Madhuri Chandrakant Pagariya

Publications (3)7.04 Total impact

• Article: Identification of Water Deficit Stress Upregulated Genes in Sugarcane

  Gajjeraman Prabu, Prashant Govindrao Kawar, Madhuri Chandrakant Pagariya, Doddananjappa Theertha Prasad
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  ABSTRACT: Sugarcane represents an important renewable source among biofuel crops with high capability to assimilate carbon among the C4 plants. Limited availability of freshwater renders this crop uneconomical, warranting the necessity for the development of varieties with higher water use efficiency and tolerant to water deficiency stress. Sugarcane variety cv. Co740 was subjected to varied levels of water deficiency stress to isolate transcripts differentially expressed to the imposed stress. The leaf relative water content was used as a measure to estimate the stress response. PCR-based cDNA suppression subtractive hybridization technique was applied to construct forward subtracted library for differentially expressed genes under stress. Dot blot-selected 158 clones showing elevated response were sequenced, of which 62% resembled similarity with known functional genes, 12% with hypothetical proteins of plant origin, while 26% represented new unknown sequences. Annotation of these differentially expressed sequence tags (ESTs) in the moderately water deficit stress-tolerant cultivar predicted that most of them encoded proteins involved in cellular organization, protein metabolism, signal transduction, and transcription. Further, semi-quantitative reverse transcriptase PCR carried out for five genes projected the involvement of these ESTs in stress alleviation/tolerance. Results from this study may help in targeting useful genes for improving drought tolerance in sugarcane and other grasses. KeywordsWater deficit stress–Sugarcane–RWC–SSH–sqRT-PCR–Drought tolerance
  Plant Molecular Biology Reporter 04/2012; 29(2):291-304. · 2.45 Impact Factor
• Article: Biochemical characterization and identification of differentially expressed candidate genes in salt stressed sugarcane.

  Madhuri Chandrakant Pagariya, Rachayya Mallikarjun Devarumath, Prashant Govindrao Kawar
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  ABSTRACT: Sugarcane productivity is worldwide subjected to increasing environmental constraints, predominantly to drought and salinity owing to their high magnitude of impact and wide distribution. The present study provides insights into the knowledge of sugarcane responses at germination, tillering and respective recovery stages to high salinity at physio-biochemical and molecular level. Our results indicated that there was a negative relationship between catalase, and peroxidase activity with lipid peroxidation and SOD activity. Increase in MDA and SOD levels at the earlier stages of stress and later increase in CAT and POD levels on prolonged stress was evidenced. Thus they can be used as indicators of stress for sugarcane plants facing unfavourable environmental conditions. At molecular level, we have identified 137 salinity tolerant candidate cDNAs from sugarcane by cDNA-SSH, representing 20% of which are novel sugarcane genes. These unique sequences, never reported elsewhere to be stress related and might provide further understanding on perception, response and adaptations mechanisms of the non-model plant like sugarcane to salinity stress and will be potential candidates for manipulating salt tolerant trait.
  Plant Science 03/2012; 184:1-13. · 2.94 Impact Factor
• Article: Physio-biochemical analysis and transcript profiling of Saccharum officinarum L. submitted to salt stress

  Madhuri Chandrakant Pagariya, M. Harikrishnan, Pranali Arun Kulkarni, Rachayya Mallikarjun Devarumath, Prashant Govindrao Kawar
  Acta Physiologiae Plantarum 07/2011; 33(4):1411-1424. · 1.64 Impact Factor