Ryan A Colyer

Publications (3)26.91 Total impact

• Article: Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins.

  Soohong Kim, Anna Gottfried, Ron R Lin, Thomas Dertinger, Andrew S Kim, Sangyoon Chung, Ryan A Colyer, Elmar Weinhold, Shimon Weiss, Yuval Ebenstein
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  ABSTRACT: All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome. The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.
  Angewandte Chemie International Edition 02/2012; 51(15):3578-81. · 13.45 Impact Factor
• Article: Enzymatically Incorporated Genomic Tags for Optical Mapping of DNA-Binding Proteins.

  Soohong Kim, Anna Gottfried, Ron R Lin, Thomas Dertinger, Andrew S Kim, Sangyoon Chung, Ryan A Colyer, Elmar Weinhold, Shimon Weiss, Yuval Ebenstein
  [show abstract] [hide abstract]
  ABSTRACT: The genome of the T7 bacteriophage can be mapped by using sequence-specific methyltransferase-induced labeling of DNA. In their Communication (DOI: 10.1002/anie.201107714), E. Weinhold, S. Weiss, Y. Ebenstein, and co-workers show that the location of RNA polymerases that are bound to DNA can be visualized as a linear optical barcode, which allows structural variations in genomic DNA to be analyzed and provides an extra layer of contextual information about the genome at the single-molecule level.
  Angewandte Chemie International Edition 02/2012; · 13.45 Impact Factor
• Article: Enzymatically-incorporated genomic markers for optical mapping of DNA-binding proteins

  Soohong Kim, Anna Gottfried, Ron R. Lin, Thomas Dertinger, Andrew S. Kim, Sangyoon Chung, Ryan A. Colyer, Elmar Weinhold, Shimon Weiss, Yuval Ebenstein
  Angewandte Chemie 02/2012;