W Yu

Rochester General Hospital, Rochester, New York, United States

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Publications (2)2.62 Total impact

  • W Yu · J O Naim · R J Lanzafame ·
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    ABSTRACT: Low-level laser irradiation at certain fluences and wavelengths can enhance the release of growth factors from fibroblasts and stimulate cell proliferation in vitro. We evaluated whether low-level laser irradiation can improve wound healing in diabetes mellitus. Genetically diabetic mice (C57BL/Ksj/db/db) were used as the animal model for this wound healing study. The experimental animals were divided among four groups: negative control, positive control (topical basic fibroblast growth factor [bFGF] on wound), laser therapy group; and a combination group of laser therapy and topical bFGF. An argon dye laser (Lexel Auora Model 600) at a wavelength of 630 nm and an output of 20 m W/cm2 was used as the light source. The speed of wound closure and histological evaluation were used to analyze the experimental results. Laser irradiation enhanced the percentage of wound closure over time as compared to the negative control group (58.4 +/- 2.6 vs. 40.8 +/- 3.4 at day 10 and 95.7 +/- 2 vs. 82.3 +/- 3.6 at day 20, P < .01). Histological evaluation showed that laser irradiation improved wound epithelialization, cellular content, granulation tissue formation, and collagen deposition in laser-treated wounds as compared to the negative control group (6.4 +/- 0.16 vs. 3.8 +/- 0.13 at day 10 and 12 +/- 0.21 vs. 8.2 +/- 0.31, P < .01). This study of laser biostimulation on wound healing in diabetic mice suggests that such therapy may be of great benefit in the treatment of chronic wounds that occur as a complication of diabetes mellitus.
    Lasers in Surgery and Medicine 01/1997; 20(1):56-63. · 2.62 Impact Factor
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    ABSTRACT: This study was undertaken to determine if methotrexate (MTX) is effective against tumor cells surviving photodynamic therapy (PDT). C6 rat glioma cells were exposed to Photofrin and irradiated at 630 nm using power densities of 1.2 or 4.8 J/cm2 to simulate the conditions for cells in solid tumors which survive PDT. Cells were then treated with MTX at 5.0, 0.5, or 0.05 microM concentrations. MTT assay of cell proliferation was performed at 24, 48, 72, and 96 h postirradiation. During the first 48 h of incubation, MTX alone was more effective than PDT and MTX. After 48 h, the combination treatment was more effective. Further studies of combined PDT and chemotherapy are warranted.
    Journal of Clinical Laser Medicine & Surgery 05/1996; 14(2):55-8.

Publication Stats

194 Citations
2.62 Total Impact Points


  • 1997
    • Rochester General Hospital
      Rochester, New York, United States