Chongxi Liu

Northeast Agricultural University, Charbin, Heilongjiang Sheng, China

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Publications (23)46.29 Total impact

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    ABSTRACT: A novel actinomycete, designated strain NEAU-P5(T), was isolated from dandelion root (Taraxacum mongolicum Hand.-Mazz.). Strain NEAU-P5(T) showed closest 16S rRNA gene sequence similarity to Micromonospora chokoriensis 2-19/6(T) (99.5 %), and phylogenetically clustered with Micromonospora violae NEAU-zh8(T) (99.3 %), M. saelicesensis Lupac 09(T) (99.0 %), M. lupini Lupac 14N(T) (98.8 %), M. zeae NEAU-gq9(T) (98.4 %), M. jinlongensis NEAU-GRX11(T) (98.3 %) and M. zamorensis CR38(T) (97.9 %). Phylogenetic analysis based on the gyrB gene sequence also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8(T). The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-9(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C16:0, iso-C15:0 and C17:0. Furthermore, some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-P5(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora taraxaci sp. nov. is proposed. The type strain is NEAU-P5(T) (=CGMCC 4.7098(T) = DSM 45885(T)).
    Antonie van Leeuwenhoek 08/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-gq9(T), was isolated from corn root (Zea mays L.) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-gq9(T) was most closely related to Micromonospora zamorensis CR38(T) (99.3%), Micromonospora jinlongensis NEAU-GRX11(T) (99.2%), Micromonospora saelicesensis Lupac 09(T) (99.2%), Micromonospora chokoriensis 2-19(6)(T) (98.9%), Micromonospora coxensis 2-30-b(28)(T) (98.6%) and Micromonospora lupini Lupac 14N(T) (98.5%). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-gq9(T) is a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38(T), M. jinlongensis NEAU-GRX11(T), M. saelicesensis Lupac 09(T), M. chokoriensis 2-19(6)(T) and M. lupini Lupac 14N(T). A combination of DNA-DNA hybridization, morphological and physiological characteristics indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that strain NEAU-gq9(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora zeae sp. nov. is proposed. The type strain is NEAU-gq9(T) (=CGMCC 4.7092(T)=DSM 45882(T)).The Journal of Antibiotics advance online publication, 21 May 2014; doi:10.1038/ja.2014.54.
    The Journal of antibiotics. 05/2014;
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    ABSTRACT: A novel actinomycete, designated strain NEAU-zh8(T), was isolated from a root of Viola philippica Car collected in China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-zh8(T) belongs to the genus Micromonospora, being most closely related to Micromonospora chokoriensis 2-9(6)(T) (99.9 %), Micromonospora saelicesensis Lupac 09(T) (99.3 %) and Micromonospora lupini Lupac 14N(T) (99.0 %). gyrB gene analysis also indicated that strain NEAU-zh8(T) should be assigned to the genus Micromonospora. The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-10(H4), MK-10(H2) and MK-10(H6). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were iso-C15:0, C16:0 and C17:0 10-methyl. A combination of DNA-DNA hybridization results and some physiological and biochemical properties indicated that strain NEAU-zh8(T) could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that strain NEAU-zh8(T) represents a novel Micromonospora species, for which the name Micromonospora violae sp. nov. is proposed. The type strain is NEAU-zh8(T) (=CGMCC 4.7102(T)=DSM 45888(T)).
    Antonie van Leeuwenhoek 05/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-NH11(T), was isolated from muddy soil collected from a lake and characterized using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-NH11(T) belongs to the genus Streptosporangium, and was most closely related to Streptosporangium amethystogenes subsp. amethystogenes DSM 43179(T) (99.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-NH11(T) formed a monophyletic clade with Streptosporangium purpuratum CY-15110(T) (98.3 %) and Streptosporangium yunnanense CY-11007(T) (98.0 %), an association that was supported by a bootstrap value of 80 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from S. amethystogenes subsp. amethystogenes DSM 43179(T), S. purpuratum CY-15110(T) and S. yunnanense CY-11007(T). Moreover, strain NEAU-NH11(T) could also be differentiated from its closest related strains by phenotypic characteristics. Therefore, it is proposed that strain NEAU-NH11(T) represents a novel Streptosporangium species, Streptosporangium nanhuense sp. nov. The type strain of S. nanhuense is NEAU-NH11(T). (=CGMCC 4.7131(T) = DSM 46674(T)).
    Antonie van Leeuwenhoek 04/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-st4(T), was isolated from a soil sample collected from Shaanxi province, Northwest China and characterized using a polyphasic approach. 16S rRNA gene sequence analysis revealed that strain NEAU-st4(T) has the highest sequence similarities with Actinomycetospora rishiriensis RI109-Li102(T) (99.4 %), Actinomycetospora corticicola 014-5(T) (99.1 %), Actinomycetospora chiangmaiensis YIM 0006(T) (98.8 %) and Actinomycetospora iriomotensis IR73-Li102(T) (98.2 %). However, the low level of DNA-DNA relatedness differentiated strain NEAU-st4(T) from its closest phylogenetic neighbours. The main chemotaxonomic properties of strain NEAU-st4(T), such as the diamino acid of the peptidoglycan, the whole-cell hydrolysates, the predominant menaquinones and the phospholipid profile, supported its classification within the genus Actinomycetospora. The distinctive morphology of this strain compared with that of other members in the genus Actinomycetospora is the formation of sporangia directly on the substrate hyphae. Phenotypic and genotypic differences also allowed the distinction of the strain from closely related species. Consequently, strain NEAU-st4(T) represents a new species of the genus Actinomycetospora, for which the name Actinomycetospora atypica sp. nov. is proposed. The type strain is NEAU-st4(T) (=CGMCC 4.7093(T) = DSM 45873(T)).
    Antonie van Leeuwenhoek 02/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-GRX6T, was isolated from mucky soil collected from a stream of Jinlong Mountain in Harbin, Heilongjiang Province, north China, and characterized using a polyphasic approach. The isolate formed irregular sporangia containing motile sporangiospores on substrate mycelia. The whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-9(H6), MK-10(H4) and MK-9(H4). The major fatty acids were C16:0, C15:0, C18:1 ω9c, C17:1 ω7c and C18:0. The phospholipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidyglycerol (PG) and phosphatidylinositol (PI). The DNA G+C content was 67 mol%. 16S rRNA gene sequence similarity studies showed that strain NEAU-GRX6T belonged to the genus Actinoplanes, being most closely related to Actinoplanes palleronii IFO 14916T (97.80%) and Actinoplanes missouriensis NBRC 102363T (97.76%). However, the low level of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned Actinoplanes species. Moreover, strain NEAU-GRX6T could also be distinguished from A. palleronii IFO 14916T and A. missouriensis NBRC 102363T by phenotypic characteristics. Therefore, it is proposed that strain NEAU-GRX6T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes lutulentus sp. nov. is proposed. The type strain of Actinoplanes lutulentus is strain NEAU-GRX6T (=CGMCC 4.7090T=DSM 45883T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-ycm1T, was isolated from an edible Chinese black ant (Polyrhachis vicina Roger) and characterized with a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence show that the novel isolate belongs to the genus Streptomyces and forms a separate subclade. The closest phylogenetic relatives were identified as the type strains of Streptomyces intermedius NBRC 13049T (97.74 %), Streptomyces aureoverticillatus NRRL B-3326T (97.69 %), Streptomyces rutgersensis NBRC 12819T (97.68 %), Streptomyces gougerotii NBRC 3198T (97.68 %) and Streptomyces diastaticus subsp. diastaticus NBRC 3714T (97.68 %). Similarities to other type strains of the genus Streptomyces were lower than 97.55 %. A comparison between strain NEAU-ycm1T and the closest related Streptomyces type strains revealed that it is different from them in morphological, physiological and biochemical characteristics. Therefore, it is proposed that NEAU-ycm1T (=CGMCC 4.7094T = DSM 42102T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces polyrhachii sp. nov. is proposed.
    Antonie van Leeuwenhoek 02/2014; · 2.07 Impact Factor
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    ABSTRACT: Borrelidin exhibits a wide spectrum of biological activities and has been considered as a non-competitive inhibitor of Threonyl-tRNA synthetase (ThrRS). However, the detailed mechanisms of borrelidin against ThrRS, especially borrelidin binding site on ThrRS, are still unclear, which limits the development of novel borrelidin derivatives and rational design of structure-based ThrRS inhibitors. In this study, the binding site of borrelidin on E. coli ThrRS was predicted by molecular docking. To validate our speculations, the ThrRS mutants of E. coli (P424K, E458Δ, and G459Δ) were constructed and their sensitivity to borrelidin was compared to that of the wild-type ThrRS by enzyme kinetics and stopped-flow fluorescence analysis. The docking results showed that borrelidin binds the pocket outside but adjacent to the active site of ThrRS, consisting of residue Y313, R363, R375, P424, E458, G459, and K465. Site-directed mutagenesis results showed that sensitivities of P424K, E458Δ, and G459Δ ThrRSs to borrelidin were reduced markedly. All the results showed that residue Y313, P424, E458, and G459 play vital roles in the binding of borrelidin to ThrRS. It indicated that borrelidin may induce the cleft closure, which blocks the release of Thr-AMP and PPi, to inhibit activity of ThrRS rather than inhibit the binding of ATP and threonine. This study provides new insight into inhibitory mechanisms of borrelidin against ThrRS, and paves the way for development of novel borrelidin derivatives and rational design of structure-based ThrRS inhibitors.
    Biochemical and Biophysical Research Communications 01/2014; · 2.41 Impact Factor
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    ABSTRACT: A novel endophytic actinomycete, designated strain NEAU-LZS-5T, was isolated from the leaf of candelabraaloe (Aloe arborescens Mill) and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain NEAU-LZS-5T belongs to the genus Streptomyces and exhibited 99.51% and 97.37% gene sequence similarities to Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T, respectively, whereas low sequence similarity values (< 97%) distinguished strain NEAU-LZS-5T from all other Streptomyces species with validly published names. Two tree-making algorithms also supported the position that strain NEAU-LZS-5T formed a distinct clade with S. sedi YIM 65188T and S. specialis GW41-1564T. However, the DNA-DNA relatedness between strain NEAU-LZS-5T and S. sedi YIM 65188T and S. specialis GW41-1564T were found to be 45.59% and 31.9%, respectively. A comparative study between strain NEAU-LZS-5T and the closest related Streptomyces type strains revealed that it differed from them in morphological, physiological and biochemical characteristics. Therefore, strain NEAU-LZS-5T represents a novel Streptomyces species, for which the name Streptomyces zhaozhouensis sp. nov. is proposed. The type strain of Streptomyces zhaozhouensis is NEAU-LZS-5T (=CGMCC 4.7095T =DSM 42101T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2013; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-dht8T, was isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of Nonomuraea. The G+C content of the DNA was 68.47 mol%. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-dht8T was most closely related to Nonomuraea maheshkhaliensis 16-5-14T (99.31%), Nonomuraea kuesteri GW 14-1925T (98.77%), Nonomuraea coxensis JCM 13931T (98.71%), Nonomuraea wenchangensis 210417T (98.44%), Nonomuraea bangladeshensis 5-10-10T (98.36%) and Nonomuraea salmonea DSM 43678T (98.0%); similarities to other species of the genus Nonomuraea were lower than 98%. Two tree-making algorithms based on 16S rRNA gene sequences showed that the isolate formed a phyletic line with its closest neighbour N. maheshkhaliensis 16-5-14T. However, the low level of DNA-DNA relatedness allowed the isolate to be differentiated from it. Strain NEAU-dht8T could also be differentiated from other Nonomuraea species showing high 16S rRNA gene sequence similarity (98-98.77%) by morphological and physiological characteristics. Thus, strain NEAU-dht8T is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea fuscirosea sp. nov. is proposed. The type strain is NEAU-dht8T (=CGMCC 4.7104T=DSM 45880T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2013; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-MES19(T), was isolated from pine forest soil in Heilongjiang province, China. A polyphasic study was carried out to establish the taxonomic position of this strain. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain NEAU-MES19(T) was most closely related to Micromonospora matsumotoense IMSNU 22003(T). However, phylogenetic analysis based on the gyrB gene sequence showed that the isolate was more closely related to Micromonospora cremea CR30(T) than M. matsumotoense IMSNU 22003(T). The low level of DNA-DNA relatedness allowed the isolate to be differentiated from M. matsumotoense IMSNU 22003(T) and M. cremea CR30(T). Moreover, strain NEAU-MES19(T) could also be distinguished from its closest phylogenetic relatives by morphological, physiological and biochemical characteristics. Therefore, it is proposed that strain NEAU-MES19(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora maoerensis sp. nov. is proposed. The type strain is NEAU-MES19(T) (=CGMCC 4.7091(T) = DSM 45884(T)).
    Antonie van Leeuwenhoek 12/2013; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-GRX11(T), was isolated from muddy soil collected from a stream of Jinlong Mountain in Harbin, north China. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-GRX11(T) showed highest similarity to Micromonospora zamorensis CR38(T) (99.2 %), Micromonospora saelicesensis Lupac 09(T) (99.0 %), Micromonospora chokoriensis 2-19/6(T) (98.7 %), Micromonospora coxensis 2-30-b/28(T) (98.5 %), Micromonospora aurantiaca ATCC 27029(T) (98.4 %) and Micromonospora lupini lupac 14N(T) (98.3 %). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-GRX11(T) was a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38(T), M. saelicesensis Lupac 09(T), M. chokoriensis 2-19/6(T) and M. lupini lupac 14N(T). A combination of DNA-DNA hybridization and some phenotypic characteristics indicated that the novel strain could be readily distinguished from these closest phylogenetic relatives. Therefore, it is proposed that NEAU-GRX11(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora jinlongensis sp. nov. is proposed. The type strain is NEAU-GRX11(T) (=CGMCC 4.7103(T)=DSM 45876(T)).
    Antonie van Leeuwenhoek 11/2013; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-GH7(T), was isolated from a lake sediment and characterized using a polyphasic approach. Strain NEAU-GH7(T) was Gram-stain positive, aerobic, non-spore-forming and produced spherical sporangia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-GH7(T) formed a monophyletic clade with the closest relative Streptosporangium longisporum DSM 43180(T) (99.0 %), an association that was supported by a bootstrap value of 74 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from its closest relative. Moreover, strain NEAU-GH7(T) could also be differentiated from S. longisporum DSM 43180(T) and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotypic characteristics, strain NEAU-GH7(T) should be classified as a new species of the genus Streptosporangium, for which the name Streptosporangium shengliensis sp. nov. is proposed. The type strain is NEAU-GH7(T) (=CGMCC 4.7105(T)=DSM 45881(T)).
    Antonie van Leeuwenhoek 11/2013; · 2.07 Impact Factor
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    ABSTRACT: Two novel actinomycetes, designated strains NEAU-ZJ3(T) and NEAU-yn31(T), were isolated from soils in China and their taxonomic positions determined by a polyphasic approach. Key morphological and chemotaxonomic properties of the two strains were consistent with the description of the genus Nonomuraea. The G+C contents of strains NEAU-ZJ3(T) and NEAU-yn31(T) were determined to be 67.4 and 68.0 %, respectively. Phylogenetic analysis using 16S rRNA gene sequences indicated that the two strains should be classified in the genus Nonomuraea. The 16S rRNA gene sequence of strain NEAU-ZJ3(T) showed it to be most closely related to Nonomuraea salmonea DSM 43678(T) (98.27 %), Nonomuraea endophytica YIM 65601(T) (98.23 %) and Nonomuraea candida HMC10(T) (98.08 %), and that of strain NEAU-yn31(T) to Nonomuraea ferruginea IFO 14094(T) (99.08 %) and N. candida HMC10(T) (98.01 %). Furthermore, a combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that the two strains can be distinguished from each other and their phylogenetically closest relatives. Thus, strains NEAU-ZJ3(T) and NEAU-yn31(T) represent two novel species of the genus Nonomuraea, for which the names Nonomuraea guangzhouensis sp. nov. and Nonomuraea harbinensis sp. nov. are proposed. The type strains are NEAU-ZJ3(T) (=CGMCC 4.7101(T) = DSM 45889(T)) and NEAU-yn31(T) (=CGMCC 4.7106(T) = DSM 45887(T)), respectively.
    Antonie van Leeuwenhoek 11/2013; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, strain NEAU-st1(T), was isolated from a soil sample collected in Shaanxi province, Northwest China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-st1(T) belongs to the genus Nonomuraea, being most closely related to Nonomuraea rosea GW12687(T) (98.91 %), Nonomuraea solani NEAU-Z6(T) (98.44 %), Nonomuraea rhizophila YIM67092(T)(98.24 %) and Nonomuraea monospora PT708(T) (98.02 %); similarities to sequences of other type strains of the genus Nonomuraea were lower than 98 %. Both tree-making algorithms used also supported the position that strain NEAU-st1(T) formed a distinct clade with its most closely related species. Morphological and physiological characteristics confirmed that the strain belongs to the genus Nonomuraea and distinguished it from its most closely related species. DNA-DNA hybridization further differentiated strain NEAU-st1(T) from its nearest phylogenetic neighbours. These results suggested that strain NEAU-st1(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea shaanxiensis sp. nov. is proposed. The type strain is NEAU-st1(T) (=CGMCC 4.7096(T) = DSM 45877(T)).
    Antonie van Leeuwenhoek 10/2013; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-ycm2T, was isolated from edible Chinese black ant (Polyrhachis vicina Roger) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-ycm2T showed highest similarity to Micromonospora sonneratiae 274745T (99.12%), Micromonospora pattaloongensis TJ2-2T (98.85%), Micromonospora pisi GUI 15T (98.76%), Polymorphospora rubra TT 97-42T (98.42%) and Micromonospora eburnea LK2-10T (98.21%). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-ycm2T is a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. sonneratiae 274745T, M. pattaloongensis JCM 12833T and M. pisi GUI 15T. Furthermore, a combination of DNA-DNA hybridization and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that NEAU-ycm2T represents a novel species of the genus of Micromonospora, for which the name Micromonospora polyrhachis sp. nov. The type strain is NEAU-ycm2T (=CGMCC 4.7100T=DSM 45886T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2013; · 2.11 Impact Factor
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    ABSTRACT: A novel ikarugamycin-producing actinomycete, designated strain NEAU-Da3T was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-Da3T belonged to the genus Streptomyces, being most closely related to Streptomyces carpaticus NRRL B-16359T (99.5%), Streptomyces cheonanensis VC-A46T (99.3%) and Streptomyces xiamenensis MCCC 1A01550T (97.2%), similarities to other type strains of the genus Streptomyces were lower than 97.1%. The maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with S. carpaticus NRRL B-16359T, S. cheonanensis VC-A46T and S. xiamenensis MCCC 1A01550T. This branching pattern was also supported by the tree constructed with the neighbour-joining method. A comparative study between strain NEAU-Da3T and the closest related Streptomyces type strains revealed that it differed from them in morphological, physiological and biochemical characteristics. Therefore, it is proposed that strain NEAU-Da3T represents a novel Streptomyces species, Streptomyces harbinensis sp. nov. The type strain of Streptomyces harbinensis is NEAU-Da3T (=CGMCC 4.7047T=DSM 42076T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2013; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-Z6(T), was isolated from eggplant root (Solanum melongena L.). Phylogenetic analysis based on the 16S rRNA gene showed that strain NEAU-Z6(T) belonged to the genus Nonomuraea, with highest sequence similarity to Nonomuraea monospora PT 708(T) (98.83%), Nonomuraea rosea GW 12687(T) (98.55%) and Nonomuraea rhizophila YIM 67092(T) (98.02%). Sequence similarities between strain NEAU-Z6(T) and other species of the genus Nonomuraea ranged from 97.94% (Nonomuraea candida HMC10(T)) to 96.30% (Nonomuraea wenchangensis 210417(T)). Key morphological, physiological and chemotaxonomic characteristics of strain NEAU-Z6(T) were congruent with the description of the genus Nonomuraea. The G+C content of the genomic DNA was 64.51 mol%. Comparative analysis of physiological, biochemical and chemotaxonomic data as well as fatty acid profiles allowed genotypic and phenotypic differentiation of strain NEAU-Z6(T) from closely related species. Thus, strain NEAU-Z6(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea solani sp. nov. is proposed. The type strain is NEAU-Z6(T) (=CGMCC 4.07037(T)=DSM 45729(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2012; · 2.11 Impact Factor
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    ABSTRACT: A novel actinomycete, designated strain NEAU-M9(T), was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-M9(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes campanulatus DSM 43148(T) (98.85 %), Actinoplanes capillaceus DSM 44859(T) (98.70 %), Actinoplanes lobatus DSM 43150(T) (98.30 %), Actinoplanes auranticolor DSM 43031(T) (98.23 %) and Actinoplanes sichuanensis 03-723(T) (98.06 %); similarity to other type strains of the genus Actinoplanes ranged from 95.87 to 97.56 %. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with A. campanulatus DSM 43148(T) and A. capillaceus DSM 44859(T). This branching pattern was also supported by the tree constructed with the maximum-likelihood method. However, the low level of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned two Actinoplanes species. Moreover, strain NEAU-M9(T) could also be distinguished from the most closely related species by morphological, physiological and characteristics. Therefore, it is proposed that strain NEAU-M9(T) represents a novel Actinoplanes species, Actinoplanes hulinensis sp. nov. The type strain of Actinoplanes hulinensis is NEAU-M9(T) (= CGMCC 4.7036(T) = DSM 45728(T)).
    Antonie van Leeuwenhoek 11/2012; · 2.07 Impact Factor
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    ABSTRACT: A novel endophytic actinomycete, designated strain NEAU-J5(T) was isolated from snap bean root (Phaseolus vulgaris L.). Comparative analysis of the 16S rRNA sequence indicated that NEAU-J5(T) was phylogenetically related to members of the family Micromonosporaceae. The whole-cell sugars were galactose, mannose and glucose. The predominant menaquinones were MK-9(H4), MK-9(H6) and the major fatty acids were C16:0, C18:0, C17:1 ω7c, iso-C15:0 and C17:0. The phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. The DNA G+C content was 72.2 mol%. On the basis of the morphological, chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J5(T) represents a novel species of a new genus within the family Micromonosporaceae, for which the name Xiangella phaseoli gen. nov., sp. nov. is proposed. The type strain of Xiangella phaseoli is strain NEAU-J5(T) (=CGMCC 4.7038(T)=DSM 45730(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2012; · 2.11 Impact Factor