-
[show abstract]
[hide abstract]
ABSTRACT: OBJECTIVE: To evaluate the relationship between bacterial biofilm (BBF) and chronic rhinosinusitis (CRS). METHODS: The database on line was searched to collect the studies on BBF and CRS. The method of meta analysis was used to analyze the data of suitable studies. RESULTS: Fourteen studies were included. System evaluation indicated that the BBF detection rate in CRS group was significantly higher than that in the control group (OR = 17.01, P < 0.01), and the nasal surgery's rate of BBF positive group was significantly higher than the negative group (OR = 3.99, P < 0.01). Preoperative Lund-Kennedy endoscopic score, Lund-MacKay CT score, symptom severity score, postoperative Lund-Kennedy score after six months showed no difference between BBF positive group and negative group. CONCLUSION: The presence of BBF is related to the pathogenesis of CRS and the history of nasal surgery.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 12/2012; 47(12):1021-1026.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the distribution of common allergens in patients with allergic rhinitis (AR) from 2006 to 2010 in Wuhan area, and provide the objective evidence for the prevention and treatment of AR.
The medical records of skin prick test (SPT) performed on 2707 AR patients from 2006 to 2010 were restrospectively analysed, and the positive rate of different allergens and changing trends in this time were compared. SPSS 17.0 software was used to analyse the data.
There were significant differences among the Dermatophagoides pteronyssinus positive rate (χ(2) = 12.11, P < 0.05) and Dermatophagoides farinae positive rate (χ(2) = 11.11, P < 0.05) in the past 5 years. Meanwhile, there was an upward trend in the positive rate of dust mite, which the positive rate of Dermatophagoides pteronyssinus increased from 84.5% in 2006 to 90.5% in 2010 (χ(2) = 6.88, P < 0.05), positive rate of Dermatophagoides farinae increased from 81.5% in 2006 to 89.0% in 2010 (χ(2) = 9.68, P < 0.05); There were significant differences among the Mugwort and Ragweed positive rate of 5 years (χ(2) = 194.10, P < 0.05; χ(2) = 67.06, P < 0.05). There were significant differences among the mold I and mold II positive rate of 5 years between (χ(2) = 18.95, P < 0.05; χ(2) = 36.62, P < 0.05). Meanwhile, there was an upward trend in the positive rate of mold and fluctuant trend in the positive rate of spring-pollen.
Nearly five years, dust mites is still the most common allergens in AR patients, presenting upward trend; the positive rate of mold presenting upward trend; the positive rate of wormwood and guineawood presenting downward trend; the positive rate of pollen presenting fluctuant trend.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 08/2012; 47(8):680-2.
-
[show abstract]
[hide abstract]
ABSTRACT: The Notch signaling pathway plays an important role in the proliferation and differentiation of cells. Although recent studies have shown that Notch plays a role in the mechanisms of cisplatin resistance, the mechanism by which Notch plays roles in intrinsic or acquired cisplatin resistance remains unclear. In the present study, poorly differentiated nasopharyngeal carcinoma cells were treated with a γ-secretase inhibitor (DAPT), which led to a decrease in the Notch intracellular domain and inhibition of Notch signaling. Treatment was not sufficient to induce pronounced apoptosis of CNE-2 cells, but did result in the down-regulation of the P-glycoprotein and ERCC1 protein. In contrast, the combined treatment of DAPT and cisplatin induced substantial cell apoptosis compared to cisplatin treatment alone.
Experimental and therapeutic medicine 02/2012; 3(2):357-361.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the positive rates of some allergens in different age groups of allergic rhinitis patients, and to analyze the relationship between serum specific immunoglobulin E(sIgE) and total immunoglobulin E(IgE).
Six hundred and fifty-three patients with allergic rhinitis, tested by AllergyScreen system, were enrolled in our research.
The positive rate of sIgE in all patients was 85.5%. The positive rate of dust mite was the highest with the result of 76.3%, and the positive rate of feline or dogs hair was the lowest of 20.3%. The statistical significance of different positive rate of some allergens were found in the different age groups. All the patients were divided into two groups, one was younger than 18 years old group, which including 18 years old and another was older than 18 years old group. Except Mx, the positive rate of other allergens existed statistical difference. The level of serum total IgE could not reflect the positive rate of sIgE completely.
It is an effective and reliable method of the diagnosis of allergic rhinitis to combine the analysis of total serum IgE and sIgE levels with patients clinical manifestations. Different strategies should be employed for the prevention and treatment of allergic rhinitis according to their ages.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2011; 25(8):339-41, 346.
-
[show abstract]
[hide abstract]
ABSTRACT: To compare the therapeutic effect of radiofrequency ablation and focused ultrasound in treatment of allergic rhinitis which was refractory to medical therapy, and to explore the reliable and safe surgical treatment.
Eighty-six perennial allergic rhinitis patients were divided randomly into two group. The radiofrequency ablation group included 40 patients and focused ultrasound group included 46 patients. A standard 0 to 10 visual analog scale (VAS) was used to assess the allergic symptoms therapeutic effect before and one year after treatment.
Allergic symptoms were improved in both two groups one year after treatment. The total VAS score in radiofrequency ablation group decreased from 31.35 +/- 6.16 to 14.16 +/- 4.83 and the total effective power of this group was 87.5% at the twelfth month after surgical. While the total VAS score in focused ultrasound group decreased from 32.86 +/- 5.45 to 21.91 +/- 8.58 and total effective power was 69.6% (all P < 0.01).
This study had demonstrated that the radiofrequency ablation and focused ultrasound appears to be an effective and safe tool for treating allergic rhinitis which was refractory to medical therapy. Compared to focused ultrasound, the radiofrequency ablation seemed to have a longer time therapeutical effect. While the focused ultrasound was more safe and little trauma, which made it possible to the repeated application.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2011; 25(8):349-51.
-
[show abstract]
[hide abstract]
ABSTRACT: To evaluate symptoms and quality of life in children with allergic rhinitis, and to evaluate the correlations among the skin prick tests results, symptoms and quality of life.
Visual analog scale (VAS) was used to assess the symptoms severity, rhinoconjunctivitis quality of life questionnaire (RQLQ) was used to assess quality of life, the skin prick tests results were recorded, and statistics analysis was carried out among them.
(1) There were significant differences among the five aspects of RQLQ (F = 32.03, P < 0.01), nasal symptoms aspect was the most affected aspect of quality of life, moreover, there were significant differences among all the items of RQLQ (F = 7.35, P < 0.01), the scores of nasal blockage, sneezing and rhinorrhea were the highest, there were no significant differences among them. (2) VAS was significantly correlated with RQLQ, moreover, the scores of unable to get to sleep and embarrassed by symptoms were significantly correlated with rhinorrhea VAS scores (r = 0.230, P < 0.05; r = 0.325, P < 0.01), the score of wake up during the night was significantly correlated with nasal itching and sneezing VAS scores (r = 0.385, P < 0.01; r = 0.231, P < 0.05), the score of can't concentrate was significantly correlated with the scores of unable to get to sleep and wake up during the night (r = 0.316, P < 0.01; r = 0.525, P < 0.01), the score of irritable was significantly correlated with the scores of unable to get to sleep (r = 0.243, P < 0.05). (3) The results of the skin prick tests were related with VAS and RQLQ.
Nasal symptoms severity may significantly correlated with the quality of life. Rhinorrhea, nasal itching and sneezing may be the main factors affecting the quality of sleep, while the quality of sleep may affect non-hay-fever symptoms and emotions, rhinorrhea may be the main factor affecting embarrassed by symptoms. The allergen level was related with the symptoms severity and quality of life.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 12/2010; 24(23):1071-3, 1076.
-
[show abstract]
[hide abstract]
ABSTRACT: The development of allergic rhinitis is considered to be determined by the interaction between genetic and environmental factors. Surfactant protein A (SP-A), a member of the collectin family of proteins, plays an important role in immune regulation. The purpose of this study was to investigate the association between SP-A polymorphisms and allergic rhinitis. We conducted a case-control association study on a Chinese Han population, comprising 216 adult individuals with AR and 84 healthy controls. A total of 9 single-nucleotide polymorphisms (SNPs) mapped to the SP-A were genotyped using PCR-based molecular identification methods. The frequency of A allele at amino acid 223 in the patient group was significantly higher than that in the control group after correcting for multiple testing (P = 0.006). The 1A(2) allele haplotype in SFTPA2 was associated with decreased risk for allergic rhinitis, after applying Bonferroni corrections (P = 0.003). However, genetic variants of the SFTPA1 genes were not found to be associated with AR. In addition, no significant associations were established between any of the 9 SFTPA gene polymorphisms and the skin-prick test responses (P > 0.05). Further, no association was established between the 9 SNP loci and the levels of total serum immunoglobulin E (IgE) (P > 0.05). These results indicate that the gene polymorphism at the residue 223 in the carbohydrate recognition domain of SFTPA2 may be a genetic marker for the development of AR in the adult Chinese Han population.
Molecular Biology Reports 10/2010; 38(3):1475-82. · 2.93 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To establish the activation of NF-kappaB in middle ear cholesteatoma, discuss the relationship of NF-kappaB and the gene expression of IL-6 and explore the pathogenesis of middle ear cholesteatoma.
Ten cases of cholesteatoma and 6 cases of normal external meatal skin were obtained from middle ear surgery. The NF-kappaB DNA binding activity and the mRNA level of IL-6 in these two kinds of tissues were detected by electrophoretic motility shift assay (EMSA) and Reverse transcription polymerase chain reaction (RT-PCR) respectively. The relationship of NF-kappaB DNA binding activity and the mRNA level of IL-6 were analyzed.
The NF-kappaB DNA binding activities of cholesteatoma [(15.9 +/- 8.2)%] were higher than those in normal skin [(1.36 +/- 0.94)%, t = 3.502, P < 0.05]. The expression of IL-6 mRNA was increased significantly in patients with cholesteatoma, as compared with that in the control specimens (t = 2.166, P < 0.05) and had a significant positive correlation with NF-kappaB DNA binding activity (r = 0.752, P < 0.05).
The IL-6 mRNA expression in cholesteatoma is closely related with the activity of NF-kappaB. It is tempting to speculate that NF-kappaB play a key role in the activation of cytokine in cholesteatoma.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 03/2009; 44(3):192-6.
-
[show abstract]
[hide abstract]
ABSTRACT: Telomerase activity is mainly regulated by the human telomerase reverse transcriptase (hTERT) gene. Our objective was to investigate the effect of short hairpin RNA (shRNA) directed against hTERT mRNA on telomerase activity in laryngeal cancer cells (Hep-2), nasopharyngeal carcinoma cells (NEC), and human bone marrow mesenchyme stem cells (hMSCs). Short hairpin RNA expression vectors targeting the messenger RNA of hTERT were constructed. Cells were treated with shRNA expression vectors directed against hTERT mRNA and control vectors that included mismatched shRNA. We found that treatment of special shRNA expression vectors induced significantly decrease in hTERT expression, telomerase activity, and cell viability in Hep-2 and NEC cells. In contrast, the shRNA control showed none of these effects. And none of these effects appeared in hMSCs cells. Our results suggest that shRNA against hTERT mRNA inhibits telomerase activity and cell viability through suppression of the hTERT expression in cancer cells. And this treatment has no side effect on healthy cells lack of telomerase activity. RNA interfering technology may be a promising strategy for the treatment of cancers.
Cancer Investigation 01/2008; 25(8):691-8. · 1.85 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To explore the etiology of paranasal sinus fungus ball and evaluate the curative effect of endoscopic sinus surgery on paranasal sinus fungus ball.
The clinical and follow-up data of 55 patients with paranasal sinus fungus ball were retrospectively analyzed.
All patients were cured by endoscopic sinus surgery, no complications were observed. Following up for 6-48 months, two cases were recurred.
Anatomic abnormalities of the paranasal sinuses and wound in the mucosa are the main pathogenesis of paranasal sinus fungus ball. Endoscopic sinus surgery is an effective treatment of paranasal sinus fungus ball. Relapse rate is related with regular irrigation after operation.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 01/2008; 21(23):1075-7.
-
[show abstract]
[hide abstract]
ABSTRACT: To discuss the complication of common anatomic variant of the middle turbinate-concha bullosa.
We present a 35 years old man with inner canthus proptosis and orbital pain who was diagnosed to be a pyocele originated from concha bullosa by CT and operation.
Concha bullosa can develop to a pyocele. Direct extension of the mass from the nose into the orbit may occur. Good therapeutic effect was obtained by endoscopic operation.
Concha bullosa can result in obstruction of middle meatus and lead to sinusitis. Polyps or mucocele may also occur to Concha bullosa itself. Pyocele of concha bullosa can develop to such a massive extent that it leads to orbital complication. Endoscopic operation is best treatment to this disease.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 01/2008; 21(23):1085-6.
-
[show abstract]
[hide abstract]
ABSTRACT: To explore the expression and activation of NF-kappaB in middle ear cholesteatoma.
The protein expression of NF-kappaB p65 in 21 middle ear cholesteatoma tissues and 8 normal external ear canal skin obtained in middle ear surgery were examined by immunohistochemistry; NF-kappaB DNA binding activity in these two kinds of tissues were also detected by electrophoretic motility shift assay (EMSA). The influence of cholesteatoma debris on the NF-kappaB DNA binding activity of HaCat cell were further analyzed.
All epithelial cell of cholesteatoma revealed a relatively abundant plasma expression of NF-kappaB p65 protein, among which 12 cases showed nuclear positive expression. In contrast,the normal skin epithelium only revealed a sparse plasma distribution of NF-kappaB protein. The levels of NF-kappaB p65 protein expression in the epithelium of middle ear cholesteatoma tissue and normal skin were 0.168 +/- 0.051, 0.088 +/- 0.019 (t = 4.211, P < 0.01), respectively. The NF-kappaB DNA binding activities of cholesteatoma [(16.5 +/- 10.1)%] were also higher than those in normal skin [(1.38 +/- 1.24)%, t = 3. 600, P = 0.014]. The NF-kappaB DNA binding activity of HaCat cell increased when exposed to cholesteatoma debris in a dose dependent manner.
NF-kappaB might be an important factor which was involved in the occurrence and development of cholesteatoma.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 06/2006; 41(6):455-9.
-
[show abstract]
[hide abstract]
ABSTRACT: To evaluate the clinical manifestations of laryngeal tuberculosis and its laryngoscopic characteristics.
Clinical analysis of 36 laryngeal tuberculosis cases confirmed pathologically was carried out retrospectively.
The patients' ages ranged from 19 to 78 years old (median 39.5 years old). The male to female ratio was 1.8 to 1. The most frequent chief complaint was hoarseness (83.3%). The true vocal cord and the false vocal cord were usually found involved by fibrolaryngoscope observations. The lesions appearances were mainly the proliferation such as nodules or granules. The laryngostroboscopy demonstrated that the wave of the mucosa and vibration of the involved vocal cords were markedly reduced or disappeared. Among 24 patients with multiple lesions in the larynx, 19 (79.2%) had active pulmonary tuberculosis,while 9 (75.0%) had normal lung status among 12 patients with single lesions in the throat. All the patients received a full course of antituberculosis treatment. The results of the antituberculous medication was satisfactory in all 26 cases patients who can be followed up, while 10 cases were lost for the follow-up.
The local clinical manifestations of laryngeal tuberculosis is nonspecific, while the whole manifestation is not apparent. The lesions commonly involve the true vocal cord and the false vocal cord. It can even occur without pulmonary tuberculosis.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 05/2006; 41(4):247-50.
-
[show abstract]
[hide abstract]
ABSTRACT: To observe the incidence rate of chronic sinusitis in the patients with perennial allergic rhinitis, and explore the possible etiology of sinusitis.
Fifty-seven patients were examined with endoscope and CT scan.
Of the 57 patients, 18 (32%) patients had chronic sinusitis, among which 17 patients had different kinds and different extents of anatomy abnormalities of nose cavity including deviation of nasal septum, concha bullosa, hypertrophy of uncinate process, paradoxical curvature of middle turbinate, etc. Thirty-nine patients didn't complicated with chronic sinusitis, among which only 7 patients had anatomy abnormalities of nose cavity.
Anatomy abnormalities of nose cavity may be the basic etiology of sinusitis in patients with perennial allergic rhinitis; The edema of nasal mucous and the change of nasal discharge caused by perennial allergic rhinitis may be the triggering factor of chronic sinusitis. Both of these two factors are involved in the pathogenesis of sinusitis.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 10/2005; 19(17):790-1.
-
[show abstract]
[hide abstract]
ABSTRACT: To research the pathogenic microorganism characters of chronic pharyngitis.
Samples taken from pharyngeal swab from patient with chronic pharyngitis were routinely cultured; mp-DNA were detected by polymerase chain reaction (PCR).
Of all the cases, 5 patients were DNA(+), among which 3 patients got obvious effect after 2 weeks treatment. The bacterial abnormally was present in 15 patients (31.5%), among which 6 patients got obvious effect and 2 patients got no effect after 2 weeks treatment.
The pathogenic abnormally may be present in some patients with chronic pharyngitis, most of which appeared to be an internal disorder of the proper microflores. C. pneumoniae may be an aetiological agent for chronic pharyngitis. An appropriate antimicrobial treatment should be considered in some cases.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 04/2004; 18(3):136-8.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the mode of expression and distribution of transforming growth factor alpha in middle ear cholesteatoma.
The specimens from the cholesteatoma tissue of 32 cases and the normal external ear skin of 10 cases were examined by immunohistochemical S-P method and computer image analysis.
In cholesteatoma epithelium staining for TGF alpha was consistently stronger than that in normal epithelium. The mean absorbance of the two epithelial tissue is 2.431 +/- 0.587 and 1.463 +/- 0.147 respectively, revealing a significant difference. Some immune cells infiltrating the stroma of cholesteatoma also stained positively for TGF alpha.
These data suggested that an autocrine and paracrine stimulation of the squamous epithelium of cholesteatoma by TGF might contribute to its hyperproliferation.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 02/2003; 17(1):32-4.
