[Show abstract][Hide abstract] ABSTRACT: To study the role of Chondromodulin I (ChM-I) in inducing bone mesenchymal stem cells (MSCs) into chondrocytes, the plasmid expressing pcDNA3.1 (+) / ChM-I was constructed, and transfected into MSCs. Stable expression verified that ChM-I is upregulated in MSCs, which make it a useful preparation for studying the further role of ChM-I in inducing bone MSCs toward a chondrogenic I phenotype and for tissue engineering constructs. MSCs were obtained from adult Sprague Dawley (SD) rats using density gradient centrifugation. Cell surface antigens were detected by flow cytometry. The experimental groups were MSCs transfected with the plasmid pcDNA3.1 (+)/ChM-I. The control one was with the plasmid pcDNA3.1 (+) and the negative control one was with no plasmid. Transcription and translation were detected in the level of mRNA and protein respectively in order to identify the stability of the expression of ChM-I in cell lines by RT-PCR and western blot analysis. The results of RT-PCR showed that the mRNA level of ChM-I in the experimental group was six times higher than the control. The quantitative optical density analysis showed that the expression of ChM-I in the experimental group was significantly higher than the control by western blot. We conclude that we have established MSCs lines stably expressing ChM-I. This work lays the foundation of studying the potential role of the ChM-I in inducing BMSCs into chondrocytes.
International journal of clinical and experimental medicine 01/2012; 5(1):34-43.
[Show abstract][Hide abstract] ABSTRACT: To explore an effective method of correction of deviated nose and functional reconstructive nasal surgery.
Among 102 patients 32 cases were treated by septoplasty only, 53 cases were corrected by bony pyramid, 3 cases with severe asymmetry of bony pyramid by wedge resection and 14 cases by the implant of the septal or rip cartilage.
The successful rate was 92.20% (92/102) with period of 6 to 24 months follow up.
Correcting the deviated nose to reconstruct its function with the intranasal approach and multiple techniques, can benefit both the cosmetology and nasal functional improvement. Rectifying the nasal septum, debonding the tissue of the deviated section completely, and fixing reconstructive structures are the keys for the successful result.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 09/2010; 24(17):788-91.