Jieyun Zhang

Guangdong Medical College, Bao'an, Guangdong, China

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Publications (4)11.96 Total impact

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    ABSTRACT: Diagnosis of tuberculous pleurisy remains as challenge in the clinic. In this study, we evaluated the usefulness of a previous developed Mycobacterium tuberculosis antigen specific interferon-γ enzyme-linked immunospot (ELISPOT) assay in the diagnosis of tuberculous pleurisy by testing a cohort of 352 patients with pleural effusion. We found that M. tuberculosis antigen-specific interferon-γ-producing cells were enriched four to five times in pleural fluid as compared with parallel assays of peripheral blood in patients with tuberuclous pleurisy. The sensitivity, specificity, positive predictive value and negative predictive value of the pleural fluid mononuclear cell ELISPOT assay for the diagnosis of tuberculous pleurisy were 95.7%, 100%, 100%, 81.0%, respectively. In comparison, the sensitivity and specificity of ELISPOT assay using peripheral blood mononuclear cells were 78.3% and 86.3%, respectively. The sensitivity and specificity of pleural fluid adenosine deaminase activity test were 55.5% and 86.3%, respectively. These results demonstrate that, the M. tuberculosis antigen specific ELISPOT assay performed on pleural fluid mononuclear cells provides an accurate, rapid diagnosis of tuberculous pleurisy.
    Clinical and vaccine Immunology: CVI 01/2014; · 2.60 Impact Factor
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    ABSTRACT: IL-22 has been suggested to play an important role in immune response against Mycobacterium tuberculosis infection. However, the exact role of IL-22 in human tuberculosis (TB) infection remains unclear and the regulatory mechanism of IL-22 response in human TB is unknown. In this study, we observed that successful anti-tuberculosis treatment induced an enhanced and sustained M. tuberculosis antigen-specific IL-22 response, correlated with the decrease of the frequencies of CD19+CD5+CD1d+ regulatory B cells. We also found that depletion of CD19+ B cells significantly enhanced M. tuberculosis antigen-specific IL-22 production by peripheral blood mononuclear cells. More importantly, we observed that purified CD19+ B cells, and more efficiently, CD19+CD5+CD1d+ regulatory B cells, suppressed IL-22 production. In summary, we showed here for the first time that effective anti-tuberculosis treatment restores M. tuberculosis antigen-specific IL-22 response through a novel mechanism by reducing the frequencies of CD19+CD5+CD1d+ regulatory B cells in human TB.
    Tuberculosis (Edinburgh, Scotland) 01/2013; · 2.54 Impact Factor
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    ABSTRACT: Although the importance of B cells in the host immune response upon Mycobacterium tuberculosis infection has been recognized, a conclusive role for B cells has yet to be determined. In the present study, we found that primary CD19(+) B cells isolated from patients with tuberculosis significantly inhibited Th17, but not Th1, cell activation. Moreover, the suppressive activity was mediated by a CD19(+)CD1d(+)CD5(+) B cell population. Notably, patients with tuberculosis were found to have significantly higher frequencies of CD19(+)CD1d(+)CD5(+) B cells with stronger suppressive activity than such cells from healthy donors. Furthermore, the frequency of CD19(+)CD1d(+)CD5(+) B cells in peripheral blood was inversely correlated with that of Th17 cells in patients with tuberculosis. This finding that B cells negatively regulate Th17 responses provides a novel mechanism in the regulation of CD4(+) T cell responses-aside from regulatory T cells-during M. tuberculosis infection, which may impact the clinical outcome of tuberculosis.
    Cellular Immunology 02/2012; 274(1-2):89-97. · 1.74 Impact Factor
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    ABSTRACT: Recent studies showed that IL-22 plays a protective role in the host defense. However, the contribution of polymorphisms of the IL-22 gene to human TB susceptibility remains untested. We have designed a computational approach to select functional SNPs in the IL-22 gene and genotyped them in a two-stage case-control study in Chinese (479 cases and 358 controls). We found that rs2227473, an SNP in the promoter region of IL-22, is associated with TB susceptibility at both stages of our study. The SNP shows associations with p-values of 0.028 and 0.034 respectively, and a combined p-value of 0.0086, with odds ratio at 0.65 (95% confidence interval 0.45-0.90). We further validated the association with an independent cohort (413 cases and 241 controls in Chinese). Our functional studies showed that patients with A allele have significantly higher IL-22 expression than those without A allele under both non-specific and specific stimulations.
    Scientific Reports 01/2011; 1:20. · 5.08 Impact Factor