R B Hazan

Publications (2)12.75 Total impact

• Article: p21CIP1 mediates reciprocal switching between proliferation and invasion during metastasis.

  X Qian, J Hulit, K Suyama, E A Eugenin, T J Belbin, O Loudig, T Smirnova, Z N Zhou, J Segall, J Locker, G R Phillips, L Norton, R B Hazan
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  ABSTRACT: Cell proliferation and invasion are critical for malignant progression, yet how these processes relate to each other and whether they regulate one another during metastasis is unknown. We show that invasiveness of breast cancer cells is associated with growth arrest due to p21CIP1 upregulation. Knockdown of p21CIP1 increases cell proliferation and suppresses invasion. Since p21CIP1 acts to inhibit cyclin E during cell-cycle progression, we demonstrated that a constitutively active form of cyclin E had similar effects to p21CIP1 inhibition resulting in enhanced cell growth and suppressed invasiveness. We tested these findings in vivo in the Polyoma middle T mammary tumor model in which p21CIP1 was deleted. p21CIP1 knockout mice exhibited dramatic suppression of metastasis, independent of tumor growth, which was rescued by p21CIP1. Metastasis suppression by p21CIP1 ablation was associated with striking cytoskeletal reorganization leading to a non-invasive and highly proliferative state. Thus, p21CIP1 regulates metastasis by mediating reciprocal switching between invasion and proliferation.Oncogene advance online publication, 2 July 2012; doi:10.1038/onc.2012.249.
  Oncogene 07/2012; · 6.37 Impact Factor
• Article: N-cadherin regulates mammary tumor cell migration through Akt3 suppression.

  S Chung, J Yao, K Suyama, S Bajaj, X Qian, O D Loudig, E A Eugenin, G R Phillips, R B Hazan
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  ABSTRACT: N-cadherin is a cell-cell adhesion molecule that plays a role in breast cancer metastasis. Here, we show that in vivo expression of N-cadherin in the PyMT mouse model, which enhances mammary tumor metastasis, results in selective inhibition of Akt3 expression and phosphorylation. Similarly, exogenous expression of N-cadherin in PyMT or MCF-7 mammary tumor cells enhanced cell motility and caused a dramatic reduction in Akt3 expression and phosphorylation. Moreover, knockdown of Akt3 in PyMT tumor cells increased cell motility and disrupted mammary morphogenesis, but had no effect on cell proliferation. Conversely, overexpression of wild-type Akt3 in PyMT-N-cadherin cells inhibited cell motility promoted by N-cadherin. Taken altogether, these findings demonstrate that N-cadherin suppresses Akt3 to promote cell motility and highlight the intricate regulation of Akt isoforms by N-cadherin during metastasis.Oncogene advance online publication, 12 March 2012; doi:10.1038/onc.2012.65.
  Oncogene 03/2012; · 6.37 Impact Factor