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ABSTRACT: To study the effect on anti-respiratory syncytial virus of an active compound GD4 from Glycyrrhiza in vitro.
GD4 was extracted from Glycyrrhiza by hot water extraction, dichloromethane extraction and column chromatography. The CPE inhibition assay was used to test the antiviral activity of GD4 on RSV in Hela cells.
GD4 was effective antiviral agent for RSV in a concentration-dependent manner and its median toxic concentration (TC50) and median effeicacious concentration (EC50) and treatment index (TI) was 0.23 mg/ml, 28.73 microg/ml and 8.0. 120 microg/ml GD4 exhibited inhibitory effect when added into cell culture for every 2h within 12h post-infection. GD4 didn't contain glycyrrhizic acid.
The active compound of Glycyrrhiza (GD4) was an evident inhibitory effect on RSV.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 08/2006; 29(7):692-4.