Publications (2)3.34 Total impact

  • X H Gu · Y Hao · X L Wang
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    ABSTRACT: Oxidative stress injury is one important factor in intestinal mucosal barrier damage. Expression of heat shock protein (HSP)70 is an endogenous mechanism by which living cells adapt to stress. This study was undertaken to investigate the protective effects of HSP70 on intestinal oxidative stress. Two hundred and forty broilers were injected intraperitoneally with HSP70 inducer l-(1)-glutamine or with the inhibitor quercetin. Twenty-four hours later, they were heat stressed for 0, 2, 3, 5, and 10 h, respectively, at 36 ± 1°C. The l-(1)-glutamine significantly increased HSP70 expression (P < 0.001). At 2 h or 3 h of heat stress, the HSP70 expression obviously elevated (P < 0.001). Levels of corticosterone and the heterophil:lymphocyte ratio significantly increased when HSP70 expression was inhibited (P < 0.0001). Serum corticosterone was negatively correlated with the HSP70 expression at 3 h of heat stress (P = 0.0015; R = -0.6537). Heat shock protein 70 significantly protected the integrity of the intestinal mucosa from heat stress, with significantly decreased lactic dehydrogenase when HSP70 expression was enhanced (P < 0.001). In addition, heat-stress time significantly affected the lactic dehydrogenase release (P < 0.001). Furthermore, HSP70 significantly elevated antioxidant enzyme activities (such as superoxide dismutase, glutathione peroxidase, and total antioxidant capacity) and inhibited lipid peroxidation to relieve intestinal mucosal oxidative injury (P < 0.001). These results suggest that HSP70 is capable of protecting the intestinal mucosa from heat-stress injury by improving antioxidant capacity of broilers and inhibiting the lipid peroxidation production.
    Poultry Science 04/2012; 91(4):790-9. DOI:10.3382/ps.2011-01628 · 1.67 Impact Factor
  • Y Hao · X H Gu · X L Wang
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    ABSTRACT: The objective of this study was to investigate the relationship between heat shock protein 70 (HSP70) overexpression and intestinal structure and digestive function in heat-stressed broilers. In total, 240 male broilers were injected intraperitoneally with l-(1)-glutamine (0.75 mg/kg of BW) or quercetin (5 mg/kg of BW). Twenty-four hours later, they were heat-stressed for 0, 2, 3, 5, and 10 h, respectively, under 36 ± 1°C. The HSP70 protein and mRNA expression were obviously elevated at 3 h of heat stress, and glutamine induced the overexpression of HSP70 in the jejunal mucosa at different heat-stress times (P < 0.01). No significant change of jejunal villus height, crypt, and villus height:crypt ratio were observed after heat stress, and there were no effects of HSP70 overexpression on intestinal morphology under heat stress. The overexpression of HSP70 significantly increased alkaline phosphatase activity at 3 h of heat stress (P < 0.01). There was a strong correlation between HSP70 expression and the digestive enzyme activity (P ≤ 0.001). The overexpression of HSP70 significantly increased the amylase, lipase, and trypsin activity under heat stress (P < 0.001). These results demonstrated that glutamine was a good HSP70 enhancer to establish an HSP70 overexpression model. Although the overexpression of HSP70 did not change intestinal morphology conditions, it significantly increased broiler digestive enzyme activity under heat stress.
    Poultry Science 04/2012; 91(4):781-9. DOI:10.3382/ps.2011-01627 · 1.67 Impact Factor