Hesheng Hou

Liaoning Normal University, Lü-ta-shih, Liaoning, China

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Publications (8)15.81 Total impact

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    ABSTRACT: Exogenous fragment sequence and flanking sequence between exogenous fragment and recombinant chromosome of transgenic wheat B72-8-11b were successfully acquired through PCR amplification with cross-matched primers from exogenous genes. Newly acquired exogenous fragment covered the full-length sequence of transformed genes such as transformed plasmid and corresponding functional genes including marker uidA, promoter ubiquitin, lacZ, 1Dx5, and part of sequence of the wheat genome. A specific PCR detection method for transgenic wheat B72-8-11b strain was established on the basis of primers designed according to flanking sequence. The designed primers revealed specific amplification of 132 bp product of transgenic wheat B72-8-11b strain. This method is characteristics of high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of transgenic wheat B72-8-11b strain.
    Applied biochemistry and biotechnology 01/2013; · 1.94 Impact Factor
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    ABSTRACT: In this study, the amplified fragment length polymorphism (AFLP) method was employed to estimate the genetic diversity of young sporophytes belonging to six Undaria pinnatifida populations collected from five seaweed cultivation farms along the Dalian Coast of China in 2008. A total of 397 loci were detected using ten combinations of selective primer pairs. Of these, 302 of which (76.07 %) were polymorphic, which was mainly caused by the 40 accessions of two populations collected from the LWT (44.08 %) and the JST (44.58 %) farms. According to the UPGMA dendrogram and biplot of principal component analysis, the 40 accessions represented high intra-population genetic diversity and far relationship from all of other populations. In contrast, the accessions from the other populations (HK, MS, LS, and QD) represented high intra-population similarity. The four populations may have originated from the same introduced U. pinnatifida strain. Analysis of molecular variance showed that a higher proportion of genetic variation resided among the populations (63.56 %) than that within populations (36.64 %). It is hypothesized that the differences in genetic traits, as well as the mixed mating system and reduced gene flow were the primary reasons that caused the high genetic variation among populations and the high similarity between accessions within the population. However, the genetic diversity of the partial U. pinnatifida cultivar populations in Dalian farms has been decreasing in the past years, which poses a potential risk of germplasm degradation in cultivation. In addition, the breeding techniques of cultivated U. pinnatifida in China and the application of AFLP in Undaria are discussed in this article. Based on the results, the identified polymorphic markers could be used for genetic improvement of the species through marker-assisted breeding and the results also provide excellent information for selecting indigenous gametophyte resources from Dalian farms using different breeding methods.
    Journal of Applied Phycology 01/2013; 25(4). · 2.33 Impact Factor
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    ABSTRACT: Enhanced fucosyltransferase IV (FUT4) expression correlates with increased tumor malignancy in many carcinomas. However, little is known about the regulation of FUT4 expression, and whether FUT4 expression is influenced by the methylation status of the FUT4 promoter is unclear. In this study, we demonstrated that FUT4 expression is negatively correlated with the methylation degree of a CpG island in the FUT4 promoter, suggesting that the methylation status of FUT4 promoter regulates the expression of FUT4. The results indicate that manipulating the methylation status of the FUT4 promoter to regulate FUT4 expression may be a novel approach in the treatment of malignant tumors.
    Cellular & Molecular Biology Letters 01/2012; 17(2):206-16. · 1.95 Impact Factor
  • Xueyan Zhong, Hesheng Hou, Wenping Qiu
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    ABSTRACT: Recombinant plant viruses have the propensity to remove foreign inserts during replication. This process is virus-specific and occurs in a host-dependent manner. In the present study, we investigated the integrity of foreign inserts in recombinant plant viruses using a model system consisting of Tomato bushy stunt virus (TBSV) and its defective interfering RNA (DI). These were tested in Nicotiana benthamiana plants that were either wild type or transgenic for the green fluorescent protein (GFP) gene. GFP-derived inserts were retained in the recombinant TBSV and DI population that were inoculated onto GFP-transgenic N. benthamiana plants in which silencing of the GFP transgene was initiated, but they were removed from the virus and DIs that were maintained on wild-type plants. A foreign insert derived from an endogenous N. benthamiana gene encoding the H subunit of the magnesium chelatase (NbChlH) was deleted, whereas the fragment of an RNA-dependent RNA polymerase gene (NbRdRP1m) was retained in the recombinant TBSV population. These results demonstrate that the recombination of TBSV to remove nonviral fragments is influenced by silencing and the type of inserts.
    Molecular Plant-Microbe Interactions 09/2005; 18(8):800-7. · 4.31 Impact Factor
  • Hesheng Hou, Wenping Qiu
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    ABSTRACT: Virus induced gene silencing (VIGS) and suppression are RNA-specific defense and counter-defense circuits in plant-virus interactions. These phenomena have been investigated extensively with an Agrobacterium-mediated transient expression system. In this study, a virus-based transient expression system was developed to study these phenomena. A Tomato bushy stunt virus (TBSV) viral vector with an inactivated P19 suppressor gene, referred to as pHST2-14, was chosen to express the P1 of Tobacco etch virus (TEV). TEV P1 is a component of a well-characterized VIGS suppressor, TEV P1/HC-Pro protein. A TBSV defective interfering RNA (DI) that contains the 3' proximal portion of a green fluorescence protein (GFP) gene, DI-P, was used as a silencing inducer of the homologous GFP gene on GFP transgenic Nicotiana benthamiana (NbGFP) plants. The TEV P1 gene was inserted into pHST2-14 to generate TBSV-P1. Transcripts of TBSV-P1 were then mixed with DI-P transcripts and inoculated onto NbGFP plants. DI-P consistently accumulated in NbGFP plants that were inoculated with TBSV-P1 and DI-P, and efficiently induced silencing of GFP transgene. These results demonstrate that a TBSV-based co-delivery system can provide a new alternative tool to investigate gene silencing and its influence by a TBSV-expressed foreign protein. It also can be used to elucidate functions of endogenous genes in plants.
    Journal of Virological Methods 08/2003; 111(1):37-42. · 1.90 Impact Factor
  • Y. Song, Y. Lin, S. Tong, H. Hou
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    ABSTRACT: Ethylene response factors (ERFs) are involved in many plant development events and stress defenses. In this study, an ERF gene, VvERF3b, was cloned from the leaves of Vitis vinifera. VvERF3b belongs to ERF group VIIIa. Expression of the gene was induced by abscisic acid, ethephon, and salicylic acid, but not by NaCl. Promoter sequence analysis of the VvERF3b gene revealed that there are several potential cis-acting elements that may be potentially recognized and bound by the transcription factors related to hormones and stress responses. Deletion analysis showed that the 5′-flanking sequence of −1047 to −585 from the transcriptional start site is essential to the high expression of the VvERF3b gene, whereas the sequence fragment of −1324 to −1047 revealed suppression effect. The result indicated that the region appears to contain cis-acting elements that can be bound by the proteins in a transcription complex to induce the inhibition of gene expression.
    Biologia Plantarum 56(1). · 1.69 Impact Factor
  • Y. Song, Y. Lin, S. Tong, H. Hou
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    ABSTRACT: Ethylene response factors (ERFs) are involved in many plant development events and stress defenses. In this study, an ERF gene, VvERF3b, was cloned from the leaves of Vitis vinifera. VvERF3b belongs to ERF group VIIIa. Expression of the gene was induced by abscisic acid, ethephon, and salicylic acid, but not by NaCl. Promoter sequence analysis of the VvERF3b gene revealed that there are several potential cis-acting elements that may be potentially recognized and bound by the transcription factors related to hormones and stress responses. Deletion analysis showed that the 5′-flanking sequence of −1047 to −585 from the transcriptional start site is essential to the high expression of the VvERF3b gene, whereas the sequence fragment of −1324 to −1047 revealed suppression effect. The result indicated that the region appears to contain cis-acting elements that can be bound by the proteins in a transcription complex to induce the inhibition of gene expression.
    Biologia Plantarum · 1.69 Impact Factor
  • Hesheng Hou, Wenping Qiu, Laszlo Kovacs
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    ABSTRACT: Construction of Norton grapevine leaf cDNA library and single-read sequencing. Total RNA was isolated from fully expanded leaves of greenhouse-grown Norton grapevines by a previous protocol (Malnoy et al., 2001). Poly(A) + mRNA was purified by using the Poly(A) Purist Kit from Ambion (Austin, TX). The mRNAs were transcribed into cDNA using oligo(dT) linker/primers and were ligated to EcoRI adaptors at 5'-ends. The cDNAs were cloned into the XhoI-EcoRI site of the pBS II SK(+) phagemid. The cDNA library was constructed by using the cDNA library Construction Kit from Stratagene (La Jolla, CA). The 192 randomly selected cDNA clones were single-read sequenced from 5'-ends at Qiagen Genomics (Bothell, WA). The vector sequences were trimmed off in the ContigExpress program of NTI Vector Suite (Informax). Norton grapevine, V. aestivalis var. Norton, is highly resistant to several fungal diseases (Figure 1). On this poster, we reported the cloning of three members of the stress-induced stilbene syn-thase gene family and construction of a cDNA library from young leaf tissues of Norton grapevine. We presented the results of sequence comparisons between stilbene synthase genes from V. aestivalis var. Norton and V. vinifera, and the initial functional annotation of 121 expressed sequence tags (ESTs) from Norton young leaf tissues.