W R Mayr

Medical University of Vienna, Wien, Vienna, Austria

Are you W R Mayr?

Claim your profile

Publications (320)1172.97 Total impact

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Eastern Austria is neighbouring regions with ongoing West Nile virus (WNV) transmissions. Three human WNV infections had been diagnosed during the past decade in Austria. The Austrian Red Cross Blood Service (ARC-BS) started a first voluntary screening for WNV in blood donors from Eastern Austria by Nucleic Acid Testing (NAT) in June 2014. This is also the most extensive WNV surveillance programme in humans in Austria so far. In August 2014, one autochthonous WNV infection was detected in a blood donor from Vienna. By now, one in 67,800 whole blood donations was found to be positive for WNV RNA. Copyright © 2015 Elsevier B.V. All rights reserved.
    Journal of Clinical Virology 01/2015; 64. DOI:10.1016/j.jcv.2015.01.003 · 3.47 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Eastern Austria is neighbouring regions with ongoing West Nile virus (WNV) transmissions. Three human WNV infections had been diagnosed during the past decade in Austria. The Austrian Red Cross Blood Service (ARC-BS) started a first voluntary screening for WNV in blood donors from Eastern Austria by Nucleic Acid Testing (NAT) in June 2014. This is also the most extensive WNV surveillance programme in humans in Austria so far. In August 2014, one autochthonous WNV infection was detected in a blood donor from Vienna. By now, one in 67,800 whole blood donations was found to be positive for WNV RNA.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background and Objectives Granulocyte-reactive antibodies can cause autoimmune and neonatal immune neutropenias as well as transfusion-related acute lung injury. The classical antibody-detection methods granulocyte aggregation test (GAT), granulocyte immunofluorescence test (GIFT) and monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) are time-consuming and technically challenging. In recent years, flow cytometric white blood cell immunofluorescence test (Flow-WIFT) and the microbeads assay LabScreen® Multi have emerged and are still subject of evaluation. These serological tests were compared on a screening and specification level.Materials and Methods For screening, the combination of GAT/GIFT was compared to Flow-WIFT testing 333 samples. Positive samples were further analysed with MAIGA and LabScreen® Multi.ResultsGranulocyte aggregation test/GIFT detected 77 positive samples, Flow-WIFT found 108 granulocyte-reactive samples. Six Samples were only positive in GAT/GIFT, and 37 samples were only positive in Flow-WIFT (κ = 0·682). Antibody specification with MAIGA and the microbeads assay confirmed granulocyte-reactivity in 83 cases with 70 matching results (κ = 0·742). However, out of six detected human neutrophil antigen (HNA) reactivities only two specificities matched in both assays.Conclusion Flow-WIFT may be a valuable addition to GIFT for granulocyte-reactive antibody screening. MAIGA remains the most reliable laboratory method for antibody specification.
    Vox Sanguinis 12/2014; 108(3). DOI:10.1111/vox.12227 · 3.30 Impact Factor
  • Source
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The DNA Commission of the International Society of Forensic Genetics (ISFG) regularly publishes guidelines and recommendations concerning the application of DNA polymorphisms to the question of human identification. Previous recommendations published in 2000 addressed the analysis and interpretation of mitochondrial DNA (mtDNA) in forensic casework. While the foundations set forth in the earlier recommendations still apply, new approaches to the quality control, alignment and nomenclature of mitochondrial sequences, as well as the establishment of mtDNA reference population databases, have been developed. Here, we describe these developments and discuss their application to both mtDNA casework and mtDNA reference population databasing applications. While the generation of mtDNA for forensic casework has always been guided by specific standards, it is now well-established that data of the same quality are required for the mtDNA reference population data used to assess the statistical weight of the evidence. As a result, we introduce guidelines regarding sequence generation, as well as quality control measures based on the known worldwide mtDNA phylogeny, that can be applied to ensure the highest quality population data possible. For both casework and reference population databasing applications, the alignment and nomenclature of haplotypes is revised here and the phylogenetic alignment proffered as acceptable standard. In addition, the interpretation of heteroplasmy in the forensic context is updated, and the utility of alignment-free database searches for unbiased probability estimates is highlighted. Finally, we discuss statistical issues and define minimal standards for mtDNA database searches.
    Forensic Science International: Genetics 07/2014; 13C:134-142. DOI:10.1016/j.fsigen.2014.07.010 · 3.20 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Granulocyte-reactive antibodies may cause transfusion-related acute lung injury (TRALI) and immune neutropenias. Risk factors for their acquisition other than previous alloexposition are largely unknown. In addition to the known association between human leucocyte antigen alloantibodies and red blood cell alloimmunization in selected cohorts of transfused patients, this study investigated a possible extension of this association to granulocyte-reactive antibodies in women with a history of pregnancy. The overall prevalence of granulocyte-reactive antibodies in 333 samples from women with a history of pregnancy (143 samples containing red cell alloantibodies) was 23·1%. The prevalence in the red cell-alloimmunized group (32·9%) was significantly higher than in controls (15·8%, P < 0·001). This could suggest that some individuals may be strong immunological responders, forming alloantibodies more readily than others.
    Vox Sanguinis 04/2014; 107(2). DOI:10.1111/vox.12152 · 3.30 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The significance of patient and donor ethnicity on risk of acute graft-versus-host disease (GVHD) and disease relapse after unrelated donor hematopoietic cell transplantation (HCT) is not known. A total of 4335 patient/donor pairs from the International Histocompatibility Working Group in HCT met the following three criteria: (1) HLA-A, B, C, DRB1 and DQB1 allele matched donor; (2) diagnosis of leukemia, and (3) non-T cell depleted GVHD prophylaxis. Post-transplant risks of acute GVHD and leukemia relapse were defined in Asian/Pacific Islander, Caucasian, African American, Hispanic, and Native American patients transplanted from donors with the same self-described background. Asian patients had a significantly lower incidence of acute GVHD (Japanese patients: 40.0% grades II-IV and 15.3% grades III-IV; non-Japanese Asian patients: 42.1% grades II-IV and 15.7% grades III-IV) compared to Caucasian patients (56.5% grades II-IV and 22.6% grades III-IV) (p< 0.001). The hazard ratio (HR) of acute GVHD for Caucasian patients was significantly higher than for Japanese patients. Unexpectedly, the HR of leukemia relapse in Caucasian patients with early disease status was also significantly higher than that in Japanese patients. These results provide a platform for future investigation into the genetic factors for unrelated donor HCT and clinical implications of diverse ethnic background.
    Biology of blood and marrow transplantation: journal of the American Society for Blood and Marrow Transplantation 06/2013; DOI:10.1016/j.bbmt.2013.05.020 · 3.15 Impact Factor
  • Vox Sanguinis 05/2013; 104(4). DOI:10.1111/vox.12003 · 3.30 Impact Factor
  • E.M. Dauber, W.R. Mayr
    Forensic Science International Genetics Supplement Series 01/2013; 4(1):e226-e227. DOI:10.1016/j.fsigss.2013.10.116
  • [Show abstract] [Hide abstract]
    ABSTRACT: DNA profiling of biological material from scenes of crimes is often complicated because the amount of DNA is limited and the quality of the DNA may be compromised. Furthermore, the sensitivity of STR typing kits has been continuously improved to detect low level DNA traces. This may lead to (1) partial DNA profiles and (2) detection of additional alleles. There are two key phenomena to consider: allelic or locus 'drop-out', i.e. 'missing' alleles at one or more genetic loci, while 'drop-in' may explain alleles in the DNA profile that are additional to the assumed main contributor(s). The drop-in phenomenon is restricted to 1 or 2 alleles per profile. If multiple alleles are observed at more than two loci then these are considered as alleles from an extra contributor and analysis can proceed as a mixture of two or more contributors. Here, we give recommendations on how to estimate probabilities considering drop-out, Pr(D), and drop-in, Pr(C). For reasons of clarity, we have deliberately restricted the current recommendations considering drop-out and/or drop-in at only one locus. Furthermore, we offer recommendations on how to use Pr(D) and Pr(C) with the likelihood ratio principles that are generally recommended by the International Society of Forensic Genetics (ISFG) as measure of the weight of the evidence in forensic genetics. Examples of calculations are included. An Excel spreadsheet is provided so that scientists and laboratories may explore the models and input their own data.
    Forensic Science International: Genetics 08/2012; 6(6):679-88. DOI:10.1016/j.fsigen.2012.06.002 · 3.86 Impact Factor
  • D V Devine, W R Mayr
    Vox Sanguinis 01/2012; 102(1):1. DOI:10.1111/j.1423-0410.2011.01571.x · 3.30 Impact Factor
  • E. M. Dauber, B. Glock, W. R. Mayr
    [Show abstract] [Hide abstract]
    ABSTRACT: An apparent paternal genetic incompatibility at the D2S1338 locus due to false homozygosity is described. It was disclosed after amplification with the AmpFlSTR® Identifiler® PCR Amplification Kit and reproducible with the genRES®MPX-4 DNA-Typing Kit. After application of an alternative primer set an allele *25 could be amplified, which restored Mendelian inheritance between father and child. Sequencing revealed a C>T transition at the fourth position from the 3′ end of the standard forward primer, which potentially caused the allelic loss with the primer sets in the commercial multiplex PCR kits.
    Forensic Science International Genetics Supplement Series 12/2011; DOI:10.1016/j.fsigss.2011.08.043
  • [Show abstract] [Hide abstract]
    ABSTRACT: One to two per cent of patients in need of red cell transfusion carry irregular antibodies to red blood cell (RBC) antigens and have to be supplied with specially selected blood units. To be able to respond to those requests, blood centres have to screen a significant number of donors for a variety of antigens serologically, which is a costly and through the shortage of reagents, also limited procedure. To make this procedure more efficient, the Austrian Red Cross has developed a genotyping assay as an alternative approach for high throughput RBC typing. A multiplex polymerase chain reaction (PCR) assay was designed for typing 35 RBC antigens in six reaction mixes. The assay includes both common as well as high-frequency-alleles: MNS1, MNS2, MNS3 and MNS4; LU1, LU2, LU8 and LU14; KEL1, KEL2, KEL3, KEL4, KEL6, KEL7, KEL11, KEL17 and KEL21; FY1, FY2, FYB(WK) and FY0 (FYB(ES)); JK1 and JK2; DI1, DI2, DI3 and DI4; YT1 and YT2; DO1 and DO2; CO1 and CO2; IN1 and IN2. The assay was validated using 370 selected serologically typed samples. Subsequently 6000 individuals were screened to identify high frequency antigen (HFA)-negative donors and to facilitate the search for compatible blood for alloimmunized patients. All controls showed complete concordance for the tested markers. The screening of 6000 donors revealed 57 new HFA-negative donors and the blood group database was extended by approximately 210,000 results. The study shows that in practice, this high-throughput genotyping assay is feasible, fast and provides reliable results. Compared to serological testing, this molecular approach is also very cost-efficient.
    Vox Sanguinis 11/2011; 102(3):234-42. DOI:10.1111/j.1423-0410.2011.01542.x · 3.30 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Apart from numerous single step mutations, four multi-step mutations and two double genetic inconsistencies have been observed among 50.796 allelic transfers at 23 STR-loci: ACTBP2 (=SE33), CD4, CSF1PO, F13A1, F13B, FES, FGA, vWA, TH01, TPOX, D2S1338, D3S1358, D5S818, D7S820, D8S1132, D8S1179, D12S391, D13S317, D16S539, D17S976, D18S51, D19S433, D21S11. These cases are described in the present study.
    24nd World Congress of the International Society for Forensic Genetics, Vienna, Austria; 09/2011
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Between 1988 and 2007, international searches for matched unrelated donors (MUDs) were performed for 1586 Austrian patients. Between 2004 and 2007, a MUD was identified for 76.7% of the patients. Between 1996 and 2003, a donor was identified for 71.3% of the patients, and between 1988 and 1995, only for 53.4% of the patients. Search times of successful searches decreased from 7.7 months in the first period to 1.7 months in the period from 2004 to 2007. However, transplants were not performed in all cases in which a donor was found: only in 61.6% of the patients between 2004 and 2007, in 53.4% between 1996 and 2003 and in 29.6% between 1988 and 1995. Multivariate analysis determined that having a common HLA type was the most important variable impacting on finding a MUD for a patient. Factors that most strongly influence a patient's access to transplant were the patient's European origin and a short time between diagnosis and start of donor search. The strongest factor for both finding a donor and being transplanted was a search being performed during more recent years: patients' chances increased from year to year.
    Bone marrow transplantation 04/2011; 47(2):172-80. DOI:10.1038/bmt.2011.67 · 3.00 Impact Factor
  • Source
    Vox Sanguinis 11/2010; 99(4):382-401. DOI:10.1111/j.1423-0410.2010.01379.x · 3.30 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The use of non-human DNA typing in forensic science investigations, and specifically that from animal DNA, is ever increasing. The term animal DNA in this document refers to animal species encountered in a forensic science examination but does not include human DNA. Non-human DNA may either be: the trade and possession of a species, or products derived from a species, which is contrary to legislation; as evidence where the crime is against a person or property; instances of animal cruelty; or where the animal is the offender. The first instance is addressed by determining the species present, and the other scenarios can often be addressed by assigning a DNA sample to a particular individual organism. Currently there is little standardization of methodologies used in the forensic analysis of animal DNA or in reporting styles. The recommendations in this document relate specifically to animal DNA that is integral to a forensic science investigation and are not relevant to the breeding of animals for commercial purposes. This DNA commission was formed out of discussions at the International Society for Forensic Genetics 23rd Congress in Buenos Aires to outline recommendations on the use of non-human DNA in a forensic science investigation. Due to the scope of non-human DNA typing that is possible, the remit of this commission is confined to animal DNA typing only.
    Forensic Science International: Genetics 11/2010; 5(5):501-5. DOI:10.1016/j.fsigen.2010.10.017 · 3.86 Impact Factor
  • Source
    W. R. Mayr
    [Show abstract] [Hide abstract]
    ABSTRACT: The current and future role of gene products of the HLA system in transfusion medicine, transplantation and cellular therapy is briefly discussed.
    ISBT Science Series 06/2010; 5(n1):176 - 178. DOI:10.1111/j.1751-2824.2010.01369.x
  • Source
    Tissue Antigens 04/2010; 75(4):291-455. DOI:10.1111/j.1399-0039.2010.01466.x · 2.35 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The WHO Nomenclature Committee for Factors of the HLA System met during the 15th International Histocompatibility and Immunogenetics Workshop in Buzios, Brazil in September 2008. This update is an extract of the main report that documents the additions and revisions to the nomenclature of human leukocyte antigen (HLA) specificities following the principles established in previous reports. Bone Marrow Transplantation (2010) 45, 846-848; doi: 10.1038/bmt.2010.79; published online 29 March 2010 HLA; Nomenclature; update
    Bone marrow transplantation 03/2010; 45(5):846-8. DOI:10.1038/bmt.2010.79 · 3.00 Impact Factor

Publication Stats

7k Citations
1,172.97 Total Impact Points


  • 1989–2014
    • Medical University of Vienna
      • Institute for Social Medicine
      Wien, Vienna, Austria
    • Freie Universität Berlin
      Berlín, Berlin, Germany
  • 1969–2014
    • University of Vienna
      • • Department of Internal Medicine III
      • • Department of Nuclear Medicine
      • • Institute of Immunology
      Wien, Vienna, Austria
  • 1997–2011
    • Österreichisches Rotes Kreuz
      Wien, Vienna, Austria
    • University of Münster
      • Institute of Legal Medicine
      Muenster, North Rhine-Westphalia, Germany
  • 2001–2010
    • Anthony Nolan Research Institute
      Londinium, England, United Kingdom
  • 1999
    • Oxford University Hospitals NHS Trust
      Oxford, England, United Kingdom
  • 1998
    • University of Oslo
      • Institute of Medical Informatics (IMI)
      Kristiania (historical), Oslo County, Norway
  • 1995
    • Vienna General Hospital
      Wien, Vienna, Austria
  • 1990–1995
    • Cancer Research UK
      Londinium, England, United Kingdom
  • 1991–1992
    • University Hospital RWTH Aachen
      Aachen, North Rhine-Westphalia, Germany
  • 1989–1992
    • RWTH Aachen University
      Aachen, North Rhine-Westphalia, Germany