Z Gao

Huazhong University of Science and Technology, Wu-han-shih, Hubei, China

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Publications (3)9.1 Total impact

  • H Xu, K Huang, Q Gao, Z Gao, X Han
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    ABSTRACT: The contents of mineral elements and amino acids in the hydrolysate of the traditional Chinese mineral medicine nacre have been determined. It has long since been proved by the practice of doctors of traditional Chinese medicine that pearl can be used to treat eye diseases. Based on such an understanding, a study is made of the influence of the said medicine on the incidence of myopia. First a form-sense-deprived model (FDM) for chicks is developed and the effect of the said medicine on the elongation of axis oculi is determined with an Abbe's comparator and an A-mode ultrasound instrument. The activity of superoxide dismutase (SOD), nitric oxide synthetase (NOS), and the content of nitric oxide (NO) in the retino-pigmental epithelium choroid homogenate are also analysed. The role of the said traditional Chinese mineral medicine in preventing and treating myopia is explained with respect to the above findings. The results obtained will provide a basis for using nacre, a traditional Chinese mineral medicine, to prevent and treat myopia.
    Pharmacological Research 08/2001; 44(1):1-6. DOI:10.1006/phrs.2000.0780 · 3.98 Impact Factor
  • Z Gao, K Huang, H Xu
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    ABSTRACT: Oxidative stress plays an important role in the pathological process of neurodegenerative diseases. The effects of four major flavonoids present in Scutellaria baicalensis Georgi on hydrogen peroxide-induced neuronal cell damage are studied in this paper. When human neuroblastoma SH-SY5Y cells were incubated in Hanks' solution with the addition of 400 microM hydrogen peroxide for 2 h, the viability of cells was decreased remarkably, while the cell lipid peroxidation and the percentage of lactose dehydrogenase released into the culture medium was significantly increased. Addition of 10 microM of baicalein and baicalin significantly attenuated the cellular injury induced by hydrogen peroxide, while the effect of wogonin was marginal and wogonoside showed no effect at the tested concentration. In a separate experiment, 10 microM of baicalein and baicalin also antagonized the intracellular free-calcium concentration ([Ca2+]i) increase caused by 1 mM hydrogen peroxide. The effects of baicalein in both experiments were similar to those of quercetin, a well-studied antioxidant flavonoid. These results demonstrated the protective effects of flavonoids originating from Scutellaria baicalensis Georgi on the oxidative injury of neuronal cells.
    Pharmacological Research 03/2001; 43(2):173-8. DOI:10.1006/phrs.2000.0761 · 3.98 Impact Factor
  • X. Yang, H. Tue, Z. Gao, Y. Yi, H. Xu
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    ABSTRACT: Considerable evidence indicates that the formation of peroxynitrite (ONOO−) with superoxide anion (O2−⊙ot) may be involved in the neuronal toxicity of NO. Here, the effects of ONOO− on intracellular free calcium concentration ([Ca2+]i) in single MN9D cells was studied by the Fura-2 microfluorometric technique. The results showed that [Ca2+]i was increased dose-dependently with the addition of ONOO− (0–40 μmol/l) after 5 s and then decreased rapidly back to the basal level after ONOO− was removed. [Ca2+]i response to ONOO− can be blocked by removing Ca2+ from the bath or adding L-type calcium channel antagonist nifedipine (10 μmol/l) to the bath. [Ca2+]i transients by ONOO− were substantially inhibited by dithiothreitol (DTT), which indicated ONOO− may alter the L-type calcium channel on neuronal cell by thiol oxidation. To elucidate the mechanism of ONOO− on [Ca2+]i, the electron spin resonance spin-labeling technique was used to study the effects of ONOO− on the membrane fluidity and the membrane protein conformation on freshly dissociated neurons. The results indicate that ONOO− decreases membrane fluidity both near the surface and deep in the membrane and affects protein conformation. The fact that DTT effectively inhibits the deterioration supports the conclusion that the change of membrane fluidity and protein conformation is involved in [Ca2+]i overload in neuronal cells induced by ONOO−.
    Applied Magnetic Resonance 01/2000; 19(2):237-243. DOI:10.1007/BF03162278 · 1.15 Impact Factor