ABSTRACT: The aim of this study was to evaluate the effects of opium addiction on the secretion of IL-4, IFN-γ, IL-6 and TGF-β under In Vivo and In Vitro conditions. The blood samples were collected and PBMCs were cultured in RPMI1640 with and without opium for 48hours. The levels of the cytokines were measured using ELISA technique. The results showed that plasma levels of IL-4 and IFN-γ were significantly lower and IL-6 and TGF-β were higher in plasma taken from opium-addicted subjects. The concentrations of all the cytokines in opium addicted subjects inInVitrocondition were significantly lower than the control group. Addicted subjects cultured lymphocytes significantly decrease secreted IL-4, IL-6 and TGF-β but not IFN-γ in response to being cultured with opium, where as IFN-γ was increased in controls. These results may explain the frequent microbial infections and an increased tumor incidence seen in addicted patients.
Immunology letters 04/2013; · 2.91 Impact Factor
ABSTRACT: Type 2 diabetes mellitus is one of the most common types of endocrine disease and the immune system plays a predominant role in its pathogenesis.
The present study aimed to examine known gene polymorphisms within IL-12B (+1188) region and its circulating serum levels in Type 2 diabetic patients from the southeastern region of Iran and compare them with unrelated controls.
In this clinical study, peripheral blood was collected from 114 Type 2 diabetic patients and 100 healthy controls. Serum levels of IL-12B were measured by ELISA. Genomic DNA was extracted from peripheral blood samples and polymorphisms at the +1188 position of the IL-12B gene were assessed using PCR restriction fragment-length polymorphism.
Our findings demonstrated that the AA genotype and the A allele of IL-12B were increased significantly in Type 2 diabetic patients when compared with controls. Our results also showed that the circulating levels of IL-12B were significantly decreased in Type 2 diabetic patients when compared with controls.
According to the findings of the current study, we concluded that IL-12B and its +1188 polymorphism may play a prominent role in the pathogenesis of Type 2 diabetes. Further replicative investigations using a larger sample size are essential to identify additional IL-12B genetic variants associated with a risk of Type 2 diabetes.
Biomarkers in Medicine 02/2012; 6(1):89-95. · 0.86 Impact Factor
ABSTRACT: Cytokines are considered important factors for the pathogenesis of asthma as they play a key role in the regulation of immune responses. The aim of this study was to investigate the association between this disease and polymorphisms in the -592 region of the IL-10 gene.
This study was carried out on 100 asthmatic patients and 100 healthy controls. PCR-RFLP was applied to examine the polymorphisms in the -592 region of the IL-10 gene.
Our results showed a significant difference between patients and controls in terms of genotypes and alleles of the -592 region of the IL-10 gene.
According to our results, it can be concluded that the IL-10 promoter polymorphisms may play a crucial role in the pathogenesis of asthma.
Clinical laboratory 01/2012; 58(3-4):267-71. · 0.90 Impact Factor
Biochemistry and Analytical Biochemistry. 10/2011; 1(1).