T Hata

Kyoto University, Kyoto, Kyoto-fu, Japan

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Publications (331)775.94 Total impact

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    ABSTRACT: BACKGROUND AND AIM: Rejection of liver grafts is a difficult issue that has not been resolved. Preoperative replacement of liver cells in the graft with cells from the intended recipient may attenuate rejection. We investigated whether preoperative transplantation of recipient BMCs to the donor replaced liver allograft cells and attenuated rejection. METHODS: We used a rat model of allogeneic liver transplantation (LT) from DA to LEW rats. In BMC group, DA rats received BMC transplants from LacZ-transgenic Lewis rats at 1 week before LT. In Control group, DA rats received no preoperative treatment. We evaluated graft damage at 7 d after LT and the survival of the recipient rats. RESULTS: Rats in the BMC group experienced prolonged survival that was abrogated by the administration of gadolinium chloride to donors at 24 h before LT. Serum concentrations of total bilirubin and hyaluronic acid on day 7 were significantly lower in the BMC group, and histopathological analyses revealed that rejection of the liver graft was attenuated. X-gal staining and Immunohistostaining of the liver graft revealed that BMCs engrafted in the sinusoidal space differentiated into Kupffer cells. CONCLUSIONS: Preoperative transplantation of recipient BMCs to liver transplant donors replaced donor KCs and attenuated post-LT rejection, indicating that this strategy may increase the success of liver transplantation. This article is protected by copyright. All rights reserved.
    Journal of Gastroenterology and Hepatology 12/2014; · 3.33 Impact Factor
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    ABSTRACT: BACKGROUND AND AIM: Rejection of liver grafts is a difficult issue that has not been resolved. Preoperative replacement of liver cells in the graft with cells from the intended recipient may attenuate rejection. We investigated whether preoperative transplantation of recipient BMCs to the donor replaced liver allograft cells and attenuated rejection. METHODS: We used a rat model of allogeneic liver transplantation (LT) from DA to LEW rats. In BMC group, DA rats received BMC transplants from LacZ-transgenic Lewis rats at 1 week before LT. In Control group, DA rats received no preoperative treatment. We evaluated graft damage at 7 d after LT and the survival of the recipient rats. RESULTS: Rats in the BMC group experienced prolonged survival that was abrogated by the administration of gadolinium chloride to donors at 24 h before LT. Serum concentrations of total bilirubin and hyaluronic acid on day 7 were significantly lower in the BMC group, and histopathological analyses revealed that rejection of the liver graft was attenuated. X-gal staining and Immunohistostaining of the liver graft revealed that BMCs engrafted in the sinusoidal space differentiated into Kupffer cells. CONCLUSIONS: Preoperative transplantation of recipient BMCs to liver transplant donors replaced donor KCs and attenuated post-LT rejection, indicating that this strategy may increase the success of liver transplantation. This article is protected by copyright. All rights reserved.
    Journal of Gastroenterology and Hepatology 12/2014; · 3.33 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND AND AIM: Rejection of liver grafts is a difficult issue that has not been resolved. Preoperative replacement of liver cells in the graft with cells from the intended recipient may attenuate rejection. We investigated whether preoperative transplantation of recipient BMCs to the donor replaced liver allograft cells and attenuated rejection. METHODS: We used a rat model of allogeneic liver transplantation (LT) from DA to LEW rats. In BMC group, DA rats received BMC transplants from LacZ-transgenic Lewis rats at 1 week before LT. In Control group, DA rats received no preoperative treatment. We evaluated graft damage at 7 d after LT and the survival of the recipient rats. RESULTS: Rats in the BMC group experienced prolonged survival that was abrogated by the administration of gadolinium chloride to donors at 24 h before LT. Serum concentrations of total bilirubin and hyaluronic acid on day 7 were significantly lower in the BMC group, and histopathological analyses revealed that rejection of the liver graft was attenuated. X-gal staining and Immunohistostaining of the liver graft revealed that BMCs engrafted in the sinusoidal space differentiated into Kupffer cells. CONCLUSIONS: Preoperative transplantation of recipient BMCs to liver transplant donors replaced donor KCs and attenuated post-LT rejection, indicating that this strategy may increase the success of liver transplantation. This article is protected by copyright. All rights reserved.
    Journal of Gastroenterology and Hepatology 12/2014; · 3.33 Impact Factor
  • Placenta 10/2014; 35(10):A9. · 3.12 Impact Factor
  • Placenta 10/2014; 35(10):A8. · 3.12 Impact Factor
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    ABSTRACT: The investigators present their experience with normal fetal cardiac structures and congenital heart anomalies reconstructed using 4-dimensional color Doppler with glass-body rendering mode and spatiotemporal image correlation. Two normal fetuses and 6 fetuses with congenital heart anomalies (1 case each of ventricular septal defect, Ebstein's anomaly, hypoplastic left heart syndrome, and ductus arteriosus aneurysm and 2 of double-outlet right ventricle) at 26 to 36 weeks' gestation were studied using 4-dimensional color Doppler with glass-body rendering mode. In normal fetal hearts, blood flow through the 4 cardiac chambers and crisscross arrangements of the pulmonary artery and aorta were clearly recognized. In the fetus with a ventricular septal defect, significant shunt flow through the defect between the left and right ventricles was evident. In the fetus with Ebstein's anomaly, giant tricuspid regurgitant flow was noted. In the fetus with hypoplastic left heart syndrome, large tricuspid regurgitant flow was identified. In the fetuses with double-outlet right ventricles, large aortas and small pulmonary arteries leaving the right ventricles in parallel were clearly shown. In the fetus with a ductus arteriosus aneurysm, an enlarged ductus arteriosus following the pulmonary artery was clearly depicted. In conclusion, fetal 4-dimensional color Doppler may assist in the evaluation of spatial relations between the great vessels and both ventricles and differences in the sizes of the great vessels and cardiac chambers.
    The American Journal of Cardiology 08/2014; · 3.21 Impact Factor
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    Gastrointestinal Endoscopy 05/2014; · 5.21 Impact Factor
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    ABSTRACT: To investigate oxidative stress (OS)-mediated damage and the behavior of extracellular matrices in various rat models because shear stress with portal hypertension and cold ischemia/warm reperfusion injury trigger the liver regeneration cascade after surgery. These injuries also cause fatal liver damage. Rats were divided into four groups according to the surgery performed: control; hepatectomy with 40% liver remnant (60% hepatectomy); orthotopic liver transplantation (OLT) with whole liver graft (100% OLT); and split OLT (SOLT) with 40% graft (40% SOLT). Survival was evaluated. Blood and liver samples were collected at 6 h after surgery. Biochemical and histopathological examinations were performed. OS-induced damage, 4-hydroxynonenal, ataxia-telangiectasia mutated kinase, histone H2AX, phosphatidylinositol 3-kinase (PI3K) and Akt were evaluated by western blotting. Behavior of extracellular matrices, matrix metalloproteinase (MMP)-9, MMP-2, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 were also evaluated by western blotting and zymography. Although 100% OLT survived, 60% hepatectomy and 40% SOLT showed poor survival. Histopathological, immunohistological, biochemical and protein assays revealed that 60% hepatectomy, 100% OLT and 40% SOLT showed liver damage. PI3K and Akt were decreased in 60% hepatectomy and 40% SOLT. For protein expression, 40% SOLT showed differences in MMP-9, MMP-2 and TIMP-2. TIMP-1 showed differences in 60% hepatectomy and 40% SOLT. For protein activity, MMP-9 demonstrated significant differences in 60% hepatectomy, 100% OLT and 40% SOLT. Under conditions with an insufficient liver remnant, prevention of OS-induced damage via the Akt/PI3K pathway may be key to improve the postoperative course. MMP-9 may be also a therapeutic target after surgery.
    World journal of hepatology. 02/2014; 6(2):72-84.
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    ABSTRACT: An insufficient remnant in extended hepatectomy and small-for-size graft in liver transplantation are critical matters in the field of liver surgery, and reliable and reproducible animal models that can provide clinically relevant and reliable data are needed. We herein describe our detailed surgical procedures for performing 70 % hepatectomy in pigs, and discuss the critical anatomical features, key techniques and pitfalls based on our experience. The porcine liver is divided into four lobes. The right lateral lobe (RLL) accounts for 30 % of the liver volume. Important points, such as selective temporal clamping of the arterial branch, confirmation of a related demarcation line, a two-step process to skeletonize Glisson's capsules during liver resection and selective ligation of the portal venous branch to the right medial lobe without inducing any subtle injuries to Glisson's capsules from the RLL to common bile duct, are discussed.
    Surgery Today 02/2014; · 0.96 Impact Factor
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    ABSTRACT: We present a case of vertical, bridging uterine synechia in the central part of the uterine cavity diagnosed by conventional two-dimensional (2D) sonography and HDlive at 29 weeks and 5 days of gestation. 2D sonography showed a uterine synechia located vertically in the central part of the uterine cavity, dividing the lower uterine cavity into two parts. HDlive clearly revealed triangular, vertical, and bridging uterine synechia with foot protrusion and umbilical cord prolapse in the lower uterine cavity. The low, liquor-filled amniotic cavity was divided by a thin membrane. MRI confirmed the vertical structure dividing the uterus with umbilical cord prolapse. Emergency cesarean section was performed at 37 weeks and 1 day of gestation because of onset of labor, and a female infant weighing 2,380 g was delivered with an umbilical artery pH of 7.25, and Apgar score of 6/9 at 1 and 5 min, respectively. The vertical, bridging synechia in the central part of uterine cavity was confirmed during the operation.
    Journal of Medical Ultrasonics 01/2014; · 0.64 Impact Factor
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    ABSTRACT: Objective To assess the frequency of fetal facial expressions at 20–24 weeks of gestation using four-dimensional (4D) ultrasonography and to determine whether there was any correlation between facial expression and gestational age (20–34 weeks). Methods The facial expressions of 23 healthy fetuses were examined using 4D ultrasound at 20–24 weeks. Each fetus was recorded continuously for 15 minutes. The frequencies of mouthing, yawning, smiling, tongue expulsion, scowling, sucking, and blinking were assessed and the data combined with those expressions observed at 25–34 weeks of gestation in two previous studies (n = 34) to determine the correlation between gestational age and each of the facial expressions. Results Mouthing was significantly more frequent than the other six facial expressions at 20–24 weeks (P < 0.05). Yawning was significantly more frequent than smiling, scowling, and blinking (P < 0.05), and sucking was significantly more frequent than smiling, scowling, and blinking (P < 0.05). The frequency of yawning, smiling, tongue expulsion, scowling, and blinking increased with gestational age (P < 0.05). Mouthing movement and sucking frequencies remained constant between 20 and 34 weeks. Conclusion Frequencies of complicated facial expressions such as smiling and scowling may increase with advancing gestation owing to the development of the fetal brain and central nervous system.
    International Journal of Gynecology & Obstetrics. 01/2014;
  • Toshiyuki Hata, Uiko Hanaoka, Masato Mashima
    Journal of Medical Ultrasonics 01/2014; 41(1). · 0.64 Impact Factor
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    Ultrasound in Obstetrics and Gynecology 10/2013; 42(s1). · 3.56 Impact Factor
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    ABSTRACT: We present a case of intra-amniotic umbilical vein varix with thrombosis using conventional two-dimensional (2D) sonography, power Doppler, three-dimensional (3D) HD-flow, and HDlive at 35 weeks of gestation. 2D sonography showed a large banana-like umbilical cord enlargement (100 × 43.3 × 45.9 mm) including umbilical vein varix (maximum vein diameter = 25.5 mm) with massive thrombosis. Power Doppler and 3D HD-flow revealed bidirectional turbulent blood flow inside the varix. The HDlive clearly demonstrated fragile massive thrombosis inside the varix. Elective cesarean section was performed on the same day in order to avoid additional risks of umbilical cord complications and umbilical venous embolism. A male infant weighing 2501 g was delivered with an umbilical artery pH of 7.334, and Apgar score of 8/9 at 1 and 5 min, respectively. The macroscopic and microscopic findings revealed umbilical cord vein varix with thrombosis. On the basis of the laboratory date of the neonate, the diagnosis of consumptive coagulopathy was made. However, the neonate followed a favorable course after delivery.
    Placenta 09/2013; · 3.12 Impact Factor
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    ABSTRACT: Upshaw-Schulman syndrome (USS) involves a congenital deficiency of von Willebrand factor-cleaving metalloprotease (ADAMTS13) activity due to gene mutations. Female patients develop overt thrombotic thrombocytopenic purpura (TTP) caused by a decline of ADAMTS13 activity in pregnancy. A 23-year-old nulliparous Japanese woman died due to severe, rapid progression of TTP with intrauterine fetal death at 20 weeks of gestation after its onset, even though she underwent intensive treatment which included plasma exchange. She had a history of idiopathic thrombocytopenic purpura at the age of 3 years. The patient's ADAMTS13 activity was of very low level. It should be borne in mind that there is the possibility of rapidly progressive fulminant USS during pregnancy.
    Journal of Obstetrics and Gynaecology Research 08/2013; · 0.84 Impact Factor
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    ABSTRACT: In developing therapeutic alternatives to liver transplantation, we have used the strategy of applying a small intestinal segment as a scaffold for hepatocyte transplantation and also as a portocaval shunt (PCS) system to address both liver dysfunction and portal hypertension. The aim of this study was to investigate the feasibility of such an intestinal segment in animal models. Hepatocytes isolated from luciferase-transgenic Lewis rats were transplanted into jejunal segments of wild-type Lewis rats with mucosa removal without PCS application. Luciferase-derived luminescence from transplanted hepatocytes was stably detected for 30 days. Then, we performed autologous hepatocyte transplantation into the submucosal layer of an isolated and vascularized small intestinal segment in pigs. Transplanted hepatocytes were isolated from the resected left-lateral lobe of the liver. On day 7, hepatocyte clusters and bile duct-like structures were observed histologically. To create an intestinal PCS system in pigs, an auto-graft of the segmental ileum and interposing vessel graft were anastomosed to the portal vein trunk and inferior vena cava. However, thrombi were observed in vessels of the intestinal PCSs. We measured the correlation between infusion pressure and flow volume in whole intestines ex vivo in both species and found that the high pressure corresponding to portal hypertension was still insufficient to maintain the patency of the intestinal grafts. In conclusion, we demonstrated the feasibility of the small intestine as a scaffold for hepatocyte transplantation in rat and pig models, but PCS using an intestinal graft failed to maintain patency in a pig model.
    Organogenesis 08/2013; 9(4). · 2.28 Impact Factor
  • Toshiyuki Hata, Shinji Uemoto, Eiji Kobayashi
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    ABSTRACT: Organ grafts developed in the xenogeneic pig scaffold are expected to resolve most issues of donor safety and ethical concerns about living-donor liver transplantation in Japan. We have been working on so-called "Yamaton" projects to develop transplantable organs using genetically engineered pigs. Our goal is to produce chimeric livers with human parenchyma in such pigs. The Yamaton-Liver project demonstrated the proof of concept by showing that rat-mouse chimeric livers could develop in mice and be successfully transplanted into syngeneic or allogeneic rats. Under conventional immunosuppression, the transplanted livers showed long-term function and protection against rejection. Because chimeric liver grafts have xenogeneic components, additional strategies, such as humanization of pig genes, induction of hematopoietic chimeras in donors, and replacement of pig endothelial cells with human ones, might be required in clinical use. Our projects still need to overcome various hurdles but can bring huge benefits to patients in the future.
    Organogenesis 07/2013; 9(4). · 2.28 Impact Factor
  • Archives of Gynecology 07/2013; · 0.91 Impact Factor
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    ABSTRACT: Our objective is to describe our experience with reconstruction of normal fetal cardiac structures and congenital heart anomalies using the 3-D HDlive rendering mode with spatiotemporal image correlation (STIC). Four normal fetuses and three fetuses with congenital heart anomalies (Ebstein's anomaly, hypoplastic left heart syndrome and tetralogy of Fallot) at 25-35 wk of gestation were studied using the 3-D HDlive rendering mode with STIC. In normal fetuses, the natural appearance of the dynamic motion of the foramen ovale flap and both atrioventricular valves was clearly visualized in real time in the four-chamber view. Moreover, new, realistic sensations of each leaflet of atrioventricular valves and semilunar valves of the pulmonary artery were obtained in the en face view of both atrioventricular valves and great vessels. In the case of Ebstein's anomaly, the procedure rendered the natural and anatomically realistic appearance of significantly low attachment of the tricuspid valve and atrialized portion of the right ventricle. In hypoplastic left heart syndrome, thickened tricuspid and dysplastic pulmonary valves were clearly revealed. In tetralogy of Fallot, an overriding aorta and ventricular septal defect were realistically depicted. The 3-D HDlive rendering mode with STIC provides entirely new visual experiences for obstetricians and pediatric cardiologists owing to the anatomically realistic depiction of normal and abnormal fetal cardiac structures of the beating heart.
    Ultrasound in medicine & biology 06/2013; · 2.46 Impact Factor
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    Gastroenterology 05/2013; 144(5):S-1030. · 12.82 Impact Factor

Publication Stats

2k Citations
775.94 Total Impact Points

Institutions

  • 2010–2013
    • Kyoto University
      • • Department of Hepato-pancreato-biliary Surgery and Transplantation
      • • Graduate School of Medicine / Faculty of Medicine
      Kyoto, Kyoto-fu, Japan
    • Jichi Medical University
      • Department of Development of Advanced Treatment
      Totigi, Tochigi, Japan
  • 1999–2013
    • Kagawa University
      • • Department of Perinatology and Gynecology
      • • Faculty of Medicine
      Takamatu, Kagawa, Japan
    • Tango Central Hospital
      Kioto, Kyōto, Japan
  • 2012
    • Mayo Foundation for Medical Education and Research
      • Department of Neuroscience
      Rochester, MI, United States
  • 2009
    • Osaka City University
      Ōsaka, Ōsaka, Japan
  • 1983–2009
    • Shimane University
      • Department of Obstetrics and Gynecology
      Izumo, Shimane-ken, Japan
  • 1993
    • West Georgia Obstetrics and Gynecology
      Georgetown, Georgia, United States
  • 1991–1993
    • Baylor College of Medicine
      • Department of Obstetrics and Gynecology
      Houston, TX, United States