B. Anupama

Osmania University, Bhaganagar, Andhra Pradesh, India

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Publications (7)10.9 Total impact

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    ABSTRACT: Mixed-ligand Cu(II), Co(II) complexes of formulae [Co(NSALT)(A.A)(H2O)](1), [Co(OHAPT)(A.A)H2O](2), and [Cu(ESALT)(ABPH)H2O] (3) were obtained by refluxing methanol solutions of copper, cobalt chlorides with the appropriate ligands. The complexes were characterized by the ESI-MASS, vibrational spectroscopy (Fourier transform-IR), (1)H-NMR spectroscopy, UV-vis spectroscopy, TGA, ESR, SEM and powder XRD. The preliminary DNA-binding activity of the complexes was studied by recording electronic absorption spectra of the complexes in presence of CT-DNA. The binding constants of three complexes towards calf thymus DNA (CT-DNA) [1.2 × 10(4) M(-1) for 1, 2.5 × 10(4) M(-1) for 2, and 3.0 × 10(4) M(-1) for 3] indicate strong interaction of 3. Changes in the fluorescence of ethidium bromide in the presence of DNA suggest intercalation into or electrostatic interactions with CT DNA. The quenching constants, KSV towards-DNA calculated through fluorescence spectra are 2.9 × 10(4) M(-1)for 1, 1.8 × 10(4) M(-1) for 2, and 3.2 × 10(4) M(-1) for 3. Docking studies on DNA complexes confirm the binding of 1 and 2 in the major groove of CT-DNA (CTP-1 Endonuclease). Moreover, the antibacterial effect of 1-3 against the five bacterial species was evaluated. The metal complexes have cleavage affinity towards PBR322 plasmid. Furthermore, the antioxidant activities of the complexes were determined by DPPH scavenging activity method.
    Journal of Fluorescence 09/2014; 25(1). DOI:10.1007/s10895-014-1456-2 · 1.93 Impact Factor
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    ABSTRACT: A series of transition metal complexes of Co(II), Ni(II), Zn(II), Fe(III) and VO(IV) have been synthesized involving the Schiff base, 2,3-dimethyl-1-phenyl-4-(2-hydroxy-3-methoxy benzylideneamino)-pyrazol-5-one(L), obtained by condensation of 4-aminoantipyrine with 3-methoxy salicylaldehyde. Structural features were obtained from their FT-IR, UV-vis, NMR, ESI Mass, elemental analysis, magnetic moments, molar conductivity and thermal analysis studies. The Schiff base acts as a monovalent bidentate ligand, coordinating through the azomethine nitrogen and phenolic oxygen atom. Based on elemental and spectral studies six coordinated geometry is assigned to Co(II), Ni(II), Fe(III) and VO(IV) complexes and four coordinated geometry is assigned to Zn(II) complex. The interaction of metal complexes with Calf thymus DNA were carried out by UV-VIS titrations, fluorescence spectroscopy and viscosity measurements. The binding constants (Kb) of the complexes were determined as 5 × 10(5) M(-1) for Co(II) complex, 1.33 × 10(4) M(-1) for Ni(II) complex, 3.33 × 10(5) M(-1) for Zn(II) complex, 1.25 × 10(5) M(-1) for Fe(III) complex and 8 × 10(5) M(-1) for VO(IV) complex. Quenching studies of the complexes indicate that these complexes strongly bind to DNA. Viscosity measurements indicate the binding mode of complexes with CT DNA by intercalation through groove. The ligand and it's metal complexes were screened for their antimicrobial activity against bacteria. The results showed the metal complexes to be biologically active, while the ligand to be inactive.
    Journal of Fluorescence 04/2014; 24(4). DOI:10.1007/s10895-014-1386-z · 1.93 Impact Factor
  • Source
    M. Sunita · B. Anupama · B. Umesh · C. Gyana Kumari ·
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    ABSTRACT: New two copper complexes of type [Cu(Bzimpy)(L)H2O]SO4 (where L= 2,2’ bipyridine (bpy), and ethylene diamine (en)), Bzimpy =2,6-bis(benzimidazole-2yl)pyridine have been synthesized and characterized by elemental analyses, molar conductance measurements, magnetic susceptibility measurements, mass, IR, electronic and EPR spectral studies. Based on elemental and spectral studies six coordinated geometries were assigned to the two complexes. DNA-binding properties of these metal complexes were investigated using absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and thermal denaturation methods. Experimental studies suggest that the complexes bind to DNA through intercalation. These complexes also promote the cleavage of plasmid pBR 322, in the presence of H2O2.
    Arabian Journal of Chemistry 02/2014; DOI:10.1016/j.arabjc.2014.01.017 · 3.73 Impact Factor
  • B. Anupama B. Anupama · Ch. Venkata Ramana Reddy · C. Gyana Kumari ·

    04/2013; 2(2):461-466. DOI:10.7598/cst2013.399
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    Padmaja Mendu · J. Pragathi · B. Anupama · C. Gyana Kumari ·
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    ABSTRACT: A series of Cu(II), Ni(II), Co(II), Mn(II), and Zn(II) complexes have been synthesized from the schiff base ligand L. The schiff base ligand [(4-oxo-4H-chromen-3-yl) methylene] benzohydrazide (L) has been synthesized by the reaction between chromone-3-carbaldehyde and benzoyl hydrazine. The nature of bonding and geometry of the transition metal complexes as well as schiff base ligand L have been deduced from elemental analysis, FT-IR, UV-Vis, 1HNMR, ESR spectral studies, mass, thermal (TGA and DTA) analysis, magnetic susceptibility, and molar conductance measurements. Cu(II), Ni(II), Co(II), and Mn(II) metal ions are forming 1:2 (M:L) complexes, Zn(II) is forming 1:1 (M:L) complex. Based on elemental, conductance and spectral studies, six-coordinated geometry was assigned for Cu(II), Ni(II), Co(II), Mn(II), and Zn(II) complexes. The complexes are 1:2 electrolytes in DMSO except zinc complex, which is neutral in DMSO. The ligand L acts as tridentate and coordinates through nitrogen atom of azomethine group, oxygen atom of keto group of γ-pyrone ring and oxygen atom of hydrazoic group of benzoyl hydrazine. The 3D molecular modeling and energies of all the compounds are furnished. The biological activity of the ligand and its complexes have been studied on the four bacteria E. coli, Edwardella, Pseudomonas, and B. subtilis and two fungi pencillium and tricoderma by well disc and fusion method and found that the metal chelates are more active than the free schiff base ligand.
    E-Journal of Chemistry 04/2012; 9(4). DOI:10.1155/2012/839789 · 0.70 Impact Factor
  • M Sunita · M Padmaja · B Anupama · C Gyana Kumari ·
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    ABSTRACT: Four novel copper(II) complexes of the composition [CuLX] where L = 2,6-bis(benzimidazole-2yl)pyridine, X = dipyridophenazine (L1), 1,10-phenanthroline (L2), hydroxyproline (L3) and 2,6-pyridine dicarboxylic acid (L4) were synthesized and characterized by using elemental analysis, FT-IR, UV–vis, ESI-MS, molar conductance and magnetic susceptibility measurements. The complexes [CuLL1](NO3)2 [1], [CuLL2](NO3)2 [2], [CuLL3](NO3) [3] and [CuLL4] (NO3) [4] are stable at room temperature. In DMSO the complexes [1] and [2] are 1:2 electrolytes, [3] and [4] are 1:1 electrolytes. Based on elemental and spectral studies five coordinated geometry is assigned to all the four complexes. The interaction of four copper ion complexes with calf thymus DNA were carried out by UV-vis titrations, fluorescence spectroscopy, thermal melting and viscosity measurements .The binding constant (K(b)) of the above four metal complexes were determined as 5.43 × 10(4) M(-1), 2.56 × 10(4) M(-1), 1.21 × 10(4) M(-1) and 1.57 × 10(4) M(-1) respectively. Quenching studies of the four complexes indicates that these complexes strongly bind to DNA, out of all complex 1 is binding more strongly. Viscosity measurements indicate the binding mode of complexes with CT DNA by intercalation through groove. Thermal melting studies also support intercalative binding. The nuclease activity of the above metal complexes shows that 1, 2 and 3 complexes cleave DNA through redox chemistry.
    Journal of Fluorescence 02/2012; 22(3):1003-12. DOI:10.1007/s10895-012-1038-0 · 1.93 Impact Factor
  • Source
    B. Anupama · M. Padmaja · C. Gyana Kumari ·
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    ABSTRACT: A new series of transition metal complexes of Cu(II),Ni(II),Co(II), Zn(II) and VO(IV) have been synthesized from the Schiff base ligand (L) derived from 4-amino antipyrine and 5- bromo salicylaldehyde. The structural features of Schiff base and metal complexes were determined from their elemental analyses, thermogravimetric studies, magneticsusceptibility, molar conductivity, ESI-Mass, IR, UV-VIS, 1H NMR and ESR spectral studies. The data show that the complexes have composition of ML2 type. The UV-VIS, magnetic susceptibility and ESR spectral data suggest an octahedral geometry around the central metal ion. Biological screening of the complexes reveals that the Schiff base transition metal complexes show significant activity against microorganisms. Binding of Co(II) complex with calf thymus DNA (CT DNA) was studied by spectral methods.
    E-Journal of Chemistry 01/2012; 9(1). DOI:10.1155/2012/291850 · 0.70 Impact Factor