Liang Wang

Jiangsu University, Chenkiang, Jiangsu, China

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Publications (7)15.11 Total impact

  • Lin Mei · Liang Wang · Qianqian Li · Jiangnan Yu · Ximing Xu ·
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    ABSTRACT: Background: In this study the effects of processing conditions of acid degumming and enzymatic degumming on the removal of phospholipids from Silybum marianum seed oil were investigated and the degumming efficiency was compared based on the phospholipid content. Results: An orthogonal array experimental design was performed to optimise the process of citric acid degumming. Based on range analysis and analysis of variance, the optimal processing conditions were determined to be a citric acid dosage of 3 g kg(-1) , a degumming temperature of 70 °C, a water addition of 40 mL kg(-1) and a degumming time of 30 min. Under these conditions the phospholipid content of degummed S. marianum seed oil was reduced from 273.0 to 128.1 mg kg(-1) . In the case of enzymatic degumming, the effects of enzyme reaction time and enzyme dosage were investigated using single-factor experiments. The optimal processing conditions were found to be an enzyme reaction time of 6 h and an enzyme dosage of 100 mg kg(-1) oil. Under these conditions the phospholipid content of degummed S. marianum seed oil was reduced to 17.95 mg kg(-1) . Conclusion: The results indicated that enzymatic degumming is more effective than acid degumming.
    Journal of the Science of Food and Agriculture 08/2013; 93(11). DOI:10.1002/jsfa.6109 · 1.71 Impact Factor
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    ABSTRACT: A continuous conversion process of rice starch hydrolysate to 2-keto-D-gluconic acid (2KGA) by Arthrobacter globiformis C224 was developed. Its feasibility for industrial application was also evaluated. Results showed that the initial cell concentration exceeding 1.25 g/L met the continuous 2KGA production at a stable dilution rate and media composition, while the dilution rate and feeding glucose concentration had a significant effect on 2KGA production performance. The optimal operating parameters were obtained as: 0.090/h of dilution rate and 171.0 g/L of feeding glucose concentration. Under these conditions, the steady state had a produced 2KGA concentration of 124.74 g/L, average volumetric productivity of 11.23 g/L/h, and yield of 0.97 g/g. In conclusion, continuous 2KGA production by the A. globiformis C224 strain would be a superior industrial process for the production of 2KGA in terms of its high 2KGA productivity and yield.
    Biotechnology and Bioprocess Engineering 08/2013; 18(4):709-714. DOI:10.1007/s12257-012-0831-6 · 1.11 Impact Factor
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    ABSTRACT: Interest in glycerol dehydratase (GDHt) has increased beyond academic circles in the past few decades because of its role in the fermentation pathway for producing industrial 1,3- propanediol (1,3-PD). This paper introduces the research progress of GDHt with special emphases on its property and structure, gene cloning, mechanism of inactivation and reactivation as well as irrational design and rational design
    01/2013; 634-638(1):892-895. DOI:10.4028/
  • Li-Na Ju · Shan-Shan Tong · Liang Wang · Jiang-Nan Yu · Xi-Ming Xu ·
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    ABSTRACT: As many traditional Chinese medicines have been founded to have protective effect on liver damage in recent years, they have also got involved in increasingly wide clinical application. Meanwhile, with the development of new hepatic protective formulations of traditional Chinese medicines, we have set increasingly higher requirements for quality control methods and measures. This essay summarizes the advance in studies on hepatic protective formulations of traditional Chinese medicine and their quality control methods in the combination of relevant domestic and foreign literatures, looking into the future of the development of new hepatic protective formulations of traditional Chinese medicines.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 10/2012; 37(20):3007-11.
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    ABSTRACT: 1,3-Propanediol (1,3-PD), an important material for chemical industry, is biologically synthesized by glycerol dehydratase (GDHt) and 1,3-propanediol dehydrogenase (PDOR). In present study, the dhaBCE and dhaT genes encoding glycerol dehydratase and 1,3-propanediol dehydrogenase respectively were cloned from Citrobacter freundii and co-expressed in E. coli. Sequence analysis revealed that the cloned genes were 85 and 77 % identical to corresponding gene of C. freundii DSM 30040 (GenBank No. U09771), respectively. The over-expressed recombinant enzymes were purified by nickel-chelate chromatography combined with gel filtration, and recombinant GDHt and PDOR were characterized by activity assay, kinetic analysis, pH, and temperature optimization. This research may form a basis for the future work on biological synthesis of 1,3-PD.
    Molecular Biotechnology 07/2012; 54(2). DOI:10.1007/s12033-012-9585-9 · 1.88 Impact Factor
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    ABSTRACT: The purpose of this study was to develop porous silica nanoparticles (PSNs) as a carrier to improve oral bioavailability of poorly water-soluble drugs, using silymarin as a model. PSNs were synthesized by reverse microemulsion and ultrasonic corrosion methods. A 3-day release formulation consisting of a silymarin solid dispersion, a hydrophilic gel matrix and silymarin-loaded PSNs was prepared. In vitro release studies indicated that both the silymarin-loaded PSNs and the 3-day release formulation showed a typical sustained-release pattern over a long period, about 72 h. The in vivo studies revealed that the 3-day release formulation gave a significantly higher plasma concentration and larger area under the concentration-time curves than commercial tablets when orally administered to beagle dogs. This implies that the prepared 3-day release formulation significantly enhanced the oral bioavailability of silymarin, suggesting that PSNs can be used as promising drug carriers for oral sustained release systems. Thus providing a technically feasible approach for improving the oral bioavailability and long-term efficacy of poorly soluble drugs.
    Acta biomaterialia 02/2012; 8(6):2104-12. DOI:10.1016/j.actbio.2012.02.011 · 6.03 Impact Factor
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    ABSTRACT: The purpose of this study was to develop a sustained drug-release model for water-soluble drugs using silica nanoparticles. Hollow-type mesoporous silica nanoparticles (HMSNs) were prepared using Na(2)CO(3) solution as the dissolution medium for the first time. The water-soluble compound, silybin meglumine, was used as the model drug. The Wagner-Nelson method was used to calculate the in vivo absorption fraction. The results of transmission electron microscopy and nitrogen adsorption revealed that the empty HMSNs had uniformly distributed particles of size 50-100 nm, a spherical appearance, a large specific surface area (385.89 ± 1.12 m(2)/g), and ultralow mean pore size (2.74 nm). The highly porous structure allowed a large drug-loading rate (58.91% ± 0.39%). In 0.08 M Na(2)CO(3) solution, silybin meglumine-loaded HMSNs could achieve highly efficacious and long-term sustained release for 72 hours in vitro. The results of in vitro-in vivo correlation revealed that HMSNs in 0.08 M Na(2)CO(3) solution had a correlation coefficient R(2) value of 0.9931, while those of artificial gastric juice and artificial intestinal juice were only 0.9287 and 0.7689, respectively. The findings of in vitro-in vivo correlation indicate that HMSNs together with Na(2)CO(3) solution could achieve an excellent linear relationship between in vitro dissolution and in vivo absorption for 72 hours, leading to a promising model for sustained release of water-soluble drugs.
    International Journal of Nanomedicine 02/2012; 7:753-62. DOI:10.2147/IJN.S28348 · 4.38 Impact Factor