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ABSTRACT: All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome. The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.
Angewandte Chemie International Edition 02/2012; 51(15):3578-81. · 13.45 Impact Factor
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ABSTRACT: The genome of the T7 bacteriophage can be mapped by using sequence-specific methyltransferase-induced labeling of DNA. In their Communication (DOI: 10.1002/anie.201107714), E. Weinhold, S. Weiss, Y. Ebenstein, and co-workers show that the location of RNA polymerases that are bound to DNA can be visualized as a linear optical barcode, which allows structural variations in genomic DNA to be analyzed and provides an extra layer of contextual information about the genome at the single-molecule level.
Angewandte Chemie International Edition 02/2012; · 13.45 Impact Factor
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Angewandte Chemie 02/2012;