Przemyslaw J Porebski, Maria Klimecka,
Maksymilian Chruszcz,
Robert A Nicholls,
Krzysztof Murzyn,
Marianne E Cuff,
Xiaohui Xu,
Marcin Cymborowski,
Garib N Murshudov,
Alexei Savchenko,
Aled Edwards,
Wladek Minor
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ABSTRACT: Dethiobiotin synthetase (DTBS) is involved in the biosynthesis of biotin in bacteria, fungi, and plants. As humans lack this pathway, DTBS is a promising antimicrobial drug target. We determined structures of DTBS from Helicobacter pylori (hpDTBS) bound with cofactors and a substrate analog, and described its unique characteristics relative to other DTBS proteins. Comparison with bacterial DTBS orthologs revealed considerable structural differences in nucleotide recognition. The C-terminal region of DTBS proteins, which contains two nucleotide-recognition motifs, differs greatly among DTBS proteins from different species. The structure of hpDTBS revealed that this protein is unique and does not contain a C-terminal region containing one of the motifs. The single nucleotide-binding motif in hpDTBS is similar to its counterpart in GTPases; however, isothermal titration calorimetry binding studies showed that hpDTBS has a strong preference for ATP. The structural determinants of ATP specificity were assessed with X-ray crystallographic studies of hpDTBS·ATP and hpDTBS·GTP complexes. The unique mode of nucleotide recognition in hpDTBS makes this protein a good target for H. pylori-specific inhibitors of the biotin synthesis pathway. Database Coordinates and structure factors for structures presented in this article have been deposited in the Protein Data Bank under the following accession codes: 2QMO - apo-form; 3MLE - hpDTBS·8-ac·ADP· in P2(1) 2(1) 2(1) form; 3QXC - hpDTBS·ATP; 3QXH - hpDTBS·8-ac:ADP· in C2 form; 3QXJ - hpDTBS·GTP; 3QXS - hpDTBS·ANP; 3QXX - 8-ac·GDP·; and 3QY0 - hpDTBS·GDP·. Structured digital abstract • hpDTBS and hpDTBS bind by x-ray crystallography (View interaction).
FEBS Journal 01/2012; · 3.79 Impact Factor
