Wei Liu

Sichuan University, Hua-yang, Sichuan, China

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Publications (307)525.45 Total impact

  • Molecular and Clinical Oncology 04/2015; DOI:10.3892/mco.2015.552
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    ABSTRACT: Surgery is currently the preferred treatment choice for mid-esophageal diverticula, while endoscopic therapy is rapidly establishing itself. We report the first two cases of giant mid-esophageal diverticula presented with dysphagia successfully treated with per-oral endoscopic myotomy (POEM). There were no complications during the procedure and the patients' conditions improved remarkably within short time of recovery. POEM could provide a safe, effective and less invasive treatment of mid-esophageal diverticula if appropriately used. Further studies on long-term efficacy with larger number of cases are necessary.
    Surgical Endoscopy 04/2015; DOI:10.1007/s00464-015-4181-2 · 3.31 Impact Factor
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    ABSTRACT: Myeloperoxidase is a key component of neutrophil granules involved in killing engulfed microorganisms. We obtained a zebrafish mutant (smu681) lacking Sudan black staining by large-scale screening, which was a neutrophil-replete but myeloperoxidase-deficient mutant. When infiltrated with Candida albicans, smu681 embryos and sibling embryos showed similar survival after infection. Proliferation of C. albicans was more rapid in smu681 embryos than in sibling embryos, although it was eventually suppressed. In addition, the number of neutrophils accumulating at the site of infection was significantly larger in mutant embryos than in sibling embryos, and mutant embryos showed increased expression of several inflammatory cytokines after C. albicans infection. These findings indicate that myeloperoxidase deficiency alters the inflammatory response to fungal infection. Copyright © 2015. Published by Elsevier Ltd.
    Fish &amp Shellfish Immunology 02/2015; 44(1). DOI:10.1016/j.fsi.2015.01.038 · 3.03 Impact Factor
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    ABSTRACT: Abstract Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. Previous studies demonstrated that engineered Escherichia coli strains can produce P3HP with supplementation of expensive vitamin B12. The present study examined the production of P3HP from glycerol in the recombinant Klebsiella pneumoniae strain, which naturally synthesizes vitamin B12. The genes glycerol dehydratase and its reactivation factor (dhaB123, gdrA and gdrB from K. pneumoniae), aldehyde dehydrogenase (aldH from E. coli) were cloned and expressed in K. pneumoniae to produce 3-hydroxypropionate (3HP), with two genes (dhaT and yqhD) for biosynthesis of 1,3-propanediol were deleted. To obtain P3HP production, propionyl-CoA synthetase (prpE from E. coli) and polyhydroxyalkanoate synthase (phaC from Ralstonia eutropha) were introduced. Under the appropriate aeration condition, the cell yield and P3HP content were 0.24 g/L and 12.7% (wt/wt [cell dry weight]) respectively along with 2.03 g/L 3HP after 48 h cultivation. Although the yield is relatively low, this study shows the feasibility of producing P3HP in K. pneumoniae from glycerol without vitamin B12 for the first time. The results also suggest that the aeration conditions should be optimized carefully for the efficient production of P3HP.
  • Endoscopy 01/2015; 47 Suppl 1(S 01):E30-1. DOI:10.1055/s-0034-1391133 · 5.20 Impact Factor
  • Endoscopy 01/2015; 47(S 01):E85-E86. DOI:10.1055/s-0034-1391236 · 5.20 Impact Factor
  • Chinese Journal 01/2015; 60(7):645. DOI:10.1360/N972014-01183
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    ABSTRACT: As a typical biomineral, hydroxyapatite (HAp) is widely applied in bone implants and other related fields. However, the inherent nature of HAp can potentially be altered through restricting its fabrication conditions. Here, HAp fabricated by a hydrothermal treatment of pieces of natural seashell is demonstrated to have the capability of fluorescence quenching. To the best of the author's knowledge, this is the first time that this new property of HAp has been reported. Consequently, we assembled a fluorescence-quenching platform based on the biomineralized HAp substrate following a hydrothermal treatment and associated with a DNA molecular beacon and applied to cancer cell detection by the transformation from "OFF state" (fluorescence quenching) to "ON state" (fluorescence recovery). Herein, we found that the outer surface of HAp material after hydrothermal biomineralization for 5 days has considerable capability for both fluorescence quenching and recovery. These results may also have implications in the further detection of various targets such as cancer cells with other special surface antigens, significant biological small molecules or disease related microRNA, just by changing the sequence of the nucleic acid beacon according to the corresponding aptamer.
    Scientific Reports 12/2014; 4:7556. DOI:10.1038/srep07556 · 5.08 Impact Factor
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    ABSTRACT: Short chain diols (propanediols, butanediols, pentanediols) have been widely used in bulk and fine chemical industries as fuels, solvents, polymer monomers and pharmaceutical precursors. The chemical production of short chain diols from fossil resources has been developed and optimized for decades. Consideration of the exhausting fossil resources and the increasing environment issues, the bio-based process to produce short chain diols is attracting interests. Currently, a variety of biotechnologies have been developed for the microbial production of the short chain diols from renewable feed-stocks. In order to efficiently produce bio-diols, the techniques like metabolically engineering the production strains, optimization of the fermentation processes, and integration of a reasonable downstream recovery processes have been thoroughly investigated. In this review, we summarized the recent development in the whole process of bio-diols production including substrate, microorganism, metabolic pathway, fermentation process and downstream process.
    Microbial Cell Factories 12/2014; 13(1):165. DOI:10.1186/PREACCEPT-5297584212857603 · 4.25 Impact Factor
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    ABSTRACT: Multi-dimensional hydroxyapatite (HAp) nanoclusters are fabricated for the first time via a membrane assisted fabrication utilising a Nafion N-117 membrane. The effect of biomineralization time upon the resulting morphology is investigated in detail with the likely crystal growth mechanism proposed. This work opens up the new possibility for preparing HAp with other specific morphological alternatives and is of great value for applications in a plethora of fields.
    New Journal of Chemistry 11/2014; 39(1). DOI:10.1039/C4NJ01292E · 3.16 Impact Factor
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    ABSTRACT: Using the first principle calculations, the widths of piezoelectric charge distributions in an Ag-ZnO-Ag transistor are obtained, which are key parameters for understanding the piezotronic effect.•The modulations of Schottky barriers at two transistor interfaces show asymmetric behavior due to piezotronic effect, which agrees with previous experiment results.
    Nano Energy 10/2014; DOI:10.1016/j.nanoen.2014.10.014 · 10.21 Impact Factor
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    ABSTRACT: Urinary KIM-1 is an ideal biomarker for acute kidney injury diagnosis. The proof-of-concept is demonstrated by utilizing the hydroxyapatite derived from natural fish scales as an electrode material, where the sensing of KIM-1 is shown to be possible for the first time with a linear range from 0.01 to 0.20 μg mL(-1) and a detection limit of 0.017 μg mL(-1) under model conditions; proof-of-concept is demonstrated in spiked urine.
    The Analyst 09/2014; 139(21). DOI:10.1039/c4an00957f · 3.91 Impact Factor
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    ABSTRACT: The correlation between rheological behavior and time evolution of the phase separation patterns was investigated in the epoxy/thermoplastic blends. Before and during the induction period of phase separation, the storage and loss modulus initially increased with epoxy curing reaction and the concentration fluctuation. At the late stage of phase separation, the modulus values also increased and showed a sharp enhancement around the epoxy gel point. However, the time evolution during the reaction induced phase separation process differed a lot at various thermoplastic (TP) concentrations. At the low TP concentrations, the rheological parameters decreased with the coarsening of sea-island structure. At the high TP concentrations, the TP-rich continuous structures initially formed and maintained until the end, resulting in a continuous increase for the rheological characters. At middle TP concentrations, formation and evolution of the three-layered structure displayed a complicated rheological behavior. It was found that the storage modulus quickly increased, reached a vertex, then rapidly decreased, reached a minimum, and increased again afterwards. Although the rheological behaviors were almost phenomenologically similar as that in the normal dynamically symmetric system, driving force for the variation was fundamentally different. Especially for the case of middle TP concentrations, the behavior of the holistic volume shrinking of the slow dynamic TP-rich network and the flowing out of the fast dynamic epoxy-rich phase from the network during this period, as radically transformed the nature of the matrix from an elastic network to a macro-phase separated layer structure and caused the dramatic change of the rheological behaviors.
    Polymer 09/2014; 55(19). DOI:10.1016/j.polymer.2014.08.007 · 3.77 Impact Factor
  • Digestive Diseases and Sciences 06/2014; 59(12). DOI:10.1007/s10620-014-3232-1 · 2.55 Impact Factor
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    ABSTRACT: The morphology evolution and the corresponding linear viscoelastic behavior of the phase-separating polybutadiene (PB)/low vinyl content polyisoprene (LPI) blend have been investigated by phase contrast optical microscopy (PCOM), small-angle light scattering (SALS) and rheometry. Two kinds of structure evolutions and rheological responses have been observed. It is found that the co-continuous structure generally gives a power law behavior of the dynamic storage modulus versus frequency and the coarsening of co-continuous structure leads to a decrease of the storage modulus. For the droplet-matrix structure, a platform modulus is observed at the mediate frequencies, followed by the typical terminal relaxation behavior of storage modulus at the extremely low frequencies. The decreasing platform modulus and increasing terminal modulus with the growth of droplets are observed and can be well interpreted by the simplified Palierne model. The platform modulus and terminal modulus at a given frequency are found to be scalable with the phase separation time. Besides, the characteristic relaxation time and domain size of the droplets have been obtained by rheology. And it seems that the rheologically determined droplet dimensions are consistent with the ones determined by PCOM and SALS.
    Chinese Journal of Polymer Science 06/2014; 32(6):718-730. DOI:10.1007/s10118-014-1443-8 · 1.42 Impact Factor
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    ABSTRACT: To evaluate the safety and efficacy of endoscopic retrograde cholangiopancreatography (ERCP) in children with pancreaticobiliary diseases and the characteristics of pancreaticobiliary disorders in children.
    Zhonghua er ke za zhi. Chinese journal of pediatrics 05/2014; 52(5):328-32.
  • Polymer 05/2014; 55(11). DOI:10.1016/j.polymer.2014.04.026 · 3.77 Impact Factor
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    ABSTRACT: Monounsaturated fatty acids (MUFAs) are the best components for biodiesel when considering the low temperature fluidity and oxidative stability. However, biodiesel derived from vegetable oils or microbial lipids always consists of significant amounts of polyunsaturated and saturated fatty acids (SFAs) alkyl esters, which hampers its practical applications. Therefore, the fatty acid composition should be modified to increase MUFA contents as well as enhancing oil and lipid production. The model microorganism Escherichia coli was engineered to produce free MUFAs. The fatty acyl-ACP thioesterase (AtFatA) and fatty acid desaturase (SSI2) from Arabidopsis thaliana were heterologously expressed in E. coli BL21 star(DE3) to specifically release free unsaturated fatty acids (UFAs) and convert SFAs to UFAs. In addition, the endogenous fadD gene (encoding acyl-CoA synthetase) was disrupted to block fatty acid catabolism while the native acetyl-CoA carboxylase (ACCase) was overexpressed to increase the malonyl coenzyme A (malonyl-CoA) pool and boost fatty acid biosynthesis. The finally engineered strain BL21DeltafadD/pE-AtFatAssi2&pA-acc produced 82.6 mg/L free fatty acids (FFAs) under shake-flask conditions and FFAs yield on glucose reached about 3.3% of the theoretical yield. Two types of MUFAs, palmitoleate (16:1Delta9) and cis-vaccenate (18:1Delta11) made up more than 75% of the FFA profiles. Fed-batch fermentation of this strain further enhanced FFAs production to a titer of 1.27 g/L without affecting fatty acid compositions. This study demonstrated the possibility to regulate fatty acid composition by using metabolic engineering approaches. FFAs produced by the recombinant E. coli strain consisted of high-level MUFAs and biodiesel manufactured from these fatty acids would be more suitable for current diesel engines.
    Biotechnology for Biofuels 04/2014; 7(1):59. DOI:10.1186/1754-6834-7-59 · 6.22 Impact Factor
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    ABSTRACT: Coarsening is a general phenomenon in phase separating mixtures. In this study, we report a hierarchical coarsening at different length scales. Dispersed domains grew by direct combination of two/three small ones while some small domains can survive for a long time. The small angle laser light scattering showed that the scattered intensity exhibited multiple "decay-growth" transitions in the coarsening process. The evolution of the main peak gave a characteristic power law index of about -3/4, which is much faster than the -1/3 relationship from the traditional theories. We propose that relaxation and hydrodynamic flow play important roles in this process.
    Soft Matter 03/2014; 10(15):2649-55. DOI:10.1039/c3sm52713a · 4.15 Impact Factor
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    ABSTRACT: We investigated the interplay between viscoe-lastic phase separation (VPS) and crystallization in a dynamically asymmetric crystalline/crystalline polymer blend system of poly(butylene succinate) (PBS)/poly(ethylene oxide) (PEO). The dynamic asymmetry came from the large molecular weight difference between the slow relaxing PEO and the fast relaxing PBS molecules. The evolution of network-like structures, phase inversion, and volume shrinking processes were studied by a phase contrast microscope (PCOM). The crystallization kinetics of PBS was determined by a differential scanning calorimeter (DSC). It was found that both the crystal nucleation and growth of PBS were strongly influenced by the existence and relaxation of the entangled PEO network, even though PEO was the minor component. The nuclei of PBS were more inclined to form at the interface between phase domains due to the effect of interface-assisted crystallization. Thus, the nucleation density decreased with the decrease of interfacial area. Meanwhile, the corresponding crystal growth rate was also dependent on the VPS processes. After a second quench to a temperature below the melting point of PEO, the spatial distribution of both components and the final crystallization morphology were also significantly controlled by the initial phase structures. Compared to dynamically symmetric polymer systems, the VPS process provided more versatile pathways to control the phase and crystallization morphologies in such immiscible crystalline/crystalline polymer blends.
    Macromolecules 02/2014; 47(5):1741-1748. DOI:10.1021/ma5001496 · 5.93 Impact Factor

Publication Stats

3k Citations
525.45 Total Impact Points


  • 2011–2015
    • Sichuan University
      • • Department of Gastroenterology and Hepatology
      • • Department of Orthopedic Surgery
      Hua-yang, Sichuan, China
    • Dalian University of Technology
      • School of Software
      Lü-ta-shih, Liaoning, China
    • Jiangsu Academy of Agricultural Sciences
      Nan-ching-hsü, Jiangxi Sheng, China
    • Hospital of the University of Pennsylvania
      • Department of Microbiology
      Filadelfia, Pennsylvania, United States
  • 2010–2015
    • Southern Medical University
      • Department of Cell Biology
      Shengcheng, Guangdong, China
    • University of California, Berkeley
      • Department of Mechanical Engineering
      Berkeley, California, United States
    • Nankai University
      • TEDA School of Biological Science and Biotechnology
      T’ien-ching-shih, Tianjin Shi, China
  • 2001–2015
    • Chinese Academy of Sciences
      • • Institute of Chemistry
      • • Qingdao Institute of Bioenergy and Bioprocess Technology
      • • State Key Laboratory of Environmental Chemistry and Ecotoxicology
      • • South China Botanical Garden
      • • Institute of Metal Research
      • • Institute of Biophysics
      Peping, Beijing, China
  • 2014
    • Technical Institute of Physics and Chemistry
      Peping, Beijing, China
  • 2002–2014
    • Huazhong University of Science and Technology
      • • National Engineering Research Center for Nanomedicine
      • • Department of Infectious Diseases
      • • Department of Electronics and Information Engineering
      Wu-han-shih, Hubei, China
  • 2012–2013
    • Huazhong Agricultural University
      • Division of Animal Infectious Disease
      Wu-han-shih, Hubei, China
  • 2011–2013
    • Jilin University
      • State Key Lab of Theoretical and Computational Chemistry
      Yung-chi, Jilin Sheng, China
  • 2010–2013
    • Nanyang Technological University
      • • School of Electrical and Electronic Engineering
      • • Luminous Center of Excellence for Semiconductor Lighting and Displays
      • • Centre for Multimedia and Network Technology (CeMNet)
      Tumasik, Singapore
  • 2007–2012
    • JiangHan University
      Jiang’an, Hubei, China
    • Tianjin University of Traditional Chinese Medicine
      T’ien-ching-shih, Tianjin Shi, China
  • 2010–2011
    • University of Shanghai for Science and Technology
      Shanghai, Shanghai Shi, China
  • 1991–2010
    • Albert Einstein College of Medicine
      • Department of Otorhinolaryngology - Head & Neck Surgery
      New York City, New York, United States
  • 2009
    • Nanjing University of Aeronautics & Astronautics
      Nan-ching, Jiangsu Sheng, China
    • Huazhong (Central China) Normal University
      Wu-han-shih, Hubei, China
  • 2008–2009
    • Hohai University
      Nan-ching, Jiangsu Sheng, China
    • Hubei University
      Wu-han-shih, Hubei, China
  • 2007–2008
    • Shandong University
      • School of Pharmaceutical Sciences
      Chi-nan-shih, Shandong Sheng, China
  • 2005
    • Hunan University
      • College of Chemistry and Chemical Engineering
      Ch’ang-sha-shih, Hunan, China
  • 1991–2001
    • Academia Sinica
      • Institute of Physics
      T’ai-pei, Taipei, Taiwan
  • 1998
    • Harvard University
      Cambridge, Massachusetts, United States