Boonsong Ongphiphadhanakul

Ramathibodi Hospital, Krung Thep, Bangkok, Thailand

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Publications (103)224.13 Total impact

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    ABSTRACT: Objective: Vitamin D deficiency is related to increased risks of a number of diseases. To date, at least three candidate genes, i.e. vitamin D binding protein gene (GC), CYP2R1 and DHCR7/NADSYN1, have been associated with serum 25-hydroxyvitamin D (25(OH)D), but their influence on the prevalence of vitamin D deficiency in relation to other known risk factors has not been clearly defined.Methods: Subjects consisted of 4,476 individuals aged 14-93 years from the Thailand 4th National Health Examination Survey (2008-2009) and the Electricity Generating Authority of Thailand (EGAT) (2008) cohorts. The GC rs2282679 polymorphism on chromosome 4q12-q13 was genotyped by real-time PCR. Serum 25(OH)D was measured by liquid chromatography/tandem mass spectrometry. Vitamin D deficiency was defined as a 25(OH)D concentration lower than 20 ng/mL.Results: Data were expressed as mean ± SD. There were 2,747 (61.4%) males and 1,729 (38.6%) females in the study, with an average BMI of 23.7 ± 4.2 kg/m2 and a mean total 25(OH)D of 28.9 ± 9.0 ng/mL. Serum 25(OH)D levels decreased progressively with the presence of the C allele. Using multiple logistic regression analysis, vitamin D deficiency was significantly associated with the GC rs2282679 genotype (OR per C allele 1.80, 95% CI 1.57-2.01) independent of established risk factors for vitamin D deficiency including age, gender and body mass index.Conclusion: Vitamin D binding protein gene polymorphism is associated with lower 25(OH)D levels independent of age, gender and adiposity in Thais.
    11/2014;
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    C Sritara, A Thakkinstian, B Ongphiphadhanakul
    10/2014;
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    ABSTRACT: A number of healthy workers rarely exercise because of a lack of time or resources. Physical activity related to work and everyday travel may be more feasible, but evidence of its beneficial effect on bone health is scarce. We assessed if this form of physical activity was associated with higher bone mineral density (BMD) and stiffness index (SI) when adjusted for recreational physical activity, age, body mass index, smoking, alcohol consumption, education, and serum level of 25-hydroxyvitamin D. Healthy workers, aged 25-54 yr, of the Electricity Generating Authority of Thailand were surveyed. The outcomes were BMD (lumbar spine, femoral neck, and total hip) and calcaneal SI. Physical activity was estimated using the global physical activity questionnaire and considered active when >600 metabolic equivalent tasks (min). Of 2268 subjects, 74% were men. Active male subjects had significantly higher BMD at the femoral neck and total hip (p < 0.005). However, the association was not significant with male lumbar spine BMD, male SI, or any bone parameters in women (p > 0.05). In men, work and travel physical activity seems beneficial to male bone health; hence, it should be encouraged. Furthermore, smoking appeared harmful while moderate alcohol consumption was beneficial.
    Journal of Clinical Densitometry 05/2014; · 1.71 Impact Factor
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    ABSTRACT: Context Thyroid hormone is critical for fetal neurodevelopment. Perchlorate and thiocyanate decrease thyroidal iodine uptake by competitively inhibiting the sodium/iodide symporter. It is clear that perchlorate and thiocyanate anions can influence thyroid function. However, as pollutants in the environment their impact is conflicting. Objective The objective was to determine the effects of environmental perchlorate and/or thiocyanate exposure on thyroid function in first trimester pregnant women. Design and Patients A cross-sectional study was conducted in 200 pregnant Thai women with a gestational age ≤14 weeks. Measures Urinary iodide, perchlorate, thiocyanate and serum thyroid function tests were measured. Results The women were aged 28.6±6.1 years and the mean gestational age was 9.6±2.7 weeks. Median urinary iodide, perchlorate, and thiocyanate concentrations were 153.5 μ g/l, 1.9 μ g/l, and 510.5 μ g/l, respectively. Using Spearman's rank correlation analyses, there were positive correlations between serum TSH and urine perchlorate/creatinine (r 0.20, p=0.005); and TSH and thiocyanate/creatinine (r 0.22, p=0.001). There were negative correlations between FT4 and perchlorate/creatinine (r-0.18, p=0.01); and FT4 and thiocyanate/creatinine (r-0.19, p=0.008). In multivariate analyses adjusting for log thiocyanate/creatinine, log iodide/creatinine, and gestational age, log perchlorate/creatinine was positively associated with log TSH (p=0.002), and inversely associated with log FT4 (p= 0.002). Log thiocyanate/creatinine was a significant positive predictor of log TSH (p=0.02) in women with urine iodide <100 μ g/l. Conclusions Low-level environmental exposure to perchlorate and thiocyanate is common in Thailand. Low-level exposure to perchlorate is positively associated with TSH and negatively associated with FT4 in first trimester pregnant women using multivariate analyses. In multivariate analyses, thiocyanate exposure is also positively associated with TSH in a subgroup of pregnant women with low iodine excretion.
    The Journal of Clinical Endocrinology and Metabolism 04/2014; · 6.31 Impact Factor
  • Wichai Aekplakorn, La‐or Chailurkit, Boonsong Ongphiphadhanakul
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    ABSTRACT: Background Epidemiological studies of the association between exposure to bisphenol A (BPA) and diabetes have been inconsistent. The present study determined the levels of serum BPA in the Thai population and its association with hyperglycemia and diabetes.MethodsA total of 2,581 serum samples from the Thai National Health Examination Survey in 2009 were used to determine the levels of BPA. Impaired fasting glucose (IFG) was defined as fasting plasma glucose ≥100 and <126 mg/dL. Diabetes was defined by history of physician's diagnosis or fasting plasma glucose ≥126 mg/dL. Multinomial logistic regression was used to examine the association of serum BPA with IFG and diabetes.ResultsOf 2,581 samples tested, BPA was detected in a total of 2,135 (78.1%); among these, the overall geometric mean BPA was 0.34 ng/mL. Serum BPA level was significantly higher among those having diabetes or IFG compared with normoglycemia (0.52, 0.38 and 0.33 ng/mL, respectively, P <0.001). After adjusting for potential confounders, compared with the first quartile, the overall adjusted odds ratios of serum BPA concentration in the third and fourth quartiles for IFG were 1.72 (95% CI 1.19, 2.49) and 1.23 (95% CI 0.80, 1.90), respectively, and for diabetes 1.88 (95% CI 1.18, 2.99) and 1.83 (95% CI 1.12, 2.96), respectively, with a slightly stronger association among men than in women.Conclusions Serum BPA was not associated with IFG, but was positively associated with diabetes in the Thai population. Further prospective studies to confirm the relationship are needed.
    Journal of Diabetes 04/2014; · 2.94 Impact Factor
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    ABSTRACT: Using mediation analysis, a causal relationship between the AHSG gene and bone mineral density (BMD) through fetuin-A and body mass index (BMI) mediators was suggested. Fetuin-A, a multifunctional protein of hepatic origin, is associated with bone mineral density. It is unclear if this association is causal. This study aimed at clarification of this issue. A cross-sectional study was conducted among 1,741 healthy workers from the Electricity Generating Authority of Thailand (EGAT) cohort. The alpha-2-Heremans-Schmid glycoprotein (AHSG) rs2248690 gene was genotyped. Three mediation models were constructed using seemingly unrelated regression analysis. First, the ln[fetuin-A] group was regressed on the AHSG gene. Second, the BMI group was regressed on the AHSG gene and the ln[fetuin-A] group. Finally, the BMD model was constructed by fitting BMD on two mediators (ln[fetuin-A] and BMI) and the independent AHSG variable. All three analyses were adjusted for confounders. The prevalence of the minor T allele for the AHSG locus was 15.2 %. The AHSG locus was highly related to serum fetuin-A levels (P < 0.001). Multiple mediation analyses showed that AHSG was significantly associated with BMD through the ln[fetuin-A] and BMI pathway, with beta coefficients of 0.0060 (95 % CI 0.0038, 0.0083) and 0.0030 (95 % CI 0.0020, 0.0045) at the total hip and lumbar spine, respectively. About 27.3 and 26.0 % of total genetic effects on hip and spine BMD, respectively, were explained by the mediation effects of fetuin-A and BMI. Our study suggested evidence of a causal relationship between the AHSG gene and BMD through fetuin-A and BMI mediators.
    Osteoporosis International 02/2014; · 4.04 Impact Factor
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    ABSTRACT: A number of healthy workers rarely exercise because of a lack of time or resources. Physical activity related to work and everyday travel may be more feasible, but evidence of its beneficial effect on bone health is scarce. We assessed if this form of physical activity was associated with higher bone mineral density (BMD) and stiffness index (SI) when adjusted for recreational physical activity, age, body mass index, smoking, alcohol consumption, education, and serum level of 25-hydroxyvitamin D. Healthy workers, aged 25–54 yr, of the Electricity Generating Authority of Thailand were surveyed. The outcomes were BMD (lumbar spine, femoral neck, and total hip) and calcaneal SI. Physical activity was estimated using the global physical activity questionnaire and considered active when >600 metabolic equivalent tasks (min). Of 2268 subjects, 74% were men. Active male subjects had significantly higher BMD at the femoral neck and total hip (p < 0.005). However, the association was not significant with male lumbar spine BMD, male SI, or any bone parameters in women (p > 0.05). In men, work and travel physical activity seems beneficial to male bone health; hence, it should be encouraged. Furthermore, smoking appeared harmful while moderate alcohol consumption was beneficial.
    Journal of Clinical Densitometry 01/2014; · 1.71 Impact Factor
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    ABSTRACT: Objectives. Sclerostin, an osteocyte-specific protein, has been found to be related to adiposity and glucose metabolism. Irisin, a myokine, can affect browning of white fat and influence glucose and energy homeostasis. Taken together, this suggests a probable network among fat, bone, and muscle that may influence health outcomes. The aims of this study were to investigate the relationship of circulating sclerostin and irisin and their association with adiposity (assessed by body mass index (BMI)). Materials/Methods. A cross-sectional study included 98 adults with impaired fasting glucose and/or impaired glucose tolerance. 75 gm OGTT was performed in all subjects. Fasting plasma samples were obtained for glycated hemoglobin, calcium, creatinine, serum sclerostin and irisin. Results. Circulating irisin and sclerostin were highly correlated (r = -0.4; P < 0.001). After controlling for age, gender, and BMI, irisin was significantly related to sclerostin (P < 0.001). Multivariate linear regression analysis demonstrated that circulating sclerostin (β = -0.45; P < 0.05) and irisin (β = -0.46; P < 0.05) were negatively associated with BMI, independent of age in males. In females, no relationship of sclerostin or irisin to BMI was found. Conclusions. Circulating irisin and sclerostin are highly related. Interventions targeting irisin could affect sclerostin and vice versa.
    International Journal of Endocrinology 01/2014; 2014:261545. · 2.52 Impact Factor
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    ABSTRACT: Existing inconclusive data on the relationship between vitamin D status and human glucose homeostasis suggests that other factors, such as adiposity, might influence this relationship. The present study aimed to investigate the association between 25-hydroxyvitamin D [25(OH)D] and fasting plasma glucose (FPG) in the context of different amounts of total body fat in a healthy community-based population in Bangkok, Thailand. This cross-sectional study was a part of health survey of employees of the Electricity Generating Authority of Thailand. There were 1,990 healthy subjects (72.8% male) in this study. Total body fat was measured by bioelectrical impedance analysis. Total serum 25(OH)D, 25(OH)D3 and 25(OH)D2 were measured by LC-MS/MS. Age (r = 0.134, p < 0.001) and FPG (r = 0.089, p < 0.001) were positively correlated with 25(OH)D levels, while total body fat mass (r = -0.049,p = 0.03) were negatively correlated with 25(OH)D levels. 25(OH)D levels were higher in males than in females (65.0 +/- 0.5 vs. 53.5 +/- 0.5 nmol/L, p < 0.001). After controlling for age, gender and total fat mass, FPG was no longer correlated with 25(OH)D. However, when subjects were stratified according to fat-free mass tertiles and controlled for age and gender, there was a positive, although weak association between 25(OH)D levels and FPG (p = 0.01) in the lowest tertile. We therefore speculate that adiposity might influence the relationship of vitamin D status and FPG.
    BMC Endocrine Disorders 12/2013; 13(1):60. · 2.65 Impact Factor
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    ABSTRACT: Data on reference intervals of thyroid functions in Southeast Asia are limited. The aim of this study was to provide reference ranges of thyroid functions and thyroid autoantibodies in Thais. Serum samples from 2,545 apparently healthy non-pregnant subjects, aged >14 years, from the fourth Thai National Health Examination Survey were measured for TSH, FT4, antithyroperoxidase (TPO Ab), antithyroglobulin (Tg Ab), and antithyrotropin receptor antibodies (TRAb). A reference population was selected from the disease-free population by excluding those who had thyroid autoantibodies and TSH > 20 mIU/L. For the total population, median TSH and FT4 levels were 1.94 mIU/L and 1.35 ng/dL, respectively. TSH was higher and FT4 was lower in females than in males. Based on National Academy of Clinical Biochemistry criteria, the reference intervals (2.5(th) -97.5(th) percentile) were: TSH, 0.34-5.11 mIU/L; FT4, 0.98-1.79 ng/dL; TPO Ab, 5-84.88 IU/mL; Tg Ab, 10-118.2 IU/mL; and TRAb, 0.3-1.24 IU/L. With the new reference ranges, hypothyroidism was found in 4.16% of the total population (0.78% overt and 3.38% subclinical hypothyroidism) and hyperthyroidism was found in 3.18% (0.94% overt and 2.24% subclinical hyperthyroidism). Positive TPO Ab, Tg Ab, and TRAb were found in 8.96%, 12.26%, and 5.93%, respectively. The upper normal limit of TSH tended to increase with age, particularly for those aged 80 years and older. The reference interval for TSH needs to be derived from each specific population. Slightly elevated TSH concentrations in the elderly could be considered acceptable, with no need for thyroxine treatment. This article is protected by copyright. All rights reserved.
    Clinical Endocrinology 11/2013; · 3.40 Impact Factor
  • La-Or Chailurkit, Wichai Wichai Aekplakorn, Boonsong Ongphiphadhanakul
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    ABSTRACT: Although autoimmune thyroid disease in males is less common, it is unclear whether estrogen contributes to the difference in susceptibility among males. To examine if circulating estradiol is related to thyroid autoimmunity in males. One thousound two hundred and sixty three males aged 15-94 years were studied. Serum levels of 17β- estradiol (E2), thyroid-stimulating hormone receptor antibody (TRAb), thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TgAb), free thyroxine (FT4) and thyroid-stimulating hormone (TSH) were measured by electrochemiluminescence immunoassay. Circulating E2 varied widely in males, ranging 18.4-403.7 pmol/L with a mean value of 136.2 ± 51.7 pmol/L. E2 increased with age (r = 0.18, P < 0.001). No relationship between E2 and BMI was found. When comparing the difference in E2 according to the test results for TRAb, TPOAb and TgAb, it was found that E2 was significantly higher in subjects with positive TRAb (TRAb-positive, E2 = 170.3 ± 59.8 pmol/L; TRAb-negative, E2 = 134.0 ± 50.6 pmol/L; P < 0.001). No difference in E2 was demonstrated according to TPOAb or TgAb results. Logistic regression analysis showed that E2 was a determinant of positive TRAb, independent of age and BMI. There was no relationship between serum E2 and TSH or FT4. However, E2 was negatively related to TSH (r = -0.45, P < 0.01) in subjects whose TSH fell below the reference range (0.3-4.2 mIU/L). Higher circulating estradiol is related to thyroid autoimmunity in males as reflected by positive TRAb.
    European Journal of Endocrinology 10/2013; · 3.14 Impact Factor
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    ABSTRACT: Fetuin-A is associated with body mass index (BMI) as well as components of the metabolic syndrome. However, it is unclear if fetuin-A affects BMI or the other way around. We therefore assessed the causal association between fetuin-A and BMI, or vice versa utilizing a bidirectional Mendelian randomisation approach. This was a study of 2,558 subjects from the Electricity Generating Authority of Thailand (EGAT) cohort. Two polymorphisms, i.e., rs2248690 in the alpha 2 Hereman-Schmid glycoprotein (AHSG) gene and rs9939609 in the fat mass and obesity-associated (FTO) gene were genotyped. Bidirectional causal models were constructed using a two-stage least square instrumental variable (IV) regression. First, rs2248690 locus was used as the instrumental variable for the effect of circulating fetuin-A on BMI and then the FTO rs9939609 locus was used as the instrumental variable for the effect of BMI on circulating fetuin-A. Among 2,558 subjects, the prevalence of the minor AHSG (T) and FTO (A) alleles were 17.9% and 22.1%, respectively. The AHSG rs2248690 locus was highly related to serum fetuin-A levels (P < 0.001). Likewise, the FTO rs9939609 locus and BMI were highly associated (P < 0.001). Mendelian randomisation analyses showed that circulating fetuin-A, instrumented by the AHSG rs2248690 locus, was associated with BMI (coefficient = 2.26; 95% CI: 0.39, 4.12). In contrast, BMI, instrumented by the FTO rs9939609 locus, was not associated with circulating fetuin-A (coefficient = 0.0007; 95% CI: -0.0242, 0.0256). Our findings suggest a causal association leading from circulating fetuin-A to BMI. There was no evidence of reverse causality from BMI to fetuin-A. This article is protected by copyright. All rights reserved.
    Clinical Endocrinology 07/2013; · 3.40 Impact Factor
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    ABSTRACT: BACKGROUND: It is not known whether genetic variation in the vitamin D binding protein (DBP) influences 25-hydroxyvitamin D levels [25(OH)D] after vitamin D supplementation. We aimed to investigate the changes of total 25(OH)D, 25(OH)D3 and 25(OH)D2 in a Thai cohort, according to type of vitamin D supplement (vitamin D3 or D2) and DBP genotype, after receiving vitamin D3 or D2 for 3 months. METHODS: Thirty-nine healthy subjects completed the study. All subjects received 400 IU of either vitamin D3 or D2, plus a calcium supplement, every day for 3 months. Total serum 25(OH)D, 25(OH)D3 and 25(OH)D2 were measured by LC-MS/MS. Individual genotyping of rs4588 in the DBP gene was performed using real-time PCR. RESULTS: Vitamin D3 supplementation of 400 IU/d increased 25(OH)D3 significantly (+16.2 +/- 4.2 nmol/L, p <0.001). Vitamin D2 (400 IU/d) caused increased 25(OH)D2 levels (+22.0 +/- 2.11 nmol/L, p <0.001), together with a decrease of 25(OH)D3 (-14.2 +/- 2.0 nmol/L, p <0.001). At 3 month, subjects in vitamin D3 group tended to have higher total 25(OH)D levels than those in vitamin D2 (67.8 +/- 3.9 vs. 61.0 +/- 3.0 nmol/L; p = 0.08). Subjects were then classified into two subgroups: homozygous for the DBP rs4588 C allele (CC), and the rest (CA or AA). With D3 supplementation, subjects with CA or AA alleles had significantly less increase in 25(OH)D3 and total 25(OH)D when compared with those with the CC allele. However, no difference was found when the supplement was vitamin D2. CONCLUSION: Genetic variation in DBP (rs4588 SNP) influences responsiveness to vitamin D3 but not vitamin D2.
    Nutrition Journal 04/2013; 12(1):39. · 2.65 Impact Factor
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    Sunee Saetung, La-Or Chailurkit, Boonsong Ongphiphadhanakul
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    ABSTRACT: BACKGROUND: The effect of massage therapy on bone metabolism in adults has only scarcely been explored. In a randomized crossover trial, we investigated the skeletal effect of Thai traditional massage by examining the changes in biochemical markers of bone turnover. METHODS: Forty-eight postmenopausal women participated in the study. All volunteers were randomized to a 2-hour session of Thai traditional massage twice a week for 4 weeks and a 4-week control period after a 2-week washout, or vice versa. Twenty-one subjects were allocated to receiving Thai traditional massage first, followed by the control period, while 27 were initially allocated to the control period. RESULTS: Serum P1NP increased significantly after Thai traditional massage (P <0.01), while there was no change in serum osteocalcin or CTX. During the control period, there was no significant change in P1NP, osteocalcin or CTX compared to baseline. When age and height were taken into account, P1NP in postmenopausal women whose ages were in the middle and higher tertiles and whose heights were in the lower and middle tertiles (n = 22) had a 14.8 +/- 3.3% increase in P1NP after massage (P <0.001), while no change in P1NP was found in the rest of the women (n = 26). CONCLUSIONS: Thai traditional massage results in an increase in bone formation as assessed by serum P1NP, particularly in postmenopausal women who are older and have a smaller body build. Future studies with larger samples and additional design features are warranted.
    BMC Complementary and Alternative Medicine 03/2013; 13(1):69. · 2.08 Impact Factor
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    ABSTRACT: Bisphenol A (BPA) is a chemical that is used in a variety of consumer products, and exposure to BPA is widespread among the general population. Recent studies have suggested that BPA may affect the thyroid and related pathways. However, human studies are still limited. The aim of this study was to determine the relationship between BPA exposure and thyroid function. We obtained survey data and blood samples from The Thai National Health Examination Survey IV 2009, a nationally representative cross-sectional survey using a multistage, stratified sampling of the Thai population. A total of 2,340 subjects aged 18-94 years were sampled for the present study. Serum BPA, TSH, FT(4), and related covariates were measured. BPA was log-transformed prior to analysis. BPA was detected in 52.8 % of serum samples with a median concentration of 0.33 (range 0-66.91) ng/mL. We excluded subjects who tested positive for thyroid autoantibody and then stratified the remaining subjects by gender; the analysis showed a significantly negative correlation between serum BPA and FT(4) levels in males (r = -0.14, P < 0.001). In contrast, no association was observed in females. BPA was not associated with TSH in either gender. This gender-related discrepancy is possibly related to androgen-related differences in the metabolism of BPA. Our preliminary results provide evidence of a negative association between BPA and FT(4) levels. Additional detailed studies are needed to investigate the temporal relationship and potential public health implications of such an association.
    Endocrine 02/2013; · 3.53 Impact Factor
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    ABSTRACT: The purpose of this study was to investigate the association between fat mass, lean mass, and bone mineral density (BMD) in postmenopausal and perimenopausal Thai women. A cross-sectional study was conducted in 1579 healthy Thai women aged 40-90 years. Total body, lumbar spine, total femur, and femoral neck BMD and body composition were measured by dual x-ray absorptiometry. To evaluate the associations between fat mass and lean mass and various measures of BMD, multivariable linear regression models were used to estimate the regression coefficients for fat mass and lean mass, first in separate equations and then with both fat mass and lean mass in the same equation. Among the study population, 1448 subjects (91.7%) were postmenopausal and 131 (8.3%) were perimenopausal. In postmenopausal women, after controlling for age, height, and duration of menopause, both fat mass and lean mass were positively correlated with BMD when they were analyzed independently of each other. When included in the same equation, both fat mass and lean mass continued to show a positive effect, but lean mass had a significantly greater impact on BMD than fat mass at all regions except for total body. Lean mass but not fat mass had a positive effect on BMD at all skeletal sites except the lumbar spine, after controlling for age and height in perimenopausal women. Lean mass had a significant beneficial effect on BMD in both postmenopausal and perimenopausal women and can be considered as one of the determinants of bone mass. The effect of the fat mass was related to menopausal status, but only demonstrated a positive impact in perimenopausal women.
    International Journal of Women's Health 01/2013; 5:87-92.
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    ABSTRACT: OBJECTIVE: The causal effect of adipose tissue on bone mass and the direction of its net influence have not been directly assessed in adult humans. Using the Mendelian randomization analysis, we assessed the causality of adiposity in measurements of bone mass in adult males and females. DESIGN AND METHODS: Subjects consisted of 2,154 adults aged 25-54 years from a cross-sectional cohort of the employees of the Electricity Generating Authority of Thailand. Body composition was determined after at least 3 h of fasting using multi-frequency bioelectrical impedance analysis. Bone mineral density was assessed by dual energy X-ray absorptiometry. A polymorphism in the fat mass and obesity-associated gene (FTO rs9939609) was used as an instrument in the Mendelian randomization analysis. RESULTS: The genotype distribution of the FTO rs9939609 polymorphism was 61.1% TT, 33.9% AT and 5.0% AA. The average body mass index (BMI), body fat mass and percent body fat were 23.9 kg/m(2) (SD = 3.6), 17.9 kg (SD = 6.6) and 26.8% (SD = 7.2), respectively. The FTO rs9939609 polymorphism was significantly correlated with BMI (coefficient = 0.673 kg/m(2) , P < 0.001), body fat mass (coefficient = 0.948 kg, P < 0.001) and percent body fat (coefficient = 0.759%, P < 0.01). An instrumental variable (IV) regression model, using BMI as the intermediate phenotype, suggested that FTO was a strong IV. Also, the FTO-BMI polymorphism was significantly associated with total hip and femoral neck bone mineral density (BMDs) but was not correlated with total spine BMD, with estimated correlation coefficients of 0.0189 (95% CI: 0.0046, 0.0332), 0.0149 (95% CI: 0.0030, 0.0268) and 0.0025 (95% CI: -0.0131, 0.0136) g/cm(2) , respectively. The variances of BMDs explained by the FTO-BMI were 19.0%, 21.3%, and 1.1%, respectively. Similar trends were also observed for the FTO-body fat mass and FTO-percent body fat correlations. CONCLUSIONS: Mendelian randomization analysis suggests that adiposity might be causally related to bone mineral density at the femur but not at the spine.
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    ABSTRACT: OBJECTIVE: The causal effect of adipose tissue on bone mass and the direction of its net influence have not been directly assessed in adult humans. Using the Mendelian randomization analysis, we assessed the causality of adiposity in measurements of bone mass in adult males and females. DESIGN AND METHODS: Subjects consisted of 2,154 adults aged 25-54 years from a cross-sectional cohort of the employees of the Electricity Generating Authority of Thailand. Body composition was determined after at least 3 h of fasting using multi-frequency bioelectrical impedance analysis. Bone mineral density was assessed by dual energy X-ray absorptiometry. A polymorphism in the fat mass and obesity-associated gene (FTO rs9939609) was used as an instrument in the Mendelian randomization analysis. RESULTS: The genotype distribution of the FTO rs9939609 polymorphism was 61.1% TT, 33.9% AT and 5.0% AA. The average body mass index (BMI), body fat mass and percent body fat were 23.9 kg/m(2) (SD = 3.6), 17.9 kg (SD = 6.6) and 26.8% (SD = 7.2), respectively. The FTO rs9939609 polymorphism was significantly correlated with BMI (coefficient = 0.673 kg/m(2) , P < 0.001), body fat mass (coefficient = 0.948 kg, P < 0.001) and percent body fat (coefficient = 0.759%, P < 0.01). An instrumental variable (IV) regression model, using BMI as the intermediate phenotype, suggested that FTO was a strong IV. Also, the FTO-BMI polymorphism was significantly associated with total hip and femoral neck bone mineral density (BMDs) but was not correlated with total spine BMD, with estimated correlation coefficients of 0.0189 (95% CI: 0.0046, 0.0332), 0.0149 (95% CI: 0.0030, 0.0268) and 0.0025 (95% CI: -0.0131, 0.0136) g/cm(2) , respectively. The variances of BMDs explained by the FTO-BMI were 19.0%, 21.3%, and 1.1%, respectively. Similar trends were also observed for the FTO-body fat mass and FTO-percent body fat correlations. CONCLUSIONS: Mendelian randomization analysis suggests that adiposity might be causally related to bone mineral density at the femur but not at the spine. © 2012 Blackwell Publishing Ltd.
    Clinical Endocrinology 10/2012; · 3.40 Impact Factor
  • La-Or Chailurkit, Wichai Aekplakorn, Boonsong Ongphiphadhanakul
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    ABSTRACT: Background: Vitamin D is an immunomodulator and may affect autoimmune thyroid diseases. Vitamin D has also been shown to influence thyrocytes directly by attenuating the thyrotropin (TSH) stimulated iodide uptake and cell growth. However, it is unclear how vitamin D status is related to TSH at the population level. The goal of the present study was to investigate the relationship between vitamin D status and TSH levels according to thyroid autoantibodies in a population-based health survey in Thailand. Methods: A total of 2,582 adults, aged 15-98 years, were randomly selected according to geographical region from the Thailand 4th National Health Examination Survey sample. Serum levels of 25-hydroxyvitamin D (25(OH)D), TSH, thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody (TgAb) were measured in all subjects. Results: The mean age was 55.0 ± 0.4 (SE) years. Fifty percent of the subjects were males. In subjects positive for serum TgAb, serum TSH levels were higher whereas total serum 25(OH)D levels were lower. In addition, the prevalence of vitamin D insufficiency in TgAb-positive subjects was significantly higher than that observed in TPOAb and TgAb-negative subjects, whether based on cutoff values of 20 or 30 ng/mL: 8.3% vs 5.6%, p < 0.05; or 47.6% vs 42.0%, p < 0.05, respectively. However, vitamin D status was not associated with positive TPOAb and/or TgAb after controlling for gender and age. To explore the probable interaction between vitamin D status and age on serum TSH, analyses were performed according to age tertiles, it was found that higher 25(OH)D levels were independently associated with lower TSH, but only in subjects in the lowest age tertile. Conclusions: This population-based study showed that high vitamin D status in younger individuals is associated with low circulating thyrotropin.
    Thyroid: official journal of the American Thyroid Association 08/2012; · 2.60 Impact Factor
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    ABSTRACT: OBJECTIVE: To determine the diagnostic agreement and the degree of misclassification when using data from the left and right hips. METHODS: The cross-sectional study of 1,943 perimenopausal and postmenopausal Thai women, who had bone mineral density (BMD) measurements at the left (non-dominant) and right hips for the screening of low bone mass (LBM) or osteoporosis (OP) in the Department of Radiology, Faculty of Medicine, Chiang Mai University from September 2008 to August 2010 was performed. The kappa statistic was used to assess diagnostic agreement. The prevalence of LBM and OP and the percentage of misclassification were reported. RESULTS: There was a significant correlation between the left and right BMD values for the femoral neck (FN) (r (2) = 0.83; p < 0.001) and the total hip (TH) (r (2) = 0.89; p < 0.001). The diagnostic agreement of the FN and TH regions was significant in all study groups ranging from 0.69 to 0.76 (p < 0.001). For the final diagnosis, which is based on the least T-score of the FN or TH regions, the diagnostic agreement was 0.73 for all women, 0.77 for perimenopausal women, 0.73 for postmenopausal women, 0.70 for postmenopausal women age less than 65 years and 0.71 for postmenopausal women age greater than or equal to 65 years. The percentage of misclassification for all women was 16.9 %, with 3.3 % being downgraded from normal to LBM and 3.4 % from LBM to OP. CONCLUSION: Despite the fact that good diagnostic agreement was demonstrated in this study, a significant number of diagnostic discordance between left and right hips (16.9 %) was also observed. BMD measurements of both hips are recommended for diagnosing LBM and OP in clinical practice.
    Annals of Nuclear Medicine 08/2012; · 1.41 Impact Factor

Publication Stats

792 Citations
224.13 Total Impact Points

Institutions

  • 1992–2014
    • Ramathibodi Hospital
      Krung Thep, Bangkok, Thailand
  • 2011–2013
    • Chiang Mai University
      • Faculty of Medicine
      Chiang Mai, Chiang Mai Province, Thailand
  • 2012
    • Thammasat University
      • Faculty of Medicine
      Bangkok, Bangkok, Thailand
  • 1995–2012
    • Mahidol University
      • • Department of Medicine (Ramathibodi)
      • • Department of Pharmacy
      Bangkok, Bangkok, Thailand
  • 2008
    • King Mongkut's University of Technology Thonburi
      Dhonburi, Bangkok, Thailand
  • 2006
    • Naresuan University
      • Department of Pharmacy Practice
      Phitsanulok, Changwat Phitsanulok, Thailand