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Zhibin Hu,
Chen Wu,
Yongyong Shi,
Huan Guo,
Xueying Zhao,
Zhihua Yin,
Lei Yang,
Juncheng Dai,
Lingmin Hu,
Wen Tan, [......],
Zhengdong Zhang,
Feng Chen,
Xinru Wang,
Li Jin,
Jiachun Lu,
Baosen Zhou,
Daru Lu,
Tangchun Wu,
Dongxin Lin,
Hongbing Shen
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ABSTRACT: Lung cancer is the leading cause of cancer-related deaths worldwide. To identify genetic factors that modify the risk of lung cancer in individuals of Chinese ancestry, we performed a genome-wide association scan in 5,408 subjects (2,331 individuals with lung cancer (cases) and 3,077 controls) followed by a two-stage validation among 12,722 subjects (6,313 cases and 6,409 controls). The combined analyses identified six well-replicated SNPs with independent effects and significant lung cancer associations (P < 5.0 × 10(-8)) located in TP63 (rs4488809 at 3q28, P = 7.2 × 10(-26)), TERT-CLPTM1L (rs465498 and rs2736100 at 5p15.33, P = 1.2 × 10(-20) and P = 1.0 × 10(-27), respectively), MIPEP-TNFRSF19 (rs753955 at 13q12.12, P = 1.5 × 10(-12)) and MTMR3-HORMAD2-LIF (rs17728461 and rs36600 at 22q12.2, P = 1.1 × 10(-11) and P = 6.2 × 10(-13), respectively). Two of these loci (13q12.12 and 22q12.2) were newly identified in the Chinese population. These results suggest that genetic variants in 3q28, 5p15.33, 13q12.12 and 22q12.2 may contribute to the susceptibility of lung cancer in Han Chinese.
Nature Genetics 07/2011; 43(8):792-6. · 35.53 Impact Factor
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ABSTRACT: Mesophase pitch-based carbon foams were prepared with supercritical toluene. The effects of the foaming conditions, such as the solvent proportions, temperatures, initial pressures, and pressure drop rates, on the cell densities and mean cell diameters of the carbon foams were studied. The foaming mechanism was analyzed with the theories of homogeneous nucleation. The carbon foams varied in cell densities and cell structure from three-dimensional interconnected to closed sizing in the range 200 to 800 μm when they were generated under different conditions. The carbon foam structure could be optimized with cell densities of 10–16 × 103 cm−3 and cell diameters from 400 to 450 μm, under the conditions where the temperature was 588 K, the initial pressure was 3.0 MPa, the pressure drop rate was 1.5 MPa/s, and the solvent proportion was 30 wt %. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Journal of Applied Polymer Science 02/2011; 121(1):336 - 341. · 1.29 Impact Factor
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ABSTRACT: The adsorption properties of Cu(2+)-loaded montmorillonite clays (MMT-Cu) for Escherichia coli K88 as a function of time, bacteria concentrations, pH, ionic strength and temperature were investigated. The results showed that the bacteria adsorption onto MMT-Cu surface reached equilibrium after 90 min. The percentages of E. coli K88 adsorbed onto the surfaces of MMT-Cu and montmorillonite clays (MMT) at equilibrium were 88.9% and 56.5%, respectively. Scanning electron microscopy revealed that a lot of E. coli K88 adhered to the surface of MMT-Cu. The zeta potential of MMT-Cu was relatively high as compared to that of MMT. The adsorptive ability of MMT-Cu for E. coli K88 was higher than that of MMT (P < 0.05). Moreover, pH, ionic strength and temperature produced a strong influence on the extent of E. coli K88 adsorption to surface of MMT-Cu and MMT. The mechanism of adsorption of E. coli onto MMT-Cu may involve electrostatic attraction and physiochemical properties of bacterial cell walls and minerals surfaces.
Journal of Environmental Sciences 01/2011; 23(11):1808-15. · 1.66 Impact Factor
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Zi-Jiang Chen,
Han Zhao,
Lin He,
Yuhua Shi,
Yingying Qin,
Yongyong Shi, Zhiqiang Li,
Li You,
Junli Zhao,
Jiayin Liu, [......],
Cuifang Hao,
Chun-E Ren,
Yajie Zhang,
Shiling Chen,
Wei Zhang,
Aijun Yang,
Junhao Yan,
Yuan Li,
Jinlong Ma,
Yueran Zhao
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ABSTRACT: Polycystic ovary syndrome (PCOS) is a common metabolic disorder in women. To identify causative genes, we conducted a genome-wide association study (GWAS) of PCOS in Han Chinese. The discovery set included 744 PCOS cases and 895 controls; subsequent replications involved two independent cohorts (2,840 PCOS cases and 5,012 controls from northern Han Chinese; 498 cases and 780 controls from southern and central Han Chinese). We identified strong evidence of associations between PCOS and three loci: 2p16.3 (rs13405728; combined P-value by meta-analysis P(meta) = 7.55 × 10⁻²¹, odds ratio (OR) 0.71); 2p21 (rs13429458, P(meta) = 1.73 × 10⁻²³, OR 0.67); and 9q33.3 (rs2479106, P(meta) = 8.12 × 10⁻¹⁹, OR 1.34). These findings provide new insight into the pathogenesis of PCOS. Follow-up studies of the candidate genes in these regions are recommended.
Nature Genetics 01/2011; 43(1):55-9. · 35.53 Impact Factor
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ABSTRACT: Using matrices to express logical relations is one of the foundations of the approach proposed in this book. In this chapter
we express a statement or a logical variable as a vector, and express a logical operator via its structure matrix. The action
of a logical operator on logical variables can then be expressed as the semi-tensor product of the structure matrix of the
operator with vectors of logical arguments(Cheng, Proc. 4th International Congress of Chinese Mathematicians, pp.641–668,
Higher Edu. Press, Int. Press, Hangzhou, 2007; Cheng and Qi, Semi-tensor Product of Matrices—Theory and Applications, Science Press, Beijing, 2007).
11/2010: pages 55-65;
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ABSTRACT: In this chapter we give a brief introduction to random Boolean networks. This a topic of considerable current interest and
the results are abundant, so a detailed discussion is beyond the scope of this book. What we present here are just the basics.
11/2010: pages 431-450;
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ABSTRACT: In this chapter, the problem of solving infinitely repeated games by means of Nash or sub-Nash equilibria is considered. Using
the algebraic form of a logical dynamical system, a strategy with finite memory can be expressed as a logical matrix. The
Nash equilibria are then computable, providing Nash solutions to the given game. When the Nash equilibrium does not exist,
sub-Nash equilibria and ε-tolerance solutions may provide a reasonable solution to the game. Common Nash or sub-Nash equilibria make different memory-length
strategies comparable. An optimal or sub-optimal solution can then be obtained. Certain algorithms are proposed. This chapter
is based on Cheng et al. (Nash and sub-Nash solutions to infinitely repeated games, 2010; Proc. IEEE CDC’2010, 2010).
11/2010: pages 409-429;
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Tao Li, Zhiqiang Li,
Peng Chen,
Qian Zhao,
Ti Wang,
Ke Huang,
Junyan Li,
You Li,
Jie Liu,
Zhen Zeng,
Guoyin Feng,
Lin He,
Yongyong Shi
[show abstract]
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ABSTRACT: Schizophrenia is a complex major psychiatric disorder affecting ∼1% of the world population. Recently, in a genome-wide association study and a follow-up in Caucasians, Stefansson et al. examined 7662 schizophrenic cases and 29053 normal control subjects and reported seven common single nucleotide polymorphisms (SNPs) that were significantly (>10(-8)) associated with schizophrenia.
To investigate whether these risk SNPs were significantly associated in Han Chinese, we analyzed the seven SNPs in 2496 schizophrenia patients and 5184 normal control subjects. Because only three of the seven SNPs were polymorphic in Han Chinese, we genotyped two additional common SNPs from the same risk regions.
Three SNPs, rs6932590 (p = .00096), rs3131296 (p = 1.29 × 10(-6)), and rs3130375 (p = 1.76 × 10(-5)), mapping to the major histocompatibility complex region and one SNP rs2958182 (p = 3.64 × 10(-6)) located in the TCF4 gene were significant in our sample set. A meta-analysis using published genome-wide association study results also supported our findings.
Our results confirm that common risk factors in the major histocompatibility complex region and TCF4 gene are associated with schizophrenia in Han Chinese, but our results fail to show an association with SNP rs12807809 in the NRGN gene.
Biological psychiatry 10/2010; 68(7):671-3. · 8.93 Impact Factor
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Jie Liu,
Junyan Li,
Tao Li,
Ti Wang,
You Li,
Zhen Zeng, Zhiqiang Li,
Peng Chen,
Zhiwei Hu,
Lingqing Zheng,
Jue Ji,
He Lin,
Guoyin Feng,
Yongyong Shi
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ABSTRACT: Previous studies have reported that the cytotoxic T lymphocyte antigen-4 (CTLA-4) gene, which is related to immunological function such as T-cell regulation, is associated with psychiatric disorders. In this study, we studied the relationship between CTLA-4 and three major psychiatric disorders, schizophrenia, major depressive disorder and bipolar disorder in the Chinese Han population. We recruited 1140 schizophrenia patients, 1140 major depressive disorder patients, 1140 bipolar disorder patients, and 1140 normal controls to examine the risk conferred by 6 tag SNPs (rs231777, rs231775, rs231779, rs3087243, rs5742909, rs16840252) in the CTLA-4 gene. We found that rs231779 conferred a risk for schizophrenia (P(allele)=0.0003, P(genotype)=0.0016), major depressive disorder (P(allele)=0.0006, P(genotype)=0.0026) and bipolar disorder (P(allele)=0.0004, P(genotype)=0.0018). In addition, rs231777 and rs16840252 had a significant association with schizophrenia (rs231777: P(allele)=0.0201, rs16840252: P(allele)=0.0081, P(genotype)=0.0117), and rs231777 had significant association with bipolar disorder (rs231777: P(allele)=0.0199). However, after 10,000 permutations, only rs231779 remained significant (schizophrenia: P(allele)=0.0010, P(genotype)=0.0145, major depressive disorder: P(allele)=0.0010, P(genotype)=0.0201, bipolar disorder: P(allele)=0.0008, P(genotype)=0.0125). Our results suggest that shared common risk factors for schizophrenia, major depressive disorder and bipolar disorder exist in the CTLA-4 gene in the Chinese Han population.
Brain Behavior and Immunity 10/2010; 25(3):429-33. · 4.72 Impact Factor
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ABSTRACT: The objective of this study was to investigate the effects of different corn silage : alfalfa silage ratios and full fat extruded soybeans on milk composition, especially that of conjugated linoleic acids content in milk fat and performance of dairy cows. Sixty Holstein cows were divided into 6 groups for a 12 week study. Treatments were arranged in a 2 × 3 factorial with 0 or 5% full fat extruded soybeans meal (dry matter basis) and three forage treatments (dry matter basis): (1) 13.5% corn silage, (2) 10.1% corn silage and 4.1% alfalfa silage, (3) 6.8% corn silage and 7.8% alfalfa silage. Full fat extruded soybeans addition increased the ether extract in diets and content of conjugated linoleic acids in milk, but it had no effect on fat, protein and lactose content in milk. Milk fat and lactose were not affected by replacing corn silage with alfalfa silage in diets. Increasing alfalfa silage content in the diets increased milk yield and milk content of conjugated linoleic acids and protein. Results from this study suggested that more alfalfa silage can be used in the diet of cow as a good source of forages.
AFRICAN JOURNAL OF BIOTECHNOLOGY 09/2010; 9:5465-5464. · 0.57 Impact Factor
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Cell Research 07/2010; 20(7):854-7. · 8.19 Impact Factor
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Ruiqiang Li,
Wei Fan,
Geng Tian,
Hongmei Zhu,
Lin He,
Jing Cai,
Quanfei Huang,
Qingle Cai,
Bo Li,
Yinqi Bai, [......],
Jingxiang Li,
Lars Bolund,
Karsten Kristiansen,
Gane Ka-Shu Wong,
Maynard Olson,
Xiuqing Zhang,
Songgang Li,
Huanming Yang,
Jian Wang,
Jun Wang
Nature 02/2010; 463(7284):1106. · 36.28 Impact Factor
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Ruiqiang Li,
Wei Fan,
Geng Tian,
Hongmei Zhu,
Lin He,
Jing Cai,
Quanfei Huang,
Qingle Cai,
Bo Li,
Yinqi Bai, [......],
Jingxiang Li,
Lars Bolund,
Karsten Kristiansen,
Gane Ka-Shu Wong,
Maynard Olson,
Xiuqing Zhang,
Songgang Li,
Huanming Yang,
Jian Wang,
Jun Wang
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ABSTRACT: Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.
Nature 12/2009; 463(7279):311-317. · 36.28 Impact Factor
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Ronglin Che,
Wei Tang,
Liyan Shao,
Qian Zhao,
Ke Huang,
Ruqi Tang, Zhiqiang Li,
Xinzhi Zhao,
Weidong Ji,
Guoquan Zhou,
Guoyin Feng,
Lin He,
Yongyong Shi
Psychiatric genetics 08/2009; 19(5):279-80. · 2.33 Impact Factor
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Cell Research 05/2009; 19(4):519-23. · 8.19 Impact Factor
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ABSTRACT: The cyprinid fish genus Sinocyclocheilus, as the most cavefish rich genus, includes many species showing striking adaptation to caves and convergent reduction or
even loss of eyes and pigmentation. RH1 is responsible for dim vision. In order to explore the evolution of RH1 gene in this genus, we sequenced the complete gene from 28 individuals of 16 representative species of Sinocyclocheilus, with cave and surface species included. Phylogenetic analyses supported the monophyly of Sinocyclocheilus and polyphyly of the cave species. Codon models implemented in PAML were used to infer the evolution of RH1. We found that Sinocyclocheilus had a significantly higher evolutionary rate for amino acids than other cyprinid fishes compared, which might be the result
of relaxation of purifying selection and could be ascribed to cave habit of this genus. In contrast to previous hypotheses,
both cave and surface lineages exhibited a similar rate of molecular evolution, so the RH1 of cave species may still be functional, although these species were highly adapted to cave environment. Two amino acid substitutions
(D83G and E122V) that were not reported before were found, which may be useful for site-directed mutagenesis in the future.
Hydrobiologia 04/2009; 624(1):139-149. · 1.78 Impact Factor
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ABSTRACT: Wang W., Dai X., Li Z., Meng Q. (2009): Tissue distribution and elimination of florfenicol in topmouth culter (Culter alburnus) after oral administration. Czech J. Food Sci., 27: 216–221. The serum concentration and muscle residue of florfenicol following oral administration were evaluated in topmouth culter (Culter alburnus) under field conditions. Fishes were cultured in tanks and fed a commercial medicated diet containing 2000 mg/kg florfenicol in a single dose. The sampling was conducted at different intervals (0, 0.5, h) after the cessation of medication. Drug analysis was carried out by HPLC. The maximum concentration (C max) of 10.8 μg/g in serum and 4.2 μg/g in muscle were reached at the same time (t max) of 8 hours. If the EU MRL value of 1.0 μg/g is quoted for the topmouth culter tissue, the extrapolation of the data indicated that it will be passed after a 40.6 h withdrawal period for florfenicol in topmouth culter muscle.
Czech J. Food Sci. 01/2009; 27:316-221.
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Ke Huang,
Wei Tang,
Zhao Xu, Zhiqiang Li,
Zhangdong He,
Ruqi Tang,
Ronglin Che,
Yifeng Xu,
Xingwang Li,
Guoyin Feng,
Lin He,
Yongyong Shi
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ABSTRACT: Schizophrenia is a chronic psychiatric disorder with a strong genetic component. Several recent published studies have reported that the mRNA expression level of quaking homolog, KH domain RNA binding (QKI) is down regulated in individuals diagnosed with schizophrenia.
We were interested in the genetic variants around the promoter region of QKI and selected seven variants in this region, namely rs4263561, rs3904720, rs387504, rs3763197, rs7772756, rs7758706 and rs4709716. For the study we recruited 288 individuals diagnosed with schizophrenia and 288 control subjects. All the recruits were from Shanghai and were Han Chinese in origin.
No individual SNP nor any haplotype was found to be associated with schizophrenia.
These results suggest that the variants within the promoter region of QKI gene are unlikely to play a major role in susceptibility to schizophrenia in the Chinese population.
Progress in Neuro-Psychopharmacology and Biological Psychiatry 11/2008; 33(1):33-6. · 3.25 Impact Factor
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ABSTRACT: ObjectiveTo study the expressions of FAK and Pyk2 in human astrocytic tumors and their relationship with angiogenesis.
MethodsThe S-P immunohistochemical method was used to measure the expressions of FAK, Pyk2 and VEGF proteins in 58 human brain astrocytic
tumors, and microvessel density (MVD) was detected by CD31 staining.
ResultsIn astrocytic tumors with I, II, III and IV grades, the positive rates of FAK were 20.00%, 26.67%, 44.44% and 50.00%, respectively,
those of Pyk2 were 40.00%, 60.00%, 77.78% and 85.00%, respectively. FAK and Pyk2 expressions, especially Pyk2, correlated
positively with VEGF expression and MVD.
ConclusionFAK and Pyk2 plays the important role in astrocytic tumor angiogenesis through regulation to VEGF.
The Chinese-German Journal of Clinical Oncology 10/2008; 7(11):658-660.
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ABSTRACT: The neural cell adhesion molecule 1(NCAM1, aliases NCAM and CD56) is a cell-surface molecule which makes homophilic adhesion between neural cells involved in cell migration, axon outgrowth and synaptic plasticity. Recent studies reported that NCAM1 might act as a candidate schizophrenia susceptibility gene.
We genotyped five SNPs (rs1943620, rs1836796, rs1821693, rs686050, rs584427) within the NCAM1 gene and conducted a case-control study in 288 schizophrenic patients and 288 healthy subjects in the Chinese Han population. We compared allele and genotype frequencies and haplotype distributions between cases and controls.
No significant differences in allele and genotype frequencies were found for each single SNP between schizophrenic patients and healthy subjects. Moreover, there were no significant differences in haplotype distributions between cases and controls (global chi2=1.318, P=0.725, df=3).
Our study suggests that the five SNPs within NCAM1 gene we studied may not play a major role in the schizophrenia susceptibility in the Chinese Han population.
Progress in Neuro-Psychopharmacology and Biological Psychiatry 07/2008; 32(7):1633-6. · 3.25 Impact Factor
