H. Trabelsi

University of Sfax, Şafāqis, Şafāqis, Tunisia

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Publications (33)17.59 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Trichophyton interdigitale is the second most frequent cause of superficial fungal infections in various parts of the human body. Studying the population structure and genotype differentiation of T. interdigitale strains may lead to significant improvements in clinical practice. The present study aimed to develop and select suitable variable number tandem repeat (VNTR) markers for 92 clinical strains of T. interdigitale. Based on four VNTR markers analysis, four to eight distinct alleles were detected for each marker. The marker with the highest discriminatory power had eight alleles and a D value of 0.802. The combination of all four markers yielded a 0.969 D value with 29 distinct multilocus genotypes. VNTR typing revealed a genetic diversity among strains, identifying into three populations according to their colonization sites. A correlation between phenotypic characteristics and the multilocus genotypes was observed. Seven patients harbored T. interdigitale strains with different genotypes. Typing of clinical T. interdigitale samples by VNTR markers displayed an excellent discriminatory power and 100% reproducibility.
    Journal of clinical microbiology. 07/2014;
  • Journal francais d'ophtalmologie 05/2014; · 0.51 Impact Factor
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    ABSTRACT: The Trichophyton mentagrophytes complex is the main cause of superficial mycoses in humans and animals. Molecular research has provided useful insights into the taxonomy of this complex to overcome the challenges with conventional diagnostics. The aim of this study was to identify, type and differentiate anthropophilic and zoophilic species of the T. mentagrophytes complex. Sixty clinical samples identified as T. mentagrophytes by morphological characteristics were isolated using polymerase chain reaction-restriction fragment length polymorphism and sequence analysis of the internal transcribed spacer (ITS) regions. The identification of our strains by conventional methods was confirmed using polymerase chain reaction (PCR) sequencing in 93.34% of the cases. The strains under investigation were recategorised as T. rubrum (Tr2711). In addition, PCR products were independently digested with the restriction endonucleases, MvaI and HinfI, to produce a single dominant profile for T. interdigitale. ITS sequence analysis revealed a polymorphism in the ITS1 and 5.8S regions. Analysis of the consensus sequences distinguished four types of genotypes among our T. interdigitale species. Moreover, ITS type I was the dominant genotype characterising the anthropophilic variant of T. interdigitale. The phylogenetic study showed that only 5% of our strains were zoophilic. PCR sequencing was useful for distinguishing anthropophilic and zoophilic species of T. interdigitale, in which the differentiation is relevant because it helps to prescribe the correct treatment and to identify the surrounding source of infection.
    Mycoses 03/2014; · 1.28 Impact Factor
  • Journal francais d'ophtalmologie 01/2014; · 0.51 Impact Factor
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    ABSTRACT: Invasive fungal infections are a major complication and an important cause of morbidity and mortality among solid organ transplant recipients. Their diagnosis is difficult and their prognosis is often pejorative. The aim of this study was to report the cases of invasive fungal infections in renal transplant recipients in Habib Bourguiba Sfax university hospital and to identify the main fungal agents. It is a retrospective study of invasive fungal infections in renal transplant recipient reported in our hospital from January 1995 to February 2013. Invasive fungal infections were diagnosed in 11 cases (3.4%) among 321 renal transplant recipients. These infections included four cases of pneumocystosis, two cases of candidiasis, two cases of aspergillosis, two cases of cryptococcosis and one case of mucormycosis. There were six men and five women. The mean age was 37 years. The infection was late in 63% of cases (>3 months after transplantation). The prolonged corticosteroid and immunosuppressive therapy were the main risk factors (100%) followed by renal failure (45%), graft rejection (45%), broad spectrum antibiotics (45%), CMV infection (36%), neutropenia (36%) and dialysis (18%). The evolution under treatment was favourable only in two cases (18%). Invasive fungal infections are not common among kidney transplant recipients. However, they remain an important cause of morbidity and mortality in this group of patients. Prevention, early diagnosis and appropriate management are necessary to improve prognosis and reduce mortality rate.
    Journal de Mycologie Médicale/Journal of Medical Mycology 09/2013; · 0.74 Impact Factor
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    ABSTRACT: The genus Fusarium, initially known for its important agro-economic impact, is more and more often implicated in human pathology. In fact, multiples allergic, toxic and infectious manifestations are more reported in immunocompetent and immunocompromised hosts. The objective of our study was to analyse the epidemiological, mycological and clinical features of fusariosis reported in our CHU. Eighty-seven cases of Fusarium infections were collected: 34 cases of onychomycosis (39%), 26 cases of intertrigos (30%), 25 cases of keratomycosis (29%), one case of atypical invasive fusariosis due to Fusarium oxysporum species complex (FOSC) and one case of localized gingivolabial fusariosis due to Fusarium solani species complex (FSSC) in a patient with leukemia in phase of deep bone marrow aplasia, whose outcome was favorable after exiting of aplasia period and a treatment by amphotericine B. The case of pseudotumoral cutaneous fusariosis to F. oxysporum complicated with osteolysis and septic arthritis occurred in a pregnant woman without any immune deficit. The evolution was fatal in spite of prescription of multiple systemic antifungals. Concerning keratomycosis, Fusarium was the first agent responsible for these infections (43%). The corneal traumatism was found in 37.5% of cases and FSSC was the most isolated (72%). For superficial dermatomycosis, Fusarium was the third agent of onychomycosis in molds (25%). The most isolated species were FSSC (68%) and FOSC (20%). The intertrigo frequency was 0.07% and they were mostly caused by FSSC (84%) and FOSC (16%). Fusarium is an important cause of mold infections in our region. So, the species identification is useful because some species are resistant to the most common systemic antifungal agents.
    Journal de Mycologie Médicale / Journal of Medical Mycology. 06/2013; 23(2):130–135.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The genus Fusarium, initially known for its important agro-economic impact, is more and more often implicated in human pathology. In fact, multiples allergic, toxic and infectious manifestations are more reported in immunocompetent and immunocompromised hosts. The objective of our study was to analyse the epidemiological, mycological and clinical features of fusariosis reported in our CHU. Eighty-seven cases of Fusarium infections were collected: 34 cases of onychomycosis (39%), 26 cases of intertrigos (30%), 25 cases of keratomycosis (29%), one case of atypical invasive fusariosis due to Fusarium oxysporum species complex (FOSC) and one case of localized gingivolabial fusariosis due to Fusarium solani species complex (FSSC) in a patient with leukemia in phase of deep bone marrow aplasia, whose outcome was favorable after exiting of aplasia period and a treatment by amphotericine B. The case of pseudotumoral cutaneous fusariosis to F. oxysporum complicated with osteolysis and septic arthritis occurred in a pregnant woman without any immune deficit. The evolution was fatal in spite of prescription of multiple systemic antifungals. Concerning keratomycosis, Fusarium was the first agent responsible for these infections (43%). The corneal traumatism was found in 37.5% of cases and FSSC was the most isolated (72%). For superficial dermatomycosis, Fusarium was the third agent of onychomycosis in molds (25%). The most isolated species were FSSC (68%) and FOSC (20%). The intertrigo frequency was 0.07% and they were mostly caused by FSSC (84%) and FOSC (16%). Fusarium is an important cause of mold infections in our region. So, the species identification is useful because some species are resistant to the most common systemic antifungal agents.
    Journal de Mycologie Médicale/Journal of Medical Mycology 05/2013; · 0.74 Impact Factor
  • Source
    Journal de Mycologie Médicale / Journal of Medical Mycology. 03/2013; 23(1):77–78.
  • Journal de Mycologie Médicale / Journal of Medical Mycology. 03/2013; 23(1):82.
  • Journal de Mycologie Médicale/Journal of Medical Mycology 03/2013; 23(1):82. · 0.74 Impact Factor
  • Journal de Mycologie Médicale / Journal of Medical Mycology. 03/2013; 23(1):81.
  • H. Trabelsi, A. Chbeb, A. Sellami
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    ABSTRACT: In this paper a discrete-time model for Permanent Magnet Synchronous Motor (PMSM) based on the Explicit Euler method is presented. Thus, an advanced application of the discrete variable structure control theory using a discrete time varying sliding surface is designed for the tracking of the rotor velocity. First, modeling and simulation of the PMSM supplied by an inverter is presented. Then, closed loop simulations of the RST control and the discrete time sliding mode control (DSMC) are realized. The obtained results from the two approaches are finally compared in terms of good performances.
    Electrical Engineering and Software Applications (ICEESA), 2013 International Conference on; 01/2013
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    ABSTRACT: In recent years, an increase of systemic Candida infections was noted. Thus, identification and susceptibility testing to antifungals became of considerable importance. The technique of dilution in liquid medium developed by « National committee for clinical laboratory standards » NCCLS or more recently named CLSI « Clinical and laboratory standards institute » is the reference method most used. The European committee "European committee on antibiotic susceptibility testing" or EUCAST has made progress by determining the susceptibility of strains within a shorter time. However, the use of these techniques is limited especially in clinical microbiology laboratories. Indeed, others techniques for determining the sensitivity have been developed such as those based on agar diffusion (E-test and disk diffusion), on microdilution (Sensititre yeastOne, Vitek 2 AST-YS01), on flow cytometry techniques and the MALDI-TOF.
    Annales de biologie clinique. 12/2012; 70(6):635-642.
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    ABSTRACT: Acanthamoeba keratitis (AK) is a sight-threatening infection. We report five cases of AK diagnosed from 2005 to 2009 in the Laboratory of Parasitology-Mycology at Habib Bourguiba Sfax Hospital, Tunisia. All were associated with improper care of contact lenses (rinsing of contact lenses with tap water and inappropriate cleaning) and lens storage. The patients displayed different clinical presentations: corneal inflammation, corneal ulceration, and corneal abscess. The diagnosis was made after direct examination, culture, and polymerase chain reaction amplification with specific primers. The genotype classification was based on the highly variable DF3 region in the 18S rRNA gene. This is the first study characterizing Acanthamoeba genotype in Tunisia and North Africa. All Acanthamoeba isolates were associated to the T4 genotype. Three different DF3 sequence types were related to AK infections T4/10, T4/15, and T4/16.
    Parasitology Research 10/2012; · 2.85 Impact Factor
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    ABSTRACT: Free-living amoebae are widely distributed in soil and water. Small number of them was implicated in human disease: Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris and Sappinia diploidea. Some of the infections were opportunistic, occurring mainly in immunocompromised hosts (Acanthamoeba and Balamuthia encephalitis) while others are non opportunistic (Acanthamoeba keratitis, Naegleria meningoencephalitis and some cases of Balamuthia encephalitis). Although, the number of infections caused by these amoebae is low, their diagnosis was still difficult to confirm and so there was a higher mortality, particularly, associated with encephalitis. In this review, we present some information about epidemiology, ecology and the types of diseases caused by these pathogens amoebae.
    Pathologie Biologie 04/2012; · 1.67 Impact Factor
  • Journal de Mycologie Médicale / Journal of Medical Mycology. 03/2012; 22(1):115.
  • Journal de Mycologie Médicale / Journal of Medical Mycology. 03/2012; 22(1):112.
  • Journal de Mycologie Médicale/Journal of Medical Mycology 03/2012; 22(1):112. · 0.74 Impact Factor
  • Journal de Mycologie Médicale/Journal of Medical Mycology 03/2012; 22(1):103–104. · 0.74 Impact Factor
  • Journal de Mycologie Médicale / Journal of Medical Mycology. 03/2012; 22(1):112–113.

Publication Stats

33 Citations
17.59 Total Impact Points

Institutions

  • 2012–2014
    • University of Sfax
      • • Faculty of Medicine of Sfax
      • • Department of Medicine A
      Şafāqis, Şafāqis, Tunisia
    • Faculty of Medecine of Tunis
      Tunis-Ville, Tūnis, Tunisia
  • 2010
    • Hedi Chaker Hospital
      Şafāqis, Şafāqis, Tunisia