains were analyzed by MLST.
The presence of pvl genes was identified by PCR. The four PVL-carrying phages were studied by PCR in all PVL-positive strains.
Results: Five (4.6%) CA-MRSA infections were identified. The patients were native Spanish population. Four patients developed skin and soft-tissue infections and one otitis media.
The CA-MRSA strains were unrelated by RFLP with the known local epidemic clonal lineages (ST239, ST5, and ST36). Only these five strains were PVL positive.The five CA-MRSA strains harbored the PVL-carrying prophage Phi 108PVL.
Four strains belonged to ST8, SCCmec-IV, agr-1 and one strain belonged to ST30, SCCmec-IV, agr-3. Conclusions: The isolates belong at two of the six PVL-carrying MRSA genotypes worldwide widespread, ST8 (USA300 clone) and ST30 (Southwest Pacific Clone). The five CA-MRSA strains carried Phi 108PVL, being to our knowledge the first time that this prophage is identified in the ST8 clone. It seems that this phage is inactive, therefore further studies are warranted to understand the origin of PVL-positive MRSA.
Infectious Diseases Society of America 2008 Annual Meeting; 10/2008