Ying-Jie Wei

China Pharmaceutical University, Nan-ching-hsü, Jiangxi Sheng, China

Are you Ying-Jie Wei?

Claim your profile

Publications (8)14.14 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Caudatin-2,6-dideoxy-3-O-methy-β-D-cymaropyranoside (CDMC), the C-21 steroidal glycoside recently extracted from the traditional Chinese medicinal plant, the root of Cynanchum auriculatum Royle ex Wight (Asclepiadaceae), has been shown to possess potent antitumor properties. However, the bioactivities of CDMC are still largely unknown, especially the antitumor effect and its mechanism. This study investigated the CDMC antitumor effects on human hepatoma cell line SMMC7721 cells by analysis of cell viability, cell cycle phases and apoptosis. The results showed that CDMC inhibited the growth of SMMC7721 cells in a time- and dose-dependent manner and resulted in cell cycle arrest in G(0)/G(1) phase. Furthermore, CDMC induced SMMC7721 cell apoptosis rather than necrosis through caspase 3 activation, and a caspase 3 inhibitor, Ac-DEVD-CHO, could attenuate the apoptosis induced by CDMC. The results suggested that the anticancer activity of CDMC could be attributed partially to its inhibition of cell proliferation and induction of apoptosis associated with caspase 3 activation.
    Phytotherapy Research 10/2010; 25(5):631-7. · 2.07 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A method for predicting bioactive candidates in herbal medicines (HMs) was developed using four types of target cell extraction followed by high performance liquid chromatography coupled with diode array detection-mass spectrometry (HPLC-DAD-MS) analysis. Through the proposed method, some potential bioactive components in Radix Salviae miltiorrhizae, a widely used HM for the treatment of cardiovascular diseases, were screened in view of interacting with endothelial cells, myocardial cells, blood platelets and red cells. Specifically, eleven compounds were detected in the desorption eluate of endothelial cells, nine in myocardial cells, fifteen in blood platelets and five in red cells. Totally, sixteen compounds were detected and fourteen of them were identified qualitatively in terms of their MS spectra and in comparison with some of the reference compounds. The results indicate that the proposed method may be applied to prediction of the bioactive multi-compounds in HMs.
    Biological & Pharmaceutical Bulletin 04/2008; 31(3):501-6. · 1.85 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A high-performance liquid chromatographic (HPLC) method coupled with ultraviolet (UV) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) was established for simultaneous qualitative and quantitative determination of nine phenolic acids and six diterpenoids in Radix et Rhizoma Salviae Miltiorrhizae (RRSM). The optimal chromatographic conditions were achieved on a Zorbax C(18) column by gradient elution with 0.1% (v/v) aqueous formic acid and acetonitrile as mobile phase at the flow rate of 1.0 mL/min. The detection wavelength at 281 nm was chosen to determine the 15 bioactive components, namely danshensu (1), protocatechuic acid (2), protocatechuic aldehyde (3), caffeic acid (4), rosmarinic acid (5), lithospermic acid (6), salvianolic acid B (7), salvianolic acid A (8), salvianolic acid C (9); dihydrotanshinone I (10), cryptotanshinone (11), tanshinone I (12), methylene tanshiqunone (13), tanshinone IIA (14) and miltirone (15). Additionally, LC-ESI-TOF/MS was used to make definite identification of the constituents in samples in comparison with those reference compounds. The validation of the method included tests of linearity, sensitivity, repeatability, stability and recovery. The proposed method was successfully applied to quantify the 15 components in 21 samples; significant variations were demonstrated in the contents of the samples from diverse species and origins. The developed method could be used to effectively and comprehensively evaluate the quality of RRSM for its clinical safety and efficacy.
    Biomedical Chromatography 03/2008; 22(2):164-72. · 1.95 Impact Factor
  • Biological & Pharmaceutical Bulletin - BIOL PHARM BULL. 01/2008; 31(3):501-506.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A herb-combined prescription, mainly derived from roots of Salvia miltiorrhiza and Panax notoginseng, has been widely used for improving coronary or cerebral circulation in China as well as in Western countries. Multiple commercially available preparations, known as Fufang Danshen preparations (FDPs), produced by various manufacturers with the raw materials from different sources, pose a serious challenge to the quality control of this herb medicine. Previous pharmacological studies identify three types of bioactive components correlated with the clinical effect of those herb preparations. Those mainly include four phenolic acids, four saponins and four diterpenoid quinones. In this report, by using high performance liquid chromatography (HPLC) coupled with diode array and evaporative light scattering detectors (DAD-ELSD), we developed an improved quality control method for those herb medicines. A simultaneous separation and quantification of the 12 components was performed on a C(18) column, in which the mobile phase consisted of (A) 0.1% aqueous formic acid and (B) acetonitrile using a gradient elution. The optimum detection wavelength was set at 281 nm, the drift tube temperature of ELSD was set at 113 degrees C, the nitrogen flow rate at 3.1L/min, and the gain=4. All calibration curves showed good linear regression (r(2)>0.9927) within test ranges. The method developed showed good precision and accuracy with overall intra- and inter-day variations of 0.64-4.79% and 0.69-4.96%, respectively, and the overall recoveries of 93.50-107.69% for the 12 compounds analyzed. This method was successfully applied to quantify the twelve components in ten commercial samples from three formulas by seven independent manufacturers. This readily available, low-cost and reliable HPLC-DAD-ELSD method improved the quality control of traditional Chinese medicinal preparations consisting of complex compounds with different structures such as FDPs.
    Journal of Pharmaceutical and Biomedical Analysis 12/2007; 45(5):775-84. · 2.95 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this research was to establish a simple, practical and efficient method for routine quantitative analysis of Erigeron breviscapus and its extract injection to control their qualities. The reversed phase high performance liquid chromatographic method was adopted to determine simultaneously the contents of two major classes of constituents namely phenolic acids and flavonoids, which were usually ignored in previous studies of E. breviscapus. Under the optimum conditions, three flavonoids including scutellarin, scutellarein and apigenin and four phenolic acids including caffeic acid, chlorogenic acid, 3,4-O-dicaffeoylquinic acid and 3,5-O-dicaffeoylquinic acid were successfully separated on a Zorbax SB-C18column (250×4.6mm I.D., 5.0μm particle size) at 25°C. Of the three flavonoids, scutellatin is a flavone glucuronide. The mobile phase was a mixture of acetonitrile and 1.0% (v/v) aqueous acetic acid employing gradient elution at a flow rate of 1.0mLmin−1 and the detection wavelength was set at 330nm. Regression equations revealed good linear relationship between the peak areas of the analytes and their concentrations (r 2 >0.9990). The relative standard deviations of retention time and peak area were less than 0.33 and 1.45%. The intra- and inter-day precisions as determined from sample solutions were below 1.66 and 2.35%. And the recoveries ranged from 96.5 to 101.8%. The proposed method has been successfully applied to the simultaneous quantification of two major classes of constituents in E. breviscapus and its extract injection for the first time.
    Chromatographia 08/2007; 66(5):395-399. · 1.44 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: High-performance liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) methods were developed for the analysis of chemical and metabolic components in traditional Chinese medicinal combined prescription containing Radix Salvia miltiorrhiza and Radix Panax notoginseng (commonly known as Fufang Danshen prescription, FDP). The HPLC experiments used a reversed-phase Zorbax C(18) column with the column temperature at 30 degrees C and a binary mobile phase system consisting of aqueous formic acid (0.1%, v/v) and acetonitrile using a gradient elution at the flow rate of 1.0 mL/min. The ESI-MS was operated with a single-quadrupole mass spectrometer in both negative and positive ion modes. 36 major chromatographic peaks of FDP, including 14 saponins, 13 phenolic acids and nine diterpenoid quinones were characterized by their MS spectra and in comparison with some of the reference standards. In addition, after oral administration of extraction of FDP, the rat's plasma, urine and feces were also analyzed; 53 metabolic components including 30 original components and 23 transformative components of FDP were detected, and possible metabolic pathways of some components in FDP were given. The analysis of chemical and metabolic components in FDP by HPLC-MS methods could be a useful means of identifying the multi-components of FDP and to hint at their possible metabolic mechanism of action in the body.
    Biomedical Chromatography 08/2007; 21(8):797-809. · 1.95 Impact Factor
  • Ying-Jie Wei, Song-Lin Li, Ping Li
    [Show abstract] [Hide abstract]
    ABSTRACT: A high-performance liquid chromatography method was established for simultaneously determining seven major components, i.e. protocatechuic aldehyde, notoginsenoside R(1), ginsenoside Rg(1), salvianolic acid B, ginsenoside Rb(1), cryptotanshinone and tanshinone IIA in Fufang Danshen tablet, a commonly used traditional Chinese medicinal combined prescription mainly derived from the roots of Salvia miltiorrhiza and Panax notoginseng. These seven compounds, belonging to the chemical types of phenolic acids, diterpenoid quinones and saponins, were simultaneously separated on Zorbax C(18) column (250 x 4.6 mm, 5.0 microm) with the column temperature at 30 degrees C. The mobile phase was composed of (A) aqueous phosphoric acid (0.1%, v/v) and (B) acetonitrile using a gradient elution of 7-17% B at 0-10 min, 17-20% B at 10-12 min, 20-21% B at 12-16 min, 21% B at 16-32 min, 21-29% B at 32-40 min, 29-35% B at 40-55 min, 35-65% B at 55-65 min and 65-80% B at 65-80 min; the flow rate was 1.0 mL/min. Detection wavelengths were set at 203 nm for notoginsenoside R(1), ginsenoside Rg(1) and ginsenoside Rb(1), 281 nm for protocatechuic aldehyde, salvianolic acid B, and 270 nm for cryptotanshinone and tanshinone IIA. All calibration curves showed good linear regression (r(2) > 0.9992) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 0.15-4.35 and 0.61-5.17% respectively, and overall recoveries of 94.8-102.1% for the seven compounds analyzed. The developed method has been successfully applied to simultaneous evaluation of the intrinsic quality of both Danshen and Sanqi in Fufang Danshen tablets from different pharmaceutical companies.
    Biomedical Chromatography 01/2007; 21(1):1-9. · 1.95 Impact Factor