Yoshihiro Wakabayashi

Publications (2)1.79 Total impact

• Article: Appearance of LFA-1 in the initial stage of synaptogenesis of developing hippocampal neurons.

  Yoshihiro Wakabayashi, Atsushi Tsujimura, Ken-Ichi Matsuda, Norio Yoshimura, Mitsuhiro Kawata
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  ABSTRACT: In the present study, we found that leucocyte function-associated antigen-1 (LFA-1), and integrin (heterodimer complex of CD11a and CD18), which are abundant in immunological synapse, were expressed in developing hippocampal neurons. The expression of LFA-1 in hippocampal neurons was in the period of synaptogenesis, and synaptogenesis was inhibited by the blocking antibodies of anti-CD11a or anti-CD18 in vitro. Since it is known that LFA-1 has an important role in the immunological response, especially in immunological synapse, LFA-1 is considered to have an important role in neuronal synapse and is highly involved in synaptogenesis in the early developmental stage in vitro. In vivo, we also confirmed that CD18 was expressed in hippocampus in the early developmental stage. Telencephalin, which is a candidate for postsynaptic elements to contact LFA-1, was precisely opposed to CD18-positive structures in presynaptic elements, and telencephalin was considered to be involved in synaptogenesis. The present study showed that 17beta-estradiol of steroid hormones, which are well known to have various effects on hippocampal neurons, has a significant influence on the presynaptic expression of CD18 in synaptogenesis and inhibited synaptogenesis in the early developmental stage in vitro. These results suggest that LFA-1 plays some mechanisms in synaptic contacts and synaptogenesis of hippocampal neurons.
  Archives of Histology and Cytology 04/2008; 71(1):23-36. · 0.57 Impact Factor
• Article: Management and outcome of living kidney grafts with multiple arteries.

  Yayoi Kadotani, Masahiko Okamoto, Kiyokazu Akioka, Hidetaka Ushigome, Shiro Ogino, Shuji Nobori, Atsushi Higuchi, Yoshihiro Wakabayashi, Satoshi Kaihara, Norio Yoshimura
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  ABSTRACT: Kidney allografts with multiple renal arteries (MRAs) have been used with increasing frequency since the advent of laparoscopic live donor nephrectomy. To determine if MRA grafts affect the short- and long-term outcomes of grafts and patients, we analyzed 340 grafts procured by open nephrectomy. We divided the graft recipients into five groups according to the methods used for vascular reconstruction. We compared patient and graft survival, serum creatinine levels, total (rewarm) ischemic times (TIT), incidence of acute tubular necrosis (ATN), need for antihypertensive drugs, incidence of acute rejection episodes, and vascular and urologic complications, between the MRA group and a control group of patients with single-artery renal grafts. In patients who underwent multiple anastomoses in situ, prolonged TIT resulted in an increased incidence of ATN, but there was no significant difference between the MRA groups and the control group (P = 0.45). The incidence of vascular complications was higher in the MRA groups (P < 0.01), but there were no significant differences in the other variables among the groups. Multiple renal artery grafts procured by open nephrectomy can be transplanted as successfully as those with single arteries, by using meticulous suturing techniques.
  Surgery Today 01/2005; 35(6):459-66. · 1.22 Impact Factor