W. Wang

Shandong University, Chi-nan-shih, Shandong Sheng, China

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Publications (90)146.91 Total impact

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    ABSTRACT: Liver ischemia-reperfusion (I/R) injury is of great importance in primary graft dysfunction after transplantation, and could be more severe in transplantation using aged donor livers. In order to alleviate the I/R injury in aged donor livers, we transferred exogenous human telomerase reverse transcriptase (hTERT) gene into aged rat's livers before liver transplantation. After transplantation, the effect of the gene for aged rats on cell apoptosis caused by I/R injury was evaluated.
    Transplantation Proceedings 05/2014; · 0.95 Impact Factor
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    M Li, L Wang, W Wang, X L Qi, Z Y Tang
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    ABSTRACT: Iron homeostasis dysregulation has been regarded as an important mechanism in neurodegenerative diseases. The H63D and C282Y polymorphisms in the HFE gene may be involved in the development of sporadic amyotrophic lateral sclerosis (ALS) through the disruption of iron homeostasis. However, studies investigating the relationship between ALS and these two polymorphisms have yielded contradictory outcomes. We performed a meta-analysis to assess the roles of the H63D and C282Y polymorphisms of HFE in ALS susceptibility. PubMed, MEDLINE, EMBASE, and Cochrane Library databases were systematically searched to identify relevant studies. Strict selection criteria and exclusion criteria were applied. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of associations. A fixed- or random-effect model was selected, depending on the results of the heterogeneity test. Fourteen studies were included in the meta-analysis (six studies with 1692 cases and 8359 controls for C282Y; 14 studies with 5849 cases and 13,710 controls for H63D). For the C282Y polymorphism, significant associations were observed in the allele model (Y vs C: OR=0.76, 95%CI=0.62-0.92, P=0.005) and the dominant model (YY+CY vs CC: OR=0.75, 95%CI=0.61-0.92, P=0.006). No associations were found for any genetic model for the H63D polymorphism. The C282Y polymorphism in HFE could be a potential protective factor for ALS in Caucasians. However, the H63D polymorphism does not appear to be associated with ALS.
    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas / Sociedade Brasileira de Biofisica ... [et al.] 02/2014; · 1.08 Impact Factor
  • L. Yu, C. Liu, W. Zhu, S. Hua, W. Wang
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    ABSTRACT: Video delivery in television white space (TVWS) is a challenging task due to the variation of the spectrum availability in TVWS and the stringent bandwidth requirements of video streaming. Most existing frameworks employ an opportunistic spectrum access style which result in bandwidth inefficiency since only one spectrum hole in TVWS can be used. In this paper, we propose a bandwidth efficient TVWS video delivery framework. First, a multisector structure is employed to improve the bandwidth efficiency. Second, a novel spectrum sensing scheme and an efficient spectrum selection algorithm are proposed to obtain the optimal spectrum holes based on this structure. Third, a joint optimization approach is proposed to create an optimal map between the sectors and the spectrum, and to adapt the video rate commensurate with dynamic channel conditions. The simulation results demonstrate that the proposed framework can obtain significantly higher bandwidth efficiency and superior video performance than traditional approaches.
    IEEE Transactions on Circuits and Systems for Video Technology 01/2014; 24(9):1605-1619. · 1.82 Impact Factor
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    ABSTRACT: To investigate the feasibility of pre- and postoperative gemcitabine-plus-cisplatin (GC) adjuvant chemotherapy for the treatment of locally advanced urothelial carcinoma in kidney transplant patients. Seven kidney transplant patients diagnosed with locally advanced urothelial carcinoma were treated with a pre- and postoperative GC adjuvant chemotherapy between January 2008 and March 2012. Gemcitabine (800 mg/m(2)) was administered at as an intravenous infusion on days 1 and 8. A total cisplatin dosage of 100 mg/cycle was administered on 2 days (50 mg/d on days 2 and 3) as an intravenous infusion. A single treatment cycle lasted 21 days. At the beginning of chemotherapy, the cyclosporine (CSA) dosage was reduced by 25 mg/d (on day 1 through day 8) if the blood CSA concentration was well maintained and did not fluctuate significantly. In addition, mycophenolate mofetil was reduced by 500 mg/d, while azathioprine was reduced by 25 mg/d (on day 1 through day 16). One cycle of GC neoadjuvant chemotherapy was given before operation, and several GC cycles were given after operation according to the patients' situation. Retrospective analysis was performed on the clinical data, chemotherapy regimen, chemotherapy efficacy, and side effects of the 7 patients. The 7 patients were all treated with 1 course of presurgical chemotherapy. The seven patients completed 24 treatment cycles of chemotherapy in total. The average GC medication period per patient was 3.4 cycles. The postsurgery follow-up was 6 to 36 months (average-22.1); all of the patients survived. There was 1 case of complete remission (14.5%), 2 of partial remission (28.5%), and 4 of stable disease (57%), with one case of T4N1M0 and three cases of T3N0M0. The overall efficacy was 43%. The toxicity and side effects associated with the GC regimen were largely associated with myelosuppression. The other side effects included reversible nephrotoxicity, gastrointestinal tract and skin reactions, as well as phlebitis. Hematologic toxic reactions included reversible leukopenia, thrombocytopenia, and anemia. There was 1 case of degree III anemia and 1 case of degree II; 5 cases of degree III and 1 of degree II leukopenia; and 3 of degree II thrombocytopenia. Gastrointestinal reactions included nausea, vomiting, and constipation. There were 2 cases of degree III and 4 cases of degree II nausea and vomiting as well as 2 cases of degree III and 3 cases of degree II constipation. There were 3 cases of degree I phlebitis (43%) and 2 cases of degree I skin erythema. The nephrotoxicity reactions were all reversible. Both liver function and grafted kidney function were not significantly altered after chemotherapy compared with prior to chemotherapy. None of the patients suffered renal allograft rejection after chemotherapy; none required additional antirejection drug treatments. The original antirejection treatment regimen was restored after the patients completed the chemotherapy treatment cycles. We confirmed the efficacy of applying a GC regimen to treat locally advanced urothelial carcinoma in kidney transplant patients. The side effects were tolerable and reversible with minor impacts on graft function.
    Transplantation Proceedings 11/2013; 45(9):3293-7. · 0.95 Impact Factor
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    ABSTRACT: We describe the design and implementation of Climate Fast Input/Output (CFIO), a fast input/output (I/O) library for high resolution climate models. CFIO provides a simple method for modelers to overlap the I/O phase with the computing phase automatically, so as to shorten the running time of numerical simulations. To minimize the code modifications required for porting, CFIO provides similar interfaces and features to Parallel network Common Data Form (PnetCDF), which is one of the most widely used I/O libraries in climate models. We deployed CFIO in three high resolution climate models, including two ocean models (POP and LICOM) and one sea ice model (CICE). The experimental results show that CFIO improves the performance of climate models significantly versus the original serial I/O approach. When running with CFIO at 0.1° resolution with about 1000 CPU cores, we managed to reduce the running time by factors of 7.9, 4.6 and 2.0 for POP, CICE, and LICOM respectively. We also compared the performance of CFIO against PnetCDF in different scenarios. For scenarios with both data output operations and computations, CFIO decreases the I/O overhead by a factor of 5.1 compared to PnetCDF.
    Geoscientific Model Development Discussions 09/2013; 6(3):4775-4807.
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    ABSTRACT: Immunotherapy that is based on adoptive transfer of T lymphocytes, which are genetically modified to express chimeric antigen receptors (CARs) that recognize tumor-associated antigens, has been demonstrated to be an efficient cancer therapy. Vascular endothelial growth factor receptor-1 (VEGFR-1), a vital molecule involved in tumor growth and angiogenesis, has not been targeted by CAR-modified T lymphocytes. In this study, we generated CAR-modified T lymphocytes with human VEGFR-1 specificity (V-1 CAR) by electroporation. V-1 CAR-modified T lymphocytes were demonstrated to elicit lytic cytotoxicity to target cells in a VEGFR-1-dependent manner. The adoptive transfer of V-1 CAR T lymphocytes delayed tumor growth and formation and inhibited pulmonary metastasis in xenograft models and such efficacies were enhanced by cotransfer of T lymphocytes that expressed interleukin-15 (IL-15). Moreover, V-1 CAR-modified T lymphocytes lysed primary endothelial cells and impaired tube formation, in vitro. These data demonstrated the antitumor and anti-angiogenesis ability of V-1 CAR-modified T lymphocytes. Our study provides the rationale for the clinical translation of CAR-modified T lymphocytes with VEGFR-1 specificity.Gene Therapy advance online publication, 2 May 2013; doi:10.1038/gt.2013.19.
    Gene therapy 05/2013; · 4.75 Impact Factor
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    ABSTRACT: Chronic infection, such as Helicobacter pylori infection, has been associated with the development of gastric cancer (GC). Pathogen-associated molecular patterns can trigger inflammatory responses via Toll-like receptors (TLRs) in GC. Here we showed that Toll-like receptor 4 (TLR4) was highly expressed in GC cells and was associated with the aggressiveness of GC. The binding of lipopolysaccharide (LPS) to TLR4 on GC cells enhanced proliferation without affecting apoptosis. Higher level of reactive oxygen species (ROS) was induced after activation of TLR4 signaling in GC. Using oxidase inhibitors and antioxidants, we found that mitochondrial ROS (mROS) was major source of TLR4-stimulated ROS generation. This elevated mROS production can be inhibited by diphenylene iodonium (DPI), and the blocking of the mROS production rather than ROS neutralization resulted in cell cycle arrest and the loss of mitochondrial potential, which were plausible reason for decreased cell viability. Furthermore, the increased mROS owing to TLR4 signaling resulted in the activation of Akt phosphorylation and NF-κB p65 nuclear translocation. Altogether, these results reveal a novel pathway linking innate immune signaling to GC cell proliferation, implicate mROS as an important component of cell survival signals and further establish mitochondria as hubs for GC therapies.
    Cell Death & Disease 01/2013; 4:e794. · 6.04 Impact Factor
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    ABSTRACT: In this paper, we present a community-detection solution for massive-scale social networks using MapReduce, a parallel programming framework. We use a similarity metric to model the community probability, and the model is designed to be parallelizable and scalable in the MapReduce framework. More importantly, we propose a set of degree-based preprocessing and postprocessing techniques named DEPOLD (DElayed Processing of Large Degree nodes) that significantly improve both the community-detection accuracy and performance. With DEPOLD, delaying analysis of 1% of high-degree nodes to the postprocessing stage reduces both processing time and storage space by one order of magnitude. DEPOLD can be applied to other graph-clustering problems. Furthermore, we design and implement two similarity calculation algorithms using MapReduce with different computation and communication characteristics in order to adapt to various system configurations. Finally, we conduct experiments with publicly available datasets. Our evaluation demonstrates the effectiveness, efficiency, and scalability of the proposed solution.
    Ibm Journal of Research and Development 01/2013; 57(3/4):12:1-12:14. · 0.69 Impact Factor
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    ABSTRACT: Abnormal expression of microRNAs (miRNAs) has been implicated in carcinogenesis. Here we report a novel BCR (breakpoint cluster region)-ABL (c-abl oncogene 1, non-receptor tyrosine kinase)/GATA1/microRNA-138 (miR-138) circuitry in chronic myeloid leukemia (CML). miR-138 expression is downregulated in K562 cells and primary CML samples, which is restored after imatinib treatment. The tumor suppressor activity of miR-138 is demonstrated by the induction of cell cycle arrest at G0/G1, inhibition of cell proliferation and colony forming unit granulocyte-macrophage colony formation and enhanced imatinib-induced apoptosis in K562 and Ku812 cells overexpressing miR-138. Moreover, overexpression of miR-138 led to the downregulation of BCR-ABL. Based on luciferase assay, ABL and BCR-ABL are shown to be the target genes regulated by miR-138. Furthermore, miR-138 binding to ABL was shown to localize to the coding region instead of 3'-untranslated regions (3'-UTR) of ABL mRNA. In addition, CCND3 is another target of miR-138, which represses CCND3 expression by binding to its 3'-UTR. Finally, upregulation of miR-138 upon imatinib treatment is associated with the enhancement of GATA1 activity, which binds to the miR-138 promoter. In conclusion, miR-138 is a tumor suppressor miRNA underexpressed in CML. miR-138 represses expression of both BCR-ABL and CCND3 via binding to the coding region and 3'-UTR, respectively. miR-138 expression is activated by GATA1, which in turn is repressed by BCR-ABL. Therefore, miR-138, by virtue of a BCR-ABL/GATA1/miR-138 circuitry, is a tumor suppressor miRNA implicated in the pathogenesis of CML and its clinical response to imatinib.Oncogene advance online publication, 3 December 2012; doi:10.1038/onc.2012.557.
    Oncogene 12/2012; · 8.56 Impact Factor
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    ABSTRACT: Objectives: Myeloid-derived suppressor cells (MDSCs) have recently been identified as an important mediator in inflammatory and autoimmune diseases through the production of arginase (Arg)-1 and inducible nitric oxide synthase (iNOS). The aim of this study was to investigate the prevalence of MDSCs in the peripheral blood of patients with rheumatoid arthritis (RA) and evaluate their correlation with T-helper (Th)17 cells. Method: The frequency of MDSCs and Th17 cells and the mRNA expression of transcriptional factor RORγ-t and iNOS in the peripheral blood of RA patients and healthy controls (HC) were determined by flow cytometry and real-time reverse transcription polymerase chain reaction (RT-PCR), respectively. Plasma levels of interleukin (IL)-17, IL-6, tumour necrosis factor (TNF)-α, and Arg-1 were analysed by enzyme-linked immunosorbent assays (ELISA). Results: Compared with HC, both the prevalence of circulating MDSCs and plasma Arg-1 increased significantly in RA patients. However, no significant difference was observed in the mRNA level of iNOS between RA patients and HC. The frequency of Th17 cells in RA patients was significantly higher than in HC but correlated negatively with the frequency of MDSCs and plasma Arg-1. A negative correlation between MDSCs and plasma TNF-α was also observed. However, the frequency of MDSCs was not correlated with plasma IL-6 and IL-17, nor with the mRNA level of RORγ-t. Conclusions: We found a negative correlation between increased circulating MDSCs and Th17 cells in RA patients, which may provide new insights into the mechanisms involved in RA.
    Scandinavian journal of rheumatology 11/2012; · 2.51 Impact Factor
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    ABSTRACT: SUMMARY Femto cell technology is a promising solution for indoor coverage of cellular systems. The interference between macro and femto cells can be mitigated via cooperation between the macro base station (BS) and the inside femto sites (FSs). In this paper, the idea of multi-cell multi-input and multi-output is introduced, whereby the macro BS shares the same frequency band with the inside FSs in support of the femto users. Both single-user and multi-user precoding at the macro BS are proposed to support the cooperative transmission between the macro BSs and FSs. In single-user precoding and multi-user precoding without power allocation, only the angle information of the FS-user channels is required to be sent from the users to the macro BS. If the magnitude information is also sent by each user, multi-user precoding with power allocation can be employed to support cooperation between macro BSs and FSs, which is an extension of the classical water-filling optimization problem. Theoretical derivations and an iterative algorithm are both presented to solve this optimization problem. Analytical and simulation results with respect to the signal received with interferences validate the effectiveness of cooperation between the macros BSs and FSs.Copyright © 2012 John Wiley & Sons, Ltd.
    International Journal of Communication Systems 10/2012; · 1.11 Impact Factor
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    ABSTRACT: Abstract The objective of this study was to investigate the effects of single nucleotide polymorphisms (SNPs) in nine candidate genes on meat quality traits in longissimus dorsi muscle of pigs. Each SNP allele's main effects were as follows: ADRB3 c. 1192G allele increased intramuscular fat (IMF; p<0.01) and yellowness (p<0.05). MC4R c. 745G allele increased body weight (p<0.05). LEPR c. 232T allele increased rib eye area and decreased tenderness (p<0.05). HSL c. 873G allele increased rib eye area and decreased IMF and backfat thickness (p<0.05). LPL c. 74T allele increased IMF, tenderness, and yellowness (p<0.05). MYF5 c. 1205A allele improved tenderness (p<0.05). H-FABP c. 1322T allele improved tenderness and yellowness (p<0.05). PPAR? c. 175G allele decreased water loss (p<0.05). The identified genetic markers might be useful for evaluation and utilization of meat quality related gene in the effort to improve porcine meat quality.
    Acta Agriculturae Scandinavica, Section A – Animal Science 09/2012; 62(3):120-126. · 0.61 Impact Factor
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    ABSTRACT: Background Oxaliplatin-related neurotoxicity is the main limitation for its continuation in adjuvant and palliative chemotherapy for patients with colorectal cancer. The purpose of this meta-analysis was to determine the efficacy of calcium and magnesium (Ca/Mg) infusions in oxaliplatin-induced neurotoxicity.Methods Two independent authors conducted database searches of the literature to find clinical-controlled trials analyzing Ca/Mg infusions in oxaliplatin-induced neurotoxicity. The keywords used to search were oxaliplatin, neurotoxicity, calcium, magnesium, neuropathy, and peripheral. Clinical studies that included at least one primary or secondary event were eligible for the analysis, where primary events were incidences of acute and cumulative neurotoxicity, and secondary events were the total doses and cycles of oxaliplatin, response rate (RR), overall survival (OS), and progression-free survival (PFS). Odds ratios (ORs) and weighted mean differences (MD) were analyzed using models of fixed and random effects.ResultsThis meta-analysis comprised four prospective randomized clinical trials and three retrospective clinical trials involving 1170 colorectal cancer patients, of which 802 received Ca/Mg infusions (Ca/Mg group) and 368 did not (control group). According to the National Cancer Institute-Common Terminology Criteria for Adverse Events, the incidence of grade 3 acute neurotoxicity in those who received Ca/Mg was significantly lower than that of the control group (OR = 0.26; 95% confidence interval (CI), 0.11 to 0.62; P = 0.0002). The total rate of cumulative neurotoxicity, and that of grade 3 in particular, was significantly lower in the Ca/Mg group than in the control group (OR = 0.42; 95% CI 0.26-0.65; P = 0.0001; OR = 0.60; 95% CI 0.39-0.92; P = 0.02, respectively). The differences in total doses and cycles of oxaliplatin were also significant between the Ca/Mg and control group (MD = 246.73 mg/m(2); 95% CI 3.01-490.45; P = 0.05; MD = 1.55; 95% CI 0.46-2.63; P = 0.005, respectively). No significant differences were found in median PFS (MD = 0.71 month; 95% CI -0.59-2.01; P = 0.29), median OS (MD = 0.10 month; 95% CI -0.41-0.61; P = 0.70) or RRs (OR = 0.82; 95% CI 0.61-1.10; P = 0.18).Conclusion Ca/Mg infusions tend to decrease the incidence of oxaliplatin-induced acute and cumulative neurotoxicity and thus enhance patients' tolerance to oxaliplatin, without significantly altering the efficacy of chemotherapy.
    Annals of Oncology 08/2012; · 7.38 Impact Factor
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    ABSTRACT: Liver cancer is the fifth most common cause of cancer deaths worldwide. Noninvasive diagnosis is difficult and the disease heterogeneity reduces the accuracy of pathological assays. Improvement in diagnostic imaging of specific molecular disease markers has provided hope for accurate and early noninvasive detection of liver cancer. However, all current imaging technologies, including ultrasonography, computed tomography (CT), positron emission tomography (PET), and magnetic resonance imaging, are not specific targets for detection of liver cancer. The aim of this study was to test the feasibility of injecting a cocktail of specific molecular imaging agents to noninvasively image liver cancer. The target-specific cocktail contained agents for imaging the neovasculature (RGD peptide), matrix metalloproteinase (MMP), and glucose transport (18F-fluorodeoxyglucose [18F-FDG]). Imaging studies were performed in liver cancer cells and xenograft models. The distribution of MMP at the intracellular level was imaged by confocal microscopy. RGD, MMP, and 18F-FDG were imaged on tumor-bearing mice using PET, CT, X-ray, and multi-wavelength optical imaging modalities. Image data demonstrated that each agent bound to a specific disease target component. The same liver cancer xenograft contained multiple disease markers. Those disease markers were heterogenetically distributed in the same tumor nodule. The molecular imaging agents had different distributions in the whole body and inside the tumor nodule. All target-specific agents yielded high tumor-to-background ratios after injection. In conclusion, target-specific molecular imaging agents can be used to study liver cancer in vitro and in vivo. Noninvasive multimodal/multi-target-specific molecular imaging agents could provide tools to simultaneously study multiple liver cancer components.
    Current Molecular Medicine 07/2012; 12(8):944-51. · 4.20 Impact Factor
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    ABSTRACT: A novel data-model-fusion prognostic framework is developed in this paper to improve the accuracy of system state long-horizon forecasting. This framework strategically integrates the strengths of the data-driven prognostic method and the model-based particle filtering approach in system state prediction while alleviating their limitations. In the proposed methodology, particle filtering is applied for system state estimation in parallel with parameter identification of the prediction model (with unknown parameters) based on Bayesian learning. Simultaneously, a data-driven predictor is employed to learn the system degradation pattern from history data so as to predict system evolution (or future measurements). An innovative feature of the proposed fusion prognostic framework is that the predicted measurements (with uncertainties) from the data-driven predictor will be properly managed and utilized by the particle filtering to further update the prediction model parameters, thereby enabling markedly better prognosis as well as improved forecasting transparency. As an application example, the developed fusion prognostic framework is employed to predict the remaining useful life of lithium ion batteries through electrochemical impedance spectroscopy tests. The investigation results demonstrate that the proposed fusion prognostic framework is an effective forecasting tool that can integrate the strengths of both the data-driven method and the particle filtering approach to achieve more accurate state forecasting.
    Engineering Applications of Artificial Intelligence 06/2012; 25(4):814–823. · 1.96 Impact Factor
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    ABSTRACT: BACKGROUND: The role of naturally occurring regulatory T cells (Treg) in the control of the development of systemic lupus erythematosus (SLE) has not been well defined. Therefore, we dissect the phenotypically heterogeneous CD4(+)FoxP3(+) T cells into subpopulations during the dynamic SLE development. METHODLOGY/PRINCIPAL FINDINGS: To evaluate the proliferative and suppressive capacities of different CD4(+) T cell subgroups between active SLE patients and healthy donors, we employed CD45RA and CD25 as surface markers and carboxyfluorescein diacetatesuccinimidyl ester (CFSE) dilution assay. In addition, multiplex cytokines expression in active SLE patients was assessed using Luminex assay. Here, we showed a significant increase in the frequency of CD45RA(+)FoxP3(low) naive Treg cells (nTreg cells) and CD45RA(-)FoxP3(low) (non-Treg) cells in patients with active SLE. In active SLE patients, the increased proportions of CD45RA(+)FoxP3(low) nTreg cells were positively correlated with the disease based on SLE disease activity index (SLEDAI) and the status of serum anti-dsDNA antibodies. We found that the surface marker combination of CD25(+)CD45RA(+) can be used to defined CD45RA(+)FoxP3(low) nTreg cells for functional assays, wherein nTreg cells from active SLE patients demonstrated defective suppression function. A significant correlation was observed between inflammatory cytokines, such as IL-6, IL-12 and TNFα, and the frequency of nTreg cells. Furthermore, the CD45RA(+)FoxP3(low) nTreg cell subset increased when cultured with SLE serum compared to healthy donor serum, suggesting that the elevated inflammatory cytokines of SLE serum may promote nTreg cell proliferation/expansion. CONCLUSIONS/SIGNIFICANCE: Our results indicate that impaired numbers of functional CD45RA(+)FoxP3(low) naive Treg cell and CD45RA(-)FoxP3(low) non-suppressive T cell subsets in inflammatory conditions may contribute to SLE development. Therefore, analysis of subsets of FoxP3(+) T cells, using a combination of FoxP3, CD25 and CD45RA, rather than whole FoxP3(+) T cells, will help us to better understand the pathogenesis of SLE and may lead to the development of new therapeutic strategies.
    PLoS ONE 04/2012; · 3.53 Impact Factor
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    ABSTRACT: Cancer stem cells (CSCs) paradigm suggests that CSCs might have important clinical implications in cancer therapy. Previously, we reported that accumulation efficiency of CSCs is different post low- and high-LET irradiation in 48 h. Cancer stem cells and non-stem cancer cells (NSCCs) were sorted and functionally identified through a variety of assays such as antigen profiles and sphere formation. Inter-conversion between CSCs and NSCCs were in situ visualised. Cancer stem cells proportions were assayed over multiple generations under normal and irradiation surroundings. Supplement and inhibition of TGF-β1, as well as immunofluorescence assay of E-cadherin and Vimentin, were performed. Surface antigen markers of CSCs and NSCCs exist in an intrinsic homoeostasis state with spontaneous and in situ visualisable inter-conversions, irrespective of prior radiations. Supplement with TGF-β1 accelerates the equilibrium, whereas inhibition of TGF-β signalling disturbs the equilibrium and significantly decreases CSC proportion. Epithelial mesenchymal transition (EMT) might be activated during the process. Our results indicate that the intrinsic inter-conversion and dynamic equilibrium between CSCs and NSCCs exist under normal and irradiation surroundings, and TGF-β might have important roles in the equilibrium through activating EMT.
    British Journal of Cancer 04/2012; 106(9):1512-9. · 5.08 Impact Factor
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    ABSTRACT: To avoid the misdiagnosis of prostate cancer (PCA), many patients receive repeated biopsies, despite receiving prior negative biopsies for PCA. Signal transduction and activators of transcription 3 (STAT3), a component of the JAK-STAT signaling pathway, can be activated by tyrosine phosphorylation as P-STAT3 and involved in the regulation of cellular growth, survival and oncogenesis. We aimed to assess the reliability of detecting PCA from the expression of P-STAT3 in prostate tissue previously designated as a negative biopsy. Prostate tissues were obtained from the biopsies of 52 patients with localized PCA as well as from the biopsies of 80 patients free of PCA. Expression of P-STAT3 in these specimens was examined by immunohistochemical staining (IHC) and used to distinguish tissue with PCA from tissue designated as benign during a biopsy procedure. P-STAT3 staining intensities in all samples (initial negative biopsies, cancer positive cores and other negative cores from the same-batch biopsies) of PCA patients was significantly higher than that of benign patients (F = 23.664, P < 0.001). Analysis of the receiver operating characteristics (ROC) curve showed that the area under curve (AUC) for P-STAT3 staining was 0.785. When positive immuno-labeling of P-STAT3 in samples from initial biopsies was used as a marker for PCA, it showed relatively high sensitivity (80.8%) and specificity (76.3%). IHC of P-STAT3 could be utilized to detect PCA patients with initial negative biopsies. As a result, it can be a potential adjunctive tool for current PCA diagnostic programs. P-STAT3 can predict the onset of PCA up to 40 months earlier than currently used diagnostic approaches.
    European journal of surgical oncology: the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology 04/2012; 38(4):367-73. · 2.56 Impact Factor
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    ABSTRACT: In the present work the influence of a cross-section transition on freckle formation in the directionally solidified superalloy CMSX-4 was investigated. It was found that in stepwise expanding specimens the new freckle chains were not formed immediately at the bottom edge of the steps, but after an incubation height of about 10 mm. The flow behaviour through a mushy zone was simulated in a similar system, in which the cross section was changed stepwise. After an expansion of the cross section, the flow velocity slowed down significantly. This explained the suppression effect of the expanding step on the interdendritic convection and furthermore on the freckle formation. The step effect was successfully applied to avoid the freckle formation in expanding specimens by applying a step length smaller than the critical incubation length.
    IOP Conference Series Materials Science and Engineering 01/2012; 27(1).
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    ABSTRACT: The Central Asian Orogenic Belt (CAOB) is one of the largest accretionary collages in the world, and records a prolonged sequence of subduction‐accretion and collision processes. The Tarim Craton is located at the southernmost margin of the CAOB. In this study, the discovery of early Palaeozoic high‐pressure (HP) granulites from the Dunhuang block in the northeastern Tarim Craton is reported, and these rocks are characterized through detailed petrological and geochronological studies. The peak mineral assemblage of the HP mafic granulite is garnet + clinopyroxene + plagioclase + quartz + rutile, which is overprinted by amphibolite facies retrograde metamorphic assemblages. The calculated P–T conditions of the peak metamorphism are ∼1.4–1.7 GPa and ∼800 °C. The retrograde P–T conditions are ∼0.7 GPa and ∼700 °C. The metamorphic zircon grains from the HP mafic granulite show homogeneous CL‐images, low Th/U ratios and flat HREE patterns and yield a weighted mean 206Pb/238U age of 444 ± 5 Ma. The metamorphic zircon grains from the associated kyanite‐bearing garnet gneiss and garnet‐mica schist show a similar 206Pb/238U age of 429 ± 3 and 435 ± 4 Ma, respectively. The c. 440–430 Ma age is interpreted to mark the timing of HP granulite facies metamorphism in the Dunhuang block. The results from this study suggest that the Dunhuang block experienced continental subduction prior to the early Palaeozoic collisional orogeny between the northeastern Tarim Craton and the southern CAOB, and the Dunhuang area could be considered as the southward extension of the CAOB. It is suggested that the continental collision in the eastern part involving the Dunhuang block of the southern CAOB may have occurred c. 120 Ma earlier than in the western part involving the Tianshan orogen.
    Journal of Metamorphic Geology 01/2012; 30(8). · 3.40 Impact Factor

Publication Stats

395 Citations
146.91 Total Impact Points

Institutions

  • 2014
    • Shandong University
      • School of Medicine
      Chi-nan-shih, Shandong Sheng, China
  • 2013
    • Capital Medical University
      Peping, Beijing, China
    • Sichuan University
      Hua-yang, Sichuan, China
  • 2008–2013
    • Tsinghua University
      • • Center for Earth System Science
      • • Department of Precision Instruments and Mechanical Engineering
      • • Department of Environmental Engineering
      Peping, Beijing, China
  • 2012
    • Nanjing Normal University
      • College of Life Sciences
      Nan-ching, Jiangsu Sheng, China
    • Weill Cornell Medical College
      New York City, New York, United States
    • Peking University
      • State Key Laboratory of Nuclear Physics and Technology
      Beijing, Beijing Shi, China
    • Lakehead University Thunder Bay Campus
      • Department of Mechanical Engineering
      Thunder Bay, Ontario, Canada
    • Shanghai Jiao Tong University
      • Department of Nephrology (Renji)
      Shanghai, Shanghai Shi, China
  • 2011–2012
    • China University of Geosciences
      • Faculty of Earth Sciences
      Wu-han-shih, Hubei, China
    • Tongji Hospital
      Wu-han-shih, Hubei, China
  • 2010–2012
    • Beijing University of Posts and Telecommunications
      Peping, Beijing, China
    • Chang Gung University
      Hsin-chu-hsien, Taiwan, Taiwan
  • 2001–2012
    • Chinese Academy of Sciences
      • • State Key Laboratory of Polymer Physics and Chemistry
      • • Shenyang National Laboratory for Materials Science
      • • Institute of Metal Research
      Peping, Beijing, China
  • 2009
    • University of Jinan (Jinan, China)
      Chi-nan-shih, Shandong Sheng, China
  • 2007
    • Jiangsu University
      Chenkiang, Jiangsu Sheng, China
  • 2006–2007
    • Dalian University of Technology
      Lü-ta-shih, Liaoning, China
  • 2003
    • Northeast Institute of Geography and Agroecology
      • Shenyang National Laboratory for Materials Science
      Beijing, Beijing Shi, China
  • 1999
    • Beijing Medical University
      • Department of Dermatology
      Peping, Beijing, China
  • 1993–1995
    • Xi'an Jiaotong University
      Ch’ang-an, Shaanxi, China