[Show abstract][Hide abstract] ABSTRACT: This study examined the effects of a 2-mo antioxidant vitamin treatment on acute hematological and hemorheological alterations induced by exhausting exercise; both sedentary and trained individuals were employed. Eighteen young male, human subjects (9 sedentary, 9 trained by regular exercise) participated in the study and performed an initial maximal aerobic cycle ergometer exercise with frequent blood sampling over a 24-h period and analysis of hematological and hemorheological parameters. All subjects were treated with an antioxidant vitamin A, C, and E regimen, supplemented orally for 2 mo, and then subjected to a second exercise test and blood sampling at the end of this period. In the sedentary group during the first testing period (before vitamin treatment), white blood cell counts and granulocyte percentages were increased at 2 h after the exercise test and remained elevated for 4-12 h. Red blood cell (RBC) deformability and aggregation were also altered by exercise in the sedentary group before vitamin treatment. However, none of these parameters in the sedentary group were altered by exercise after the 2-mo period of antioxidant vitamin treatment. With the exception of a transient rise in granulocyte percentage, these parameters were also not affected in the trained subjects before the vitamin treatment. Significant increases of RBC lipid peroxidation observed 12 h after the exercise test in both sedentary and trained subjects were also totally prevented by vitamin treatment. Our results indicate that antioxidant vitamin treatment is effective in preventing the inflammation-like response and coincident adverse hemorheological changes after an episode of exhausting exercise, and suggest that such changes may be related to exercise-induced death events.
[Show abstract][Hide abstract] ABSTRACT: The purpose of this study was to investigate the effect of mild chronic exercise on visual evoked potentials (VEPs). Twenty male Wistar rats were randomly divided into two groups: Control (C) and Exercise (E) groups. Exercise was performed on a motor-driven treadmill for 8 weeks. After 5 min of exercise, plasma lactic acid levels were determined. At the end of the experimental period, VEPs were recorded from E group twice: Five min (E-5 min) and 24 h (E-24 h) after the last bout of exercise. During visual evoked potential (VEP) recordings body temperature of the animals was kept constant to eliminate the effect of temperature changes. No difference was found between the lactic acid levels of two groups. The mean latencies of VEPs from E-5 min were shortened compared with the control group. The mean latencies of VEP components in E-24 h were observed to have returned to the control levels. Peak to peak amplitudes of VEPs were found to be unaltered among all measurements. We concluded that immediately after exercise, VEPs latencies were shortened independently from body temperature via unknown mechanisms. The latencies of VEPs were returned to control values after 24 h.
International Journal of Sports Medicine 08/2003; 24(5):359-62. DOI:10.1055/s-2003-40699 · 2.07 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Red blood cell (RBC) mechanical properties were investigated after swimming exercise in trained and untrained rats. A group of rats was trained for 6 wk (60 min swimming, daily), and another group was kept sedentary. Blood samples were obtained either within 5 min or 24 h after 60 min swimming in both groups. In the untrained rats, the RBC aggregation index decreased to 2.60 +/- 0.4 immediately after exercise from a control value of 6.73 +/- 0.18 (P < 0.01), whereas it increased to 13.13 +/- 0.66 after 24 h (P < 0.01). RBC transit time through 5-microm pores increased to 3.53 +/- 0.16 ms within 5 min after the exercise from a control value of 2.19 +/- 0. 07 ms (P < 0.005). A very significant enhancement (166%) in RBC lipid peroxidation was detected only after 24 h. In the trained group, the alterations in all these parameters were attenuated; there was a slight, transient impairment in RBC deformability (transit time = 2.64 +/- 0.13 ms), and lipid peroxidation was found to be unchanged. These findings suggest that training can significantly limit the hemorheological alterations related to a given bout of exercise. Whether this effect is secondary to the training-induced reduction in the degree of metabolic and/or hormonal perturbation remains to be determined.
Journal of Applied Physiology 07/2000; 88(6):2074-80. · 3.06 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Nitric oxide (NO) plays a major role in vascular regulation. Modulation of NO synthesis is known to influence blood pressure. Inhibition of NO synthesis by NG-nitro-L-arginine methyl ester (L-NAME; 72 mg/kg/day, p.o., 21 days) resulted in 60% increase in blood pressure in rats. Red blood cell (RBC) transit time measured by the cell transit analyzer increased significantly in the L-NAME treated group, in comparison to normotensive rats. RBC aggregation measured in autologous plasma, by a photometric rheoscope also increased significantly in the hypertensive rats. RBC cytosolic free calcium concentration was also significantly higher in the hypertensive animals. Incubation of RBC from hypertensive and control animals with NO donor, sodium nitroprusside (SNP; 10-1000 microM) for 60 minutes resulted in a dose-dependent decrease in RBC aggregation, however aggregation index was significantly higher in hypertensive group at each SNP concentration. Incubation with SNP had no effect on RBC deformability in the control group, while a slight decrease in RBC transit time was observed only at 10 microM SNP in the hypertensive group. These results imply that NO may play a role in the regulation of rheological properties of RBC and the alterations in these properties may at least in part be involved in the development of L-NAME induced hypertension.
Clinical hemorheology and microcirculation 02/2000; 22(4):267-75. · 2.24 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Pregnant swiss albino rats were divided to three groups as control (C), cadmium (Cd) and non-cadmium (NCd) groups. Control animals were received tap water while the rats of Cd group were received Cd as CdC12 in their drinking water during the experimental period. On the other hand, the NCd group was given Cd during pregnancy, but given tap water after birth. Twenty-two days after birth, fourteen rats (for each group) were taken from their mothers and continued to be treated with Cd (Cd group) or tap water (C and NCd groups) for an additional 38 days. After the experimental period, flash VEPs and EEGs of three groups were recorded and amplitude spectral analysis was computed by Transient Response-Frequency characteristics (TRFC) method. The mean amplitude (dB) of 1-3.5 and 14-20 Hz frequency bands for right response whereas 1-3.5, 4-7, 8-13 and 14-20 Hz frequency bands for left response of VEPs were decreased in Cd group compared with control group. On the other hand, significant differences were observed between Cd and control groups in all the frequency bands of EEGs except 6-8 Hz.
International Journal of Neuroscience 05/1996; 85(3-4):173-84. DOI:10.3109/00207459608986681 · 1.52 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Pregnant Swiss albino rats were divided into three groups: control (C), cadmium (Cd) and non-cadmium (NCd) groups. Control animals received tap water; the Cd rats received Cd as CdCl2 in their drinking water during the experimental period, while the NCd group was given Cd during pregnancy, and given tap water after birth. Twenty-two days after birth, 15 rats (for each group) were taken from their mothers and continued to be treated with Cd (Cd group) or tap water (C and NCd groups) for an additional 38 days. After the treatment period, somatosensory evoked potentials (SEPs) of the three groups were recorded from central (Cz) referenced to frontal (Fz) following left posterior tibial nerve (PTN) stimulation. Amplitude spectra of SEPs were computed by fast Fourier transform (FFT) algorithm. There was a significant amplitude decrease in 1-3.5 Hz in the NCd group and 1-3.5 and 14-20 Hz frequency bands of the Cd group compared with the control group.
Journal of basic and clinical physiology and pharmacology 02/1996; 7(2):137-49. DOI:10.1515/JBCPP.1922.214.171.124