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K B Jin,
H J Choi,
H T Kim,
E A Hwang,
S Y Han,
S B Park,
H C Kim,
E Y Ha, Y H Kim,
S I Suh,
K C Mun
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ABSTRACT: Long-term treatment with cyclosporine (CsA) results in chronic nephrotoxicity, which is known to be mediated by several cytokines including transforming growth factor-betal. Cytokines are known to play an important role in innate immunity, apoptosis, angiogenesis, cell growth, and differentiation. They are known to be involved in most disease processes, including cancer, cardiac disease, and nephrotoxicity. To evaluate changes of cytokines in a rat model of CsA-induced chronic nephrotoxicity, we performed a cytokine array.
Experiments were performed on two groups of rats; normal control group and CsA-treated group. Cytokine array in rat serum was performed using Cytokine Antibody Array I kit from RayBiotech.
Serum creatinine, urine creatinine, and creatinine clearance increased in the CsA-treated group. Among the several cytokines, the expressions of the lipopolysaccharide-induced CXC chemokine (LIX), monocyte chemoattractant protein 1 (MCP-1), nerve growth factor (beta-NGF), and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the CsA-treated group were increased above that of cytokines in the control group. The density of the LIX in controls was 0.62, and in the CsA-treated group was 1.24. The density of the MCP-1 in controls was 0.68, and in CsA-treated, 1.43. The density of the beta-NGF in controls was 0.62, and that in CsA-treated, 1.24. The density of the TIMP-1 in controls 1.13, and in CsA-treated, 1.40.
Our data suggested that among several cytokines elevated levels of the LIX, MCP-1, beta-NGF, and TIMP-1 are the contributing factors to CsA-induced nephropathy.
Transplantation Proceedings 11/2008; 40(8):2682-4. · 1.00 Impact Factor
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ABSTRACT: After organ transplantation, some patients suffer mild neurological symptoms such as tremor to severe complications including seizures and encephalopathy. Among the immunosuppressants, cyclosporine (CsA) can induce neurological side effects. However, the mechanisms of encephalopathy by CsA are not fully understood. We measured the production of reactive oxygen species (ROS) in the glioma cells after CsA treatment.
CsA (2.5 mmol/L) added to glioma cells was incubated for 60 minutes at 37 degrees C. ROS production was evaluated by measuring the fluorescent product from the oxidation of an oxidant-sensitive 2',7'-dichlorofluorescin using VICTOR3 multilabel counter.
CsA resulted in ROS production by glioma cells. The ROS production increased with the time of exposure to CsA.
These findings indicated that CsA may contribute to neurological side effects via ROS production.
Transplantation Proceedings 11/2008; 40(8):2742-3. · 1.00 Impact Factor
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ABSTRACT: A 51-year-old man was admitted with microscopic hematuria at 10 years after living donor renal transplantation. In order to distinguish between acute tubular necrosis and acute rejection, a graft biopsy was performed under ultrasound guidance at 1 month posttransplantation. Doppler sonography revealed 3 pulsatile cystic masses and an arteriovenous fistula (AVF) in the lower kidney pole. Selective transplant renal angiography revealed 3 pseudoaneurysms with an AVF supplied by a lobular artery in the lower pole. The diagnosis was AVF with pseudoaneurysm, which developed secondary to percutaneous renal allograft biopsy. Interventional treatment was performed because of the high risk for pseudoaneurysm rupture. The AVF and pseudoaneurysms were treated successfully by percutaneous transluminal embolization; renal function remained stable after embolization.
Transplantation Proceedings 10/2008; 40(7):2444-5. · 1.00 Impact Factor
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Transplantation Proceedings 03/2003; 35(1):126-7. · 1.00 Impact Factor
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Transplantation Proceedings 03/2003; 35(1):99-100. · 1.00 Impact Factor
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Transplantation Proceedings 12/2002; 34(7):2652-3. · 1.00 Impact Factor
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ABSTRACT: A new method is proposed for effective analysis of laminated plates incorporating accurate through-the-thickness distribution
of displacements, strains and stresses in the finite element formulation. It is a two-step analysis procedure. In the first
step, displacements are obtained using a post-processing procedure based on the three-dimensional stress equilibrium equations
and the thermoelasticity equations, from the results of FSDT finite element analysis. In the second step, the higher-order
through-the-thickness distribution of displacements are reflected on the subsequent finite element analysis. The effectiveness
of the present approach for the analysis of laminated plates is shown by numerical examples.
Computational Mechanics 01/2002; 29(3):226-242. · 2.07 Impact Factor
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Y S Lee,
S J Yoon,
T K Kwon, Y H Kim,
J H Woo,
M H Suh,
S I Suh,
W K Baek,
H J Kim,
S Y Ahn,
B K Choe,
J W Park
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ABSTRACT: There are many mutations in the gene encoding Hepatitis B virus (HBV) core antigen of chronic active hepatitis patients, and such mutations are most likely to be related to the severity of disease. Here, we constructed plasmids containing wild-type and deletion type of HBV core gene (HBc) to develop an experimental DNA vaccine and to compare immunogenicity of two types of HBc vaccine. Twenty-nine wild-types and seven deletion types of HBc were detected in sera of 32 Korean patients with chronic active hepatitis. Four wild-types (W1, W2, W4, W6) and two deletion types (D3, D4) of HBc were cloned into the pcDNA3 vector. Intramuscular immunization with wild-type HBc efficiently increased serum anti-HBc antibody response in a dose-dependent manner. Anti-HBc antibody response in mice injected with W6 increased 14 days after immunization, and peaked after 30 days and was maintained at least up to 50 days. W6 immunization induced a specific cytotoxic T lymphocyte response to W6-transfected 3LL (3LL-W6), and reduced the sizes of tumor mass of mice challenged with 3LL-W6 or 3LL transfected with D4. However, intramuscular immunization with D3 and D4 did not show antibody response at all. D3 and D4 have 157 bp (from 331 to 491 bp) and 122 bp (from 327 to 448 bp) gene deletion, respectively, and these encode class II MHC-restricted T-cell epitope. Altogether, these results suggest that mutant virus that has deleted HBc gene may evade immune systems due to loss of T-cell epitope.
Immunology Letters 09/2001; 78(1):13-20. · 2.53 Impact Factor
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Transplantation Proceedings 12/2000; 32(7):1777-8. · 1.00 Impact Factor
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ABSTRACT: Spectroscopic observations of outer planets, Titan, and comets from Earth-based and spaceborne observatories during the last three decades have produced a long list of molecules, atoms, radicals, ions, and dimers. The detections of these constituents are chronologically presented. We have reviewed spectroscopic studies on the auroral emissions and airglows of the giant planets, and briefly summarized the spectroscopic observations of dimers in the atmospheres of the giant planets and Titan. We also highlighted the recent detections of new molecular emissions and absorptions in the spectra of the giant planets, Titan, and recent bright comets from spaceborne or ground-based observatories. In order to present the limitations of current methods on the analyses of spectroscopic observations, we discussed issues on radiative transfer calculations including absorptions and emissions of molecules and particles in these atmospheres. We also reviewed current thoughts and theories on how to understand spectroscopically obtained molecular abundances and isotopic ratios in the atmospheres in terms of the origin and evolution of the solar system.
Planetary and Space Science.
