Wei Gao

Publications (2)7.29 Total impact

• Article: Molecular characterization of group G Streptococcus dysgalactiae subsp. equisimilis recovered from patients and healthy people in China.

  Jun Yin, Sangjie Yu, Xiaorong Liu, Yan Li, Wei Gao, Lin Zhou, Lili Ji, Xuzhuang Shen, Yonghong Yang
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  ABSTRACT: Beta-Hemolytic group G streptococci cause a considerable invasive disease burden and sometimes disease outbreaks. Little is known about the critical epidemiologic parameter of genetic relatedness between isolates. We determined the emm types of 65 Streptococcus dysgalactiae subsp. equisimilis isolates. We formulated multilocus sequence typing (MLST) primers with 6 of the 7 loci corresponding to the Streptococcus pyogenes MLST scheme. We performed MLST with 69 S. dysgalactiae subsp. equisimilis isolates to represent each emm type identified. These strains were further analyzed by pulsed-field gel electrophoresis (PFGE) typing. Sixteen emm types were observed. Eighteen unique combinations of allelic profiles (sequence types [STs]) were obtained with 12 profiles each accounting for multiple isolates. Forty-one MLST STs were observed. Analysis of the PFGE patterns generated revealed 10 clones. Over 80% of the isolates were distributed in 3 large clones. Isolates within 16 redundantly represented S. dysgalactiae subsp. equisimilis emm types shared identical or nearly identical STs and subtypes of PFGE, demonstrating concordance between the emm type and genetic relatedness. It is conceivable that some particular characteristics in the genomes of these strains are responsible for their predominance in different regions.
  Diagnostic microbiology and infectious disease 11/2011; 72(1):41-6. · 2.45 Impact Factor
• Article: Prevalence and mechanism of resistance to antimicrobial agents in group G streptococcal isolates from China.

  Jun Yin, Sangjie Yu, Xiaorong Liu, Ye Li, Wei Gao, Xiang Ma, Yonghong Yang
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  ABSTRACT: Eighty group G streptococcal stains were collected from Chinese children. Susceptibility testing was done by a double-dilution and a disk diffusion method. PCR was used to test drug-resistant genes, and the χ(2) test and definite probability methods were used to test for statistically significant differences among the three groups. Thirty-four isolates (42.5%) showed resistance to erythromycin. There are differences between the resistance characteristics of group G streptococci from different regions of China.
  Antimicrobial Agents and Chemotherapy 10/2010; 55(1):402-4. · 4.84 Impact Factor