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ABSTRACT: Aims: To investigate the variations of OX40 (tumor necrosis factor receptor superfamily, member 4) and its ligand OX40L genes and their relationships with serum lipids and apolipoproteins (apo) levels in Chinese healthy individuals and patients with endogenous hypertriglyceridemia (HTG) in the Chengdu area. Methods: The genotypes and allele frequencies of the rs3850641 and rs17568 polymorphisms in the OX40L and OX40 genes were assayed by polymerase chain reaction and restriction fragment length polymorphism. Results: In the case-control study, which included 126 HTG subjects and 206 normal control subjects, the frequencies of the G allele at the rs3850641 site and the G allele at the rs17568 site in the patients were similar to those observed in the controls. In the HTG group, subjects with G allele carriers of the rs3850641 site had lower serum high-density lipoprotein cholesterol and apo AI levels as compared to those of genotype AA. In the case group, subjects with G allele carriers of the rs17568 site had higher serum low-density lipoprotein cholesterol (LDL-C) levels, while controls had lower serum total serum cholesterol and LDL-C levels. Conclusion: These results suggest that the rs3850641 and rs17568 polymorphisms in the OX40L and OX40 genes are associated with some of the lipid and lipoprotein variations in subjects with endogenous HTG and/or in the general population of Han Chinese.
Genetic Testing and Molecular Biomarkers 12/2012; · 1.11 Impact Factor
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ABSTRACT: INTRODUCTION: Recent studies have shown that interleukin (IL)-16 is an immunomodulatory cytokine, which plays an important role in some inflammatory and autoimmune diseases. We aimed to investigate the association between the IL-16 gene polymorphisms and presence of coronary artery disease (CAD) where inflammatory processes are involved. METHODS: This case-control study enrolled 651 CAD patients confirmed by coronary angiography and 428 controls. Four tag single nucleotide polymorphisms (rs8034928-rs3848180-rs4577037-rs1131445) within the IL-16 gene and the related haplotypes were genotyped by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Plasma IL-16 concentration was measured by enzyme-linked immunosorbent assay. RESULTS: In patients with CAD, the plasma concentration of IL-16 was significantly higher than in controls (97.6 ± 10.7, 66.5 ± 9.6, respectively. p < 0.001). By using multivariate logistic regression analysis, the allele and genotype frequencies of rs8034928 were different between CAD and control groups (P < 0.001). However, the associations of the polymorphisms rs3848180,rs4577037,and rs1131445 with CAD were not observed. The haplotypes TTTT and TGGT significantly increased risk to CAD (OR, 95% CI: 1.43, 1.26-1.63; 1.47, 1.16-1.85; respectively), whereas the haplotypes CTTT and TTGT referred to protection of CAD (OR, 95% CI: 0.45, 0.33-0.62; 0.50, 0.33-0.76; respectively). CONCLUSION: The study indicated that the IL-16 rs8034928 T/C polymorphism and haplotypes were associated with the presence of CAD in Chinese Han population. The IL-16 gene polymorphisms may be a useful predictor to the susceptibility of CAD.
Clinical biochemistry 11/2012; · 2.02 Impact Factor
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ABSTRACT: To investigate whether high-fat diet induced obesity was associated with variation of glucose absorption in small intestinal mucosa of rats.
46 male SD rats were randomly divided into high-fat diet group (n = 31) and control group (n = 15), fed with high-fat diet and normal diet for 24 weeks, respectively. After 24 weeks, the rats were divided into obese (n = 16) and obesity-resistant group (n = 10) according to their body weight. Rats' body weight, abdominal fat weight, plasma glucose level, maltase, sucrase activity in small intestinal mucosa were measured. SGLT-1 expression in intestinal mucosa was detected by immunohistochemistry, RT-PCR and Western blot.
Mean body weight, abdominal fat weight, fast plasma glucose levels, maltase activities and SGLT-1 protein expression in intestinal mucosa of obese rats were significantly higher than those in the control and obesity-resistant rats (P < 0.05). Sucrase activities in intestinal mucosa showed no statistical difference among the three groups (P > 0.05). The SGLT-1 mRNA expression in obese group was increased by 12.5% and 23% when compare with the control and obesity-resistant group, respectively. But the difference was not statistical significant (P > 0.05).
High-fat diet induced obesity was associated with the increased intestinal maltase activity and expression of SGLT-1 in rats, the key molecule in glucose absorption.
Wei sheng yan jiu = Journal of hygiene research 11/2012; 41(6):878-82.
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ABSTRACT: A series of novel indolylquinones have been synthesized by treating halogeno-quinone with 2-substituated indole derivatives in the presence of kalium carbonate and TEBA in acetonitrile at room temperature. These compounds were evaluated for their antiproliferative activity against human MDA-MB-231 and MCF-7 breast cancer cell lines. All the tested compounds showed potent mircomolar cytotoxicity activity in both breast cancer cell lines. 3d (IC(50) value=2.29 μg/mL for MCF-7 cells) and 3g (IC(50) value=3.99 μg/mL for MDA-MB-231 cells) displayed the most potent antiproliferative activity of the series. Also, in vitro anticancer activity of the compounds further showed that bis-indolylquinones were more active than mono-indolylquinones. Fluorescence microscopy analysis indicated that compound 3d and 3g inhibited breast cancer cells proliferation by triggering apoptotic cell death.
European journal of medicinal chemistry 05/2012; 54:42-8. · 3.27 Impact Factor
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ABSTRACT: To investigate the effects of the somatostatin analogue, octreotide, on maltose and sucrase activities and expression of glucose transporter type 2 (GLUT2) in obese rat intestinal mucosa.
We divided 49 Sprague-Dawley rats into a group of 31 high fat diet-induced obese rats and a group of 18 normal controls. The obese rats were separated into an octreotide treated group of 16 rats and an obese group of 15. The intervention group was injected with octreotide at 40 μg/kg body weight every 12 h for 8 d. Rat body weight was measured weekly to calculate Lee's index. After euthanization, maltase and sucrase activities in the small intestine were measured by activity assays, and the fasting plasma glucose level was measured. The expression of GLUT2 in small intestinal mucosa was analyzed by immunohistochemistry, reverse transcriptase polymerase chain reaction and Western blotting assays.
Body weight, Lee's index, fasting plasma glucose level, maltase activity in small intestinal mucosa, mucosa and apical GLUT2, GLUT2 mRNA and protein expression levels were all significantly higher in the obese group than in the normal control group (605.61 ± 141.00 vs 378.54 ± 111.75, 337.61 ± 10.82 vs 318.73 ± 20.10, 8.60 ± 1.38 vs 7.33 ± 0.70, 156.01 ± 58.81 vs 50.43 ± 30.49, 390 744.2 ± 62 469.21 vs 170 546.50 ± 50 646.14, 26 740.18 ± 3809.60 vs 354.98 ± 57.19, 0.26 ± 0.11 vs 0.07 ± 0.02, and 2.08 ± 0.59 vs 1.27 ± 0.38, respectively, all P < 0.01). Sucrase activity did not differ between the two groups. Octreotide intervention significantly decreased the body weight and fasting plasma glucose level of obese rats (508.27 ± 94.39 vs 605.61 ± 141.00, 7.58 ± 1.51 vs 8.60 ±1.38, respectively, all P < 0.05). The intestinal mucosa and apical GLUT2, expression of GLUT2 mRNA and protein were also significantly lower in the octreotide intervention group than in the obese group (269 975.2 ± 53 730.94 vs 390 744.2 ± 62 469.21, 3758.06 ±364.51 vs 26 740.18 ± 3809.60, 0.08 ± 0.02 vs 0.26 ±0.11, and 1.31 ± 0.27 vs 2.08 ± 0.59, respectively, all P < 0.01).
High fat diet-induced obesity is associated with elevated intestinal maltase activity, GLUT2 expression, and permanent apical GLUT2 in the small intestinal mucosa of rats. Octreotide can inhibit these effects.
World Journal of Gastroenterology 10/2011; 17(39):4434-9. · 2.47 Impact Factor
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ABSTRACT: To determine whether the single nucleotide polymorphism (SNP) on chromosome 12q24.31(rs2259816) is associated with coronary artery disease (CAD) in Han population of southwest China.
A case-control association study with 592 unrelated patients with coronary artery disease and 463 normal controls from Chinese Han population was performed. Genotype for the SNP on chromosome 12q24.31 (rs2259816) was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The genotypes of AA, AC, CC were both detected in the coronary artery disease group and the control group. The frequencies of A allele were 49.5% in case group and 43.8% in control group, showing statistically significant difference(OR=1.129, 95%CI:1.029-1.239, P=0.010).
The replication study showed that the genetic polymorphism in rs2259816 is associated with coronary artery disease in Han population of southwest China.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 08/2011; 28(4):455-9.
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ABSTRACT: Coronary artery disease (CAD) is multifactorial disease which occurs as a result of the interaction of genetic and environmental factors. Obesity is an independent risk factor for cardiovascular disease. Recent genome-wide association studies have identified several genes associated with obesity in Europeans. We wondered whether these genetic variants were associated with CAD. Three single nucleotide polymorphisms (SNPs) rs7561317 near TMEM18, rs7138803 near BCDIN3D/FAIM2 and rs12970134 near MC4R were examined in 930 Han Chinese subjects based on coronary angiography, using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis. There were no significant differences in genotypes and allele distributions of three SNPs between CAD and CAD-free groups. The AA genotype of SNP rs12970134 near MC4R was associated to obesity both in CAD group and CAD-free group in Han Chinese population (P < 0.001, OR = 2.96, 95% CI 2.01-3.73; and P = 0.003, OR = 2.59, 95% CI 1.86-3.19, respectively). Our observations suggest that the polymorphism rs12970134 near MC4R may be associated to the risk of obesity in Han Chinese population.
Molecular Biology Reports 05/2011; 39(2):1739-44. · 2.93 Impact Factor
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ABSTRACT: OX40 and OX40L, members of the tumor necrosis factor receptor superfamily, are costimulatory molecules involved in the activation and proliferation of T lymphocytes. OX40L plays an important role in the process of atherosclerosis, and variants of OX40/OX40L are associated with myocardial infarction in European populations. Our study examined 235 patients with acute coronary syndrome (ACS) and 220 controls and sought to establish whether polymorphisms in OX40/OX40L are associated with atherosclerosis or myocardial infarction in the Han Chinese population. OX40 rs17568A/G, rs2298212A/G, and OX40L rs3850641A/G polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method. The results showed that carriers of the G allele of rs17568A/G had a significantly increased risk of ACS (p = 0.023, adjusted odds ratio = 1.72, 95% confidence interval = 1.08-2.75) after adjusting for age, sex, diabetes, hypertension, and lipids. No significant association between rs2298212A/G or rs3850641A/G and the risk of ACS was found in this study. In conclusion, OX40 gene polymorphism may be associated with a risk of ACS in the Han Chinese population, although the association between OX40L polymorphisms and ACS requires further investigation.
DNA and cell biology 04/2011; · 2.28 Impact Factor
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ABSTRACT: To clarify whether a VE-cadherin-dependent pathway allows mini-TrpRS to inhibit mini-TrpRS-induced new blood vessel formation in endothelial cells (ECs), the inhibitory effects of mutant mini-TrpRS and VE-cadherin on mini-TrpRS-induced angiogenesis were investigated. The effects of mini-TyrRS and mini-TrpRS on EC proliferation were evaluated using an MTT colorimetric assay. Cell migration was assayed using a modified Boyden chamber technique. The angiogenic activity in vitro was evaluated by transwell migration assay and matrigel-induced capillary tube formation. It was found that mini-TrpRS does not inhibit the mini-TyrRS-induced proliferation and migration of EC under the condition of VE-cadherin knockout. While wild-type mini-TrpRS inhibited mini-TyrRS-induced angiogenesis, this activity vanished for the mutant protein. Also, the promotion of angiogenesis by mini-TyrRS and the inhibition of angiogenesis by mini-TrpRS were VEGFR2 dependent but not VEGF dependent. Mini-TyrRS was able to increase the protein expression of VEGFR-2 in the presence of VE-cadherin, while no stimulatory effect of mini-TyrRS was detected when VE-cadherin was not present. Angiogenesis is therefore stimulated by mini-TyrRS and inhibited by mini-TrpRS, raising the possibility that mini-TyrRS and mini-TrpRS stimulate a common downstream signaling event: VE-cadherin. Thus, naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activities on angiogenesis. The opposing activities of the two tRNA synthetases suggest tight regulation of the balance between pro- and antiangiogenic stimuli.
Heart and Vessels 03/2011; 27(2):193-201. · 2.05 Impact Factor
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ABSTRACT: To investigate the association between coronary heart diseases and single nucleotide polymorphism (SNP) in rs174570 in Han Chinese Populations.
A case-control study was undertaken in Han Chinese in Sichuan province, with 264 patients with coronary heart diseases and 303 normal controls. The genotypes of SNP in rs174570 were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The genotypes of CC/CT/TT were detected in both case and control groups. No significant differences in the frequencies of the three genotypes were found between the two groups. The qualitative and quantitative analyses of the coronary angiography showed no significant differences in the patients with coronary heart diseases with the three genotypes. In the normal controls, high frequencies of C allele were associated with higher levels of low density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) (P < 0.05).
Genetic polymorphism in rs174570 is not associated with the development and severity of coronary heart diseases despite its influences on serum LDL-C levels in Han Chinese Populations.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 03/2011; 42(2):245-8.
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ABSTRACT: The purpose of this study was to determine the mechanism of mini-tyrosyl-tRNA synthetase/mini-tryptophanyl-tRNA synthetase (mini-TyrRS/mini-TrpRS) on ischemic angiogenesis in rats with acute myocardial infarction and proliferation, migration, potential signaling pathways of rat coronary venular endothelial cells (RCVECs). The effects of mini-TyrRS/mini-TrpRS on RCVECs proliferation were evaluated using the MTT colorimetric assay. Cell migration was assayed using a modified Boyden chamber technique. The potential involvement of Erk and PI3K signaling pathways was explored using selective chemical inhibitor or Western-blot analysis. Left coronary artery ligation was used to establish the model of acute myocardial infarction in rats (Sprague-Dawley male rats, 200-250 g, 2-3 months old), 20 μl of mini-TyrRS, mini-TrpRS, or PBS (vehicle) was injected subcutaneously every 12 h. The rats were randomly divided into four experimental groups: sham operated group; coronary artery ligation (CAL); CAL + mini-TyrRS (20 μl, twice daily, 600 μg kg(-1) day(-1)); and CAL + mini-TrpRS (20 μl, twice daily, 600 μg kg(-1) day(-1)). The experiment was carried out at four time points on the 3rd, 7th, 14th, and 28th day after ligation. To determine whether mini-TyrRS/mini-TrpRS affected the angiogenesis activity of rats with myocardial infarction, we measured the myocardial infarction size by TTC staining, and microvessel density (MVD) was determined by CD34 staining. The results show that proliferation and migration in RCVECs could be promoted by mini-TyrRS at concentrations of 1-100 μg/ml, and inhibited by mini-TrpRS. Phospho-PI3-kinase and Erk expression increased significantly when mini-TyrRS was added, but could be attenuated by mini-TrpRS. Compared to the CAL group, the myocardial infarction size of the mini-TyrRS group at the 3rd, 7th, 14th, and 28th day were decreased, while mini-TrpRS increased, but only in days 14 and 28 was there a significant difference. Except that, the microvessel density of RCVECs was promoted in mini-TyrRS group but inhibited in the mini-TrpRS group. These results indicated that angiogenesis could be either stimulated by mini-TyrRS or inhibited by mini-TrpRS.
Heart and Vessels 10/2010; 26(1):69-80. · 2.05 Impact Factor
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ABSTRACT: We aimed to clarify the different angiogenesis effects of mini-tyrosyl-tRNA synthetase (TyrRS)/minitryptophanyl-tRNA synthetase (TrpRS) in rodent primates with acute myocardial infarction, by delivering small interfering RNAs (siRNAs) systemically in a liposomal formulation. Left coronary artery ligation was used to establish the model of acute myocardial infarction in rats; mini-TyrRS/mini-TrpRS-specific siRNAs were encapsulated in stable nucleic acid lipid particles (SNALP), and administered by intravenous injection to rats. Rats were divided into four experiment groups: sham operated group (no left anterior descending artery [LAD] occlusion); negative control group (LAD occlusion + saline injection); mock transfection group (LAD occlusion + mock transfected injection); experiment group (LAD occlusion + mini-TyrRS/mini-TrpRS-specific siRNAs injection). Silencing efficiency was assayed by Western blotting. To determine whether mini-TyrRS/mini-TrpRS affected the angiogenesis activity of rats with myocardial infarction, we measured the myocardial infarction size by TTC staining, and the capillary density using immunohistochemistry staining, to investigate the expression of factor VIII. The myocardial infarction size and the capillary density of mini-TyrRS-siRNA group were respectively 18.89% and 8.64/0.1 mm(2) 1 month after ligation, while in the mini-TrpRS-siRNA group these values were 7.33% and 17.32/0.1 mm(2), significantly different compared with the mock transfection group (14.19%; 13.56/0.1 mm(2)) and negative control group (14.28%; 13.89/0.1 mm(2)), P < 0.05. There were no significant changes between the mock transfection group and the negative control group, P > 0.05. These results indicated that angiogenesis is either stimulated by mini-TyrRS or inhibited by mini-TrpRS in rat models with acute myocardial infarction.
Heart and Vessels 07/2010; 25(4):324-32. · 2.05 Impact Factor
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ABSTRACT: Objectives: Previously, a genome-wide scan has identified a nonsynonymous single nucleotide polymorphism (rs3812316, G771C, Gln241His) in the MLXIPL gene that is associated with the level of plasma triglycerides. However, no data are available on the association of this polymorphism with coronary artery disease (CAD) in the Chinese population. The aim of this study was to evaluate the association between a gene polymorphism related to triglyceride metabolism and CAD. Methods: The genotype of the polymorphism in the MLXIPL gene was determined in 352 CAD patients and 152 CAD-free subjects. All of the participants were selected to study the MLXIPL gene rs3812316 polymorphism using the polymerase chain reaction restriction fragment length polymorphism method. Results: In Chinese participants, we observed that there was a significant difference in genotype between the cases and controls (p = 0.002). After allowance for potential confounders, unconditional logistic analysis revealed that the SNP was significantly related to a risk in CAD patients (adjusted OR 2.96, 95% CI 1.30-5.08; p =0.004). We also found that there was a significant association between the single nucleotide polymorphism and plasma triglyceride levels (OR 1.28, 95% CI 1.061-1.542; p < 0.05). Conclusion: The gene sequence variation in the MLXIPL gene may serve as a novel genetic marker for the risk of significant CAD.
Cardiology 08/2009; 114(3):174-8. · 1.71 Impact Factor
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ABSTRACT: To study the correlation of OX40 Gene rs17568A/G polymorphism with acute coronary syndrome in Chinese Han population.
228 ACS patients and 165 matched controls of Chinese Han population were selected to study the OX40 gene rs17568A/G polymorphism with PCR-RFLP method. DNA sequences of enzyme digested products were also analyzed.
All three genotypes, AA, AG and GG, were existed both in ACS patients and control group. OX40 gene rs17568A/G polymorphism frequencies were 29.4% for patients and 26.7% for control group respectively, in which the statistic significance was not observed (P > 0.05). Besides, no significant differences were found for G allele and genotype frequencies in different severity of coronary lesion. But the results demonstrated that the presence of G allele was associated with high density lipoprotein (HDL) cholesterol concentration in either patients or controls.
OX40 gene rs17568A/G polymorphism was not associated with acute coronary syndrome in Chinese population, however, this polymorphism has some influence on serum HDL-C level in Chinese population.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 08/2008; 39(4):601-4.
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ABSTRACT: Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-time reverse transcription-polymerase chain reaction and western blotting. The angiogenic activity in vitro was evaluated by transwell migration assay and Matrigel-induced capillary tube formation in hypoxic culture. Cell proliferation was determined by crystal violet staining. Results The results showed that levels of the mini-TyrRS/mini-TrpRS gene and protein in mock transfection group and negative control group were higher, but noticeably decreased in experimental group. However, no significant difference was detected between mock transfection group and negative control group, but there was a statistically significant difference compared with experimental group. For mini-TyrRS-siRNA group, the cell migration, tube formation and the rate of cell proliferation were respectively inhibited by (47.4, 56.3, 65.4, 73.7%), (60.5, 69.1, 75.9, 83.6%) and (40.4, 56.2, 61.2, 68.0%). For mini-TrpRS-siRNA, were respectively increased by (18.0, 33.8, 45.1, 56.4%), (18.3, 31.2, 40.3, 45.7%) and (8.4, 26.4, 38.2, 46.6%). Conclusion These results indicated that angiogenesis is either stimulated by mini-TyrRS or inhibited by mini-TrpRS in matrigel models in hypoxic culture, raising the possibility that mini-TyrRS stimulates a common downstream signaling event. Thus, naturally occurring fragments of two proteins involved in translation, TyrRS and TrpRS, have opposing activity on endothelial cell angiogenesis in the matrigel assays. The opposing activities of the two tRNA synthetases suggest tight regulation of the balance between pro- and anti-angiogenic stimuli.
Cytotechnology 04/2008; 56(3):219-31. · 1.21 Impact Factor
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ABSTRACT: To investigate the cholesterol 7alpha-hydroxylase gene -204A/C polymorphism and its relationship with serum lipids and apolipoproteins (apo) levels in patients with endogenous hypertriglyceridemia (HTG) in Chinese population in Chengdu area.
The genotype and allele frequencies of cholesterol 7alpha-hydroxylase gene -204A/C polymorphism were analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Serum lipids were measured by enzymatic kits and apolipoproteins AI, AII, B100, CII, CIII and E were measured by the RID kits in 132 HTG patients and 212 control subjects.
Allele frequencies of A and C were 0.602 and 0.398 in HTG group and 0.601 and 0.399 in control group, respectively. There was no significant difference of allele and genotypes frequencies between HTG and control groups (P> 0.05). In HTG group, carriers with the genotypes CC and AC were associated with significantly higher concentrations of triglycerides and apoCIII compared with those with genotype AA (P< 0.05). In the control group, carriers with the genotypes CC and AC were associated with significantly lower serum high density lipoprotein cholesterol (HDL-C) level compared with those with genotype AA (P< 0.05). In the male control group, carriers with the genotypes CC and AC had elevated levels of serum triglycerides than those with genotype AA (P< 0.05).
These results suggest that -204A/C polymorphism in the CYP7A1 gene does not relate with HTG but may has an effect on serum triglyceride and apoCIII levels in patients with endogenous HTG, the serum HDL-C level in control subjects and the serum TG level in male control subjects.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 08/2007; 24(4):432-6.
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ABSTRACT: To evaluate the effects of selective and non-selective cyclooxygenase-2 on the growth and apoptosis of colon cancer cell lines in vitro.
The proliferation of colon cancer cells was determined by MTT assay, and the cell cycle progression was analyzed by flow cytometric assay. Annexin V/PI staining in combination with flow cytometric assay was used to detect apoptosis induced by NSAIDs.
It was found that celecoxib, meloxicam and aspirin could inhibit the growth of HT-29 or SW480 cell and showed a concentration dependent pattern. COX-2 protein was expressed in HT-29 and LS174-T, but not in SW480 cells. In addition, PCNA levels in both HT-29 cell and SW480 cells were reduced by aspirin and celecoxib. Both aspirin (10 mmol/ L) and celecoxib (50 micromol/L) induced apoptosis of HT-29 and SW480 colon cancer cells, the apoptosis rates were 4.8%, 17.7% and 5.1%, 20.4% respectively.
Both COX-2 selective and non-selective inhibitors can potentially inhibit the growth of colon cell lines and such inhibitory effect on COX-2 negative colon cancer cells is also evident.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 08/2006; 37(4):547-50.
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ABSTRACT: To investigate the effect of vasoactive intestinal polypeptide (VIP) on differentiation of hematopoietic stem cells (HSC) into hepatic related cells and probe into the possibility that VIP affects HSC transdifferentiation.
Mini MACS assay was used to purify human CD34+ cells from mononuclear cell (MNC), the purity of the CD34+ cells was evaluated by flow cytometry. Alpha fetal proteins(AFP) in cultured CD34+ cells and their supernatant were measured with ELISA assay. Liver tissue markers on CD34+ cells, AFP, albumin (ALB) and CK-19, were measured by immunohistochemistry. Western blot assay was used to detect the expression of ALB on CD34+ cells. Nest RT-PCR was used to measure the expression of AFP mRNA and ALB mRNA on CD34+ cells, the product of ALB was chosen to measure the sequence.
Immunohistochemistry showed that human CD34+ cell contained AFP and albumin but no CK-19 protein. When human CD34+ cells were cultured with VIP for 14 days, the concentration of AFP within CD34+ cells was decreased from (270.00 +/- 11.37) pg/mL to (165.00 +/- 8.51) pg/mL (P < 0.05). Western blot revealed that albumin in CD34+ cells treated with VIP faded, compared to that of control. Both human cord blood MNC and CD34+ cells expressed AFP mRNA and albumin mRNA; the sequence of the product of nest RT-PCR for albumin matched completely with the sequence of albumin in GenBank.
Human cord blood CD34+ cells have been note to express hepatocyte related markers such as AFP and albumin, although there is no evidence of CK-19 expression. These findings suggests the possibility that human HSC could be transdifferentiated into hepatocyte. The expressions of AFP and albumin on human CD34+ cells depressed by VIP indicate that VIP might inhibit the transdifferentiation of HSC.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 07/2006; 37(4):578-82.
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ABSTRACT: This study was aimed to assess the effects of somatostatin (SST) and its analog Octreotide on the treatment of acute respiratory distress syndrome (ARDS) in rat model.
ARDS rat model was induced by administration of paraquat. After 24 hours oral administration of paraquat, three drugs (SST, Octreotide and dexamethasone at dosage of 1 mg/kg, 0.1 mg/kg and 1 mg/kg respectively) were infused into the tail veins of rats so as to form three treatment groups. The rats given only paraquat were subjects in the ARDS group. PaO2 level and pulmonary coefficient were detected, and the morphological changes of lungs were examined. In addition, TNF-alpha and IL-6 levels in plasma, Bronchoalveolar lavage fluids (BALF) and intestine homogenate were measured respectively.
The ARDS rats treated with SST, Octreotide and dexamethasone showed mitigation of inflammatory response in the lungs, improvement in lung ventilation, reduction of pulmonary coefficient (-13%-15%), increase in PaO2 level (+75%-84%), decrease in PaCO2 level (-17%-22%), and restoration of pH value to normal in plasma, when compared with the rats of ARDS group. TNF-alpha level in plasma tended to decline (P>0.05); it significantly lowered in BALF and intestine homogenate in the ARDS rats treated (P< 0.05). IL-6 level in BALF tended to be lower in treatment group (P>0.05), but the IL-6 level in plasma and intestine homogenate displayed no significant difference between the treatment group and ARDS group.
These results suggest that SST and its analog Octreotide assume a role in improving the clinical manifestation of ARDS, alleviating the inflammatory condition of the lungs, and reducing the TNF-alpha level in the BALF and intestine homogenate, therefore they may be useful adjuvants in the treatment of ARDS.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 05/2006; 37(3):412-5.
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ABSTRACT: Accumulated data have suggested that vasoactive intestinal polypeptide (VIP) and corresponding receptor (VIPR) are involved in the development of hematopoietic stem cells and liver growth. In the present study, radioimmunoassay, biomolecular interaction analysis and reverse transcriptation polymerase chain reaction were used to quantify VIP, VIPR and detect the subtype of VIPR in rat liver during development. VIP concentration of liver in fetal or neonatal rats was significantly lower than that of teens or adult rats (P<0.05). The binding capacities of VIPR in liver of immature rats were much greater than that of the adult rats (P<0.05). The tendency of change in VIP concentration was contrary to that of the binding capacity of VIPR in the liver of rats during development. VIPR-1 was expressed in rat liver in all phases of development. These results may be of benefit to the understanding of the mechanisms of liver growth and fetal liver hemopoiesis shift.
Sheng li xue bao: [Acta physiologica Sinica] 07/2005; 57(3):379-83.
