Publications (2)1.8 Total impact
Article: The use of the microplate alamar blue assay (MABA) to assess the susceptibility of Mycobacterium lepraemurium to anti-leprosy and other drugs.[show abstract] [hide abstract]
ABSTRACT: Although murine leprosy is no longer a common illness, our understanding of the biology of this disease is incomplete. One particular example of this concerns the etiologic agent Mycobacterium lepraemurium (MLM). MLM is a fastidious microorganism that is difficult to grow in axenic media; in a way, this has hampered attempts to thoroughly study its physiological and metabolic characteristics. MLM is an obligate intracellular bacillus that invades macrophages and replicates profusely with a generation time that oscillates between 0.5 and 11 days. In the present study, we have successfully maintained MLM alive for more than 12 days in vitro, providing us with an opportunity to study its susceptibility to several anti-leprosy agents and other drugs. To achieve this, we used a fluorescence reduction assay of alamar blue (a resazurin) in a microplate format (microplate-alamar-blue-assay; MABA), which is a highly sensitive, practical, and inexpensive method for assaying cell viability. We found that MLM was highly susceptible to clofazimine and rifampicin and was less susceptible to streptomycin, thiacetazone, kanamycin, dapsone, and ethionamide, in that order. MLM was not susceptible to four plant triterpenoids (oleanolic acid, neolignan-c, sitosterol, and ursolic acid) for which bactericidal activity has been reported in M. tuberculosis. Because the MABA has high sensitivity, it can be used to monitor the activity of microorganisms that are difficult to cultivate (such as M. lepraemurium), in response to various drugs, thus offering a method to complement the study of murine leprosy, about which many questions remain unanswered.Journal of Infection and Chemotherapy 03/2012; · 1.80 Impact Factor
Article: Characterization of four soybean varieties grown on Mexican land: a preliminary analysis for a proteomic study[show abstract] [hide abstract]
ABSTRACT: Soybean is a source of high-quality proteins and fatty acids for animal and human nutrition. To satisfy the national demand of this crop it is necessary to increase the production of soybean by developing or selecting species that are resistant to adverse environmental conditions. Four soybean varieties customarily grown in Mexico were studied in a comparative manner. Each variety was characterized on the basis of impurities, grain integrity, water content, total and free fatty acids content, protein content, and germination capability following the methods and regulations indicated in NMX-FF-089-1994-SCFI, ISTA and AOAC. None of the soybean varieties presented impurities; Huasteca-100 soybean had a lower percentage of immature grains while the UFV-1 variety had the lowest number of grains damaged by microorganisms. Humidity contents were similar in all four soybean varieties (around 7%). The Huasteca-100 variety had some lower protein content while the UFV-1 variety had the maximum (39%). On the contrary, the lipid content was higher in Huasteca-100 and lower in the UFV-1 variety. Based on these results and on the fact that Huasteca 100 was a strong prospect for massive cultivation in Mexico, this variety was considered an adequate substrate to further study the effect of diverse stressing conditions.Archives of Agronomy and Soil Science 04/2010; 56(2):139-148.