Masahiro Miyake

Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States

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Publications (23)70.91 Total impact

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    ABSTRACT: To investigate the 2-year outcomes of intravitreal injections of ranibizumab in typical neovascular age-related macular degeneration (tAMD) and polypoidal choroidal vasculopathy (PCV). Factors associated with visual outcomes are examined.
    06/2014;
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    ABSTRACT: Corneal curvature measures the steepness of the cornea and is an important parameter for clinically diseases such as astigmatism and myopia. Despite the high heritability of corneal curvature, only two associated genes have been discovered to date. We performed a three-stage genome-wide association study meta-analysis in 12,660 Asian individuals. Our Stage 1 was done in multi-ethnic cohorts comprising 7,440 individuals, followed by a Stage 2 replication in 2,473 Chinese and Stage 3 in 2,747 Japanese. The SNP array genotype data were imputed up to the 1000 Genomes Project Phase 1 cosmopolitan panel. The SNP association with the radii of corneal curvature was investigated in the linear regression model with the adjustment of age, gender, and principal components. In addition to the known genes, MTOR (also known as FRAP1) and PDGFRA, we discovered two novel genes associated with corneal curvature: CMPK1 (rs17103186, P=3.3 x 10(-12)) and RBP3 (rs11204213 [Val884Met], P=1.1 x 10(-13)). The missense RBP3 SNP, rs11204213, was also associated with axial length (P=4.2 x 10(-6)) and had larger effects on both corneal curvature and axial length compared to other SNPs. The index SNPs at the four indicated loci explained 1.9% of corneal curvature variance across the Stages 1 and 2 cohorts, while 33.8% of corneal curvature variance was explained by the genome-wide imputation data. We identified two novel genes influencing corneal curvature, which are related to either corneal shape or eye size. This study provides additional insights into genetic architecture of corneal shape.
    Human Molecular Genetics 06/2014; · 7.69 Impact Factor
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    ABSTRACT: To investigate the 2-year outcomes of photodynamic therapy (PDT) combined with intravitreal injections of ranibizumab for polypoidal choroidal vasculopathy (PCV).
    05/2014;
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    ABSTRACT: To compare and analyze differences and similarities between Japanese and French patients in subtype diagnosis of exudative age-related macular degeneration (AMD) as determined by fundus photography (FP) and fluorescein angiography (FA), and a multimodal imaging involving FP, FA, indocyanine green angiography (ICGA), and optical coherence tomography (OCT).
    American journal of ophthalmology. 05/2014;
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    ABSTRACT: Purpose: To describe the clinical and genetic characteristics of choroidal neovascularization (CNV) in eyes with choroidal vascular hyperpermeability (CVH). Methods: This cross-sectional study consisted of 438 consecutive patients who underwent fluorescein and indocyanine green angiography for macular disease. We used the genotypes of 1,576 age-related macular degeneration (AMD) cases and 3,248 general population controls as reference groups for genetic association analyses. Results: Of 871 eyes (438 patients) examined, CVH was found in 227 eyes (26.1%). Of these 227 eyes, 52 (22.6%) had CNV in the macular area. The proportion of patients with drusen and the choroidal thickness were not different between eyes with and without CNV, after adjusting for age (P = 0.21, and 0.95). Of the 52 eyes with CNV, 51 had type 1 CNV and only one eye had pure type 2 CNV. Of the 51 eyes with type 1 CNV, polypoidal lesions were observed in 17 eyes (33.3%). Genotype distributions of ARMS2 (A69S) and CFH (I62V) in patients with CVH and type 1 CNV significantly differed from those of AMD cases (P = 0.0014 and 0.0098, respectively), but not from general population controls (P = 0.33 and 0.82; statistical power of 88.5% and 72.9%, respectively). Conclusions: In patients with CVH, type 1 CNV may occur frequently and sometimes accompanies type 2 CNV or polypoidal lesions. In terms of ARMS2 and CFH, genetic background of patients with CVH and type 1 CNV was different from those with AMD, but rather similar to the general Japanese population.
    Investigative ophthalmology & visual science 04/2014; · 3.43 Impact Factor
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    ABSTRACT: Purpose To determine if asymmetry in thickness of the retinal nerve fiber layer (RNFL), ganglion cell layer, ganglion cell complex, and total retina between upper and lower macula halves can predict glaucoma. Design Retrospective case-control series. Methods One hundred twenty-two eyes of 122 patients (30 normal eyes and 30 preperimetric, 31 early, and 31 advanced glaucoma eyes) were studied. The RNFL, ganglion cell layer, ganglion cell complex, and total retina were segmented and measured on 10 vertical B-scans over a 30°×15° macular area. The equation asymmetry index =|log10 (lower hemiretinal thickness/upper hemiretinal thickness)| was used to calculate asymmetry indices for 8 pairs of upper and lower 0.5-mm segments equidistant from the fovea on each scan. Areas under the receiver operating characteristic curve (AROCs) for mean thickness and mean asymmetry index of 10 B-scans were compared. Results The overlap in values for normal and glaucomatous eyes was minimal for the ganglion cell layer asymmetry index. Thickness parameters decreased with the severity of glaucoma, whereas asymmetry indices did not. AROCs for thickness measurements tended to increase with increasing glaucoma severity (preperimetric, 0.746–0.808; early, 0.842–0.940; advanced, 0.943–0.995), whereas AROCs for asymmetry indices did not have distinct ranges according to glaucoma severity (advanced, 0.819–0.996; early, 0.861–0.998; preperimetric, 0.773–0.994). The AROC for the ganglion cell layer asymmetry index remained almost perfect regardless of glaucoma severity (0.994–0.998). Conclusions Macular retinal layer thickness asymmetry indices, particularly for the ganglion cell layer, show promise as early indicators of glaucomatous retinal damage.
    American Journal of Ophthalmology. 01/2014;
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    ABSTRACT: To evaluate macular function using multimodality in eyes with age-related macular degeneration (AMD) at various stages. Macular function in 20 control eyes (20 subjects), 17 eyes (17 patients) with large drusen, 18 eyes (18 patients) with drusenoid pigment epithelial detachment (PED), and 19 eyes (19 patients) with neovascular AMD was examined using a Landolt chart for visual acuity; retinal sensitivity was measured by microperimetry; and focal macular electroretinography (fmERG) was performed. In all of these eyes, retinal morphology was examined using optical coherence tomography. Eyes with neovascular AMD showed morphologic changes in the neurosensory retina as well as marked deterioration of macular function in all parameters measured with a Landolt chart, fmERG, and microperimetry. Eyes with large drusen showed only minimal morphologic changes in the neurosensory retina. In this large drusen group, although retinal sensitivity at the central point was significantly decreased (P = 0.0063), the other parameters of macular function were well preserved. In eyes with drusenoid PED, the structure of the neurosensory retina was well preserved, while the foveal thickness was significantly increased (P = 0.013). The macular function of these eyes was significantly deteriorated, with the VA, amplitude of the a-wave and b-wave, and retinal sensitivity being markedly decreased. In addition, the area of PED correlated with the latency of the a-wave and b-wave and with the retinal sensitivity within the central 4° or 8° region. Multimodal evaluation demonstrated a significant decrease in macular function in drusenoid PED and in neovascular AMD.
    Japanese Journal of Ophthalmology 12/2013; · 1.27 Impact Factor
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    ABSTRACT: Purpose:We investigated the association of genetic variations, which were recently identified in a large-scale genome-wide association study (GWAS) to confer risk of refractive error and common myopia in Caucasians, with high myopia in Japanese subjects. Methods:The 5 single-nucleotide polymorphisms (SNPs) from the 5 genes TOX, RDH5, ZIC2, RASGRF1, and SHISA6 were genotyped in 1339 unrelated highly myopic Japanese patients and 3248 healthy Japanese participants in the Nagahama Study. In addition, genotypes were compared between high myopia patients without choroidal neovascularization (CNV) and patients with myopic CNV. Results:Significant associations between rs8000973 near ZIC2 (P = 8.80 × 10-10), rs4778879 in RASGRF1 (P = 3.85 × 10-7), and rs2969180 in SHISA6 (P = 0.028) and high myopia were observed. Odds ratios (95% confidence intervals) were 1.39 (1.25-1.54), 0.78 (0.71-0.86), and 1.11 (1.01-1.22) for the rs8000973 C allele, rs4778879 A allele, and rs2969180 G allele, respectively. The effect of the rs2969180 allele G contrasted with that observed in the original report, whereas the effect of the other 2 SNPs agreed. Further analysis using controls with -1.0D ≤ spherical equivalent ≤ +1.0D showed a significant association between ZIC2 and RASGRF1, but not SHISA6. Among the patients with high myopia, 516 had myopic CNV in either eye, while 823 patients did not have myopic CNV in both eyes. No evaluated genes showed a significant association with the development of myopic CNV. Conclusions:ZIC2 and RASGRF1 are susceptibility genes not only for common myopia, but also for high myopia.
    Investigative ophthalmology & visual science 10/2013; · 3.43 Impact Factor
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    ABSTRACT: Structured Abstract Purpose: To determine whether genetic variants in the lipid-associated genes are associated with the risk of developing polypoidal choroidal vasculopathy (PCV) in a Japanese population. Methods: Five-hundred-eighty-one patients with PCV and 793 controls were enrolled in this study. Association analysis of allele and genotype frequencies was performed for the following single-nucleotide polymorphisms (SNPs), which are associated with high-density lipoprotein (HDL) cholesterol levels in blood: rs493258 at hepatic lipase gene (LIPC), rs3764261 at cholesteryl ester transfer protein gene (CETP), and rs12678919 at lipoprotein lipase gene (LPL). A further model adjusting for age-related maculopathy susceptibility 2 (ARMS2) A69S, complement factor H (CFH) I62V, age, gender, and smoking status was used to confirm the independent association of these SNPs from other covariates. Results: CETP rs3764261 was significantly associated with developing PCV; the frequency of the minor allele A was higher in the PCV cases (24.0%) than in the control subjects (18.5%, P = 0.0025, odds ratio [OR] = 1.41, 95% confidence interval = 1.13-1.75). Furthermore, we found an independent association of CETP variants from age, gender, smoking, and genetic background of ARMS2 A69S, CFH I62V, LIPC rs493258, and LPL rs12678919 (P = 0.0013, OR = 1.50). LIPC rs493258 and LPL rs12678919 did not show significant associations for developing PCV (P > 0.05). Conclusion: CETP variants are associated with the risk of developing PCV in the Japanese population.
    Investigative ophthalmology & visual science 08/2013; · 3.43 Impact Factor
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    ABSTRACT: To investigate the association between the vascular endothelial growth factor (VEGF) gene polymorphism and the response to anti-VEGF treatment for choroidal neovascularization (CNV) in highly myopic eyes. Retrospective cohort study. A total of 357 unrelated highly myopic Japanese patients with axial lengths ≥26.0 mm in both eyes were eligible, and 83 patients who received anti-VEGF therapy for CNV and could be followed for more than 1 year were included. We genotyped a functional single nucleotide polymorphism in the VEGF gene, rs2010963. The associations between the distribution of the rs2010963 genotype and the number of eyes with maintained or improved visual acuity (VA) were analyzed. Furthermore, multivariable logistic regression analysis was performed to adjust for 7 possible prognostic factors, including age, sex, CNV size, CNV location, administration of loading dose, pretreatment VA, and number of additional treatments. The primary end point was maintenance of VA, and secondary end points were progression of chorioretinal atrophy (CRA) and recurrence of CNV. Mean age and mean axial length were not significantly different among 3 genotypes of rs2010963. The percentage of eyes with maintained or improved VA was significantly higher with the G allele of rs2010963 (P =0.016), and stepwise analysis revealed that both rs2010963 and CNV size were associated with VA maintenance (P =0.040 and 0.033, respectively). The secondary analysis revealed that administration of a loading dose was significantly associated with both CRA progression (P =0.031) and recurrence of CNV (P =0.020), whereas rs2010963 was not. These results suggest that the VEGF polymorphism influences the VA prognosis in highly myopic eyes with CNV within 1 year after anti-VEGF treatment. This association was still observed after removing its confounding effect through CNV size. The rs2010963 polymorphism was not associated with CNV recurrence or CRA progression, which indicates that these changes are not tied to intrinsic factors and may be controllable by improving treatment methods. The author(s) have no proprietary or commercial interest in any materials discussed in this article.
    Ophthalmology 08/2013; · 5.56 Impact Factor
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    ABSTRACT: To study the prevalence and 3-dimensional (3-D) tomographic features of focal choroidal excavations in eyes with central serous chorioretinopathy (CSC) using swept-source optical coherence tomography (OCT). Prospective, cross-sectional study. We examined 116 consecutive eyes with CSC with a prototype 3-D swept-source OCT. 3-D images of the shape of the macular area, covering 6 × 6 mm(2), were reconstructed by segmentation of the outer surface of the retinal pigment epithelium (RPE). The 3-D swept-source OCT detected focal choroidal excavations in 9 eyes (7.8%). The 3-D scanning protocol, coupled with en face scans, allowed for clear visualization of the excavation morphology. In 5 eyes with focal excavations, unusual choroidal tissue was found beneath the excavation, bridging the bottom of the excavation and the outer choroidal boundary. Additionally, 3 of those 5 eyes showed a suprachoroidal space below the excavation, as if the outer choroidal boundary is pulled inward by this bridging tissue. The focal choroidal excavations were located within fluorescein leakage points and areas of choroidal hyperpermeability. Eyes with focal choroidal excavations were more myopic (-4.42 ± 2.92 diopters) than eyes without excavations (-0.27 ± 1.80 diopters, P = .001). Subfoveal choroidal thickness was significantly thinner (301.3 ± 60.1 μm) in eyes with focal excavations than in eyes without the excavations (376.6 ± 104.8 μm, P = .036). Focal choroidal excavations were present in 7.8% of eyes with CSC. In these eyes, focal choroidal excavations may have formed from RPE retraction caused by focal scarring of choroidal connective tissue.
    American journal of ophthalmology 07/2013; · 3.83 Impact Factor
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    ABSTRACT: PURPOSE: We conducted a case-control study in a relatively large cohort of highly myopic patients to explore the genetic background of the occurrence of choroidal neovascularization (CNV) secondary to high myopia. METHODS: We evaluated 3 single nucleotide polymorphisms (SNPs) from 2 candidate genes: pigment epithelium-derived factor (PEDF) and complement factor I (CFI). The SNPs were selected based on previous reports. A total of 1082 unrelated highly myopic (i.e., axial length ≥ 26 mm in at least one eye) Japanese individuals with CNV (n = 478) and without CNV (n = 557) who were ≥50 years of age were genotyped by using the Taqman SNP assay. Multivariable logistic regression was conducted to adjust for age, sex, and axial length. RESULTS: Compared to individuals without CNV, subjects with CNV were significantly older (P < 0.01) and more likely to be female (P < 0.01), but they did not have a significantly different axial length (P = 0.50). We did not find an association between the 3 SNPs and the occurrence of CNV. However, a subanalysis using extremely myopic patients (case: control = 284:317) revealed a marginal association of rs12603825 in the PEDF gene (P = 0.045). The contribution of rs1136287 in CFI was not found in any analysis. CONCLUSIONS: We demonstrated a marginal association of the PEDF SNP, rs12603825, with myopic CNV in extremely myopic patients. A further study using a larger cohort might elucidate a significant association. Rs1136287 in CFI is less likely to be associated in Japanese individuals.
    Investigative ophthalmology & visual science 05/2013; · 3.43 Impact Factor
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    ABSTRACT: Purpose. To evaluate changes in macular function in eyes with polypoidal choroidal vasculopathy (PCV) after intravitreal ranibizumab (IVR) treatment. Methods. Twenty-three eyes from 23 patients with treatment-naive subfoveal PCV received 3 monthly injections of IVR, followed by as-needed injections. Visual acuity (VA), retinal thickness (measured with optical coherence tomography), macular sensitivity (measured with microperimetry), and focal macular electroretinograms (fmERGs) were evaluated both before the initiation of therapy and after 3 and 12 months. Results. Before treatment, cystoid macular edema was observed in 5 eyes, serous retinal detachments in 13 eyes, and serosanguinous pigment epithelial detachments in 18 eyes. IVR treatment resulted in substantial morphological improvements and consequent marked reductions in foveal thickness (P = 0.008). Although logMAR VA did not improve significantly over the 12-month study period (P = 0.623), the amplitude of the fmERG photopic negative response and macular sensitivity within 4 degree had increased significantly at 3 months (P = 0.004, P = 0.026, respectively). This trend persisted until the end of the 12-month monitoring period. Among the eyes with preexisting serous retinal detachments, those in which the detachments had resolved completely at 3 months also exhibited greater increases in fmERG a-wave amplitudes (P = 0.048). Conclusion. IVR therapy resulted in morphological improvements and the partial recovery of macular function in eyes with subfoveal PCV. This therapy may improve photoreceptor function by resolving serous retinal detachments.
    Investigative ophthalmology & visual science 05/2013; · 3.43 Impact Factor
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    ABSTRACT: Severe myopia (defined as spherical equivalent<-6.0 Diopters) is a predominant problem in Asian countries, resulting in substantial morbidity. We performed a meta-analysis of four genome-wide association studies (GWAS), all of East Asian descent totaling 1,603 cases and 3,427 controls. Two SNPs (rs13382811 from ZFHX1B [encoding for ZEB2] and rs6469937 from SNTB1) showed highly suggestive evidence of association with disease (P< 1 x 10-7) and were brought forward for replication analysis in a further 1,241 severe myopia cases and 3,559 controls from a further three independent sample collections. Significant evidence of replication was observed, and both SNP markers surpassed the formal threshold for genome-wide significance upon meta-analysis of both discovery and replication stages (P=5.79 x 10-10, per-allele OR=1.26 for rs13382811 and P=2.01 x 10-9, per-allele OR=0.79 for rs6469937). The observation at SNTB1 is confirmatory of a very recent GWAS on severe myopia. Both genes were expressed in the human retina, sclera, as well as the retinal pigmented epithelium. In an experimental mouse model for myopia, we observed significant alterations to gene and protein expression in the retina and sclera of the unilateral induced myopic eyes for Zfhx1b and Sntb1. These new data advances our understanding of the molecular pathogenesis of severe myopia.
    Human Molecular Genetics 01/2013; · 7.69 Impact Factor
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    ABSTRACT: PURPOSE: To estimate the age- and sex-specific prevalence of early age-related macular degeneration (AMD; drusen and retinal pigment abnormalities) and late AMD (exudative AMD and geographic atrophy) in the Japanese population. DESIGN: Community-based, cross-sectional study. METHODS: The study was held in Nagahama, Japan, and included 6065 Japanese individuals (aged >/=50 years) recruited in 2008-2010. We graded fundus photographs of both eyes for the AMD phenotype based on drusen size, the presence of retinal pigment abnormalities, and late AMD. The associations between smoking and AMD phenotypes were also evaluated. RESULTS: We assessed 5595 subjects (women, 65%) with a gradable macular condition. Early and late AMD prevalence increased from 16.1% and 0.27% at 50-59 years to 31.2% and 0.98%, respectively, at 70-74 years and was predominant in male subjects in each age group. Smoking was associated with both early and late AMD stages and retinal pigment abnormalities (P < .0001), but not with drusen (P = .305). The prevalence of retinal pigment abnormalities was significantly higher in men (P < .0001), which was associated with high rates of cigarette smoking. We found no sex difference for the prevalence of large drusen (P = .264). CONCLUSIONS: The prevalence of early AMD among adult Japanese persons was similar to the rates in white populations. The prevalence of late AMD in Japanese people aged <70 years was similar to that observed in white populations, whereas that in Japanese people aged >/=70 years was relatively lower.
    American journal of ophthalmology 01/2013; · 3.83 Impact Factor
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    ABSTRACT: To investigate whether genetic variations in the insulin-like growth factor 1 (IGF-1) gene are associated with high myopia in Japanese. A total of 1,339 unrelated Japanese patients with high myopia (axial length ≥26 mm in both eyes) and two independent control groups were evaluated (334 cataract patients without high myopia and 1,194 healthy Japanese individuals). The mean axial length (mm±SD) in the case group was 29.18±1.85 mm, and the mean spherical equivalent (D±SD) of the phakic eyes was -12.69±4.54 D. We genotyped five tagging single nucleotide polymorphisms (SNPs) in IGF-1: rs6214, rs978458, rs5742632, rs12423791, and rs2162679. Chi-square tests for trend, multivariable logistic regression, and haplotype regression analysis were conducted. We found no significant association between the IGF-1 SNPs and high or extreme myopia (axial length ≥28 mm in both eyes, 837 subjects) in the additive model, even when compared with the cataract and general population controls, with or without adjustments for age and sex. The evaluation using dominant and recessive models also did not reveal any significant associations. The haplotype analysis with a variable-sized sliding-window strategy also showed a lack of association of IGF-1 SNPs with high or extreme myopia. The results of the present study using a Japanese subset do not support the proposal that the IGF-1 gene determines susceptibility to high or extreme myopia in Caucasians and Chinese. Further studies are needed to confirm our reports in other populations and to identify the underlying genetic determinants of these ocular pathological conditions.
    Molecular vision 01/2013; 19:1074-81. · 1.99 Impact Factor
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    ABSTRACT: PURPOSE: To study the tomographic and pathomorphologic features of dome-shaped maculas with swept-source optical coherence tomography (OCT). DESIGN: Prospective, cross-sectional study. METHODS: The macular area of 51 highly myopic eyes (35 patients) with dome-shaped maculas was studied with swept-source OCT at 1050 nm. Three-dimensional (3-D) data sets were obtained with raster scanning covering a 12 × 8-mm(2) area; 3-D images of the posterior pole were constructed by autosegmentation of the retinal pigment epithelium (RPE). RESULTS: In all reconstructed 3-D images of the RPE, 2 outward concavities were seen within the posterior staphyloma and a horizontal ridge was formed between these 2 concavities. In 42 of these eyes, this horizontal ridge was band shaped. The vertical OCT section through the fovea showed a convex configuration of RPE, but the horizontal section showed an almost flat RPE line. In 9 eyes, 3-D images showed a typical dome-shaped convexity within the staphyloma. OCT scans showed no outward protrusions in the external scleral surface, but marked scleral thinning was seen consistent with the 2 outward concavities of the RPE. The sclera of the fovea (518.6 ± 97.6 μm) was significantly thicker than that in all 4 quadrants of the parafoveal area (range, 277.2 to 360.3 μm; P < .001). CONCLUSIONS: In highly myopic eyes with a dome-shaped macula, a horizontal ridge is formed within the posterior staphyloma by uneven thinning of the sclera.
    American journal of ophthalmology 11/2012; · 3.83 Impact Factor
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    ABSTRACT: PURPOSE: To study the choroidal thickness in eyes with inferior posterior staphyloma (IPS) and to elucidate their role in the development of macular complications. METHODS: The macular area of 42 eyes of 32 patients with IPS was studied prospectively with swept source optical coherence tomography at 1,050 nm. Using a raster scan protocol with 512 × 128 A-scans, we produced a macular choroidal thickness map (6 × 6-mm2). RESULTS: Eyes with IPS showed relatively well-preserved choroid outside of the staphyloma but the inferior-nasal choroid within the staphyloma was thinned substantially. In addition, eyes with IPS often had a belt-shaped area with the thinnest choroid along the superior border of the staphyloma. As patient age increased, choroidal thinning progressed in the entire macular area. The macular choroidal thickness showed a close correlation with age (R2 = 0.506, P < 0.001). On the superior border of the staphyloma, 13 (30.9%) eyes showed serous retinal detachment and/or pigment epithelial detachment without neovascularization, and 8 (19.0%) showed neovascularization. Patients with neovascularization were older and had worse visual acuity (P < 0.001). Macular choroidal thickness in eyes with neovascular complications (76.5 ± 19.9 µm) was significantly reduced compared with that of eyes with no complication (133.0 ± 61.9 µm, P = 0.035). CONCLUSIONS: Eyes with IPS showed marked choroidal thinning along the superior border of the staphyloma. Reduction of the choroidal thickness progressed with age and seems to be involved in the development of neovascularization associated with the IPS.
    Investigative ophthalmology & visual science 10/2012; · 3.43 Impact Factor
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    ABSTRACT: Purpose. To evaluate the association of genetic variants at chromosomes 8p21 and 4q12 with the risk of developing AMD and its two main subtypes, choroidal neovascular membrane (CNV) and polypoidal choroidal vasculopathy (PCV), in Asian populations. Methods. The study population comprised 2360 patients with neovascular AMD (1013 typical AMD-CNV and 1282 PCV), and 3598 controls from four independent cohorts, two of Japanese (n = 4859) and two of Chinese (n = 1099) ethnicity. We performed a meta-analysis in case-control studies of two reported single nucleotide polymorphisms (SNPs) (rs13278062 at TNFRSF10A-LOC389641 on 8p21 and rs1713985 at REST-C4orf14-POLR2B-IGFBP7 on 4q12) by using logistic regression analysis adjusted for age and sex. Subgroup analysis by CNV and PCV subtypes were performed to evaluate the significance of these two variants. Results. The reported association between rs13278062 at 8p21 and neovascular AMD was replicated in this population (P = 1.12 × 10(-4), odds ratio [OR] = 0.79, 95% confidence interval [CI] = 0.70-0.89). However, there was no association of rs1713985 at 4q12 with neovascular AMD, or its two subtypes, typical AMD-CNV and PCV (all P > 0.05). The study sample size had a statistical power of greater than 99% to detect an association of a risk allele with AMD with an OR of 1.30, as reported in the original study of rs1713985 and AMD. Conclusions. The present results did not replicate the reported association between rs1713985 at 4q12 and neovascular AMD. However, we confirmed the association between rs13278062 at 8p21 and neovascular AMD in Asian populations.
    Investigative ophthalmology & visual science 08/2012; 53(10):6576-81. · 3.43 Impact Factor
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    ABSTRACT: PURPOSE: To investigate the long-term visual prognosis and progression of chorioretinal atrophy in patients with myopic choroidal neovascularization (mCNV) treated with intravitreal injections of bevacizumab. METHODS: Hospital-based, retrospective, cross-sectional study. In total, 22 patients (22 eyes) with treatment-naïve mCNV who underwent intravitreal injection of bevacizumab and were followed up for more than 48 months were investigated. Visual acuity and fundus photographs before and 1, 2, 3, and 4 years after initial treatment in the clinics were compared and judged if chorioretinal atrophy (CRA) developed/enlarged or remained unchanged. The influence of clinical characteristics including age, sex, axial length, baseline visual acuity, CNV area, CNV location, and number of injections were investigated with logistic regression analysis. RESULTS: Mean logarithm of the minimum angle of resolution (logMAR) improved from 0.76 to 0.52 (P < .01), 0.48 (P < .01), and 0.54 (P < .05) after 1, 2, and 3 years, respectively. The effect slightly declined to marginally non-significant levels after 4 years (logMAR, 0.59; P = .07). CRA developed or enlarged in nine cases (41 %) in 1 year, reaching 16 cases (73 %) at the final visit. Those without CRA enlargement achieved better visual improvement. None of the aforementioned patient characteristics significantly affected CRA. CONCLUSIONS: Anti-VEGF therapy for mCNV is effective for vision improvement in the long term. On the other hand, development or enlargement of CRA frequently occurred, and affected visual improvement. Strategies to manage atrophy should be the next step in achieving better visual outcome upon mCNV treatment.
    Albrecht von Graæes Archiv für Ophthalmologie 04/2012; · 1.93 Impact Factor

Publication Stats

49 Citations
70.91 Total Impact Points

Institutions

  • 2013
    • Massachusetts Eye and Ear Infirmary
      Boston, Massachusetts, United States
  • 2012–2013
    • Suez Canal University
      • Department of Ophthalmology
      Ismailia, Muhafazat al Isma`iliyah, Egypt
    • Kyoto University
      • Department of Ophthalmology and Visual Sciences
      Kyoto, Kyoto-fu, Japan