Yong Peng

Peking Union Medical College Hospital, Peping, Beijing, China

Are you Yong Peng?

Claim your profile

Publications (94)141.32 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The section Moutan of the genus Paeonia consists of eight species that are confined to a small area in China. A wide range of metabolites, including monoterpenoid glucosides, flavonoids, tannins, stilbenes, triterpenoids, steroids, paeonols, and phenols, have been found in the species belonging to section Moutan. However, although previous studies have analyzed the metabolites found in these species, the metabolic similarities that can be used for the chemotaxonomic distinction of section Moutan species are not yet clear. In this study, HPLC-DAD-based metabolic fingerprinting was applied to the classification of eight species: Paeoniasuffruticosa, Paeoniaqiui, Paeoniaostii, Paeoniarockii, Paeoniajishanensis, Paeoniadecomposita, Paeoniadelavayi, and Paeonialudlowii. In total, of the 47 peaks that exhibited an occurrence frequency of 75% in all 23 tree peony samples, 43 of these metabolites were identified according to their retention times and UV absorption spectra, together with combined HPLC-QTOF-MS. These data were compared with reference standard compounds. The 43 isolated compounds included 17 monoterpenoid glucosides, 11 galloyl glucoses, 5 flavonoids, 6 paeonols and 4 phenols. Principal component analysis (PCA), and hierarchical cluster analysis (HCA), showed a clear separation between the species based on metabolomics similarities and four groups were identified. The results exhibited good agreement with the classical classification based on the morphological characteristics and geographical distributions of the subsections Vaginatae F.C. Stern and Delavayanae F.C. Stern with the exception of P. decomposita, which was found to be a transition species between these two subsections. According to their metabolic fingerprinting characteristics, P. ostii and P. suffruticosa can be considered one species, and this result is consistent with the viewpoint of medicinal plant scientists but different from that of classical morphological processing. Significantly large variations were obtained in the metabolic profiles of P. delavayi, whereas no significant difference was found between P. delavayi and P. ludlowii. This indicates that these two species have a close genetic relationship. In conclusion, the combination of HPLC-DAD and multivariate analyses has great potential for guiding future chemotaxonomic studies to examine the potential pharmaceutical value of the effective constituents of tree peony species and appears to be able to clarify the confusion and skepticism associated with the reported morphology- and molecular phylogenetics-based taxonomy of tree peonies.
    Phytochemistry 09/2014; · 3.35 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Traditional macroscopic and microscopic identification methods of medicinal materials are economical and practical, but usually experience-based due to few chemical supports. Here histochemical evaluation on bioactive components of Coptidis Rhizoma (CR) in anatomic sections using laser microdissection and liquid chromatography-mass spectrometry (LMD-LC-MS) was developed to correlate the inner quality and outer features of materials from different growing areas. Results of a total 33 peaks representing potential different alkaloids were detected and 8 common peaks were identified as the major alkaloids, namely magnoflorine, thalifendine, columbamine, epiberberine, jatrorrhizine, coptisine, palmatine, and berberine. Six major alkaloids were quantified in the top and middle sections of raw materials and in their tissues and cells at the same time. Histochemical analyses showed consistent results with direct determination in raw materials and explained the reason why top sections of all samples contained higher contents of alkaloids by giving out attributions of each alkaloid in different anatomic sections. Besides, results manifested the distribution and accumulation rules of alkaloids in diverse tissues and cells of CR. This study demonstrates an effective and scientific way to correlate bioactive components and morphological features of medicinal materials, which is beneficial to future research, agriculture and application. Copyright © 2014 John Wiley & Sons, Ltd.
    Drug Testing and Analysis 09/2014; · 3.17 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: One hundred and forty-four samples of Chishao and Baishao, which represented six species of Paeonia L. were evaluated for their genetic variation, genetic differentiation and phylogenetic relationship, based on the nuclear ribosomal DNA internal transcribed spacer (ITS) region. Samples from representative of the population were then used to do a cultivation comparison experiment, and then to identify the contents of the active ingredients. The results showed there were differences in the haplotype distribution and frequency between populations of Chishao and Baishao. An analysis of molecular variance (AMOVA) indicated statistically significant (P<0.001) genetic differentiation between the populations of wild and cultivated Paeonia lactiflora Pall. The albiflorin content between Chishao and Baishao was also significant different (P<0.05). All the results clearly illustrate that currently cultivated P. lactiflora cannot be used as a substitute for Chishao.
    Biological & Pharmaceutical Bulletin 07/2014; · 1.85 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Single copy genes are common across angiosperm genomes. With the sufficiently high quality sequenced genomes, the identification of large-scale single copy genes among multiple species is possible. Although some characteristics have been reported, our study provides novel insights into single copy genes.
    BMC genomics. 06/2014; 15(1):504.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Our goal is to test if CS1 could be targeted by CAR T cells to treat MM. We generated a retroviral construct of a CS1-specific CAR and engineered primary human T cells expressing the CAR. We then tested the capacity of CS1-CAR T cells to eradicate human multiple myeloma tumor cells in vitro, ex vivo and in vivo using orthotopic MM xenograft mouse models. In vitro, compared to mock-transduced T cells, upon recognizing CS1 positive MM cells, CS1-CAR-tranduced T cells secreted more IFN-γ as well as IL-2, expressed higher levels of the activation marker CD69, showed higher capacity for degranulation, and displayed enhanced cytotoxicity. Ectopically forced expression of CS1 in MM cells with low CS1 expression enhanced recognition and killing by CAR T cells. Ex vivo, CS1-CAR T cells also showed similarly enhanced activities when responding to primary MM cells. More importantly, in orthotopic MM xenograft mouse models, adoptive transfer of human primary T cells expressing CS1-CAR efficiently suppressed the growth of human MM.1S and IM9 myeloma cells and significantly prolonged mouse survival. CS1 is a promising antigen that can be targeted by CAR-expressing T cells for treatment of MM.
    Clinical Cancer Research 03/2014; · 7.84 Impact Factor
  • Chemistry & Biodiversity 03/2014; 11(1):55-72. · 1.81 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The mechanism by which the 8q24 MYC enhancer region, including cancer-associated variant rs6983267, increases cancer risk is unknown due to the lack of protein-coding genes at 8q24.21. Here we report the identification of long noncoding RNAs named cancer-associated region long noncoding RNAs (CARLos) in the 8q24 region. The expression of one of the long noncoding RNAs, CARLo-5, is significantly correlated with the rs6983267 allele associated with increased cancer susceptibility. We also found the MYC enhancer region physically interacts with the active regulatory region of the CARLo-5 promoter, suggesting long-range interaction of MYC enhancer with the CARLo-5 promoter regulates CARLo-5 expression. Finally, we demonstrate that CARLo-5 has a function in cell-cycle regulation and tumor development. Overall, our data provide a key of the mystery of the 8q24 gene desert.
    Proceedings of the National Academy of Sciences 03/2014; · 9.81 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The tribe Chelidonieae comprises 23 species of eight genera with an extensive distribution and a long medicinal usage history both in China and Western countries. A large number of chemical constituents have been isolated and identified from the species in tribe Chelidonieae, such as alkaloids, organic acids, and their derivatives, aromatics, triterpenoids, sterols, essential oils, and proteins, most of which possess a variety of bioactivities, especially for the antibacterial, anti-inflammation, antitumor, analgesia, anti-oxidation, and antiparasitic activity. Meanwhile, potential toxicities have been discovered in some constituents. Therefore, the species in tribe Chelidonieae have become a rich source for new drug discovery, biologic study, and mechanism research. This paper presents comprehensive information of the chemical constituents, pharmacological and toxicological research on the plants in tribe Chelidoieae, which is a reference for the plants in this tribe for further development.
    Chinese Herbal Medicines. 02/2014; 6(1):1–21.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone glycosides are a series of major active ingredients found in all three species. They are key intermediates in the anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic. A simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array (UPLC-PDA) detector was developed for the simultaneous determination of six anthraquinone glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-O-glucoside, emodin-8-O-glucoside. Nine batches of R. tanguticum Maxim, five batches of Rheum officinale Baill and thirteen batches of R. palmatum L. were submitted to the multi-component analysis. The results showed that the anthraquinone glycoside content varied significantly even within the same species. The data of the anthraquinone glycoside content in rhubarb were assessed by correlational analysis, principal component analysis, spatial analysis, vertical slice analysis through SPSS and Geographic Information System (ArcGis) to determine the correlations of the anthraquinone glycoside content with plant species, geographical distribution and altitude. The results showed that the anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the anthraquinone glycoside content and the geographic distribution of the rhubarb. Through correlational analysis, altitude was found to be the main factor that affects the anthraquinone glycoside content in rhubarb. Rhubarb grown at higher altitude has higher anthraquinone glycoside content. This work provides a rapid, sensitive and accurate UPLC-PDA method for the simultaneous determination of six anthraquinone glycosides in rhubarb. The anthraquinone glycoside content varied significantly within the same species. The relationship of the anthraquinone glycoside content with plant species, geographic distribution and altitude were studied using correlational analysis, principal component analysis and spatial autocorrelation analysis through SPSS and ArcGIS. Plant species and geographic distribution were found not to affect the content of the six anthraquinone glycosides in rhubarb. The variations in the anthraquinone glycoside content were primarily due to the different altitude where the plant was grown.
    Chemistry Central Journal 10/2013; 7(1):170. · 1.31 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: MicroRNAs (miRNAs) are small 19- to 24-nt noncoding RNAs that have the capacity to regulate fundamental biological processes essential for cancer initiation and progression. In cancer, miRNAs may function as oncogenes or tumor suppressors. Here, we conducted global profiling for miRNAs in a cohort of stage 1 nonsmall cell lung cancers (n = 81) and determined that miR-486 was the most down-regulated miRNA in tumors compared with adjacent uninvolved lung tissues, suggesting that miR-486 loss may be important in lung cancer development. We report that miR-486 directly targets components of insulin growth factor (IGF) signaling including insulin-like growth factor 1 (IGF1), IGF1 receptor (IGF1R), and phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1, or p85a) and functions as a potent tumor suppressor of lung cancer both in vitro and in vivo. Our findings support the role for miR-486 loss in lung cancer and suggest a potential biological link to p53.
    Proceedings of the National Academy of Sciences 08/2013; · 9.81 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The genus Fritillaria is a botanical source for various pharmaceutically active components, which have been commonly used in traditional Chinese medicine for thousands of years. Increasing interest in Fritillaria medicinal resources has led to additional discoveries of steroidal alkaloids, saponins, terpenoids, glycosides and many other compounds in various Fritillaria species, and to investigations on their chemotaxonomy, molecular phylogeny and pharmacology. In continuation of studies on Fritillaria pharmacophylogeny, the phytochemistry, chemotaxonomy, molecular biology and phylogeny of Fritillaria and their relevance to drug efficacy is reviewed. Literature searching is used to characterize the global scientific effort in the flexible technologies being applied. The interrelationship within Chinese Bei Mu species and between Chinese species, and species distributed outside of China, is clarified by the molecular phylogenetic inferences based on nuclear and chloroplast DNA sequences. The incongruence between chemotaxonomy and molecular phylogeny is revealed and discussed. It is essential to study more species for both the sustainable utilization of Fritillaria medicinal resources and for finding novel compounds with potential clinical utility. Systems biology and omics technologies will play an increasingly important role in future pharmaceutical research involving the bioactive compounds of Fritillaria.
    Chinese journal of natural medicines. 07/2013; 11(4):330-344.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A rapid, sensitive, and accurate HPLC-DAD method was developed and validated for simultaneous determination of one phenolic glycoside and seven monoterpene glycosides, including 1-O-β-d-(4-hydroxybenzoyl)glucose (1), pyridylpaeoniflorin (2), (8R)-piperitone-4-en-9-O-β-d-glucopyranoside (3), oxypaeoniflorin (4), 6'-O-β-glucopyranosylalbiflorin (5), albiflorin (6), β-gentiobiosylpaeoniflorin (7), and paeoniflorin (8), in 44 batches of peony seeds from nine Paeonia species collected from different areas. Using the optimised method, separations were conducted with a YMC-pack ODS-A column with water/formic acid and methanol as the mobile phase. All eight analytes demonstrated good linearity (r(2)>0.9993). The recoveries, measured at three concentration levels, varied from 98.20% to 103.81%. Six compounds including 1 and 4-8 occur ubiquitously in all the seeds of nine Paeonia species, and compounds 2 and 3 showed undetectable levels or very low content in several samples. The seed samples were classified into several groups, which coincide with the taxonomy of Paeonia at the section level. Peony seed might be a useful resource in developing new herbal or food products.
    Food Chemistry 06/2013; 138(4):2108-14. · 3.33 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To develope a method of RSLC fingerprint analysis for the quality evaluation of Lycii Cortex, and assess the differences of Lycii Cortex from Lycium chinense and L. barbarum. All separations were performed using a Rapid Separation Liquid Chromatography (RSLC) system with an Acquity UPLC C18 (2.1 mm x 100 mm, 1.7 microm) column. The mobile phase was consisted of acetonitrile and 0.1% aqueous acetic acid. The flow rate was 0.3 mL/min using gradient elution. The column temperature was 40 degrees C and detection wavelength was set at 315 nm. Lycii Cortex samples collected from different habitats were assessed by similarity analysis (SA), hierarchical clustering analysis (HCA) and principle component analysis (PCA). The characteristic RSLC fingerprint was established with 14 common peaks. 14 hatches of samples were classified as 2 clusters by HCA and PCA, and the result demonstrated that there were differences in the contents of chemical composition between 2 species of Lycii Cortex. The established RSLC fingerprint is specific and the method is rapid, simple and reliable, which can be used to control the quality of Lycii Cortex in the markets.
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 06/2013; 36(6):895-9.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cimicifugeae is one of the rich sources for various active components and the health promoting and therapeutic values of the components have been corroborated by long-term use in folk medicine and traditional Chinese medicine. Increasing interest in Cimicifugeae pharmaceutical resources has led to the further discoveries of triterpenoid saponins, phenolic compounds, chromones, and many other compounds in various species of Cimicifugeae, and to the investigations on their chemotaxonomy, molecular phylogeny, and bioactivities. Based on our pharmacophylogenetic studies, the progress in phytochemistry, chemotaxonomy, molecular biology, and phylogeny of Cimicifugeae had been summarized since 2007, especially Cimicifuga L. ex Wernisch. and Actaea L., and their relevance to therapeutic efficacy. An exhaustive literature survey is used to characterize the global scientific effort in the phytochemical and biological studies of Cimicifugeae. More triterpenoid saponins have been found in various species, among which the cimigenol type (type A) is predominant. The versatile bioactivities of saponins and extracts, as well as those of phenolics and other ingredients, were summarized and discussed. The morphology-based five-genus classification of Cimicifugeae is not supported by molecular phylogeny. Molecular phylogeny based on nuclear and chloroplast DNA sequences tends to merge Cimicifuga Wernisch., Souliea Franch., and Actaea L. into a single genus. It is indispensable to integrate the emerging technologies into Cimicifugeae research for both the sustainable utilization of Cimicifugeae pharmaceutical resources and finding novel compounds with potential clinical utility and less adverse effects. Systems biology and omics technologies would play an increasingly important role in booming pharmaceutical research involving bioactive compounds of Cimicifugeae.
    Chinese Herbal Medicines. 05/2013; 5(2):81–95.
  • [Show abstract] [Hide abstract]
    ABSTRACT: MicroRNAs (miRNAs) bind to complementary sequences of target mRNAs, resulting in translational repression or target degradation and thus gene silencing. MiRNAs are abundantly found in circulating blood, yet whether, as a class of regulatory molecules, they may interact with human natural killer (NK) cells has not been explored. Here we found that the treatment of human NK cells with several mature miRNAs, in the presence of a low concentration of IL-12, induced CD69 expression, IFN-γ production, and degranulation marker CD107a expression. In vivo, infusion of several miRNAs alone in murine peripheral blood also resulted in comparable NK but not T cell activation. Furthermore, miRNA administration significantly protected mice from tumor development in an NK cell-dependent manner. Mechanistically, we found that miRNA stimulation led to downstream activation of NF-κB, an effect that was blunted by a block in Toll-like receptor (TLR) 1 signaling and was attenuated in lymphoma patients. Knockdown of TLR1 resulted in less activation by miRNAs. Collectively, we show that miRNAs have a capacity to selectively activate innate immune effector cells that is at least in part via the TLR1-NF-κB signaling pathway. This may be important in the normal host defense against infection and/or malignant transformation.
    Blood 04/2013; · 9.78 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In China, Kudingcha has been used for almost 2,000 years as a tea to quench thirst, remove phlegm, refresh the mind, and improve eyesight. The group of large-leaved Kudingcha is coveted for its potential effects on lipid metabolism, which are attributed to the presence of characteristic ingredients. This contribution reviews studies from the past few decades regarding the plant characteristics, ethnobotanical usages, chemical constituents, and related biological activities of the large-leaved Kudingcha (Ilex latifolia Thunb and Ilex kudingcha C.J. Tseng). Triterpenoids, phenolic acids, flavonoids, and essential oils are the main metabolites in the large-leaved Kudingcha, and these ingredients protect the vascular system, regulate lipid metabolism, and have antioxidant, hypoglycemic, and anti-tumor effects. Moreover, large-leaved Kudingcha shares several properties with the popular green tea and the Yerba maté from South America.
    Journal of Natural Medicines 03/2013; · 1.52 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Ilex is a botanical source for various health-promoting and pharmaceutically active compounds that have been used in traditional Chinese medicine and food for thousands of years. Increasing interest in Ilex pharmaceutical and food resources has led to additional discoveries of terpenoids, saponins, polyphenols (especially flavonoids), glycosides, and many other compounds in various Ilex species, and to investigation of their chemotaxonomy, molecular phylogeny and pharmacology. In continuation of our studies on Ilex pharmacology and phylogeny, we review the phytochemistry, chemotaxonomy, molecular biology and phylogeny of Ilex species and their relevance to health-promotion and therapeutic efficacy. The similarity and dissimilarity between Ilex paraguariensis, the source plant of mate tea, and the source plants of large-leaved Kudingcha (e.g., Ilex kudingcha and Ilex latifolia) are discussed. It is essential to utilize emerging technologies in non-Camellia tea studies to promote the sustainable utilization of Ilex resources and the identification and development of novel compounds with potential health and clinical utility. Systems biology and “-omics” technologies will play an increasingly important role in pharmaceutical and food research on the bioactive compounds of Ilex species.
    Acta Pharmaceutica Sinica B. 02/2013; 3(1):8–19.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A rapid and accurate ultra-performance liquid chromatography-photo-diode array (UPLC-PDA) detection method was established for simultaneous determination of 5 compounds including polydatin, resveratrol, emodin-8-glucoside, emodin, and physcion in Polygonum cuspidatum. Twenty-six batches of samples were collected from different areas and markets in China. The compounds were separated in less than 12 min using a C18 column with acetonitrile and water containing 0.5% acetic acid as the mobile phase at a flow rate of 0.3 mL/min. The precision (intra-day and inter-day assay), stability, limits of detection and quantitation, linearity, and recovery were evaluated for this method. RSD for intra-day precision, inter-day precision, and stability were less than 2.5%, 3.5%, and 2.19%, respectively. Recovery ranged from 96.78% to 104.58% (RSD Keywords: POLYGONUM CUSPIDATUM; constituent determination; hierarchical cluster analysis; principal component analysis; ultra performance liquid chromatography Document Type: Research Article DOI: http://dx.doi.org/10.1080/10826076.2012.723096 Affiliations: 1: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing, P. R. China 2: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing, P. R. China 3: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing, P. R. China Publication date: December 14, 2013 $(document).ready(function() { var shortdescription = $(".originaldescription").text().replace(/\\&/g, '&').replace(/\\, '<').replace(/\\>/g, '>').replace(/\\t/g, ' ').replace(/\\n/g, ''); if (shortdescription.length > 350){ shortdescription = "" + shortdescription.substring(0,250) + "... more"; } $(".descriptionitem").prepend(shortdescription); $(".shortdescription a").click(function() { $(".shortdescription").hide(); $(".originaldescription").slideDown(); return false; }); }); Related content In this: publication By this: publisher By this author: Ma, Pei ; Luo, Kun ; Peng, Yong ; Wang, Wanying ; Zhang, Xinyao ; Liu, Yanze ; Zhao, Xiaoling ; Xu, Lijia ; Xiao, Peigen GA_googleFillSlot("Horizontal_banner_bottom");
    Journal of Liquid Chromatography &amp Related Technologies 01/2013; 36(20). · 0.57 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Bioactive components from dietary supplements such as curcumin may represent attractive agents for cancer prevention or treatment. DNA methylation plays a critical role in acute myeloid leukemia (AML) development, and presents an excellent target for treatment of this disease. However, it remains largely unknown how curcumin, a component of the popular Indian spice turmeric, plays a role in DNA hypomethylation to reactivate silenced tumor suppressor genes and to present a potential treatment option for AML. Here we show that curcumin down-regulates DNMT1 expression in AML cell lines, both and , and in primary AML cells . Mechanistically, curcumin reduced the expression of positive regulators of DNMT1, p65 and Sp1, which correlated with a reduction in binding of these transcription factors to the DNMT1 promoter in AML cell lines. This curcumin-mediated down-regulation of DNMT1 expression was concomitant with tumor suppressor gene reactivation, hypomethylation of the promoter, G1 cell cycle arrest, and induction of tumor cell apoptosis in vitro. In mice implanted with the human AML MV4-11 cell line, administration of curcumin resulted in remarkable suppression of AML tumor growth. Collectively, our data indicate that curcumin shows promise as a potential treatment for AML, and our findings provide a basis for future studies to test the clinical efficacy of curcumin - whether used as a single agent or as an adjuvant - for AML treatment.
    PLoS ONE 01/2013; 8(2):e55934. · 3.53 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A rapid, sensitive, and accurate HPLC-DAD method was developed and validated for simultaneous determination of 10 stilbenes, which include suffruticosol A, suffruticosol B, suffruticosol C, resveratrol (E)-form, cis--viniferin, trans--viniferin, cis-suffruticosol D, cis-gnetin H, trans-suffruticosol D and gnetin H, in 44 plant samples from 9 different PAEONIA species. In this optimized method, separations were carried out on an YMC-pack ODS-A column with water and methanol as mobile phase. The flow rate was set at 1.0 mL/min and the detector wavelength was set at 230 nm. The results showed that all the 10 analytes showed good linearity (r2 > 0.9994). The recoveries, measured at three concentration levels, varied from 97.19% to 102.89%. This method was also validated with respect to precision, repeatability, stability, and successfully applied to simultaneous determination of 10 stilbenes in 44 batches of peony seeds from 9 PAEONIA species collected from different areas.
    Journal of Liquid Chromatography &amp Related Technologies 01/2013; 36(12). · 0.57 Impact Factor

Publication Stats

259 Citations
141.32 Total Impact Points

Institutions

  • 2007–2014
    • Peking Union Medical College Hospital
      Peping, Beijing, China
  • 2013
    • Dalian Jiaotong University
      Lü-ta-shih, Liaoning, China
  • 2006–2013
    • Chinese Academy of Medical Sciences
      Peping, Beijing, China
  • 2012
    • The Ohio State University
      • Department of Molecular Virology, Immunology and Medical Genetics
      Columbus, OH, United States
    • Beijing Technology and Business University
      Peping, Beijing, China
  • 2004–2012
    • Hong Kong Baptist University
      • • Centre for Cancer and Inflammation Research (CCIR)
      • • School of Chinese Medicine
      Kowloon, Hong Kong
  • 2011
    • Institute of Medicinal Plant Development
      Peping, Beijing, China
  • 2009
    • Nanjing University of Traditional Chinese Medicine
      • Department of Chinese Pharmacy
      Peping, Beijing, China
  • 2000
    • Beijing University of Chinese Medicine and Pharmacology
      Peping, Beijing, China