W Zhao

Publications (10)28.64 Total impact

• Article: Segmentation expression of capsid protein as an antigen for the detection of avian nephritis virus infection in chicken flocks.

  W Zhao, A L Zhu, Y Yu, C L Yuan, C X Zhu, D L Lan, Z B Yang, L Cui, X G Hua
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  ABSTRACT: Subclinical pathological changes in the kidneys of broiler chickens and suppression of growth caused by the avian nephritis virus (ANV) affect poultry flocks worldwide. A test for detection of virus-specific antibodies in serum would be useful for epidemiological investigations, however the poor propagation in cell cultures has restricted the development of serological tests based on the use of ANV particles as antigens. An enzyme-linked immunosorbent assay (ELISA) was developed for detection of ANV-specific antibodies in chicken serum, using a recombinant protein antigen prepared by segmentation expression of the capsid protein antigen epitope of ANV (HM029238) transfected into Escherichia coli. The expressed fusion protein was detected by Western blotting with ANV-positive serum, and the optimal immunoreactive fusion P1 protein was determined. Using the optimized P1-ELISA, ANV-specific antibodies were detected in commercial chicken flocks aged 10-25 days obtained from the Liaoning Province, China. Out of 960 serum samples, 459 (47.8%) were positive for infection with ANV. These results indicate that the P1-ELISA is helpful for preliminary serological diagnosis of ANV infection, and could be used to for screening in ANV infection and for determining antibodies against ANV.
  Journal of virological methods 01/2012; 179(1):57-61. · 2.13 Impact Factor
• Article: Detection of astrovirus infection in pigeons (Columbia livia) during an outbreak of diarrhoea.

  W Zhao, A L Zhu, C L Yuan, Y Yu, C X Zhu, D L Lan, Z B Yang, L Cui, X G Hua
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  ABSTRACT: Avian astrovirus infections are widespread in many countries, and infections have been linked to enteritis and increased mortality in young poultry. Although pigeons are treated as an important poultry product in some countries, their diseases are often poorly understood and astrovirus infection in pigeons has not been reported. In the present study, faecal samples were collected during an outbreak of gastrointestinal illness in a population of Shanghai pigeons. The samples were examined for astroviruses by reverse transcription-polymerase chain reaction. Eighty-nine per cent (40/45) and 4% (2/45) were found to be positive for avian nephritis virus (ANV) and chicken astrovirus, respectively. One positive sample indicated a co-infection with both ANV and chicken astrovirus. Phylogenetic analysis based on the partial polymerase gene sequence and full-length capsid protein from published avian astrovirus sequences in GenBank revealed that the pigeon viruses detected in this study were evolutionarily closely related to chicken ANV. The present study provided evidence for the presence of astrovirus in pigeons and suggests that cross-infection between pigeons and commercial chickens was likely. Whether the astroviruses in pigeons were responsible for the diarrhoea remains to be determined.
  Avian Pathology 08/2011; 40(4):361-5. · 1.71 Impact Factor
• Article: Complete sequence and genetic characterization of pigeon avian nephritis virus, a member of the family Astroviridae.

  W Zhao, A L Zhu, Y Yu, C L Yuan, C X Zhu, Z B Yang, L Cui, X G Hua
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  ABSTRACT: In the current study, the complete genome sequence of a member of the family Astroviridae isolated from pigeons was determined through genetic characterization and phylogeny analysis. The isolated genome sequence was proposed to be that of pigeon avian nephritis virus (ANV), whose genome structure and characteristics were similar to previously reported avian astroviruses. The sequenced ssRNA genome comprises 6928 nucleotides, excluding the poly(A) tail, and contains three open reading frames. Phylogenetic analysis using a partial nucleotide sequence of the polymerase gene and the entire amino acid sequence of the full-length capsid protein revealed that pigeon avian nephritis virus is closely related to the previously published ANV, especially to the Japanese G-4260 and Chinese strains. This investigation provides information on the sequence and genetic characteristics of this virus and contributes to a better understanding of pigeon ANV and the possible occurrence of astrovirus transmission between chickens and pigeons.
  Archives of Virology 05/2011; 156(9):1559-65. · 2.11 Impact Factor
• Article: Isolation and characterization of canine astrovirus in China.

  A L Zhu, W Zhao, H Yin, T L Shan, C X Zhu, X Yang, X G Hua, L Cui
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  ABSTRACT: In this study, we first investigated the prevalence of astrovirus in stools of dogs with and without diarrhea in Shanghai, China. Of all the specimens, 22 (12.02%) from the 183 dogs with diarrhea and none (0%) from the 138 healthy controls were positive for astrovirus. Furthermore, we cloned partial sequences of ORF1b (442 bp) and the entire sequences of ORF2 (2475 bp). Phylogenetic analysis showed that the new isolates were belonged to genus Mamastrovirus and most closely clustered with the Italy strain, based on the ORF2 sequences available. However, the new isolates and the Italy strain were divided into two different clusters. The new isolates may be a new strain of canine astrovirus.
  Archives of Virology 05/2011; 156(9):1671-5. · 2.11 Impact Factor
• Article: Sequence analyses of the representative Chinese-prevalent strain of avian nephritis virus in healthy chicken flocks.

  W Zhao, X G Hua, L Yuan, L Cui, T L Shan, X Q Dai, A L Zhu, Y Yu, C X Zhu, Z B Yang
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  ABSTRACT: Avian nephritis virus (ANV), which belongs to the Astroviridae family, has been associated with acute nephritis in chickens. Cases of ANV infection have been recorded in Japan and in several European countries. However, related studies have never been performed in China. Thus, this study isolated ANV in Chinese chicken flocks. ANV RNA was detected by reverse transcription-PCR in stool samples collected from healthy layer chickens in the Sichuan Province of China in 2009. Of the 192 stool specimens collected, 32.3% (62/192) were positive for ANV infection. The whole genome of ANV-Sichuan54, the first representative Chinese strain, was 6941 nucleotides in length, including the 5' untranslated region, three open reading frames (ORFs), a 3' UTR, and a poly-(A) tail. Comparative and phylogenetic analyses based on partial RNA-dependent RNA polymerase (ORF1b) demonstrated that the majority of ANV investigations were more closely related to the U.S. ANV strain (DQ324827-324836) than to the G-4260 (AB033998).
  Avian Diseases 03/2011; 55(1):65-9. · 1.46 Impact Factor
• Article: Molecular detection of Saffold Virus in children in Shanghai, China.

  X Q Dai, C L Yuan, Y Yu, W Zhao, Z B Yang, L Cui, X G Hua
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 02/2011; 50(2):186-7. · 3.12 Impact Factor
• Article: Molecular detection and sequence analysis of feline Torque teno virus (TTV) in China.

  C X Zhu, T L Shan, L Cui, X N Luo, Z J Liu, S D Tang, Z W Liu, C L Yuan, D L Lan, W Zhao, X G Hua
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  ABSTRACT: In the present study, two isolates (SH-F1 and SH-F2) of Torque teno felis virus (feline TTV) were detected in 16 (12.5%) serum samples collected from cats in China. Their full length genomes were cloned and sequenced. The results showed that they were 2063bp in length and contained three open reading frames (ORFs) (ORF1: nt438-1748, ORF2: nt268-585 and ORF3: nt268-581, 1461-1842). Phylogenetic analysis showed that they were clustered with the strain of Japan (Fc-TTV4, AB076003) and the strain of France (PRA4, EF538878). Sequence analysis indicated that SH-F1 had high (97.5% and 93.3%) identity with the strain of Japan and the strain of France, and SH-F2 shared 94.5% and 92.1% homology with them, respectively. In conclusion, we demonstrated that feline TTV is present in China.
  Virus Research 12/2010; 156(1-2):13-6. · 2.94 Impact Factor
• Article: Porcine torque teno virus infections in China.

  C X Zhu, L Cui, T L Shan, X N Luo, Z J Liu, C L Yuan, D L Lan, W Zhao, Z W Liu, X G Hua
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 08/2010; 48(4):296-8. · 3.12 Impact Factor
• Source

  Available from: Tongling Shan

  Article: Human parechovirus infections in monkeys with diarrhea, China.

  T L Shan, C M Wang, L Cui, Eric Delwart, C L Yuan, W Zhao, W Guo, X Q Dai, Y Yu, X G Hua
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  ABSTRACT: Information about human parechovirus (HPeV) infection in animals is scant. Using 5' untranslated region reverse transcription-PCR, we detected HPeV in feces of monkeys with diarrhea and sequenced the complete genome of 1 isolate (SH6). Monkeys may serve as reservoirs for zoonotic HPeV transmissions and as models for studies of HPeV pathogenesis.
  Emerging Infectious Diseases 07/2010; 16(7):1168-9. · 6.79 Impact Factor
• Article: Human cosavirus infections in children in China.

  X Q Dai, X G Hua, T L Shan, Eric Delwart, W Zhao
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 07/2010; 48(3):228-9. · 3.12 Impact Factor