Wei Liang

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (45)151.19 Total impact

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    ABSTRACT: Today cancer is one of the most life-threatening diseases in the world. The conventional cancer therapies, including surgery, chemo- and radiation therapies, have some disadvantages, such as limited efficiency and significant side effects. It is necessary to develop new therapeutic treatments. Herein, we integrated the targeted photocatalytic and chemotherapy in a multifunctional drug-delivery platform. The aptamer-functionalized ZnO nanoparticles (NPs) were successfully synthesized. The anti-cancer drug was loaded in the aptamer-ZnO NP system. In vitro cell cytotoxicity experiments showed that combined therapy had a higher rate of death of cancer cells compared to that of single photocatalytic or chemotherapy. Furthermore, aptamer-functionalization could greatly increase the accumulation of nanoparticles within cancer cells and lead to better therapeutic effects. The results suggest that aptamer-functionalized semiconductor nanoparticles may have potential in the development of targeted photocatalytic and chemotherapy against cancer.
    Physical Chemistry Chemical Physics 07/2015; DOI:10.1039/c5cp02139a · 4.20 Impact Factor
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    ABSTRACT: The resistance of ovarian cancer to platinum-based chemotherapy is a critical issue in the clinical setting. The present study aimed to establish animal models to replicate this clinical condition, as well as to investigate the resistance mechanisms of ovarian cancer. A cisplatin (DDP)-resistant human ovarian cancer cell line, SKOV3/DDP, was screened, validated and injected subcutaneously into the neck of female nude mice. Following tumor establishment, the tumor was collected and cut into small sections, which were subsequently implanted into the ovaries of other nude mice. The growth of the orthotopic tumors was observed and the tumor-bearing mice were sacrificed and dissected. The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed. In the present study, 16 nude mice underwent orthotopic transplantation surgery and a tumor model was successfully established in 14/16 of the mice, with an in situ tumor formation rate of 87.5%. Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues. Therefore, the present study successfully established an orthotopic tumor transplantation model in nude mice using a c-Kit-positive DDP-resistant human ovarian cancer cell line. This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.
    Oncology letters 12/2014; 8(6):2611-2615. DOI:10.3892/ol.2014.2537 · 0.99 Impact Factor
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    ABSTRACT: Flavonoids are well known as a large class of polyphenolic compounds, which have a variety of physiological activities, including anti-influenza virus activity. The influenza A/WSN/33 infected A549 cells have been used to screen anti-influenza virus drugs from natural flavonoid compounds library. Unexpectedly, some flavonoid compounds significantly inhibited virus replication, while the others dramatically promoted virus replication. In this study, we attempted to understand these differences between flavonoid compounds in their antivirus mechanisms. Hesperidin and kaempferol were chosen as representatives of both sides, each of which exhibited the opposite effects on influenza virus replication. Our investigation revealed that the opposite effects produced by hesperidin and kaempferol on influenza virus were due to inducing the opposite cell-autonomous immune responses by selectively modulating MAP kinase pathways: hesperidin up-regulated P38 and JNK expression and activation, thus resulting in the enhanced cell-autonomous immunity; while kaempferol dramatically down-regulated p38 and JNK expression and activation, thereby suppressing cell-autonomous immunity. In addition, hesperidin restricted RNPs export from nucleus by down-regulating ERK activation, but kaempferol promoted RNPs export by up-regulating ERK activation. Our findings demonstrate that a new generation of anti-influenza virus drugs could be developed based on selective modulation of MAP kinase pathways to stimulate cell-autonomous immunity.
    Scientific Reports 11/2014; 4:7237. DOI:10.1038/srep07237 · 5.58 Impact Factor
  • Yinjian Zhou · Chunling Zhang · Wei Liang
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    ABSTRACT: RNA interference (RNAi) was intensively studied in the past decades due to its potential in therapy of diseases. The target specificity and universal treatment spectrum endowed siRNA advantages over traditional small molecules and protein drugs. However, the barriers exist in the blood circulation system and the disease tissues blocked the actualization of RNAi effect, which raised function versatility requirements to siRNA therapeutic agents. Appropriate functionalization of siRNAs are necessary to break through these barriers and target to disease tissues in local or systemic targeted application. In this review, we summarized barriers exist in the delivery process and popular functionalized technologies for siRNA such as chemical modification and physical encapsulation. Preclinical targeted siRNA delivery and the current status of siRNAs based RNAi therapeutic agents in clinical trial were reviewed and finally the future of siRNA delivery was proposed. The valuable experience from the siRNA agents delivery study and the RNAi therapeutic agents in clinical trial paved ways for practical RNAi therapeutics to emerge early.
    Journal of Controlled Release 05/2014; 193. DOI:10.1016/j.jconrel.2014.04.044 · 7.26 Impact Factor
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    ABSTRACT: Proteolytic enzymes in the gut represent one of the biggest barriers against oral delivery of therapeutic proteins and peptides. In the current study, we explored the effect of polyethylene glycol 400 (PEG 400), a commonly used crowing agent, on insulin degradation mediated by α-chymotrypsin (α-CT). Without PEG 400, insulin was quickly cleaved by α-CT to generate inactive degradation products. In comparison, incorporation of PEG 400 resulted in reaction mixtures with retained biological activity. The analysis on the conformation of α-CT and the local environment of the enzyme's active site unraveled that PEG 400 altered the conformation of α-CT to prevent the inactivation of insulin via stabilization of active intermediates. These findings indicated that PEG 400 may provide a promising addition toward oral delivery of insulin.
    Molecular Pharmaceutics 04/2014; 11(10). DOI:10.1021/mp500001n · 4.79 Impact Factor
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    ABSTRACT: This study used different methods to establish an animal model of orthotopic transplantation for ovarian cancer to provide an accurate simulation of the mechanism by which tumor occurs and develops in the human body. We implanted 4T1 breast cancer cells stably-transfected with luciferase into BALB/c mice by using three types of orthotopic transplantation methodologies: (1) cultured cells were directly injected into the mouse ovary; (2) cell suspension was initially implanted under the skin of the mouse neck; after tumor mass formed, the tumor was removed and ground into cell suspension, which was then injected into the mouse ovary; and (3) a subcutaneous tumor mass was first generated, removed, and cut into small pieces, which were directly implanted into the mouse ovary. After these models were established, in vivo luminescence imaging was performed. Results and data were compared among groups. Orthotopic transplantation model established with subcutaneous tumor piece implantation showed a better simulation of tumor development and invasion in mice. This model also displayed negligible response to artificial factors. This study successfully established an orthotopic transplantation model of ovarian cancer with high rates of tumor formation and metastasis by using subcutaneous tumor pieces. This study also provided a methodological basis for future establishment of an animal model of ovarian cancer in humans.
    01/2014; 8(1). DOI:10.1007/s11684-014-0315-5
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    ABSTRACT: Since polymeric micelles are promising and have potential in drug delivery systems, people have become more interested in studying the compatibility of polymeric carriers and drugs, which might help them to simplify the preparation method and increase the micellar stability. In this article, we report that cationic amphiphilic drugs can be easily encapsulated into PEGylated phospholipid (PEG-PE) micelles by self-assembly method and that they show high encapsulation efficiency, controllable drug release and better micellar stability than empty micelles. The representative drugs are doxorubicin and vinorelbine. However, gemcitabine and topotecan are not suitable for PEG-PE micelles due to lack of positive charge or hydrophobicity. Using a series of experiments and molecular modelling, we figured out the assembly mechanism, structure and stability of drug-loaded micelles, and the location of drugs in micelles. Integrating the above information, we explain the effect of the predominant force between drugs and polymers on the assembly mechanism and drug release behaviour. Furthermore, we discuss the importance of pKa and to evaluate the compatibility of drugs with PEG-PE in self-assembly preparation method. In summary, this work provides a scientific understanding for the reasonable designing of PEG-PE micelle-based drug encapsulation and might enlighten the future study on drug-polymer compatibility for other polymeric micelles.
    Philosophical Transactions of The Royal Society A Mathematical Physical and Engineering Sciences 10/2013; 371(2000):20120309. DOI:10.1098/rsta.2012.0309 · 2.86 Impact Factor
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    ABSTRACT: Polymeric micelles have been proven to be a promising nano-sized system for drug delivery. Understanding its in vivo behaviors at the whole body, tissue and cellular levels is critical for translating this drug delivery system into clinical practice. In this study, the 14.5nm micelles made of polyethylene glycol-phosphatidylethanolamine (PEG-PE) for delivery of doxorubicin and vinorelbine were investigated. Using confocal and two-photon microscopy imaging of live mice or tissue sections, we observed that after systemically administration, the fluorescently labeled PEG-PE micelles encapsulating doxorubicin migrated through blood vessels in entirety into the interstitial tissue, collected by lymphatic vessels, and accumulated in lymph nodes. Importantly, encapsulated drugs such as vinorelbine (Nanovin), preferentially accumulate in lymph nodes when compared to the free drugs. Moreover, the in vivo bioluminescent imaging showed that Nanovin significantly reduced lymph node metastasis rate (P<0.05) in 4T1-luc2 murine breast tumor bearing mice. Finally, we observed that Nanovin enhanced antitumor activity against primary tumors and lung metastases while having low toxicity in various 4T1 tumor models. This study suggests that PEG-PE micelle is a promising drug delivery system for the treatment of lymphatic metastases, and may also have important applications in other lymphatic system-related diseases.
    Journal of Controlled Release 07/2013; 171(2). DOI:10.1016/j.jconrel.2013.07.005 · 7.26 Impact Factor
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    ABSTRACT: The differentiation of periodontal ligament (PDL) progenitor cells is important for maintaining the homeostasis of PDL tissue and alveolar bone. Vitamin C (VC), a water-soluble nutrient that cannot be biosynthesized by humans, is vital for mesenchymal stem cells differentiation and plays an important role in bone remodeling. Therefore, the objective of this study was to determine the function and mechanism of VC in PDL progenitor cells osteogenic differentiation at the molecular level. We demonstrated that VC could induce the osteogenic differentiation and maturation of PDL progenitor cell without other osteogenic agents. During the process, VC preferentially activated ERK1/2 but did not affect JNK or p38. Co-treatment with ERK inhibitor effectively decreased the Vitamin C-induced expression of Runx2. ERK inhibitor also abrogated Vitamin C-induced the minimized nodules formation. PELP1, a nuclear receptor co-regulator, was up-regulated under VC treatment. PELP1 knockdown inhibited ERK phosphorylation. The overexpression of PELP1 had a positive relationship with Runx2 expression. Taken together, we could make a conclude that VC induces the osteogenic differentiation of PDL progenitor cells via PELP1-ERK axis. Our finding implies that VC may have a potential in the regeneration medicine and application to periodontitis treatment.
    Protein & Cell 07/2013; 4(8). DOI:10.1007/s13238-013-3030-0 · 2.85 Impact Factor
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    ABSTRACT: The effect of the anti-inflammatory flavonoid chrysin on osteogenesis was determined in preosteoblast MC3T3-E1 cells. Results demonstrated that chrysin could induce osteogenic differentiation in the absence of other osteogenic agents. Chrysin treatment promoted the expression of transcription factors (Runx2 and Osx) and bone formation marker genes (Col1A1, OCN, and OPN) as well as enhanced the formation of mineralized nodules. During osteogenic differentiation, chrysin preferentially activated ERK1/2, but not JNK nor the p38 MAPKs. Further experiments with inhibitors revealed the co-treatment of U0126, PD98059, or ICI182780 (a general ER antagonist) with chrysin effectively abrogated the chrysin-induced osteogenesis and ERK1/2 activation. Thus, the effect of chrysin on osteogenesis is ERK1/2-dependent and involves ER. Therefore, chrysin has the significant potential to enhance osteogenesis for osteoporosis prevention and treatment.
    Protein & Cell 06/2013; 4(7). DOI:10.1007/s13238-013-3003-3 · 2.85 Impact Factor
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    ABSTRACT: Prostate specific membrane antigen (PSMA) is overexpressed on prostate tumor cells and the neovascular endothelia various solid tumors. A bivalent immunotoxin generated by fusing a fold-back single-chain diabody derived from the Fv fragments of an anti-PSMA monoclonal antibody with a truncated diphtheria toxin (DT) containing the activity and translocation domains [A-dmDT390-scfbDb(PSMA)] might be suitable for targeted therapy of tumors that overexpress PSMA. In this study, a PSMA-positive and a PSMA-negative prostate cancer cell lines were treated with immunotoxin A-dmDT390-scfbDb(PSMA) in order to study the tumor targeting specificity and therapeutic potential of the immunotoxin. The cellular uptake and selective toxicity of the immunotoxin were evident in monolayer cultures of PSMA-positive LNCaP prostate cancer cells but not in cultures of PSMA-negative PC-3 prostate cancer cells. Cellular accumulation of A-dmDT390-scfbDb(PSMA) increased with increasing incubation times and concentrations in LNCaP cells. The proportion of apoptotic LNCaP cells increased upon incubation with increasing doses of the fold-back immunotoxin. Optical imaging and MRI with the Alexa Fluor 680-labeled A-dmDT390-scfbDb(PSMA) confirmed the specific targeting and therapeutic efficacy of this immunotoxin towards PSMA-positive LNCaP solid tumor xenografts in athymic nude mice.
    05/2013; 2(5). DOI:10.1002/adhm.201200254
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    ABSTRACT: It is common that many enterprises tend to operate several datacenters applying virtualization technologies from different vendors. However, for virtual machine migration across different vendors, currently there is no feasible method to transfer the corresponding network policies as well. In this paper, we show a cross-vendor network policy adaptor (CVNPA), which is able to cooperate with today's cloud computing platforms and to provide seamless network policy migration across virtual switches from different vendors. We experiment CVNPA in our simulated cloud computing environment with OpenStack as their cloud computing platform. The results show that CVNPA can transfer corresponding network policy over heterogeneous virtual switches efficiently and automatically during the process of VM migration with little overhead.
    Distributed Computing Systems Workshops (ICDCSW), 2013 IEEE 33rd International Conference on; 01/2013
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    ABSTRACT: Highly virtualized data center have placed many new and unique requirements on the networking fabric. Conventional network protocols limit the scale, latency, throughput of cloud networks. To solve these problems, the large layer 2 technology is proposed. The large layer 2 technology such as TRILL and SPB has obvious advantages in scalability, convergence and resource utilization compared with the traditional layer 2 technology. However, the disadvantages of employing the large layer 2 technology are barely addressed. To study the overhead of large layer 2 networking, we setup a data center environment with 6 core switches and conduct experiments to compare the performance of the representative large layer 2 protocol-TRILL and the typical layer 2 technology-STP. We also evaluate the intra-VLAN forwarding and inter-VLAN forwarding of TRILL. From our experiments, it is observed that the forwarding efficiency of TRILL is 30.16% lower than STP on average. It is also observed that the inter-VLAN traffic forwarding of TRILL averagely costs about 54% more time and consumes about 5% more CPU than intra-VLAN with a fixed packet generation rate. This is the first study towards unveiling the inefficiency of large layer 2 approach in data center networks as far as we know.
    Innovative Computing Technology (INTECH), 2013 Third International Conference on; 01/2013
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    ABSTRACT: Epithelial to mesenchymal transition (EMT) promotes cellular motility, invasiveness and metastasis during embryonic development and tumorigenesis. Transforming growth factor-β (TGF-β) signaling pathway is a key regulator of EMT. A lot of evidences suggest that this process is Smad3-dependent. Herein we showed that exposure of aspc-1 and panc-1 pancreatic cancer cells to TGF-β1 resulted in characteristic morphological alterations of EMT, and enhancement of cell motility and gemcitabine (Gem) resistance along with an up-regulation of EMT markers genes such as vimentin, N-cadherin, MMP2 and MMP9. Naringenin (Nar) down-regulated EMT markers expression in both mRNA and protein levels by inhibiting TGF-β1/Smad3 signal pathway in the pancreatic cancer cells. Consequently, Nar suppressed the cells migration and invasion and reversed their resistance to Gem.
    PLoS ONE 12/2012; 7(12):e50956. DOI:10.1371/journal.pone.0050956 · 3.23 Impact Factor
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    ABSTRACT: The fragment of viral protein R (Vpr), Vpr13-33, plays an important role in regulating nuclear importing of HIV through ion channel formation with a leucine-zipper-like α-helical conformation. Herein we report an approach to reduce cytotoxicity of Vpr13-33 by graphene oxide induced conformation change and aggregation. Preferential adsorption of Vpr13-33 on graphene oxide accompanied by conformation change from α-helix to β-sheet structures has been observed by using atomic force microscopy (AFM) and circular dichroism (CD). The submolecular structures of the Vpr13-33 peptide assembly on graphite surface have been identified by using scanning tunneling microscopy (STM), which confirms the β-sheet structures of Vpr13-33 on graphene oxide surface. The reduced cytotoxicity of Vpr13-33 to neuroblastoma cells and T cells are detected by MTT assay, which could be associated with the conformation change and stimulated aggregation of Vpr13-33 upon addition of graphene oxide through hydrophobic interaction. Furthermore, fluorescent leakage assay by using large unilamellar vesicles (LUVs) indicated that the GO reduced Vpr13-33-induced cytotoxicity could be associated with the inhibited "pore forming" function of Vpr13-33 by conformation change and aggregation.
    Biomaterials 11/2012; 34(4). DOI:10.1016/j.biomaterials.2012.10.067 · 8.31 Impact Factor
  • Ruixing Li · Huirong Fan · Yuan Gu · Duanyun Si · Wei Liang
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    ABSTRACT: A simple, robust, and sensitive high-performance liquid chromatography tandem mass spectrometry method was developed and validated for determination of a new nonpeptidic glucose-like peptide-1 receptor (GLP-1R) agonist 1,3-bis(4-((p-butoxycarbonyl)amino)benzamido)-2,4-bis(m-methoxy-p-(thiophene-2-methanoyl)phenyl)-cyclobutane-1,3-dicarboxylic acid (Boc5) in rat plasma using wogonin as internal standard (IS). After a simple protein precipitation with methanol, chromatographic separation was achieved on an Atlantis T3 (100 mm × 4.6 mm, 3.5 μm) analytical column eluted by mobile phase consisting of methanol, acetonitrile, and 20 mM ammonium formate addition to 2% formic acid (39:39:22, v/v/v) at flow rate of 0.6 mL/min for 7 min. The quantitation analysis was performed using multiple reaction monitoring at the transitions of m/z 1,077.2 → 784.4 for Boc5 and m/z 285.1 → 270.4 for IS in positive ion mode with electrospray ionization source on an AB SCIEX API 4000 QTRAP mass spectrometer. Good linearity was achieved over the concentration range of 2–2,000 ng/mL with lower limit of quantification at 2 ng/mL. Intra- and interday precisions were less than 7.3 %, and accuracy ranged from −3.6 to 3.0 %. The mean recovery of Boc5 was 93.4–100.2 %, and the matrix effect of Boc5 was 88.0–93.8 %. The validated method was then successfully applied to a pharmacokinetic study of Boc5 in rats.
    Chromatographia 07/2012; 75(13-14). DOI:10.1007/s10337-012-2248-y · 1.37 Impact Factor
  • Jing Wang · Xiaocui Fang · Wei Liang
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    ABSTRACT: The rapid developments in nanotechnology have brought with them a deep concern over the safety of nanomaterials. Investigating the molecular mechanisms underlying their toxicity in different cell lines will help us better understand and apply nanomaterials appropriately. Poly(ethylene glycol)-phosphoethanolamine (PEG-PE) is an FDA-approved nonionic diblock copolymer and is widely used in drug delivery systems. Here, we find that PEG-PE accumulates in the endoplasmic reticulum (ER) and induces ER stress and that cancer cells and normal cells have different cell fates as a result of this stress. In A549 cancer cells, PEG-PE damages ER functions and triggers apoptosis by activating proapoptotic UPR signaling and high expression of cell death effector CHOP and proapoptotic Bax/Bak. In addition, PEG-PE-induced ER stress also up-regulates lipid synthesis and triggers lipid droplet formation in cancer cells. By contrast, in MRC-5 and 293T cells, high expression of the UPR feedback protein GADD34 which inhibits proapoptotic UPR signaling, and antiapoptotic Bcl-2 and Bcl-xl which down-regulate Bax/Bak, protect these normal cells from PEG-PE-induced apoptosis. When gadd34, bcl-2, or bcl-xl is knocked down, apoptosis occurs in PEG-PE-treated normal cells. In summary, we demonstrate the safety of PEG-PE in normal cells and elaborate the molecular mechanism underlying its nanotoxicity in cancer cells. This study implies PEG-PE-based drug delivery system has the potential to alter the sensitivity of cancer cells to some chemotherapeutic agents by selectively activating unfolded protein response (UPR) in cancer cells, and it also provides a useful foundation for research on ER stress-induced nanotoxicity and other lipid-based nanomaterials.
    ACS Nano 05/2012; 6(6):5018-30. DOI:10.1021/nn300571c · 12.88 Impact Factor
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    ABSTRACT: We report a study of nanoribbons of quercetin, a phase I clinical trial anticancer drug, and their inhibitory effects on cancer cell proliferation. Novel quercetin nanoribbons have been prepared by atmospheric pressure physical vapor deposition (PVD). The nanostructures have been characterized by optical microscopy, scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy, etc. Significantly enhanced solubility in PBS solution and increased drug release rate have been observed for quercetin nanoribbons in comparison to those of quercetin powder. The observed increase of inhibitory effects of quercetin nanoribbons on 4T1 cancel cell growth is correlated with an improvement in their solubility and drug release behavior.
    Nanoscale 03/2012; 4(6):2078-82. DOI:10.1039/c2nr12013e · 7.39 Impact Factor
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    ABSTRACT: To investigate the influences of stability of doxorubicin (DOX) retained in PEG-PE/HSPC micelles on its biodistribution, toxicity and anti-tumor activity in mice. We incorporated HSPC into PEG-PE micelles at various molar ratios by a self-assembly procedure. Micelles were characterized by dynamic light scattering, transmission electron microscope, atomic force microscopy. Agarose gel electrophoresis assay was used to detect stable retention of DOX in micellar preparations. Biodistribution, toxicity and anti-tumor activity of doxorubicin encapsulated in PEG-PE/HSPC micelles in mice were investigated. HSPC incorporation not only changed the size and shape of PEG-PE micelles, but also decreased the ability of DOX stable retained in PEG-PE micelles, resulting in a great discrepancy in biodistribution, toxicity and anti-tumor activity among micellar DOX preparations. DOX encapsulated in PEG-PE micelles (M₁-DOX), with narrower size distribution and greater stability, demonstrated better cytotoxicity in vitro and low systemic toxicity with superior anti-tumor metastasis activity in vivo. Encapsulation of DOX into PEG-PE micelles showed the best therapeutic activity and lowest systemic toxicity compared to other HSPC-incorporated PEG-PE micellar preparations. Stable retention of drugs within micelles is important and is determined by compatibility between drugs and polymer blocks.
    Pharmaceutical Research 03/2012; 29(7):1977-89. DOI:10.1007/s11095-012-0725-5 · 3.95 Impact Factor
  • Jing Wang · Yiguang Wang · Wei Liang
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    ABSTRACT: Developing polymer micelles as drug delivery systems requires an in-depth understanding of how they interact with cells and deliver drugs across the cell membrane; however, this investigation faces many problems. In this study, we attempt to show how polyethylene glycol-phosphatidylethanolamine (PEG-PE) micelles deliver drugs across cell membranes by using a series of fluorescence techniques, including de-quenching, FRET and multi-labeling. We demonstrate that PEG-PE copolymers transfer to the cell membrane from micelles due to their amphiphilic properties, and are then internalized through nonspecific endocytosis and distributed in the endoplasmic reticulum and Golgi apparatus without affecting cellular ATP and viability. Hydrophilic drugs and/or hydrophobic dyes can be encapsulated in PEG-PE micelles, which are maintained in an intact form in culture medium before and after incubation with cells. These micelles disassemble and release their payloads at the cell membrane; released agents are accelerated to enter cells due to the increased membrane fluidity caused by PEG-PE insertion. Encapsulation of drugs in micelles does not change their intracellular distribution but increases their cellular accumulation. This investigation provides new insights into the mechanism of polymer micelle delivering drugs into cells, which are helpful for designing a true site-specific delivery carrier.
    Journal of Controlled Release 03/2012; 160(3):637-51. DOI:10.1016/j.jconrel.2012.02.021 · 7.26 Impact Factor

Publication Stats

618 Citations
151.19 Total Impact Points

Institutions

  • 2006–2014
    • Chinese Academy of Sciences
      • • Institute of Biophysics
      • • Institute of Computing Technology
      • • National Laboratory of Biomacromolecules
      Peping, Beijing, China
  • 2011
    • Beijing Institute Of Technology
      Peping, Beijing, China
  • 2008–2010
    • Technical Institute of Physics and Chemistry
      • Key Laboratory of Photochemical Conversion and Optoelectronic Materials
      Peping, Beijing, China