ABSTRACT: To understand the molecular and epidemic characteristics of Hantavirus S85-46 isolated from Sichuan province.
RT-PCR products were amplified from S85-46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
The homology of M segment of S85-46 strain and HTN type strain varied from 84.1%-99.7%, compared to 70.4%-70.9% with SEO strains. This result indicated that S85-46 strain belonged to hantavirus. The nucleotide sequence analyses indicated that S85-46 strain, LR1 strain and 76-118 strain were highly homologous. The two latter strains were isolated from Shaanxi province of China and South Korea respectively, which were far from Sichuan province of China. The nucleotide sequence analyses of S segment was similar to M segment.
The epidemic strains isolated from different regions may have been highly homologous.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 01/2002; 22(6):449-51.
ABSTRACT: Study on the complete genome sequence of Hantavirus Z10 strain which has been applied for inactivated vaccine production in China, to assess its molecular characteristics and the diversity with other hantaviruses.
The total RNA were prepared from Z10 virus infected cells and the RT-PCR products was cloned into T vector, sequenced and analyzed by using DNASTAR software.
The Z10 complete genome, L segment is 6,553, M segment is 3,615, S segment is 1,701 nucleotides in length, with a single open reading frame encoding 2,151, 1,135, 429 amino acids respectively. Sequence homology comparison showed that the 3 segment nucleotide of Z10 strain were close to HTN type virus, but only 83.6-87.4% homology with other HTN viruses at the nucleotide level. The phylogenetic analysis was made on their nucleotide and amino acid sequences.
The results firstly demonstrates that Z10 strain is a new subtype of the Hantaan(HTN) type.
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 07/2001; 15(2):112-5.
ABSTRACT: To develop a kind of new Japanese Encephalitis (JE) vaccine prepared on Vero cells.
A JE attenuated virus strain SA14-14-2 was adapted on Vero cells for preparation of purified inactivated vaccine. Comparison of the growth curves of SA14-14-2 in roller bottle and in spinner flask was made. After the virus inoculation and absorption on Vero cells for 2 hours, cultures were replaced with serum-free MEM, the culture supernatants were harvested on day 2,4,6 after inoculation. The virus fluids were pooled, concentrated by 8% PEG, and purified on 15%-60% sucrose density gradients. The purified virus was inactivated with 0.02% formalin.
It showed that virus titer was higher and maintained longer in roller bottle. Mice vaccinated twicely with 0.5 microgram dose of the purified inactivated vaccine induced neutralizing antibody titers equal to that of the mice vaccinated with primary hamster kidney inactivated vaccine.
This Vero cell prepared purified and inactivated JE vaccine made by SA14-14-2 strain could be used for human as a kind of new JE vaccine.
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 07/1999; 13(2):191-3.