Daniel Ciepielewski

Publications (3)19.12 Total impact

• Source

  Available from: Jerome Extermann

  Dataset: Harmonic nanocrystals for biolabeling: a survey of optical properties and biocompatibility

  Davide Staedler, Thibaud Magouroux, Rachid Hadji, Cécile Joulaud, Jérôme Extermann, Sebastian Schwung, Solène Passemard, Christelle Kasparian, Gareth Clarke, Mathias Gerrmann, Ronan Le Dantec, Yannick Mugnier, Daniel Rytz, Daniel Ciepielewski, Christine Galez, Sandrine Gerber-Lemaire, Lucienne Juillerat-Jeanneret, Luigi Bonacina, Jean-Pierre Wolf
• Source

  Available from: Jerome Extermann

  Article: High-speed tracking of murine cardiac stem cells by harmonic nanodoublers.

  Thibaud Magouroux, Jerome Extermann, Pernilla Hoffmann, Yannick Mugnier, Ronan Le Dantec, Marisa E Jaconi, Christelle Kasparian, Daniel Ciepielewski, Luigi Bonacina, Jean-Pierre Wolf
  [show abstract] [hide abstract]
  ABSTRACT: Potassium niobate nonlinear nanoparticles are used for the first time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo-bleaching and unlimited excitation wavelength flexibility. The potential of this approach is made evident for tissue-regeneration studies and applications, by capturing a high-speed movie of ESC-derived cardiomyocytes autonomously beating within a cluster. Time-resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level.
  Small 08/2012; 8(17):2752-6. · 8.35 Impact Factor
• Source

  Available from: Jerome Extermann

  Article: Harmonic nanocrystals for biolabeling: a survey of optical properties and biocompatibility.

  Davide Staedler, Thibaud Magouroux, Rachid Hadji, Cécile Joulaud, Jérôme Extermann, Sebastian Schwung, Solène Passemard, Christelle Kasparian, Gareth Clarke, Mathias Gerrmann, Ronan Le Dantec, Yannick Mugnier, Daniel Rytz, Daniel Ciepielewski, Christine Galez, Sandrine Gerber-Lemaire, Lucienne Juillerat-Jeanneret, Luigi Bonacina, Jean-Pierre Wolf
  [show abstract] [hide abstract]
  ABSTRACT: Nonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multiphoton microscopy. We present for the first time a complete survey of the properties of five nanomaterials (KNbO(3), LiNbO(3), BaTiO(3), KTP, and ZnO), describing their preparation and stabilization and providing quantitative estimations of their nonlinear optical response. In the light of their prospective use as biological and clinical markers, we assess their biocompatibility on human healthy and cancerous cell lines. Finally, we demonstrate the great potential for cell imaging of these inherently nonlinear probes in terms of optical contrast, wavelength flexibility, and signal photostability.
  ACS Nano 02/2012; 6(3):2542-9. · 10.77 Impact Factor