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ABSTRACT: Antioxidant activity of 70% acetone extracts of raw and processed seeds of Jack bean (Canavalia ensiformis L. DC) was evaluated by various in vitro antioxidant assays, including total antioxidant, free radical scavenging, reducing
power, metal ion chelating, β-carotene/linoleic acid bleaching, and antihemolytic activities. The total phenolics and tannin
contents were higher in the extract of seeds processed by autoclaving with 1% ash solution (3.2 and 1.6 g/100 g extract, respectively).
In general, all the extracts of processed seeds exhibited higher activity in various antioxidant systems, when compared to
raw seeds but significant differences were noticed between processing methods. The extract of seeds autoclaved with 1% sugar
solution showed higher DPPH radical scavenging activity (IC50 10.6 mg/mL). Interestingly, the extract of dry heated seeds registered higher inhibition of hemolysis (76.1%) compared to
standards butylated hydroxyanisole (BHA) (66.2%) and α-tocopherol (59.3%) at the concentration of 500 μg/mL.
Key words
Canavalia ensiformis
–total phenolics–legume–antioxidant activity–antihemolytic
Food science and biotechnology 04/2012; 20(3):585-591. · 0.49 Impact Factor
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ABSTRACT: In the present study, antioxidant activities of the phenolic extracts from H. isora fruits and C. pentandra seeds were investigated by employing established in vitro systems, which included reducing power, OH●, DPPH●, ABTS●+, linoleic acid emulsion, metal chelation and antihemolytic activity. The extracts of C. pentandra contained relatively higher levels of total phenolics and flavonoids than those of H. isora. All the extracts showed dose dependent reducing power activity and moreover, they were well correlated with the total phenolic
substances. A similar dose dependant trend has also been observed for hydroxyl radical scavenging activity and DPPH● radical scavenging activity. Further, addition of 250μg of extracts to the reaction mixture produced 41.3–54.6% peroxidation
inhibiting activity during 60h of incubation. The potential of multiple antioxidant activity of samples can be further evidenced
by inhibition of reactive oxygen mediated erythrocyte cell lysis and metal ion chelating activity.
Keywords
C. pentandra seeds–
H. isora fruits–Antioxidant activity–ABTS•+
–Phenolics and flavonoids
Journal of Food Science and Technology -Mysore- 04/2012; · 0.50 Impact Factor
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ABSTRACT: In the present study, chloroform, acetone, and methanol extracts of Solanum torvum and Solanum nigrum leaves and fruit were evaluated for their in vitro antioxidant activity using ferric reducing antioxidant power, 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ABTS·+, iron chelation, and antihemolytic activity. Among the various solvents used, the maximum yield was obtained in chloroform
followed by acetone and methanol. The total phenolics were also significantly higher in the chloroform extracts and the in vitro antioxidant activity was shown to be highly dependant on the total phenolic content (p<0.01). The DPPH· and 2,2′azinobis(3-ethylbenzothiozoline-6-sulfonic acid) diammonium salt (ABTS) cation radical scavenging activities were
well proved with the ferric reducing antioxidant capacity of the extracts. The potential of multiple antioxidant activity
of samples can be further evidenced by inhibition of reactive oxygen mediated erythrocyte cell lysis and metal ion chelating
activity. In conclusion, the results presented here implied leaves and fruits of S. torvum and S. nigrum could be considered as health supplements and nutraceuticals/functional foods.
Keywords
Solanum torvum
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Solanum nigrum
-2,2-diphenyl-1-picryl-hydrazyl (DPPH·)-2,2′azinobis(3-ethylbenzothiozoline-6-sulfonic acid) diammonium salt (ABTS)-antihemolytic activity
Food science and biotechnology 04/2012; 19(1):121-127. · 0.49 Impact Factor
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ABSTRACT: Antioxidant potential and total phenolics content of 70% acetone extracts of the raw and processed seeds of Bauhinia vahlii were evaluated. The extract of raw seeds contained higher levels of total phenolics (30.8 g/100 g) and tannins (19.6 g/100
g) compared to dry heated and soaking followed by autoclaving seed extracts. Extracts were screened for antioxidant and free
radical scavenging activities using various chemical and in vitro model systems. In all the models, except DPPH radical scavenging activity, the extract from raw seeds manifested the strongest
antioxidant activity than that from processed seeds. In β-carotene/linoleic acid emulsion system and superoxide scavenging
activity, the raw seed extract registered more activity when compared to the standards (butylated hydroxyanisole and α-tocopherol).
Whereas, the extract from dry heated seed exhibited higher DPPH· scavenging activity (IC50 70.77 μg/mL) than the raw seeds (IC50 74.4 μg/mL). This study has to some extent validated the antioxidant potential of the seeds of B. vahlii.
Keywords
Bauhinia vahlii
-polyphenol-antioxidant activity-β-carotene-2,2-diphenylpicryl-1-picryl-hydrazyl (DPPH·)
Food science and biotechnology 04/2012; 19(2):503-509. · 0.49 Impact Factor
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ABSTRACT: Ammannia baccifera L. has been reported as folklore remedy for the treatment of inflammation and tumor in the state of Rajasthan, India.
The present study was designed to investigate the antioxidant, anti-inflammatory and anti-nociceptive effects of the methanol extract from the aerial parts of Ammannia baccifera under in vitro and in vivo models.
The in vitro antioxidant activity of the extract was measured using DPPH, superoxide, hydroxyl and nitric oxide radicals. The anti-inflammatory activity was evaluated using carrageenan induced paw edema. Analgesic activity of the methanol extract was estimated against acetic acid-induced writhing and hot plate methods.
The IC(50) value for free radical scavenging activity of this extract was significantly superior over the positive standards butylated hydroxyl anisole (BHA) and rutin. The extract exhibited significant anti-inflammatory and analgesic activities at the dose of 100 and 200mg/kg p.o. The analgesic effect of the higher dose of the extract (200mg/kg) was comparable with the standard drugs aspirin and morphine.
The present study scientifically validated the traditional use of this plant against inflammation.
Journal of ethnopharmacology 03/2012; 140(2):230-3. · 2.32 Impact Factor
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ABSTRACT: Total phenolics and the antioxidative properties of two varieties of horse gram (Macrotyloma uniflorum) were studied. The raw and dry-heated seed samples were extracted successively with methanol and 70% acetone separately. After removing the solvents, the extracts were freeze-dried. The black seeds contained relatively high levels of total phenolics and tannins than the brown seeds with respect to the treatments and solvents extraction. The extracts were subjected to assess their potential antioxidant activities using systems such as α,α-diphenyl-β-picrylhydrazyl (DPPH), ABTS+, FRAP, linoleic acid emulsion, and OH. The superoxide anion radical-scavenging activity was found to be higher in 70% acetone extract of the both raw and dry heated seeds of the respective varieties at the concentration of 600 μg in the reaction mixture. The DPPH radical and ABTS cation radical-scavenging activities were well proved and related with the ferric-reducing/antioxidant capacity of the extracts. Interestingly, among the various extracts, 70% acetone extracts of dry-heated samples of brown variety, and raw and dry-heated samples of black variety showed significantly (P < 0.05) higher hydroxyl radical-scavenging activity. In general, all extracts exhibited good antioxidant activity (53.3–73.1%) against the linoleic acid emulsion system but were significantly (P < 0.05) lower than the synthetic antioxidant, BHA (93.3%).
Food Chemistry.
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ABSTRACT: The stem bark and fruits of Ficus bengalensis L. and Ficus racemosa L. are used in India for the treatment of diabetes and a number of other diseases. Since these effects may be correlated with the presence of antioxidant compounds, methanol and 70% acetone (acetone:water, 70:30) extracts of F. bengalensis (aerial root) and F. racemosa (stem bark) were evaluated for their antioxidant activity and radical scavenging capacity in comparison with Camellia sinensis (L.) O. Kuntz (green tea). Methanol extracts of green tea and F. bengalensis and 70% acetone extract of F. racemosa contained relatively higher levels of total phenolics than the other extracts. The antioxidant potential of the extracts were assessed by employing different in vitro assays such as reducing power assay, DPPH, ABTS+ and OH radical scavenging capacities, peroxidation inhibiting activity through linoleic acid emulsion system, antihemolytic assay by hydrogen peroxide induced method and metal ion chelating ability. Though all the extracts exhibited dose dependent reducing power activity, methanol extracts of all the samples were found to have more hydrogen donating ability. Similar line of dose dependent activity has been maintained in all the samples in DPPH and OH scavenging systems. All the extracts exhibited antioxidant activity against the linoleic acid emulsion system (34–38%). The potential of multiple antioxidant activity was evident as it possessed antihemolytic activity and metal ion chelating potency.
Food Chemistry.
