Publications (3)6.06 Total impact
Article: [DNA methyltransferases: the role in regulation of gene expression and biological processes].[show abstract] [hide abstract]
ABSTRACT: Both hitone modification and DNA methylation remodulate chromatin structure and control gene expression or silence. As a main enzyme for DNA methylation, DNA methyltransferase (Dnmt) is not only associated with DNA methylation, but also links to many important biological activities, including cell proliferation, senescence and cancer development. This review focuses on structure, regulation and function in biological processes of Dnmt.Hereditas (Beijing) 11/2009; 31(11):1087-93.
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ABSTRACT: Histone modification as one of the key epigenetic regulation mechanisms plays critical roles in various biological processes, including regulation of chromatin structure dynamics and gene expression. Both histone methyltransferase and histone demethylases contribute to the establishment and maintenance of different histone methylation status. The 'effectors' which can recognize histone methylated sites build a relationship between these modifications and their down stream processes. This review summarized recent advances in histone methyltransferases and histone demethylases.Ai zheng = Aizheng = Chinese journal of cancer 11/2008; 27(10):1018-25.
Article: Histone deacetylase inhibitor depsipeptide activates silenced genes through decreasing both CpG and H3K9 methylation on the promoter.[show abstract] [hide abstract]
ABSTRACT: Histone deacetylase inhibitor (HDACi) has been shown to demethylate the mammalian genome, which further strengthens the concept that DNA methylation and histone modifications interact in regulation of gene expression. Here, we report that an HDAC inhibitor, depsipeptide, exhibited significant demethylating activity on the promoters of several genes, including p16, SALL3, and GATA4 in human lung cancer cell lines H719 and H23, colon cancer cell line HT-29, and pancreatic cancer cell line PANC1. Although expression of DNA methyltransferase 1 (DNMT1) was not affected by depsipeptide, a decrease in binding of DNMT1 to the promoter of these genes played a dominant role in depsipeptide-induced demethylation and reactivation. Depsipeptide also suppressed expression of histone methyltransferases G9A and SUV39H1, which in turn resulted in a decrease of di- and trimethylated H3K9 around these genes' promoter. Furthermore, both loading of heterochromatin-associated protein 1 (HP1alpha and HP1beta) to methylated H3K9 and binding of DNMT1 to these genes' promoter were significantly reduced in depsipeptide-treated cells. Similar DNA demethylation was induced by another HDAC inhibitor, apicidin, but not by trichostatin A. Our data describe a novel mechanism of HDACi-mediated DNA demethylation via suppression of histone methyltransferases and reduced recruitment of HP1 and DNMT1 to the genes' promoter.Molecular and cellular biology 06/2008; 28(10):3219-35. · 6.06 Impact Factor
Beijing, Beijing Shi, China
- • School of Basic Medical Science
- • Health Science Center
Peking University Health Science Center
Beijing, Beijing Shi, China
- Department of Biochemistry and Molecular Biology