T Matsumoto

Kyoto University, Kyoto, Kyoto-fu, Japan

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Publications (26)59.92 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Despite the recognition of the functional role of Hymenoptera (ants, bees and wasps) and Isoptera (termites) in tropical ecosystems, their detailed feeding habits are not well known. To examine the feeding habits of these groups, we measured nitrogen (N) and carbon (C) stable isotope ratios (d15N and d13C) of hymenopterans (12 families, C16 genera and C32 species) and isopterans (one family and 10 species) collected in a tropical rain forest, Sarawak, Malaysia. We compared the isotopic signatures of these insects to those previously reported for other consumers collected in the same forest. The d15N and d13C values of these insects overlapped with those of the other consumers, indicating that they have access to diverse C and N sources in the forest. The d15N values of ants and termites indicated that their feeding habits range along a continuum from herbivory (i.e. dependent on honeydew and nectar) to predation and from wood-feeders to soil-feeders, respectively. In addition, the d15N values of wasps varied greatly from -0.1% (Braconidae sp.) to 8.6% (Bembix sp.), suggesting that their feeding habits also range from omnivory to predation. The ant species Camponotus gigas had d13C values similar to those of invertebrate detritivores and omnivores rather than to those of invertebrate herbivores, although the diet of this species consists mostly of honeydew. This discrepancy suggests that the ant uses carbohydrates as an energy source, the isotopic signatures of which are not well retained in the body tissues. Values of both d15N and d13Cof the predatory army ant Leptogenys diminuta and the soilfeeding termite Dicuspiditermes nemorosus did not differ significantly, indicating that both trophic level and the humification of feeding substrates can increase the isotopic signatures of terrestrial consumers.
    Insectes Sociaux 01/2011; 58(3):417–426. · 1.33 Impact Factor
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    ABSTRACT: The evolution of fungus-growing termites is supposed to have started in the African rain forests with multiple invasions of semi-arid habitats as well as multiple invasions of the Oriental region. We used sequences of the mitochondrial COII gene and Bayesian dating to investigate the time frame of the evolution of Macrotermes, an important genus of fungus-growing termites. We found that the genus Macrotermes consists of at least 6 distantly related clades. Furthermore, the COII sequences suggested some cryptic diversity within the analysed African Macrotermes species. The dates calculated with the COII data using a fossilized termite mound to calibrate the clock were in good agreement with dates calculated with COI sequences using the split between Locusta and Chortippus as calibration point which supports the consistency of the calibration points. The clades from the Oriental region dated back to the early Tertiary. These estimates of divergence times suggested that Macrotermes invaded Asia during periods with humid climates. For Africa, many speciation events predated the Pleistocene and fall in range of 6-23 million years ago. These estimates suggest that savannah-adapted African clades radiated with the spread of the semi-arid ecosystems during the Miocene. Apparently, events during the Pleistocene were of little importance for speciation within the genus Macrotermes. However, further investigations are necessary to increase the number of taxa for phylogenetic analysis.
    Molecular Phylogenetics and Evolution 11/2007; 45(1):239-50. · 4.07 Impact Factor
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    ABSTRACT: Eight polymorphic microsatellite markers were developed from morphotype L1 of the Southeast Asian army ant, Aenictus laeviceps in Borneo, and characterized for morphotypes L1 and L2. The number of alleles per locus ranged from nine to 24 (average 15) in morphotype L1 and two to 16 (average 9.8) in L2. Observed heterozygosity ranged from 0.75 to 0.96 in morphotype L1 and from 0.08 to 0.96 in L2. All loci were in Hardy–Weinberg equilibrium. One pair of loci showed linkage disequilibrium in morphotype L1. All markers successfully amplified in morphotype S, and some markers amplified in other Aenictus species.
    Molecular Ecology Notes 03/2007; 7(6):984 - 986. · 2.38 Impact Factor
  • H. Katoh, T. Matsumoto, T. Miura
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    ABSTRACT: In social insects, caste-specific characters develop in the postembryonic differentiation processes. However, the mechanisms of caste-specific organ development have yet to be elucidated. In order to obtain insights into the relationship between caste differentiation and the regulation of organ development, we determined the caste-developmental pathway and observed compound-eye development accompanying alate differentiation in the dry-wood termite, Neotermes koshunensis. As previously reported in other Neotermes, this species has a linear caste-developmental pathway, comprising six larval- and two nymphal-instar stages. Although the apparent eye formation occurs during the last nymphal stages, just prior to the imaginal molt, individuals possess eye primordia from the first larval-instar stage. The outer morphological structure of the eye was observed from the third larval-instar stage. The detailed differentiation of cells constituting ommatidia appeared to occur in relatively young larval instars (fourth stage), although the pigmentation of pigment cells and detailed structural formation of ommatidia occurred during the final stage of alate development, i.e., during the late second nymphal-instar stage. This suggests that eye development is arrested in the larval stages, and then resumed during the late nymphal stage to complete functional eye formation, which is required for nuptial flight. In comparison to major hemimetabolous insects, which possess functional compound eyes even at the first instar larva, this termite species shows the heterochronic shift in terms of compound-eye development.
    Insectes Sociaux 01/2007; 54(1):11-19. · 1.33 Impact Factor
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    ABSTRACT: Termites are eusocial insects with a well-defined caste system, which is an example of polyphenism. This polyphenism is based on hormonally controlled differential gene expression. In the damp-wood termite Hodotermopsis sjostedti, we induced differentiation into the soldier caste by using juvenile hormone analogue treatment. We then investigated specific gene expression, which appeared during the hormonal response and triggered caste differentiation, using fluorescent differential display. A candidate cDNA sequence with similarity to cytochromes P450, CYP6AM1, was characterized and its transcript shown to be repressed between 1 and 3 days after hormone treatment. CYP6AM1 was specifically expressed in the fat body of pseudergates and soldiers. The putative function of this P450 is discussed with respect to the caste differentiation system.
    Insect Molecular Biology 05/2006; 15(2):235-44. · 3.04 Impact Factor
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    Entomological Science 12/2005; 8(4):379-387. · 0.98 Impact Factor
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    ABSTRACT: We investigated the morphological characters of normal nymphs, soldier nymphs and developing embryos of a social aphid, Colophina arma, which has a sterile soldier caste in the first instar. Morphometric analysis revealed that normal nymphs and soldier nymphs were clearly distinguishable on the basis of several morphological characters. At late embryonic stages, normal embryos and soldier embryos were also distinguishable morphologically. The younger the embryonic stages, the smaller the morphological differences between them. In young embryos of less than 600m in body length, normal embryos and soldier embryos were no more distinguishable, suggesting that the onset of soldier differentiation occurs at an early embryonic stage. Through the embryonic development, morphological differentiation of soldier caste proceeded gradually: forelegs and midlegs were exaggerated, and growth of mouthpart was suppressed, which resulted in the soldier morphology specialized for attacking behavior. On the basis of these results, developmental aspects in soldier differentiation of C. arma were compared with those of Pseudoregma bambucicola, a social aphid with a first instar soldier caste of independent evolutionary origin. Ecological and evolutionary relevance of the differences between the two social aphids was discussed.
    Insectes Sociaux 04/2005; 52(2):177-185. · 1.33 Impact Factor
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    ABSTRACT: We here show an example of morphological novelties, which have evolved from insect wings into the specific structures controlling social behaviour in an ant species. Most ant colonies consist of winged queen(s) and wingless workers. In the queenless ponerine ant Diacamma sp. from Japan, however, all female workers have a pair of small thoracic appendages, called "gemmae", which are homologous to the forewings and acts as an organ regulating altruism expression. Most workers, whose gemmae are clipped off by other colony members, become nonreproductive helpers, while only a single individual with complete gemmae becomes functionally reproductive. We examined histologically the development of gemmae, and compared it with that of functional wings in males. Female larvae had well-developed wing discs for both fore- and hindwings. At pupation, however, the wing discs started to evaginate and later degenerate. The hindwing discs completely degenerated, while the degeneration of forewing discs was incomplete, leading to the formation of gemmae. The degeneration process involved apoptotic cell death as confirmed by TUNEL assay. In addition, glandular cells differentiated from the epithelial cells of the forewing buds after completion of pupation. The mechanism of developmental transition from wing to gemma can be regarded as an evolutionary gain of new function, which can be seen in insect appendages and vertebrate limbs.
    Development Genes and Evolution 03/2005; 215(2):69-77. · 1.70 Impact Factor
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    ABSTRACT: The pea aphid U-type symbiont (PAUS) was investigated to characterize its microbiological properties. Fluorescence in situ hybridization (FISH) and electron microscopy revealed that PAUS was a rod-shaped bacterium found in three different locations in the body of the pea aphid Acyrthosiphon pisum: sheath cells, secondary mycetocytes, and hemolymph. Artificial transfer experiments revealed that PAUS could establish stable infection and vertical transmission when introduced into uninfected pea aphids. When 28 aphid species collected in Japan were subjected to a diagnostic PCR assay, four species of the subfamily Aphidinae (Aphis citricola, Aphis nerii, Macrosiphum avenae, and Uroleucon giganteus) and a species of the subfamily Pemphiginae (Colopha kansugei) were identified to be PAUS-positive. The sporadic incidences of PAUS infection without reflecting the aphid phylogeny can be best explained by occasional horizontal transfers of the symbiont across aphid lineages.
    Microbial Ecology 02/2005; 49(1):126-33. · 3.28 Impact Factor
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    ABSTRACT: The termite soldier is unique because of its defensive task in a colony. In Nasutitermitinae (family Termitidae), soldiers use in their defense frontal glands, which contain various chemical substances. To isolate the gene products related to the chemical defense, we compared the sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profiles of soldier heads with those of workers of the nasute termite Nasutitermes takasagoensis. We identified a 26-kDa soldier-specific protein (Ntsp1) that exists most abundantly in the dorsal head including the frontal gland. We determined the N-terminal amino acid sequence of Ntsp1, and then cloned the Ntsp1 cDNA by rapid amplification of the cDNA ends-polymerase chain reaction (RACE-PCR). A putative signal peptide was detected upstream of the N-terminus and the Ntsp1 protein showed sequence homologies with known insect secretory carrier proteins, which bind to hydrophobic ligands such as juvenile hormone, suggesting that Ntsp1 belongs to this class of proteins. Northern blot analysis confirmed that the expression level of Ntsp1 was high only in the soldier head. In addition, the localization of Ntsp1 expression was limited in epithelial cells of the frontal gland reservoir, suggesting that this protein binds to some terpenoid(s) preserved in the frontal gland reservoir.
    Insect Biochemistry and Molecular Biology 01/2005; 35(4):347-354. · 3.23 Impact Factor
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    ABSTRACT: Relationships among genera in the termite family Rhinotermitidae and their relationship to the families Termitidae and Serritermitidae were investigated based on analysis of three mitochondrial genes: COI, COII and 16S rDNA. Maximum Parsimony (MP) bootstrap analysis of each of these genes indicated a low level of phylogenetic incongruence between them, and thus they were combined and analysed by MP and Bayesian analysis. Six main lineages were clearly identified, however relationships among these were not well defined. Tentative support was found for the Rhinotermitid genera Coptotermes, Heterotermes and Reticulitermes being the sister group to the Termitidae, rendering the Rhinotermitidae paraphyletic. The species Serritermes serrifer and Glossotermes oculatus were found to group with strong support, in agreement with the recent transfer of the latter species to the family Serritermitidae based on morphological characteristics. No support was found for the Rhinotermitidae being paraphyletic with respect to the Serritermitidae. A number of disagreements were found between the molecular tree and traditional classifications of genera within subfamilies.
    Insectes Sociaux 10/2004; 51(4):365-371. · 1.33 Impact Factor
  • T. Matsumoto, T. Itioka, T. Nishida, T. Inoue
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    ABSTRACT:   In 1980, two species of parasitoid wasps (Aphytis yanonensis DeBach et Rosen and Coccobius fulvus Compere et Annecke) were introduced to Japan from China as biological control agents to combat the arrowhead scale (Unaspis yanonensis Kuwana). These introductions represent one of the most successful projects in the history of biological control in Japan. To examine whether density dependent parasitism was inevitable for success of biological control, we tried to detect temporal and spatial density dependence in parasitism rates using time-series data of scale density, as well as parasitism, over a 16-year period. The work was conducted in a Satsuma mandarin orange (Citrus unshiu Marc.) orchard in which we previously demonstrated that the system appeared to have stabilized after a decline in scale density following the introduction of the parasitoids. Earlier work also indicated that C. fulvus contributes most to the reduction in, and the stability of, scale density. In this study, we examined: (1) the relationship, on a whole-orchard basis, between scale density and the rates of parasitism by A. yanonensis, C. fulvus, and a combination of the two species; (2) whether parasitism was positively correlated to scale density on a single-tree basis among generations and (3) whether spatial density dependence was detectable within generations on an individual-tree basis. Parasitism by A. yanonensis was temporally density-dependent on scale population density at the whole-orchard level, while parasitism by C. fulvus was not. Parasitism by A. yanonensis or by C. fulvus was rarely positively correlated to scale density at the single-tree level, and spatial density-dependence was hardly detected at all at this level. Most analyses of combined parasitism rates were similar to rates of parasitism by C. fulvus alone. Contrary to conventional wisdom of biological control theory, this study demonstrates that density dependence is not necessarily detected, even in a system in which a natural enemy has long held pest density stable at low levels.
    Journal of Applied Entomology 05/2004; 128(4):267 - 272. · 1.56 Impact Factor
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    T. Miura, S. Koshikawa, M. Machida, T. Matsumoto
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    ABSTRACT: Termite colonies are comprised of several types of castes that differentiate throughout postembryonic development. In termopsid termites (family Termopsidae), alates are normally differentiated from apterous instars by two nymphal instar stages and three moulting events. Here, we report that of the rotten-wood termite Hodotermopsis sjostedti. There is only a single nymphal instar between the pseudergate and alate stages. During the annual alate production season in June/July, we observed some nymphs with small wing buds which were dorsally swollen. Those nymphal individuals subsequently moulted into alates through only a single moult. We examined their histology and internal morphology and observed that the folding pattern of the newly formed wings was very different from that seen in second stage nymphs of the closely related species Zootermopsis nevadensis. The newly formed wings of H. sjostedti are formed inside the relatively smaller wing buds and therefore must be folded in a complicated manner. Our observations revealed that the tips of the folded wings were elongated and bent, such that they overlapped in the median plane. We suggest that heterochronic evolutionary change accounts for the compression of nymphal development into a single instar. We also suggest that this probably occurred at either the individual or colony level in this species.
    Insectes Sociaux 01/2004; 51(3). · 1.33 Impact Factor
  • Sociobiology 01/2003; 41(3):663-672. · 0.58 Impact Factor
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    ABSTRACT: Fungus-growing termites have a mutualistic relationship with their cultivated fungi. To improve understanding of genetic aspects of this relationship, we examined molecular markers in the fungus-growing termite Odontotermes formosanus and its fungi Termitomyces spp. from the Ryukyu Archipelago. Based on the polymorphic band patterns obtained from arbitrarily primed polymerase chain reaction methods, we constructed cladograms for related colonies of the termites and fungi. The resulting trees indicated that the termites display little genetic variation among the colonies, while the symbiotic fungi consist of two major genetic types. In addition, molecular phylogenetic trees of the symbiotic fungi based on internal transcribed spacer and 18S rDNA suggested that these two types of fungi are different species. We also demonstrated that the fungi comprising the fruiting bodies and fungus combs are identical, and that fungus combs are probably a monoculture within a single termite colony. Our results indicate that horizontal transmission of symbiotic fungi among termite colonies occurred during the evolutionary history of this symbiosis.
    Molecular Ecology 09/2002; 11(8):1565-72. · 6.28 Impact Factor
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    S Koshikawa, T Matsumoto, T Miura
    Insectes Sociaux 08/2002; 49(3):245-250. · 1.33 Impact Factor
  • K Maekawa, M Kon, K Araya, T Matsumoto
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    ABSTRACT: Molecular phylogenetic relationships among 25 species of the wood-feeding cockroach belonging to the genus Salganea Stål (Panesthiinae; Blaberidae) in Southeast Asia were analyzed based on the DNA sequence of the complete mitochondrial cytochrome oxidase II (COII) gene. Most basal relationships among species of Salganea are poorly resolved by both neighbor-joining and nonweighted parsimony analyses, suggesting the possibility of a hard polytomy due to a rapid and potentially simultaneous radiation early in the history of the genus. For more apical relationships, however, some interesting phylogenetic relationships were recognized. The monophyly of the two species groups, morio and foveolata, the former of which is distributed mainly in the Sunda lands (containing the Malay Peninsula, Sumatra, Java, and Borneo), whereas the latter is Sulawesi endemic, was strongly supported. Based on the inferred phylogenetic patterns and recent palaeogeographic scenario for Southeast Asia, it is suggested that a radiation of Salganea species occurred in Southeast Asia presumably in the early Tertiary, and several barriers against dispersal and gene flow, such as the formation of straits or high mountains, have arisen from the middle Tertiary.
    Journal of Molecular Evolution 01/2002; 53(6):651-9. · 2.15 Impact Factor
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    M. Machida, O. Kitade, T. Matsumoto
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    ABSTRACT: Summary Trophallaxis is one of a variety of nutritional tactics that enable termites to conserve and recycle nitrogenous compounds. In this study, we designed three experiments to reveal the function and importance of proctodeal trophallaxis in the Japanese damp-wood termite Hodotermopsis japonica. First, our observations showed that younger instars and soldiers tended to behave as recipients of proctodeal fluid, while older instars acted mainly as donors. Secondly, nitrogen-deficient groups of termite individuals were introduced to groups of nourished individuals; results indicate that the frequency of proctodeal trophallaxis was significantly higher than in control groups. Additionally, the nitrogen content in the proctodeal fluid of nitrogen-deficient individuals increased after trophallactic interactions with well-nourished individuals. Finally, we set up three groups reared with different concentrations of nitrogenous compounds (amino acids) in their diet, to investigate the correlation between the frequency of trophallactic behavior and the protein concentration of proctodeal fluid. As expected, proctodeal fluid of low protein content was frequently exchanged among individuals in nitrogen-poor conditions, while high-protein proctodeal fluid was transferred less frequently under nitrogen-rich conditions. These results suggest that termites have plasticity of trophallactic behavior in response to their nutritional conditions, and trophallaxis contributes to the nutritional homeostasis of colonies.
    Insectes Sociaux 01/2001; 48(1):52-56. · 1.33 Impact Factor
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    S Koshikawa, T Matsumoto, T Miura
    Sociobiology 01/2001; 38(3):495-500. · 0.58 Impact Factor
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    T Miura, T Matsumoto
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    ABSTRACT: Nasute termites belonging to the subfamily Nasutitermitinae, have a soldier caste that possesses a frontal projection (nasus) on the head, from which defensive substances are secreted. In the course of caste differentiation of the processional nasute termite Hospitalitermes medioflavus, the most dynamic morphogenesis occurs in the stage of moulting from male minor worker to presoldier (the stage preceding the soldier stage). We examined the presumptive nasus epithelium in minor workers and determined that the nasus develops rapidly just prior to the moulting to presoldiers. The rapid growth is associated with two folding layers of cuticle and epithelium, which we termed the soldier-nasus disc, and resembles the imaginal discs found in holometabolous insects.
    Proceedings of the Royal Society B: Biological Sciences 07/2000; 267(1449):1185-9. · 5.68 Impact Factor

Publication Stats

396 Citations
59.92 Total Impact Points


  • 2004–2011
    • Kyoto University
      • • Graduate School of Human and Environmental Studies
      • • Graduate School of Agriculture / Faculty of Agriculture
      Kyoto, Kyoto-fu, Japan
  • 2007
    • Air University
      Maxwell, California, United States
  • 1995–2006
    • The University of Tokyo
      • College of Art and Science & Graduate School of Arts and Sciences
      Tokyo, Tokyo-to, Japan