Publications (2)2.33 Total impact
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ABSTRACT: In this study, 5-Flurouracil (5-FU) induced toxic effects were evaluated after treating with morin with respect to biochemical and morphological evaluation of erythrocyte with reference to clinical blood parameters, membrane associated damages, lipid peroxidation, ATPases and antioxidant enzymes in in vitro erythrocyte after scheduled treatment with 5-FU. 5-FU was prepared using saline followed by administration in rats to evaluate its toxic effects. Protective effect of morin was assessed by estimating antioxidant marker enzymes, lipid peroxidation, membrane bound enzymes and morphological changes, clinical hematological parameters by automated and manual standard protocols. 5-FU administration moderately increased the percentage of hemolysis, carboxyhemoglobin, hemin, lipid peroxidation and osmotic fragility in rat erythrocytes whereas morin treatment significantly altered to near normalcy. The levels of protein carbonyls significantly increased whereas the level of protein thiol decreased significantly in erythrocytes treated with 5-FU and reverted back to near normal upon morin treatment. The level of antioxidant enzyme and membrane bound ATPases were decreased significantly in 5-FU treated erythrocytes and brought back to the normal levels. The present study signifies that morin treatment can reduce the abnormalities posed by 5-FU by ameliorating oxidative stress. These clinical and biochemical observations shed the possibility that morin may render protection against 5-FU treated toxicity.Biomedicine & Preventive Nutrition. 01/2013; 3(1):19–25.
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ABSTRACT: In general, oxidative stress resulting from an imbalance between prooxidant and antioxidant systems plays an important role in the pathogenesis of cancer. Morin (3,5,7,2',4'-pentahydroxyflavone), a member of the flavanol group, has been shown to possess chemopreventive potential against hepatocellular and colon cancer in experimental animals. Given the demonstrated importance of morin, aim of the present study was to evaluate the effect of morin on antiproliferative and anticarcinogenic effect against DMBA-induced experimental mammary carcinogenesis. Oral administration of 7,12-dimethylbenz(a)-anthracene (25 mg/kg body weight) to rats resulted in significant reduction of body weight, enzymic antioxidants (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase), and nonenzymic antioxidants (reduced glutathione, vitamin C, and vitamin E). The levels of lipid peroxidation markers (thiobarbituric acid reactive substances and hydroperoxides) and tumor markers such as CA 15-3, AFP and CEA in serum were increased significantly in cancer-induced animals as compared to control rats. Oral supplementation of morin at a dose of 50 mg/kg body weight significantly improved the body weight, enzymic, and nonenzymic antioxidants and considerably decreased the lipid peroxidation marker and tumor markers levels. Histological observations also correlated with the biochemical parameters. Tumor bearing animals showed marked increase in proliferating cell nuclear antigen-positive cells and also the number of AgNOR/nuclei compared with control rats while this expression levels were significantly reduced upon morin treatment. Thus, this study reveals the possible beneficial effect of morin as chemopreventive agent against the oxidative stress induced during mammary carcinogenesis.Molecular and Cellular Biochemistry 02/2012; 364(1-2):79-92. · 2.33 Impact Factor
University of Madras
Chennai, Tamil Nādu, India
- Department of Biochemistry