Ingrid Zitňanová

Comenius University in Bratislava, Bratislava, Bratislavsky Kraj, Slovakia

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Publications (3)6.8 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: Abstract Crohn's disease (CD) is a nonspecific, chronic inflammatory disease of the gastrointestinal tract. It is supposed that in etiopathogenesis oxidative stress plays a role. However, its precise role in the active and non-active state of disease is not known yet. We conducted a pilot study focusing on the relationship between oxidative stress of Crohn´s disease in remission and the possibility to influence clinical parameters and markers of oxidative stress by polyphenolic extract, Pycnogenol(®). Compared to 15 healthy controls 15 pediatric CD patients (all were in remission according to their disease activity index - PCDAI) had reduced activity of Cu/Zn-superoxide dismutase (SOD) and increased oxidative damage to proteins. We found negative correlations between markers of inflammation (CRP, calprotectin) as well as between PCDAI and total antioxidant capacity. Activities of antioxidant enzymes, SOD and glutathione peroxidase (GPX) negatively correlated with calprotectin and PCDAI. Pycnogenol (2 mg/kg) positively influenced parameters of oxidative stress in CD patients after 10 weeks of administration.
    Free radical research 05/2013; · 2.22 Impact Factor
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    ABSTRACT: This study was focused on the monitoring how the anti-inflammatory substance, N(1)-methylnicotinamide (MNA), could influence oxidation and glycooxidation stress markers in rats under conditions of streptozotocin (STZ)-induced diabetes mellitus. Diabetes mellitus was induced in 60 male Wistar rats by intraperitoneal injection of STZ and after 7 days diabetic animals were allocated to five groups according to the dose of MNA administered for 7 weeks. The degree of DNA damage in lymphocytes, as well as advanced glycation endproducts (AGEs), protein carbonyls, lipid peroxides, and total antioxidant capacity (TEAC) in plasma were measured. Glycation damage to proteins (represented by AGEs level) was significantly increased in all diabetic groups compared to untreated non-diabetic animals. MNA did not affect TEAC of plasma in any group of diabetic rats. Supplementation of diabetic rats with MNA at the dose of 200 mg/kg resulted in decreased protein carbonyls (from 0.0818±0.0091 to 0.0558±0.0044 nmol/mg proteins; P<0.05, n=15) and DNA oxidation, reflected by the levels of 8-oxoG (0.6302±0.085 vs. 0.9213±0.108 8-oxoG/10(6) G; P<0.05, n=15), compared to untreated diabetic animals. Our results demonstrated that MNA at suitable concentrations could influence oxidative modifications of proteins and DNA.
    Redox report: communications in free radical research 01/2012; 17(1):1-7. · 1.51 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: In this study, we examined the relationships between perimenopausal symptoms, biochemical parameters, and markers of oxidative stress in women in perimenopause and compared them with those of premenopausal women. Sixty-two women (age, 53.2 ± 5.7 y) with perimenopausal symptoms were recruited to participate in our study. The control group consisted of 18 women without perimenopausal symptoms (age, 40 ± 5 y).Clinical perimenopausal symptoms were evaluated via the questionnaire of the Menopausal Rating Scale. Our participants were checked for basic biochemical parameters. The oxidative status of our samples was determined through the examination of lipoperoxides, 8-oxoguanine (8-oxoG) levels, and the total antioxidant status (TAS). Perimenopausal women had higher total cholesterol values and lower paraoxonase-1 (PON1) activity compared to reference values. Other biochemical parameters as well as 8-oxoG levels were unchanged compared with those of healthy control women. Lipoperoxide levels were significantly increased compared with those of premenopausal women. We found an indirect correlation between PON1 arylesterase (PON1 A) activity and lipoperoxide levels, between PON1 A activity and atherogenic index, between age and TAS, and between age and 8-oxoG levels. DNA repair ability and the total antioxidant status of women in perimenopause were significantly increased compared with women in premenopause. Hypercholesterolemic women had significantly increased low-density lipoprotein cholesterol levels when compared with normocholesterolemic individuals, but these values were still within the reference range. Normocholesterolemic women had significantly decreased high-density lipoprotein cholesterol levels, below the reference range. We found no correlations between perimenopausal symptoms and biochemical parameters or oxidative stress markers. We found that women in perimenopause are under increased oxidative stress manifested by reduced PON1 A activity and elevated lipoperoxidation, DNA repair ability, and TAS. Nutritional antioxidant supplementation may be an effective approach in improving menopausal symptoms.
    Menopause (New York, N.Y.) 09/2011; 18(11):1249-55. · 3.08 Impact Factor

Publication Stats

1 Citation
178 Views
6.80 Total Impact Points

Institutions

  • 2011
    • Comenius University in Bratislava
      • Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry
      Bratislava, Bratislavsky Kraj, Slovakia