Hui-Qiang Liu

Shanghai Institute of Applied Physics, Shanghai, Shanghai Shi, China

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Publications (2)5.84 Total impact

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    ABSTRACT: The primary goal of this study was to evaluate the feasibility of using anti-vascular endothelial growth factor receptor 2 (VEGFR2)-conjugated poly(lactic-co-glycolic acid) (PLGA) microspheres as an x-ray phase contrast agent to assess the VEGFR2 expression in cell cultures. The cell lines, mouse LLC (Lewis lung carcinoma) and HUVEC (human umbilical vein endothelial cell), were selected for cell adhesion studies. The bound PLGA microspheres were found to better adhere to LLC cells or HUVECs than unbound ones. Absorption and phase contrast images of PLGA microspheres were acquired and compared in vitro. Phase contrast imaging (PCI) greatly improves the detection of the microspheres as compared to absorption contrast imaging. The cells incubated with PLGA microspheres were imaged by PCI, which provided clear 3D visualization of the beads, indicating the feasibility of using PLGA microspheres as a contrast agent for phase contrast CT. In addition, the microspheres could be clearly distinguished from the wall of the vessel on phase contrast CT images. Therefore, the approach holds promise for assessing the VEGFR2 expression on endothelial cells of tumor-associated vessels. We conclude that PLGA microsphere-based PCI of the VEGFR2 expression might be a novel, promising biomarker for future studies of tumor angiogenesis.
    Physics in Medicine and Biology 04/2012; 57(10):3051-63. DOI:10.1088/0031-9155/57/10/3051 · 2.92 Impact Factor
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    ABSTRACT: Computed tomography combined with angiography has recently been developed to visualize three-dimensional (3D) vascular structure in experimental and clinical studies. However, there remain difficulties in using conventional x-ray angiography to detect small vessels with a diameter less than 200 μm. This study attempted to develop a novel method for visualizing the micro-angioarchitecture of rat spinal cord. Herein, synchrotron radiation-based x-ray in-line phase contrast computed tomography (IL-XPCT) was used to obtain 3D micro-vessel structure without angiography. The digital phase contrast images were compared with conventional histological sections. Our results clearly demonstrated that the resolution limit of the spatial blood supply network in the normal rat thoracic cord appeared to be as small as ~10 μm. The rendered images were consistent with that obtained from histomorphology sections. In summary, IL-XPCT is a potential tool to investigate the 3D neurovascular morphology of the rat spinal cord without the use of contrast agents, and it could help to evaluate the validity of the pro- or anti-angiogenesis therapeutic strategies on microvasculature repair or regeneration.
    Physics in Medicine and Biology 02/2012; 57(5):N55-63. DOI:10.1088/0031-9155/57/5/N55 · 2.92 Impact Factor