Publications (2)1.33 Total impact
Article: Clinical color intensity of white spot lesions might be a better predictor of enamel demineralization depth than traditional clinical grading.[show abstract] [hide abstract]
ABSTRACT: The aims of this study were to calculate the volume of white spot lesions by using microcomputed tomography and to determine which clinical attribute of the white spot lesion could better predict its volume: the clinically visible white spot lesion surface area or its color intensity. White spot lesions were induced in 8 patients in vivo on 23 healthy premolars destined for extraction during orthodontic treatment by using specially designed plaque-retaining orthodontic bands. After 7 weeks, the premolars were extracted. After extraction, the resulting white spot lesions were photographed and clinically graded. The teeth were analyzed with microcomputed tomography. After 7 weeks, 70% of the teeth developed clinical white spot lesions. Clinically, the size of the lesions varied from minor to severe. Their volumes varied from 0 to 1.2931 mm(3). The traditional grades for white spot lesions correlated significantly with color intensity. A significant correlation was found between white spot lesion color intensity and lesion volume. This correlation was found to be better than that between the white spot lesion clinical score and lesion volume. Our results indicate that white spot lesion color intensity might predict the depth of enamel demineralization as well as or better than traditional white spot lesion scoring. Therefore, the dentist could use this information when planning treatment for white spot lesions.American journal of orthodontics and dentofacial orthopedics: official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 08/2012; 142(2):191-8. · 1.33 Impact Factor
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ABSTRACT: Dental caries is one of the most common diseases in the world. However, our understanding of how the microbial community composition changes in vivo as caries develops is lacking. An in vivo model was used in a longitudinal cohort study to investigate shifts in the microbial community composition associated with the development of enamel caries. White spot lesions were generated in vivo on human teeth predetermined to be extracted for orthodontic reasons. The bacterial microbiota on sound enamel and on developing carious lesions were identified using the Human Oral Microbe Identification Microarray (HOMIM), which permits the detection of about 300 of the approximate 600 predominant bacterial species in the oral cavity. After only seven weeks, 75% of targeted teeth developed white spot lesions (8 individuals, 16 teeth). The microbial community composition of the plaque over white spot lesions differed significantly as compared to sound enamel. Twenty-five bacterial taxa, including Streptococcusmutans, Atopobiumparvulum, Dialisterinvisus, and species of Prevotella and Scardovia, were significantly associated with initial enamel lesions. In contrast, 14 bacterial taxa, including species of Fusobacterium, Campylobacter, Kingella, and Capnocytophaga, were significantly associated with sound enamel. The bacterial community composition associated with the progression of enamel lesions is specific and much more complex than previously believed. This investigation represents one of the first longitudinally-derived studies for caries progression and supports microbial data from previous cross-sectional studies on the development of the disease. Thus, the in vivo experiments of generating lesions on teeth destined for extraction in conjunction with HOMIM analyses represent a valid model to study succession of supragingival microbial communities associated with caries development and to study efficacy of prophylactic and restorative treatments.Journal of Oral Microbiology 01/2012; 4.