Publications (2)2.82 Total impact
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Article: [The purification and characterization of polysaccharides isolated from Ginkgo biloba and their in vitro antioxidant activities].
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ABSTRACT: To isolate and identify the polysaccharides of Ginkgo biloba and determine their antioxidant activities in vitro. Used hot extraction and alcohol precipitation to get the crude polysaccharides, removed protein with Sevag method, further purify by column chromatography packed with DEAE-52 and DEAE-Sepharose Fast Flow. The homogeneity and molecular weights were evaluated, configuration and monosaccharide composition were measured by IR and high performance ion chromatography analysis, the scavenging activities of GBPB-S on hydroxyl radical and DPPH were measured. The molecular weights of GBPB-W and GBPB-S were 26 300 and 19 100, and both of them were composed of rhamnose, arabinose, galactose, glucose and mannose with the ratios of (3.48: 8.47:3.73: 1.76: 1) and (5.34: 5.37: 5.27: 1:1.68). GBPB-S had certain scavenging effect on hydroxyl radical and DPPH. The polysaccharide isolated from Ginkgo biloba has a direct antioxidant activities in vitro.Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 12/2011; 34(12):1950-3. -
Article: Structure characterization and antioxidant activity of a novel polysaccharide isolated from pulp tissues of Litchi chinensis.
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ABSTRACT: A novel polysaccharide (LCP50S-2) with antioxidant activity was isolated from Litchi chinensis Sonn. The structure of LCP50S-2 was elucidated on the basis of physicochemical and instrumental analyses, and its average molecular weight was determined by gel permeation chromatography to be 2.19 × 10(2) kDa. The backbone of LCP50S-2 was composed of (1→3)-linked β-L-rhamnopyranosyl residues, (1→4)-linked α-D-xylopyranosyl residues, (1→4)-linked β-D-glucopyranosyl residues, and (1→4)-linked α-D-glucopyranosyl residues which branched at O-6. The two branches consisted of α-L-arabinopyranosyl residues and (1→6)-linked β-D-galactopyranosyl residues terminated with α-L-arabinopyranosyl residues, respectively. In the in vitro antioxidant assay, LCP50S-2 was found to possess DPPH radical-scavenging activity and hydroxyl radical-scavenging activity with IC(50) values of 220 and 266 μg/mL, respectively.Journal of Agricultural and Food Chemistry 11/2011; 59(21):11548-52. · 2.82 Impact Factor
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2011
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Jinan University (Guangzhou, China)
Guangzhou, Guangdong Sheng, China
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