Chongxi Liu

Northeast Agricultural University, Charbin, Heilongjiang Sheng, China

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Publications (29)56.52 Total impact

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    ABSTRACT: A novel actinomycete, designated strain NEAU-CY18(T), was isolated from the root of a Chinese medicinal plant Dianthus chinensis L and subjected to a polyphasic taxonomic study. The novel strain was found to develop spherical sporangia with non-motile spores on aerial mycelium. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The whole-cell sugars were identified as madurose, mannose, ribose, galactose and glucose. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were identified as MK-9(H4), MK-9(H2) and MK-9(H6). The major fatty acids were identified as C17:0 10-methyl, iso-C16:0 and C16:0. EzTaxon-e analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Sphaerisporangium and was most closely related to Sphaerisporangium cinnabarinum JCM 3291(T) (98.9 %) and Sphaerisporangium melleum JCM 13064(T) (98.3 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-CY18(T) forms a monophyletic clade with S. cinnabarinum JCM 3291(T), an association that was supported by a bootstrap value of 97 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. Comparisons of some phenotypic properties and low DNA-DNA relatedness values enabled the strain to be differentiated from S. cinnabarinum JCM 3291(T) and S. melleum JCM 13064(T). Therefore, it is concluded that strain NEAU-CY18(T) represents a novel Sphaerisporangium species, for which the name Sphaerisporangium dianthi sp. nov. is proposed. The type strain is NEAU-CY18(T) ( = CGMCC 4.7132(T) = DSM 46736(T)).
    Antonie van Leeuwenhoek 10/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-C3(T), was isolated from volcanic sediment collected from Longwan, Jilin province, north China and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the members of the genus Streptomyces. EzTaxon-e analysis of the 16S rRNA gene sequence indicated strain NEAU-C3(T) to be most closely related to Streptomyces hygroscopicus subsp. ossamyceticus JCM 4965(T) (97.7 % sequence similarity) and Streptomyces torulosus JCM 4872(T) (97.7 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-C3(T) belongs within the genus Streptomyces and forms a separate subclade, an association that was supported by a bootstrap value of 72 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The DNA-DNA hybridization values between strain NEAU-C3(T) and the two most closely related type strains were low enough to justify the assignment of the strain to a novel species. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain NEAU-C3(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces vulcanius sp. nov. is proposed. The type strain is NEAU-C3(T) (=CGMCC 4.7177(T)=DSM 42139(T)).
    Antonie van Leeuwenhoek 10/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-SH16(T) was isolated from a soil sample collected from the riverbank of Wusong river in Shanghai and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-SH16(T) were consistent with the description of the genus Catellatospora. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-SH16(T) formed a distinct branch with the closest relatives Catellatospora coxensis DSM 44901(T) (99.2 %) and Catellatospora citrea DSM 44097(T) (99.0 %), an association that was supported by a bootstrap value of 78 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA-DNA relatedness and some different physiological characteristics allowed the strain to be distinguished from its closest relatives. Thus, it is proposed that strain NEAU-SH16(T) represents a novel Catellatospora species. Catellatospora aurea sp. nov. The type strain of Catellatospora aurea is NEAU-SH16(T) (=CGMCC 4.7147(T) = DSM 46719(T)).
    Antonie van Leeuwenhoek 09/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinobacterium, designated strain NEAU-ML12(T), was isolated from a millipede (Kronopolites svenhedind Verhoeff), which was collected from Fenghuang Mountain in Wuchang, Heilongjiang Province, north China. The strain was characterized using a polyphasic approach. Strain NEAU-ML12(T) was found to have morphological and chemotaxonomic characteristics typical of the members of the genus Rhodococcus. 16S rRNA gene sequence similarity analysis showed that the strain NEAU-ML12(T) belongs to the genus Rhodococcus, and was most closely related to Rhodococcus tukisamuensis Mb8(T) (98.9 %) and Rhodococcus koreensis DNP505(T) (97.7 %). Phylogenetic analysis based on 16S rRNA gene sequences also demonstrated that strain NEAU-ML12(T) should be classified in the genus Rhodococcus, forming a distinct clade with R. tukisamuensis Mb8(T) supported by a 99 % bootstrap value. However, the DNA-DNA relatedness between strain NEAU-ML12(T) and R. tukisamuensis Mb8(T) was found to be 41.9 ± 0.7 %. Furthermore, strain NEAU-ML12(T) could also be differentiated from R. tukisamuensis Mb8(T) and other closely related strains (R. koreensis DNP505(T) and Rhodococcus maanshanensis M712(T)) by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-ML12(T) represents a novel species of the genus Rhodococcus, for which the name Rhodococcus kronopolitis sp. nov. is proposed. The type strain is NEAU-ML12(T) (=CGMCC 4.7145(T) = DSM 46702(T)).
    Antonie van Leeuwenhoek 09/2014; · 2.07 Impact Factor
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    ABSTRACT: A Gram-stain positive, filamentous bacterial strain, designated strain NEAU-TWSJ13(T), was isolated from the rhizosphere of a marigold (Tagetes erecta L.) plant collected in Heilongjiang Province, northeast China, and characterized using a polyphasic approach. The strain was observed to form abundant aerial hyphae differentiated into spherical sporangia. 16S rRNA gene sequence similarity studies showed that strain NEAU-TWSJ13(T) belongs to the genus Streptosporangium, being most closely related to Streptosporangium fragile DSM 43847(T) (98.6 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that it formed a phyletic line with S. fragile DSM 43847(T), Streptosporangium jomthongense NBRC 110047(T) (98.4 % 16S rRNA gene similarity) and Streptosporangium violaceochromogenes DSM 43849(T) (97.6 % 16S rRNA gene similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-TWSJ13(T) can be distinguished from S. fragile DSM 43847(T) and S. jomthongense NBRC 110047(T). Moreover, strain NEAU-TWSJ13(T) can also be differentiated from S. violaceochromogenes DSM 43849(T) and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-TWSJ13(T) represents a novel species of the genus Streptosporangium, for which the name Streptosporangium subfuscum sp. nov. is proposed. The type strain is NEAU-TWSJ13(T) ( = CGMCC 4.7146(T) = DSM = 46724(T)).
    Antonie van Leeuwenhoek 09/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-yn17T, was isolated from a soil sample collected at waste water discharge site of a pesticide factory in Harbin, north China and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-yn17T were consistent with the description of the genus Saccharothrix, such as the spore arrangement, the diamino acid of the peptidoglycan, the whole-cell hydrolysates, the predominant menaquinone and the phospholipid profile. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-yn17T should also be classified in the genus Saccharothrix, with the type strains of Saccharothrix saharensis DSM 45456T (99.52 % sequence similarity) and Saccharothrix xinjiangensis JCM 12329T (99.04 %) as the nearest phylogenetic relatives. A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-yn17T can be distinguished from its closest relatives. Therefore, strain NEAU-yn17T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix carnea sp. nov. is proposed. The type strain is NEAU-yn17T (=CGMCC 4.7097T=DSM 45878T).Therefore, it is proposed that strain NEAU-yn17T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix carnea sp. nov. is proposed. The type strain is NEAU-yn17T (=CGMCC 4.7097T=DSM 45878T).
    International journal of systematic and evolutionary microbiology. 09/2014;
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    ABSTRACT: A novel actinomycete, designated strain NEAU-P5(T), was isolated from dandelion root (Taraxacum mongolicum Hand.-Mazz.). Strain NEAU-P5(T) showed closest 16S rRNA gene sequence similarity to Micromonospora chokoriensis 2-19/6(T) (99.5 %), and phylogenetically clustered with Micromonospora violae NEAU-zh8(T) (99.3 %), M. saelicesensis Lupac 09(T) (99.0 %), M. lupini Lupac 14N(T) (98.8 %), M. zeae NEAU-gq9(T) (98.4 %), M. jinlongensis NEAU-GRX11(T) (98.3 %) and M. zamorensis CR38(T) (97.9 %). Phylogenetic analysis based on the gyrB gene sequence also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8(T). The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-9(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C16:0, iso-C15:0 and C17:0. Furthermore, some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-P5(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora taraxaci sp. nov. is proposed. The type strain is NEAU-P5(T) (=CGMCC 4.7098(T) = DSM 45885(T)).
    Antonie van Leeuwenhoek 08/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-gq9(T), was isolated from corn root (Zea mays L.) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-gq9(T) was most closely related to Micromonospora zamorensis CR38(T) (99.3%), Micromonospora jinlongensis NEAU-GRX11(T) (99.2%), Micromonospora saelicesensis Lupac 09(T) (99.2%), Micromonospora chokoriensis 2-19(6)(T) (98.9%), Micromonospora coxensis 2-30-b(28)(T) (98.6%) and Micromonospora lupini Lupac 14N(T) (98.5%). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-gq9(T) is a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38(T), M. jinlongensis NEAU-GRX11(T), M. saelicesensis Lupac 09(T), M. chokoriensis 2-19(6)(T) and M. lupini Lupac 14N(T). A combination of DNA-DNA hybridization, morphological and physiological characteristics indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that strain NEAU-gq9(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora zeae sp. nov. is proposed. The type strain is NEAU-gq9(T) (=CGMCC 4.7092(T)=DSM 45882(T)).The Journal of Antibiotics advance online publication, 21 May 2014; doi:10.1038/ja.2014.54.
    The Journal of antibiotics. 05/2014;
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    ABSTRACT: A novel actinomycete, designated strain NEAU-zh8(T), was isolated from a root of Viola philippica Car collected in China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-zh8(T) belongs to the genus Micromonospora, being most closely related to Micromonospora chokoriensis 2-9(6)(T) (99.9 %), Micromonospora saelicesensis Lupac 09(T) (99.3 %) and Micromonospora lupini Lupac 14N(T) (99.0 %). gyrB gene analysis also indicated that strain NEAU-zh8(T) should be assigned to the genus Micromonospora. The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-10(H4), MK-10(H2) and MK-10(H6). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were iso-C15:0, C16:0 and C17:0 10-methyl. A combination of DNA-DNA hybridization results and some physiological and biochemical properties indicated that strain NEAU-zh8(T) could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that strain NEAU-zh8(T) represents a novel Micromonospora species, for which the name Micromonospora violae sp. nov. is proposed. The type strain is NEAU-zh8(T) (=CGMCC 4.7102(T)=DSM 45888(T)).
    Antonie van Leeuwenhoek 05/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-NH11(T), was isolated from muddy soil collected from a lake and characterized using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-NH11(T) belongs to the genus Streptosporangium, and was most closely related to Streptosporangium amethystogenes subsp. amethystogenes DSM 43179(T) (99.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-NH11(T) formed a monophyletic clade with Streptosporangium purpuratum CY-15110(T) (98.3 %) and Streptosporangium yunnanense CY-11007(T) (98.0 %), an association that was supported by a bootstrap value of 80 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from S. amethystogenes subsp. amethystogenes DSM 43179(T), S. purpuratum CY-15110(T) and S. yunnanense CY-11007(T). Moreover, strain NEAU-NH11(T) could also be differentiated from its closest related strains by phenotypic characteristics. Therefore, it is proposed that strain NEAU-NH11(T) represents a novel Streptosporangium species, Streptosporangium nanhuense sp. nov. The type strain of S. nanhuense is NEAU-NH11(T). (=CGMCC 4.7131(T) = DSM 46674(T)).
    Antonie van Leeuwenhoek 04/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-st4(T), was isolated from a soil sample collected from Shaanxi province, Northwest China and characterized using a polyphasic approach. 16S rRNA gene sequence analysis revealed that strain NEAU-st4(T) has the highest sequence similarities with Actinomycetospora rishiriensis RI109-Li102(T) (99.4 %), Actinomycetospora corticicola 014-5(T) (99.1 %), Actinomycetospora chiangmaiensis YIM 0006(T) (98.8 %) and Actinomycetospora iriomotensis IR73-Li102(T) (98.2 %). However, the low level of DNA-DNA relatedness differentiated strain NEAU-st4(T) from its closest phylogenetic neighbours. The main chemotaxonomic properties of strain NEAU-st4(T), such as the diamino acid of the peptidoglycan, the whole-cell hydrolysates, the predominant menaquinones and the phospholipid profile, supported its classification within the genus Actinomycetospora. The distinctive morphology of this strain compared with that of other members in the genus Actinomycetospora is the formation of sporangia directly on the substrate hyphae. Phenotypic and genotypic differences also allowed the distinction of the strain from closely related species. Consequently, strain NEAU-st4(T) represents a new species of the genus Actinomycetospora, for which the name Actinomycetospora atypica sp. nov. is proposed. The type strain is NEAU-st4(T) (=CGMCC 4.7093(T) = DSM 45873(T)).
    Antonie van Leeuwenhoek 02/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-GRX6T, was isolated from mucky soil collected from a stream of Jinlong Mountain in Harbin, Heilongjiang Province, north China, and characterized using a polyphasic approach. The isolate formed irregular sporangia containing motile sporangiospores on substrate mycelia. The whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-9(H6), MK-10(H4) and MK-9(H4). The major fatty acids were C16:0, C15:0, C18:1 ω9c, C17:1 ω7c and C18:0. The phospholipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidyglycerol (PG) and phosphatidylinositol (PI). The DNA G+C content was 67 mol%. 16S rRNA gene sequence similarity studies showed that strain NEAU-GRX6T belonged to the genus Actinoplanes, being most closely related to Actinoplanes palleronii IFO 14916T (97.80%) and Actinoplanes missouriensis NBRC 102363T (97.76%). However, the low level of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned Actinoplanes species. Moreover, strain NEAU-GRX6T could also be distinguished from A. palleronii IFO 14916T and A. missouriensis NBRC 102363T by phenotypic characteristics. Therefore, it is proposed that strain NEAU-GRX6T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes lutulentus sp. nov. is proposed. The type strain of Actinoplanes lutulentus is strain NEAU-GRX6T (=CGMCC 4.7090T=DSM 45883T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-ycm1T, was isolated from an edible Chinese black ant (Polyrhachis vicina Roger) and characterized with a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence show that the novel isolate belongs to the genus Streptomyces and forms a separate subclade. The closest phylogenetic relatives were identified as the type strains of Streptomyces intermedius NBRC 13049T (97.74 %), Streptomyces aureoverticillatus NRRL B-3326T (97.69 %), Streptomyces rutgersensis NBRC 12819T (97.68 %), Streptomyces gougerotii NBRC 3198T (97.68 %) and Streptomyces diastaticus subsp. diastaticus NBRC 3714T (97.68 %). Similarities to other type strains of the genus Streptomyces were lower than 97.55 %. A comparison between strain NEAU-ycm1T and the closest related Streptomyces type strains revealed that it is different from them in morphological, physiological and biochemical characteristics. Therefore, it is proposed that NEAU-ycm1T (=CGMCC 4.7094T = DSM 42102T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces polyrhachii sp. nov. is proposed.
    Antonie van Leeuwenhoek 02/2014; · 2.07 Impact Factor
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    ABSTRACT: Borrelidin exhibits a wide spectrum of biological activities and has been considered as a non-competitive inhibitor of Threonyl-tRNA synthetase (ThrRS). However, the detailed mechanisms of borrelidin against ThrRS, especially borrelidin binding site on ThrRS, are still unclear, which limits the development of novel borrelidin derivatives and rational design of structure-based ThrRS inhibitors. In this study, the binding site of borrelidin on E. coli ThrRS was predicted by molecular docking. To validate our speculations, the ThrRS mutants of E. coli (P424K, E458Δ, and G459Δ) were constructed and their sensitivity to borrelidin was compared to that of the wild-type ThrRS by enzyme kinetics and stopped-flow fluorescence analysis. The docking results showed that borrelidin binds the pocket outside but adjacent to the active site of ThrRS, consisting of residue Y313, R363, R375, P424, E458, G459, and K465. Site-directed mutagenesis results showed that sensitivities of P424K, E458Δ, and G459Δ ThrRSs to borrelidin were reduced markedly. All the results showed that residue Y313, P424, E458, and G459 play vital roles in the binding of borrelidin to ThrRS. It indicated that borrelidin may induce the cleft closure, which blocks the release of Thr-AMP and PPi, to inhibit activity of ThrRS rather than inhibit the binding of ATP and threonine. This study provides new insight into inhibitory mechanisms of borrelidin against ThrRS, and paves the way for development of novel borrelidin derivatives and rational design of structure-based ThrRS inhibitors.
    Biochemical and Biophysical Research Communications 01/2014; · 2.28 Impact Factor
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    ABSTRACT: A novel endophytic actinomycete, designated strain NEAU-LZS-5T, was isolated from the leaf of candelabraaloe (Aloe arborescens Mill) and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain NEAU-LZS-5T belongs to the genus Streptomyces and exhibited 99.51% and 97.37% gene sequence similarities to Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T, respectively, whereas low sequence similarity values (< 97%) distinguished strain NEAU-LZS-5T from all other Streptomyces species with validly published names. Two tree-making algorithms also supported the position that strain NEAU-LZS-5T formed a distinct clade with S. sedi YIM 65188T and S. specialis GW41-1564T. However, the DNA-DNA relatedness between strain NEAU-LZS-5T and S. sedi YIM 65188T and S. specialis GW41-1564T were found to be 45.59% and 31.9%, respectively. A comparative study between strain NEAU-LZS-5T and the closest related Streptomyces type strains revealed that it differed from them in morphological, physiological and biochemical characteristics. Therefore, strain NEAU-LZS-5T represents a novel Streptomyces species, for which the name Streptomyces zhaozhouensis sp. nov. is proposed. The type strain of Streptomyces zhaozhouensis is NEAU-LZS-5T (=CGMCC 4.7095T =DSM 42101T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2013; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-dht8T, was isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of Nonomuraea. The G+C content of the DNA was 68.47 mol%. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-dht8T was most closely related to Nonomuraea maheshkhaliensis 16-5-14T (99.31%), Nonomuraea kuesteri GW 14-1925T (98.77%), Nonomuraea coxensis JCM 13931T (98.71%), Nonomuraea wenchangensis 210417T (98.44%), Nonomuraea bangladeshensis 5-10-10T (98.36%) and Nonomuraea salmonea DSM 43678T (98.0%); similarities to other species of the genus Nonomuraea were lower than 98%. Two tree-making algorithms based on 16S rRNA gene sequences showed that the isolate formed a phyletic line with its closest neighbour N. maheshkhaliensis 16-5-14T. However, the low level of DNA-DNA relatedness allowed the isolate to be differentiated from it. Strain NEAU-dht8T could also be differentiated from other Nonomuraea species showing high 16S rRNA gene sequence similarity (98-98.77%) by morphological and physiological characteristics. Thus, strain NEAU-dht8T is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea fuscirosea sp. nov. is proposed. The type strain is NEAU-dht8T (=CGMCC 4.7104T=DSM 45880T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2013; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-MES19(T), was isolated from pine forest soil in Heilongjiang province, China. A polyphasic study was carried out to establish the taxonomic position of this strain. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain NEAU-MES19(T) was most closely related to Micromonospora matsumotoense IMSNU 22003(T). However, phylogenetic analysis based on the gyrB gene sequence showed that the isolate was more closely related to Micromonospora cremea CR30(T) than M. matsumotoense IMSNU 22003(T). The low level of DNA-DNA relatedness allowed the isolate to be differentiated from M. matsumotoense IMSNU 22003(T) and M. cremea CR30(T). Moreover, strain NEAU-MES19(T) could also be distinguished from its closest phylogenetic relatives by morphological, physiological and biochemical characteristics. Therefore, it is proposed that strain NEAU-MES19(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora maoerensis sp. nov. is proposed. The type strain is NEAU-MES19(T) (=CGMCC 4.7091(T) = DSM 45884(T)).
    Antonie van Leeuwenhoek 12/2013; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-GRX11(T), was isolated from muddy soil collected from a stream of Jinlong Mountain in Harbin, north China. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-GRX11(T) showed highest similarity to Micromonospora zamorensis CR38(T) (99.2 %), Micromonospora saelicesensis Lupac 09(T) (99.0 %), Micromonospora chokoriensis 2-19/6(T) (98.7 %), Micromonospora coxensis 2-30-b/28(T) (98.5 %), Micromonospora aurantiaca ATCC 27029(T) (98.4 %) and Micromonospora lupini lupac 14N(T) (98.3 %). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-GRX11(T) was a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38(T), M. saelicesensis Lupac 09(T), M. chokoriensis 2-19/6(T) and M. lupini lupac 14N(T). A combination of DNA-DNA hybridization and some phenotypic characteristics indicated that the novel strain could be readily distinguished from these closest phylogenetic relatives. Therefore, it is proposed that NEAU-GRX11(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora jinlongensis sp. nov. is proposed. The type strain is NEAU-GRX11(T) (=CGMCC 4.7103(T)=DSM 45876(T)).
    Antonie van Leeuwenhoek 11/2013; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-GH7(T), was isolated from a lake sediment and characterized using a polyphasic approach. Strain NEAU-GH7(T) was Gram-stain positive, aerobic, non-spore-forming and produced spherical sporangia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-GH7(T) formed a monophyletic clade with the closest relative Streptosporangium longisporum DSM 43180(T) (99.0 %), an association that was supported by a bootstrap value of 74 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from its closest relative. Moreover, strain NEAU-GH7(T) could also be differentiated from S. longisporum DSM 43180(T) and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotypic characteristics, strain NEAU-GH7(T) should be classified as a new species of the genus Streptosporangium, for which the name Streptosporangium shengliensis sp. nov. is proposed. The type strain is NEAU-GH7(T) (=CGMCC 4.7105(T)=DSM 45881(T)).
    Antonie van Leeuwenhoek 11/2013; · 2.07 Impact Factor
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    ABSTRACT: Two novel actinomycetes, designated strains NEAU-ZJ3(T) and NEAU-yn31(T), were isolated from soils in China and their taxonomic positions determined by a polyphasic approach. Key morphological and chemotaxonomic properties of the two strains were consistent with the description of the genus Nonomuraea. The G+C contents of strains NEAU-ZJ3(T) and NEAU-yn31(T) were determined to be 67.4 and 68.0 %, respectively. Phylogenetic analysis using 16S rRNA gene sequences indicated that the two strains should be classified in the genus Nonomuraea. The 16S rRNA gene sequence of strain NEAU-ZJ3(T) showed it to be most closely related to Nonomuraea salmonea DSM 43678(T) (98.27 %), Nonomuraea endophytica YIM 65601(T) (98.23 %) and Nonomuraea candida HMC10(T) (98.08 %), and that of strain NEAU-yn31(T) to Nonomuraea ferruginea IFO 14094(T) (99.08 %) and N. candida HMC10(T) (98.01 %). Furthermore, a combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that the two strains can be distinguished from each other and their phylogenetically closest relatives. Thus, strains NEAU-ZJ3(T) and NEAU-yn31(T) represent two novel species of the genus Nonomuraea, for which the names Nonomuraea guangzhouensis sp. nov. and Nonomuraea harbinensis sp. nov. are proposed. The type strains are NEAU-ZJ3(T) (=CGMCC 4.7101(T) = DSM 45889(T)) and NEAU-yn31(T) (=CGMCC 4.7106(T) = DSM 45887(T)), respectively.
    Antonie van Leeuwenhoek 11/2013; · 2.07 Impact Factor