T. Matsumoto

Kyoto University, Kyoto, Kyoto-fu, Japan

Are you T. Matsumoto?

Claim your profile

Publications (23)46.59 Total impact

  • Fujio Hyodo · Yoko Takematsu · T. Matsumoto · Yoko Inui · Takao Itioka ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Despite the recognition of the functional role of Hymenoptera (ants, bees and wasps) and Isoptera (termites) in tropical ecosystems, their detailed feeding habits are not well known. To examine the feeding habits of these groups, we measured nitrogen (N) and carbon (C) stable isotope ratios (d15N and d13C) of hymenopterans (12 families, C16 genera and C32 species) and isopterans (one family and 10 species) collected in a tropical rain forest, Sarawak, Malaysia. We compared the isotopic signatures of these insects to those previously reported for other consumers collected in the same forest. The d15N and d13C values of these insects overlapped with those of the other consumers, indicating that they have access to diverse C and N sources in the forest. The d15N values of ants and termites indicated that their feeding habits range along a continuum from herbivory (i.e. dependent on honeydew and nectar) to predation and from wood-feeders to soil-feeders, respectively. In addition, the d15N values of wasps varied greatly from -0.1% (Braconidae sp.) to 8.6% (Bembix sp.), suggesting that their feeding habits also range from omnivory to predation. The ant species Camponotus gigas had d13C values similar to those of invertebrate detritivores and omnivores rather than to those of invertebrate herbivores, although the diet of this species consists mostly of honeydew. This discrepancy suggests that the ant uses carbohydrates as an energy source, the isotopic signatures of which are not well retained in the body tissues. Values of both d15N and d13Cof the predatory army ant Leptogenys diminuta and the soilfeeding termite Dicuspiditermes nemorosus did not differ significantly, indicating that both trophic level and the humification of feeding substrates can increase the isotopic signatures of terrestrial consumers.
    Insectes Sociaux 08/2011; 58(3):417–426. DOI:10.1007/s00040-011-0159-9 · 1.02 Impact Factor
  • T. Matsumoto · T. Itioka · Sk. Yamane · K. Momose ·
    [Show abstract] [Hide abstract]
    ABSTRACT: We examined the effect of traditional swidden agriculture on biodiversity using the litter arthropod top predator, the army ant Aenictus (Hymenoptera: Formicidae) as an indicator species in and around an intact tropical rain forest in Sarawak, Borneo, Malaysia. We compared the encounter rates with Aenictus colonies among five forest types: continuous primary forest, isolated primary forest, old secondary forest (>20years elapsed after abandonment of the fields), young secondary forest (5years after abandonment), and new fallow (2years after abandonment) by intensive area searching in 2003 and 2005. In total, seven Aenictus species (15 colonies) and six Aenictus species (11 colonies) were encountered in 2003 and 2005, respectively. The encounter rates were the highest in continuous and isolated primary forests, intermediate in old and young secondary forests, and the lowest in new fallow. Year and the interaction between year and forest type were not significant. That is, abundance of top predators, which is rare and likely to be vulnerable to disturbance, has never fully recovered even 20years after the termination of cultivation. We discuss forest management strategies to sustain biological diversity.
    Biodiversity and Conservation 11/2009; 18(12):3139-3151. DOI:10.1007/s10531-009-9632-4 · 2.37 Impact Factor
  • Source
    K. Maekawa · T. Matsumoto · CA Nalepa ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Recent phylogenetic studies indicating that termites are eusocial cockroaches closely related to the genus Cryptocercus have generated fresh interest in wood-feeding Blattaria. Here we summarize the social biology of the wood-feeding genus Salganea (Blaberidae: Panesthiinae). As in Cryptocercus, Salganea exhibits long term, biparental care that includes the defense and feeding of young nymphs. Unlike Cryptocercus, however, Salganea is iteroparous: most studied species reproduce periodically over their lifetime. These divergent reproductive strategies are likely related to parental costs associated with their differing reproductive modes: Cryptocercus is oviparous, while Salganea is ovoviviparous. The pattern of parental investment associated with ovoviviparous reproduction may be one reason why Salganea and other ovoviviparous wood-feeding cockroach lineages did not evolve eusociality.
    Insectes Sociaux 04/2008; 55(2):107-114. DOI:10.1007/s00040-008-0997-2 · 1.02 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The evolution of fungus-growing termites is supposed to have started in the African rain forests with multiple invasions of semi-arid habitats as well as multiple invasions of the Oriental region. We used sequences of the mitochondrial COII gene and Bayesian dating to investigate the time frame of the evolution of Macrotermes, an important genus of fungus-growing termites. We found that the genus Macrotermes consists of at least 6 distantly related clades. Furthermore, the COII sequences suggested some cryptic diversity within the analysed African Macrotermes species. The dates calculated with the COII data using a fossilized termite mound to calibrate the clock were in good agreement with dates calculated with COI sequences using the split between Locusta and Chortippus as calibration point which supports the consistency of the calibration points. The clades from the Oriental region dated back to the early Tertiary. These estimates of divergence times suggested that Macrotermes invaded Asia during periods with humid climates. For Africa, many speciation events predated the Pleistocene and fall in range of 6-23 million years ago. These estimates suggest that savannah-adapted African clades radiated with the spread of the semi-arid ecosystems during the Miocene. Apparently, events during the Pleistocene were of little importance for speciation within the genus Macrotermes. However, further investigations are necessary to increase the number of taxa for phylogenetic analysis.
    Molecular Phylogenetics and Evolution 11/2007; 45(1):239-50. DOI:10.1016/j.ympev.2007.07.007 · 3.92 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Eight polymorphic microsatellite markers were developed from morphotype L1 of the Southeast Asian army ant, Aenictus laeviceps in Borneo, and characterized for morphotypes L1 and L2. The number of alleles per locus ranged from nine to 24 (average 15) in morphotype L1 and two to 16 (average 9.8) in L2. Observed heterozygosity ranged from 0.75 to 0.96 in morphotype L1 and from 0.08 to 0.96 in L2. All loci were in Hardy–Weinberg equilibrium. One pair of loci showed linkage disequilibrium in morphotype L1. All markers successfully amplified in morphotype S, and some markers amplified in other Aenictus species.
    Molecular Ecology Notes 03/2007; 7(6):984 - 986. DOI:10.1111/j.1471-8286.2007.01746.x · 2.38 Impact Factor
  • H. Katoh · T. Matsumoto · T. Miura ·
    [Show abstract] [Hide abstract]
    ABSTRACT: In social insects, caste-specific characters develop in the postembryonic differentiation processes. However, the mechanisms of caste-specific organ development have yet to be elucidated. In order to obtain insights into the relationship between caste differentiation and the regulation of organ development, we determined the caste-developmental pathway and observed compound-eye development accompanying alate differentiation in the dry-wood termite, Neotermes koshunensis. As previously reported in other Neotermes, this species has a linear caste-developmental pathway, comprising six larval- and two nymphal-instar stages. Although the apparent eye formation occurs during the last nymphal stages, just prior to the imaginal molt, individuals possess eye primordia from the first larval-instar stage. The outer morphological structure of the eye was observed from the third larval-instar stage. The detailed differentiation of cells constituting ommatidia appeared to occur in relatively young larval instars (fourth stage), although the pigmentation of pigment cells and detailed structural formation of ommatidia occurred during the final stage of alate development, i.e., during the late second nymphal-instar stage. This suggests that eye development is arrested in the larval stages, and then resumed during the late nymphal stage to complete functional eye formation, which is required for nuptial flight. In comparison to major hemimetabolous insects, which possess functional compound eyes even at the first instar larva, this termite species shows the heterochronic shift in terms of compound-eye development.
    Insectes Sociaux 01/2007; 54(1):11-19. DOI:10.1007/s00040-006-0900-y · 1.02 Impact Factor
  • Source
    R Cornette · S Koshikawa · M Hojo · T Matsumoto · T Miura ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Termites are eusocial insects with a well-defined caste system, which is an example of polyphenism. This polyphenism is based on hormonally controlled differential gene expression. In the damp-wood termite Hodotermopsis sjostedti, we induced differentiation into the soldier caste by using juvenile hormone analogue treatment. We then investigated specific gene expression, which appeared during the hormonal response and triggered caste differentiation, using fluorescent differential display. A candidate cDNA sequence with similarity to cytochromes P450, CYP6AM1, was characterized and its transcript shown to be repressed between 1 and 3 days after hormone treatment. CYP6AM1 was specifically expressed in the fat body of pseudergates and soldiers. The putative function of this P450 is discussed with respect to the caste differentiation system.
    Insect Molecular Biology 05/2006; 15(2):235-44. DOI:10.1111/j.1365-2583.2006.00632.x · 2.59 Impact Factor
  • N. Ijichi · H. Shibao · T. Miura · T. Matsumoto · T. Fukatsu ·
    [Show abstract] [Hide abstract]
    ABSTRACT: We investigated the morphological characters of normal nymphs, soldier nymphs and developing embryos of a social aphid, Colophina arma, which has a sterile soldier caste in the first instar. Morphometric analysis revealed that normal nymphs and soldier nymphs were clearly distinguishable on the basis of several morphological characters. At late embryonic stages, normal embryos and soldier embryos were also distinguishable morphologically. The younger the embryonic stages, the smaller the morphological differences between them. In young embryos of less than 600m in body length, normal embryos and soldier embryos were no more distinguishable, suggesting that the onset of soldier differentiation occurs at an early embryonic stage. Through the embryonic development, morphological differentiation of soldier caste proceeded gradually: forelegs and midlegs were exaggerated, and growth of mouthpart was suppressed, which resulted in the soldier morphology specialized for attacking behavior. On the basis of these results, developmental aspects in soldier differentiation of C. arma were compared with those of Pseudoregma bambucicola, a social aphid with a first instar soldier caste of independent evolutionary origin. Ecological and evolutionary relevance of the differences between the two social aphids was discussed.
    Insectes Sociaux 04/2005; 52(2):177-185. DOI:10.1007/s00040-004-0792-7 · 1.02 Impact Factor
  • A Gotoh · S Sameshima · K Tsuji · T Matsumoto · T Miura ·
    [Show abstract] [Hide abstract]
    ABSTRACT: We here show an example of morphological novelties, which have evolved from insect wings into the specific structures controlling social behaviour in an ant species. Most ant colonies consist of winged queen(s) and wingless workers. In the queenless ponerine ant Diacamma sp. from Japan, however, all female workers have a pair of small thoracic appendages, called "gemmae", which are homologous to the forewings and acts as an organ regulating altruism expression. Most workers, whose gemmae are clipped off by other colony members, become nonreproductive helpers, while only a single individual with complete gemmae becomes functionally reproductive. We examined histologically the development of gemmae, and compared it with that of functional wings in males. Female larvae had well-developed wing discs for both fore- and hindwings. At pupation, however, the wing discs started to evaginate and later degenerate. The hindwing discs completely degenerated, while the degeneration of forewing discs was incomplete, leading to the formation of gemmae. The degeneration process involved apoptotic cell death as confirmed by TUNEL assay. In addition, glandular cells differentiated from the epithelial cells of the forewing buds after completion of pupation. The mechanism of developmental transition from wing to gemma can be regarded as an evolutionary gain of new function, which can be seen in insect appendages and vertebrate limbs.
    Development Genes and Evolution 03/2005; 215(2):69-77. DOI:10.1007/s00427-004-0456-7 · 2.44 Impact Factor
  • T Tsuchida · R Koga · X.Y. Meng · T Matsumoto · T Fukatsu ·
    [Show abstract] [Hide abstract]
    ABSTRACT: The pea aphid U-type symbiont (PAUS) was investigated to characterize its microbiological properties. Fluorescence in situ hybridization (FISH) and electron microscopy revealed that PAUS was a rod-shaped bacterium found in three different locations in the body of the pea aphid Acyrthosiphon pisum: sheath cells, secondary mycetocytes, and hemolymph. Artificial transfer experiments revealed that PAUS could establish stable infection and vertical transmission when introduced into uninfected pea aphids. When 28 aphid species collected in Japan were subjected to a diagnostic PCR assay, four species of the subfamily Aphidinae (Aphis citricola, Aphis nerii, Macrosiphum avenae, and Uroleucon giganteus) and a species of the subfamily Pemphiginae (Colopha kansugei) were identified to be PAUS-positive. The sporadic incidences of PAUS infection without reflecting the aphid phylogeny can be best explained by occasional horizontal transfers of the symbiont across aphid lineages.
    Microbial Ecology 02/2005; 49(1):126-33. DOI:10.1007/s00248-004-0216-2 · 2.97 Impact Factor
  • N. Lo · O. Kitade · T. Miura · R. Constantino · T. Matsumoto ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Relationships among genera in the termite family Rhinotermitidae and their relationship to the families Termitidae and Serritermitidae were investigated based on analysis of three mitochondrial genes: COI, COII and 16S rDNA. Maximum Parsimony (MP) bootstrap analysis of each of these genes indicated a low level of phylogenetic incongruence between them, and thus they were combined and analysed by MP and Bayesian analysis. Six main lineages were clearly identified, however relationships among these were not well defined. Tentative support was found for the Rhinotermitid genera Coptotermes, Heterotermes and Reticulitermes being the sister group to the Termitidae, rendering the Rhinotermitidae paraphyletic. The species Serritermes serrifer and Glossotermes oculatus were found to group with strong support, in agreement with the recent transfer of the latter species to the family Serritermitidae based on morphological characteristics. No support was found for the Rhinotermitidae being paraphyletic with respect to the Serritermitidae. A number of disagreements were found between the molecular tree and traditional classifications of genera within subfamilies.
    Insectes Sociaux 10/2004; 51(4):365-371. DOI:10.1007/s00040-004-0759-8 · 1.02 Impact Factor
  • Source
    T. Miura · S. Koshikawa · M. Machida · T. Matsumoto ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Termite colonies are comprised of several types of castes that differentiate throughout postembryonic development. In termopsid termites (family Termopsidae), alates are normally differentiated from apterous instars by two nymphal instar stages and three moulting events. Here, we report that of the rotten-wood termite Hodotermopsis sjostedti. There is only a single nymphal instar between the pseudergate and alate stages. During the annual alate production season in June/July, we observed some nymphs with small wing buds which were dorsally swollen. Those nymphal individuals subsequently moulted into alates through only a single moult. We examined their histology and internal morphology and observed that the folding pattern of the newly formed wings was very different from that seen in second stage nymphs of the closely related species Zootermopsis nevadensis. The newly formed wings of H. sjostedti are formed inside the relatively smaller wing buds and therefore must be folded in a complicated manner. Our observations revealed that the tips of the folded wings were elongated and bent, such that they overlapped in the median plane. We suggest that heterochronic evolutionary change accounts for the compression of nymphal development into a single instar. We also suggest that this probably occurred at either the individual or colony level in this species.
    Insectes Sociaux 08/2004; 51(3). DOI:10.1007/s00040-003-0736-2 · 1.02 Impact Factor
  • T. Matsumoto · T. Itioka · T. Nishida · T. Inoue ·
    [Show abstract] [Hide abstract]
    ABSTRACT:   In 1980, two species of parasitoid wasps (Aphytis yanonensis DeBach et Rosen and Coccobius fulvus Compere et Annecke) were introduced to Japan from China as biological control agents to combat the arrowhead scale (Unaspis yanonensis Kuwana). These introductions represent one of the most successful projects in the history of biological control in Japan. To examine whether density dependent parasitism was inevitable for success of biological control, we tried to detect temporal and spatial density dependence in parasitism rates using time-series data of scale density, as well as parasitism, over a 16-year period. The work was conducted in a Satsuma mandarin orange (Citrus unshiu Marc.) orchard in which we previously demonstrated that the system appeared to have stabilized after a decline in scale density following the introduction of the parasitoids. Earlier work also indicated that C. fulvus contributes most to the reduction in, and the stability of, scale density. In this study, we examined: (1) the relationship, on a whole-orchard basis, between scale density and the rates of parasitism by A. yanonensis, C. fulvus, and a combination of the two species; (2) whether parasitism was positively correlated to scale density on a single-tree basis among generations and (3) whether spatial density dependence was detectable within generations on an individual-tree basis. Parasitism by A. yanonensis was temporally density-dependent on scale population density at the whole-orchard level, while parasitism by C. fulvus was not. Parasitism by A. yanonensis or by C. fulvus was rarely positively correlated to scale density at the single-tree level, and spatial density-dependence was hardly detected at all at this level. Most analyses of combined parasitism rates were similar to rates of parasitism by C. fulvus alone. Contrary to conventional wisdom of biological control theory, this study demonstrates that density dependence is not necessarily detected, even in a system in which a natural enemy has long held pest density stable at low levels.
    Journal of Applied Entomology 05/2004; 128(4):267 - 272. DOI:10.1111/j.1439-0418.2004.00819.x · 1.65 Impact Factor
  • J Garcia · K Maekawa · T Miura · R Constantino · T Matsumoto ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Termites, distributed mainly in tropical regions, are eusocial insects that live in highly organized and integrated colonies. It is considered that eusociality is favored by high intracolony relatedness, since inclusive fitness is increased. It has been argued that in the case of termites, high genetic relatedness within the colony is achieved by alternating cycles of inbreeding and outbreeding within the population, where a pair of reproductives that are unrelated but a product of intense inbreeding, produces highly related offspring. However, population inbreeding, intercolony genetic relationships, and the hierarchical structure of termite populations have been under studied. In this study, we used the amplified fragment length polymorphism (AFLP) technique to analyze population genetic diversity and genetic distance between termite nests of Nasutitermes nigriceps and between nests of N. corniger in Guatemala to clarify the degree of genetic relatedness among different colonies within a termite population. Genetic distance was analyzed at both long- and short-distance-scales. Genetic diversity was determined within termite populations and genetic distance was estimated between colony nests. A Mantel test was applied to the values of geographic and genetic distances. For N. nigriceps a total of 181 AFLP fragments were obtained from the three primer combinations resulting in 26 (14%) polymorphic bands. For N. corniger a total of 199 AFLP fragments were obtained from the three primer combinations resulting in 39 (19%) polymorphic bands. At both distance scales, a clear correlation was not found between geographic and genetic distances. These low values of genetic diversity suggest that a genetic bottle neck could have occurred in the termite populations. The weak relationship between genetic and geographic distances could be the result of the low genetic diversity within the termite populations, or the result of sampling a non-substructured population.
    Sociobiology 01/2003; 41(3-3):663-672. · 0.37 Impact Factor
  • H Katoh · T Miura · K Maekawa · N Shinzato · T Matsumoto ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Fungus-growing termites have a mutualistic relationship with their cultivated fungi. To improve understanding of genetic aspects of this relationship, we examined molecular markers in the fungus-growing termite Odontotermes formosanus and its fungi Termitomyces spp. from the Ryukyu Archipelago. Based on the polymorphic band patterns obtained from arbitrarily primed polymerase chain reaction methods, we constructed cladograms for related colonies of the termites and fungi. The resulting trees indicated that the termites display little genetic variation among the colonies, while the symbiotic fungi consist of two major genetic types. In addition, molecular phylogenetic trees of the symbiotic fungi based on internal transcribed spacer and 18S rDNA suggested that these two types of fungi are different species. We also demonstrated that the fungi comprising the fruiting bodies and fungus combs are identical, and that fungus combs are probably a monoculture within a single termite colony. Our results indicate that horizontal transmission of symbiotic fungi among termite colonies occurred during the evolutionary history of this symbiosis.
    Molecular Ecology 09/2002; 11(8):1565-72. DOI:10.1046/j.1365-294X.2002.01535.x · 6.49 Impact Factor
  • Source
    S Koshikawa · T Matsumoto · T Miura ·
    [Show abstract] [Hide abstract]
    ABSTRACT: To clarify the allometric development of body parts accompanying soldier differentiation in termites, we measured 16 body parts of soldiers, presoldiers, pseudergates (workers), nymphs and larvae of the damp-wood termite Hodotermopsis japonica. Principal component analysis (PCA) was performed using these parameters, which revealed that differentiation into soldiers differed distinctly from development into adult (reproductive) individuals. In particular, the anterior body parts enlarged during development of soldiers. Similarly, elongation of the apical portion of both mandibles was noted during soldier differentiation. X-ray analysis of mandibles revealed sclerotization of the soldier mandibles after differentiation into terminal soldiers. These morphological changes during soldier differentiation are associated with changes in their roles within the colony. Through soldier differentiation, the morphology of this caste of termite becomes functionally suited for attacking predators, and unsuitable for feeding on wood using their mandibles. Based on these data, we suggest that there must be some morphogenetic factors leading caste specific morphology such as soldier mandibles.
    Insectes Sociaux 08/2002; 49(3):245-250. DOI:10.1007/s00040-002-8309-8 · 1.02 Impact Factor
  • Source
    M. Machida · O. Kitade · T. Matsumoto ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Summary Trophallaxis is one of a variety of nutritional tactics that enable termites to conserve and recycle nitrogenous compounds. In this study, we designed three experiments to reveal the function and importance of proctodeal trophallaxis in the Japanese damp-wood termite Hodotermopsis japonica. First, our observations showed that younger instars and soldiers tended to behave as recipients of proctodeal fluid, while older instars acted mainly as donors. Secondly, nitrogen-deficient groups of termite individuals were introduced to groups of nourished individuals; results indicate that the frequency of proctodeal trophallaxis was significantly higher than in control groups. Additionally, the nitrogen content in the proctodeal fluid of nitrogen-deficient individuals increased after trophallactic interactions with well-nourished individuals. Finally, we set up three groups reared with different concentrations of nitrogenous compounds (amino acids) in their diet, to investigate the correlation between the frequency of trophallactic behavior and the protein concentration of proctodeal fluid. As expected, proctodeal fluid of low protein content was frequently exchanged among individuals in nitrogen-poor conditions, while high-protein proctodeal fluid was transferred less frequently under nitrogen-rich conditions. These results suggest that termites have plasticity of trophallactic behavior in response to their nutritional conditions, and trophallaxis contributes to the nutritional homeostasis of colonies.
    Insectes Sociaux 03/2001; 48(1):52-56. DOI:10.1007/PL00001745 · 1.02 Impact Factor
  • Source
    S Koshikawa · T Matsumoto · T Miura ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Regressive molts from nymphs to pseudergates were observed in Hodotermopsis japonica in experimental groups composed of nymphs alone. This is the first study of the genus Hodotermopsis, and the regressive molt in this species was unique for Termopsidae because wing bud biting prior to the regressive molt was not observed. The thoracic morphologies of pseudergates derived from nymphs were different from those of normal pseudergates: the posterior margin of the metanotum was trapezoidal. It was suggested that the regressive molt occasionally occurs in this species, and is one of the features caused by the flexibility of caste differentiation in termites.
    Sociobiology 01/2001; 38(3):495-500. · 0.37 Impact Factor
  • Source
    T Miura · T Matsumoto ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Nasute termites belonging to the subfamily Nasutitermitinae, have a soldier caste that possesses a frontal projection (nasus) on the head, from which defensive substances are secreted. In the course of caste differentiation of the processional nasute termite Hospitalitermes medioflavus, the most dynamic morphogenesis occurs in the stage of moulting from male minor worker to presoldier (the stage preceding the soldier stage). We examined the presumptive nasus epithelium in minor workers and determined that the nasus develops rapidly just prior to the moulting to presoldiers. The rapid growth is associated with two folding layers of cuticle and epithelium, which we termed the soldier-nasus disc, and resembles the imaginal discs found in holometabolous insects.
    Proceedings of the Royal Society B: Biological Sciences 07/2000; 267(1449):1185-9. DOI:10.1098/rspb.2000.1127 · 5.05 Impact Factor
  • Source
    N Shinzato · T Matsumoto · I Yamaoka · T Oshima · A Yamagishi ·
    [Show abstract] [Hide abstract]
    ABSTRACT: A phylogenetic analysis of the sequences of 60 clones of archaeal small-subunit rRNA genes amplified from the termite Reticulitermes speratus revealed that most of them (56 clones) clustered in the genus Methanobrevibacter. Three clones were classified in the order Thermoplasmales. The Methanobrevibacter-related symbionts were detected by in situ hybridization analysis.
    Applied and Environmental Microbiology 02/1999; 65(2):837-840. · 3.67 Impact Factor

Publication Stats

543 Citations
46.59 Total Impact Points


  • 2007-2011
    • Kyoto University
      • Graduate School of Human and Environmental Studies
      Kyoto, Kyoto-fu, Japan
    • Chiba University
      Tiba, Chiba, Japan
    • Air University
      Maxwell, California, United States
  • 2008
    • The Open University of Japan
      • Faculty of Liberal Arts
      Edo, Tōkyō, Japan
  • 1995-2006
    • The University of Tokyo
      • • College of Art and Science & Graduate School of Arts and Sciences
      • • Department of Applied Life Sciences
      Edo, Tōkyō, Japan
  • 2004
    • Nagoya University
      • Graduate School of Bio-Agricultural Sciences
      Nagoya, Aichi, Japan