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ABSTRACT: Platycodin D (PD) is the major triterpene saponin in the root of P. grandiflorum. The aim of the present study was to evaluate the protective effects of PD on bile duct ligation (BDL)- induced cholestasis in mice. Mice were allocated to 5 groups: sham, BDL alone, and BDL with PD treatment at 1, 2, and 4 mg/kg. PD was administered to the mice for 28 consecutive days after the BDL operation. PD treatment of BDL-operated mice decreased serum alanine aminotransferase, serum aspartate aminotransferase, and total bilirubin levels by up to 37%, 31%, and 41%, respectively, in comparison with the levels in mice that underwent BDL alone. PD treatment attenuated oxidative stress, as evidenced by an increase in anti-oxidative enzyme levels glutathione and superoxide dismutase together with a decrease in lipid peroxidation and oxidative stress indices levels of malondialdehyde and nitric oxide. Histopathological studies further confirmed the protective effects of PD on cholestasisinduced hepatic injury and liver fibrosis in mice. In addition, nuclear factor-kappa B and inducible nitric oxide synthase levels significantly decreased after PD treatment, as did the levels of hepatocyte apoptosis. Taken together, these results suggest that PD treatment might be beneficial in cholestasis-induced hepatotoxicity.
Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 10/2012; · 2.99 Impact Factor
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ABSTRACT: Platycodin D (PD) is well known as a potent triterpenoid saponin having various pharmacological activities isolated from the root of Platycodon grandiflorum (Jacq.) A. DC. (Campanulaceae). We aimed to evaluate protective effect of PD on cisplatin (CDDP)-induced nephrotoxicity. Male ICR mice were allocated into five groups as follows: Negative control, CDDP alone and CDDP with PD (0.1, 1 and 5mg/kg) treated group. PD was given for three consecutive days before CDDP injection. Increased blood urea nitrogen (BUN) and creatinine (CRE) levels in CDDP alone treated mice were decreased to normal range by pretreatment with PD. It also decreased nitric oxide (NO) and lipid peroxidation with increased antioxidant enzymes such as glutathione (GSH), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in PD pretreated mice. In histopathological examination, pretreatment with PD showed ameliorated renal injury such as intraluminal cast formation and epithelial desquamation. Furthermore, over-expression of nuclear factor-kappa B p65 and apoptotic cells were suppressed by PD pretreatment. Taken together, PD pretreatment might be beneficial to CDDP-induced nephrotoxicity.
Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 05/2012; 50(12):4254-4259. · 2.99 Impact Factor
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Tae-Won Kim,
Jong-Hwan Lim,
In-Bae Song,
Sang-Jin Park,
Jae-Won Yang,
Jung Cheul Shin,
Joo-Won Suh,
Hwa-Young Son, Eun-Sang Cho,
Myoung-Seok Kim,
Sang-Wook Lee,
Jong-Woo Kim,
Hyo-In Yun
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ABSTRACT: The present study aims to evaluate the anti-HCV activity of hotwater extract from Platycodon grandiflorum (BC703) with HCV genotype 1b subgenomic replicon system and investigate its hepatoprotective activity on carbon tetrachloride (CCl(4))-induced acute liver damage in mice. BC703 produced significant hepatoprotective effects against CCl(4)-induced acute hepatic injury by decreasing the activities of serum enzymes, nitric oxide and lipid peroxidation. Histopathological studies further substantiated the protective effect of BC703. Furthermore, BC703 inhibited the HCV RNA replication with an EC(50) value and selective index (CC(50)/EC(50)) of 2.82 µg/mL and above 35.46, respectively. However, digested BC703 using a simulated gastric juice showed poor protective effect against CCl(4)-induced hepatotoxicity in mice and decreased anti-HCV activity as compared to the intact BC703. Although further studies are necessary, BC703 may be a beneficial agent for the management of acute hepatic injury and chronic HCV infection.
Journal of Nutritional Science and Vitaminology 01/2012; 58(3):187-94. · 1.20 Impact Factor
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Jong-Hwan Lim,
Tae-Won Kim,
Sang-Jin Park,
In-Bae Song,
Myoung-Seok Kim,
Hyo-Jung Kwon, Eun-Sang Cho,
Hwa-Young Son,
Sang-Wook Lee,
Joo-Won Suh,
Jong-Woo Kim,
Hyo-In Yun
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ABSTRACT: The aim of the present study was to evaluate the protective activity of aqueous extract from Platycodon grandiflorum (BC703) on thioacetamide (TA)-induced hepatotoxicity in mice. We found that BC703 significantly decreased mortality and the change in serum transaminase following TA administration. The group treated with BC703 at doses of 1, 5, and 10 mg/kg produced significant hepatoprotective effects against TA-induced liver damage by decreasing the activities of serum enzymes, nitric oxide and lipid peroxidation in dose-dependent manners. Histopathological studies further substantiated the protective effect of BC703. These results show the hepatoprotective activity of aqueous extract from Platycodon grandiflorum on thioacetamide-induced fulminant hepatic failure.
Journal of Toxicologic Pathology 12/2011; 24(4):223-8. · 0.48 Impact Factor
