Wei Gong

Nanjing Medical University, Nanjing, Jiangsu Sheng, China

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Publications (3)3.14 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: To investigate the effects of Flunarizine (Flu) on T-type calcium currents (ICaT) in spermatogenic cells. Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed. Flu of 0.1, 1, 10, 100 micromol/L inhibited ICaT in mouse spermatogenic cells significantly with the K50 value of 0.289 micromol/L (P < 0.05). With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were (23.34 +/- 2.76)%, (46.04 +/- 3.52)%, (62.52 +/- 3.70)% and (73.52 +/- 3.12)%, respectively. Flu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca(v)3.2 is the main contributor to T-type Ca2+ currents in spermatogenic cells.
    Zhonghua nan ke xue = National journal of andrology 07/2006; 12(7):594-7, 601.
  • [show abstract] [hide abstract]
    ABSTRACT: Prior to fertilization sperm must undergo the acrosome reaction that is initiated by opening of T-type Ca(2+) channel. Hence, T-type Ca(2+) channels could be a potential target for agents affecting the acrosome reaction. Our previous data have suggested that fenvalerate, a type II pyrethroid, inhibited the acrosome reaction in mice. To elucidate its potential mechanism we investigated fenvalerate's effect on T-type Ca(2+) channels in mouse pachytene spermatocytes using a whole-cell patch clamp technique. Fenvalerate significantly inhibited T-type Ca(2+) currents in a concentration-dependent manner. The maximal inhibitory concentration was 1 microM. The inhibitory effect of fenvalerate was slow and irreversible after washout of the drug. The curves of activation and inactivation simultaneously shifted to hyperpolarization, about 14 mV, suggesting the open time of Ca(2+) channel was unchanged. Voltage-dependent gating of Ca(2+) channel indicated a change in permeability to ions that contributed to fenvalerate's inhibition on Ca(2+) current. Taken together with our previous findings, these data suggest that the changes of T-type Ca(2+) currents contribute to the suppression of acrosome reaction and fertilizing ability caused by fenvalerate.
    Reproductive Toxicology 02/2006; 21(1):48-53. · 3.14 Impact Factor
  • Wei Gong, Hang Xiao
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    ABSTRACT: The physiological changes that occur to sperm during the residence in the female tract are collectively referred to as "capacitation". The mechanism of action by which these compounds promote capacitation is poorly understood at the molecular level. However, some molecular events significant to the initiation of capacitation have been identified, such as the correlation of capacitation with cholesterol efflux from the sperm plasma membrane, increased membrane fluidity, modulations in intracellular ion concentrations, hyperpolarization of the sperm plasma membrane and increased protein tyrosine phosphorylation. This review discusses recent progress in elucidation mechanisms which regulate sperm capacitation.
    Zhonghua nan ke xue = National journal of andrology 01/2004; 9(9):693-6.