Wei Huang

Shandong University, Chi-nan-shih, Shandong Sheng, China

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Publications (2)2.14 Total impact

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    ABSTRACT: A mitochondrial gene cluster encoding cytochrome c oxidase subunit III (COX3), an ORF (called ORF250) similar to NADH dehydrogenase subunit VI (ND6), ten tRNA molecules, partial rRNA small subunit and rRNA large subunit from Trichoderma pseudokoningii S38 was cloned and sequenced. These genes are tandemly clustered on the mitochondrial genome of Trichoderma pseudokoningii S38. Phylogenetic analysis showed that cytochrome C oxidase subunits III exhibited high degree of similarity to sequences from Hypocrea jecorina, Verticillium lecanii, Podospora anserine, Neurospora crassa and Magnaporthe grisea (99, 90, 84, 82 and 79% identity, respectively). Prediction of transmembrane helices revealed that COX3 was a transmembrane protein. Northern dot blot analysis showed that the cytochrome c oxidase subunits III gene we had cloned is actively transcribed in the T. pseudokoningii mitochondria.
    DNA Sequence 11/2002; 13(5):271-5. DOI:10.1080/1042517021000019250 · 0.41 Impact Factor
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    ABSTRACT: A mitochondrial gene cluster, encoding proteins homologous to NADH dehydrogenase subunits II and III (ND2 and ND3) and seven tRNAs, from Trichoderma reesei QM9414 was cloned and sequenced. These genes are clustered tandemly on the mitochondrial genome of QM9414. Phylogenetic analysis showed that ND2 and ND3 were most closely related to the mitochondrial ND subunits II (71% identity) and III (70% identity) from Podospora anserine. Northern dot blot analysis showed that the nd2 and nd3 genes are actively transcribed in the T.reesei mitochondria.
    Biotechnology Letters 01/2002; 24(5):395-399. DOI:10.1023/A:1014529320482 · 1.74 Impact Factor