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ABSTRACT: Perinatal infection is a major cause of neonatal neurologic morbidity. The goal of this study is to examine the effects of intrauterine infection on hippocampal neurogenesis and self-repair during early developmental stages. The animals were randomly divided into two groups: E. coli infected group and control group. Neurogenesis was examined by incorporation of BrdU, a marker of proliferating cells and their progeny. Rats were sacrificed on P3, P7, P14 and P28, and their brains were prepared for histological analysis of cell proliferation. To evaluate hippocampus neurogenesis, rats were sacrificed on P7 and P28, and their brains were prepared for evaluation of newly generated neural stem cells using double labeling of BrdU and Nestin, newly formed neurons using double labeling of BrdU and NeuN, and newly formed astrocytes using double labeling of BrdU and GFAP. In intrauterine E. coli infected group, there was significant increase in numbers of BrdU-labeled cells (about 2-fold at P7) than that of the control group (P<0.05). Confocal microscopy showed that there was a significant difference in BrdU/Nestin coexpression between the control and E. coli infected groups (P<0.01). Evaluation of the phenotype of the surviving cells showed that E. coli infected and control groups had a similar proportion of neuronal and glial differentiation. No significant difference was found in the percentage of newborn cells expressing neuronal and glial phenotype in the control and E. coli infected groups (P>0.05). Real-time RT-PCR and Western blot analysis showed that there was a significant increase of BDNF, TrkB, p-Akt and Survivin mRNA and protein expression during postnatal 7 days in the E. coli infected group (P<0.05). Our results suggest that endogenous neurogenesis may occur in hippocampus in early postnatal period and may be enhanced by neonatal inflammation reactive syndrome. The PI3K/Akt signaling pathway may be involved in regulation of BDNF and is important in the potential activation of neuroprotective and repair pathways during critical time windows of hippocampal development.
Brain research 04/2012; 1459:1-14. · 2.46 Impact Factor
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ABSTRACT: In this paper, we present a novel method to detect violent scenes in movies. The detection process involves two views-audio and video views. For the audio view, a supervised method based on HCRFs is exploited to improve the classification performance. For the video view, we detect violent shots by locating the violent areas. Finally, the auditory and visual classifiers are combined in a co-training way. The experimental results on several movies with violent contents preliminarily show the effectiveness of our method.
Computational Intelligence and Security (CIS), 2010 International Conference on; 01/2011
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ABSTRACT: To construct and identify 2 secreted human GAD65 fragment DNA vaccines.
The GAD(190-315), GAD(490-570) cDNA and hIL-2 signal peptide cDNA were linked through overlapping PCR, respectively. The fusion gene was cloned into eukaryotic expression vector pBudCE4.1. After the DNA vaccine being determined to contain the correct target nucleotide sequence, the expression of fusion proteins was detected by Western blot.
The nucleotide sequence of the cloned gene was the same as the reported sequence, and their open reading fragment was correct. The products of these DNA vaccines were expressed and secreted in eukaryotic cell using Western blot.
The pBudCE4.1/SGAD(190-315) and pBudCE4.1/SGAD(490-570) secreted human GAD65 fragment DNA vaccines were successfully constructed, which is a foundation for immune prevention of type 1 diabetes.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 01/2008; 32(6):997-1001.
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ABSTRACT: To demonstrate the existence of islet-associated antigen-specific T cells in some of type 1B patients.
Peripheral blood samples were obtained from 23 classical type 1 diabetes (Type 1A) patients, 29 type 1B patients, and 16 healthy control subjects. Islet-associated autoantibodies were determined by radioligand assay. Human peripheral blood mononuclear cells were isolated by Ficoll-Hypaque density gradients and enzyme linked immune spot (ELISPOT) assay was performed to measure the number of spots-forming plaque. One spot-forming represents a GAD65-reactive IFN-gamma-secreting T (IFN-gamma-T) cell.
The IFN-gamma-spot numbers stimulated with GAD65 were 12 (10.3-20.9) (median and 95% CI) in Type 1A group, 3.5 (3.0-5.7) in Type 1B group, and 1.0 (0.3-1.8) in Control group respectively. The frequency of spots significantly increased in Type 1B group compared to Control group (P<0.05) and there were significantly more spot numbers in Type 1A group than in Type 1B or Control group (both P<0.01). A positive response was determined when the spot number>95% CI of Control subjects. 48.3% (14 of 29) in Type 1B group, 78.3% (18 of 23) in Type 1A and no subject (0) in Control group had positive response, while the numbers of IFN-gamma-spot reactive to phytohemagglutinin (PHA) did not differ among the three groups.
GAD65-reactive IFN-gamma-T cells are found in similar to Type 1A patients, that abnormal T cell-mediated immunity involved in the underlying aetiology and pathogenesis is also present in some Chinese patients initially diagnosed as with Type 1B diabetes. More rigorous screening for these conditions is needed before classifying subjects as having Type 1B diabetes and ELISPOT assay detecting IFN-gamma-T cells reactivity against GAD65 may contribute to the diagnosis of "autoimmune diabetes mellitus".
Zhonghua yi xue za zhi 04/2007; 87(16):1102-5.
