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Yuri A Gorby,
Svetlana Yanina,
Jeffrey S McLean,
Kevin M Rosso,
Dianne Moyles,
Alice Dohnalkova, Terry J Beveridge,
In Seop Chang,
Byung Hong Kim,
Kyung Shik Kim, [......],
Eric A Hill,
Liang Shi,
Dwayne A Elias,
David W Kennedy,
Grigoriy Pinchuk,
Kazuya Watanabe,
Shun'ichi Ishii,
Bruce Logan,
Kenneth H Nealson,
Jim K Fredrickson
[show abstract]
[hide abstract]
ABSTRACT: Shewanella oneidensis MR-1 produced electrically conductive pilus-like appendages called bacterial nanowires in direct response to electron-acceptor limitation. Mutants deficient in genes for c-type decaheme cytochromes MtrC and OmcA, and those that lacked a functional Type II secretion pathway displayed nanowires that were poorly conductive. These mutants were also deficient in their ability to reduce hydrous ferric oxide and in their ability to generate current in a microbial fuel cell. Nanowires produced by the oxygenic phototrophic cyanobacterium Synechocystis PCC6803 and the thermophilic, fermentative bacterium Pelotomaculum thermopropionicum reveal that electrically conductive appendages are not exclusive to dissimilatory metal-reducing bacteria and may, in fact, represent a common bacterial strategy for efficient electron transfer and energy distribution.
Proceedings of the National Academy of Sciences 08/2006; 103(30):11358-63. · 9.68 Impact Factor
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Yuri A. Gorby,
Svetlana Yanina,
Jeffrey S. McLean,
Kevin M. Rosso,
Dianne Moyles,
Alice Dohnalkova, Terry J. Beveridge,
In Seop Chang,
Byung Hong Kim,
Kyung Shik Kim, [......],
Eric A. Hill,
Liang Shi,
Dwayne A. Elias,
David W. Kennedy,
Grigoriy Pinchuk,
Kazuya Watanabe,
Shun’ichi Ishii,
Bruce Logan,
Kenneth H. Nealson,
Jim K. Fredrickson
[show abstract]
[hide abstract]
ABSTRACT: Shewanella oneidensis MR-1 produced electrically conductive pilus-like appendages called bacterial nanowires in direct response to electron-acceptor
limitation. Mutants deficient in genes for c-type decaheme cytochromes MtrC and OmcA, and those that lacked a functional Type II secretion pathway displayed nanowires
that were poorly conductive. These mutants were also deficient in their ability to reduce hydrous ferric oxide and in their
ability to generate current in a microbial fuel cell. Nanowires produced by the oxygenic phototrophic cyanobacterium Synechocystis PCC6803 and the thermophilic, fermentative bacterium Pelotomaculum thermopropionicum reveal that electrically conductive appendages are not exclusive to dissimilatory metal-reducing bacteria and may, in fact,
represent a common bacterial strategy for efficient electron transfer and energy distribution.
Proceedings of the National Academy of Sciences 07/2006; 103(30):11358-11363. · 9.68 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: Abstract Cytoplasmic inclusions surrounded by a bilayer membrane were seen in thin sections, negatively stained and freeze-fractured preparations of Shewanella putrefaciens. Cells harvested from the late exponential and early stationary phase showed a higher number of these vesicles than bacteria isolated from early exponential or late stationary phase. Chemical dyes for polyphosphate or poly-β-hydroxybutyrate did not stain the material enclosed within these vesicles. Elemental analysis of the material indicated that the content was organic in nature and might be a protein. HPLC analysis of the material showed that it was probably not a carbon source, nor an electron acceptor used by S. putrefaciens.
FEMS Microbiology Letters 01/2006; 139(1):63 - 69. · 2.04 Impact Factor
